TrcrA.01212.b

Cytosolic malate dehydrogenase, putative

CENTER ID: TrcrA.01212.b
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.01212.b.B1.GE35144 full length 1 332
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053506937.10
RefSeq: XP_805517.1
UniProt: Q9NJT1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFFEGACAKV VVSGAAGQVG YALLPLIAGG RMLGPNQHLQ LNLLDIEPAM KCLEGIRAEL MDCAFPLLDR VVITHKPAVA FENVDIAILC GSFPAKPGTL RRDLLQKNAA IFSEHGRLLG ELASKDCHVC VVGNPVNTNA LVLLNASNGK IKPKNVSALT RLDHNRSLAL VAERANAHVR DVKNCIIWGN HSGTQVPDVN SATVRGVPVR EAIKDDAYFD GEFMTTVQQR GYEIIRWRGN SSALSAANAA VDQVHDWVLG TPTGTHVSMA VYSDGNPYGV PPGLVFSFPV TCSGGEWQFV ENACVTPSVA KHLAATTKEL EEERSESLSL AL
NT Sequence
atgttttttg aaggtgcatg cgcgaaggtg gtcgtctcgg gggcggccgg ccaagttggg tacgcattac tccccctcat tgccggcggc cgcatgttgg gtcccaatca acatctgcag ctgaacctgc tcgatattga gcccgccatg aaatgtcttg aggggatacg tgcggaactc atggactgtg ccttcccact gctggaccgt gttgtcatca cgcacaaacc ggcggtggcc tttgagaatg ttgatatcgc cattctctgt ggctcgtttc cggccaaacc cggcacgctg cggagggatc tcctgcagaa aaatgccgca attttttcag aacatgggcg tcttttgggt gaattggcca gtaaagactg ccacgtgtgt gttgtgggca atcctgtcaa caccaatgcc cttgttcttt tgaatgcctc gaacgggaaa atcaagccga agaacgtgtc ggcgcttacg cgactggacc acaaccgttc gcttgcgctt gtggcagagc gagccaatgc tcatgttcga gatgtcaaga actgtattat ttggggaaac cacagcggta cacaagtccc ggatgttaac agcgccactg tgagaggcgt ccctgtgcga gaagcgataa aggatgacgc gtactttgat ggagaattta tgacaactgt gcagcaacgc gggtatgaga ttattcggtg gcgcggcaac tcttcggctc tttctgccgc aaatgccgct gttgaccaag tgcacgactg ggttcttggt acgccaacgg ggacacatgt ttccatggct gtgtattccg atggtaaccc atacggtgtg ccaccaggcc ttgtcttctc cttccccgtg acatgcagcg gaggtgagtg gcagtttgtc gagaatgcgt gtgttacgcc gagtgtggcg aaacatttgg ctgccaccac caaagagttg gaagaggaac ggtcggagtc gctgtctttg gcattgtaa
Details for TrcrA.01212.b.B1.GE35144
HARVESTED ON: 4/24/2012
SEQUENCED ON: 4/24/2012
EXPECTED MW: 36kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 18
PERCENT COVERAGE: 80
Validated AA Sequence
MAHHHHHHMF FEGACAQFTI PSSHRTVGVF GADMYLYVAA APIKASALAW ATTCLKLPDG RPIFGVVNYG PSSVTDSEDG VRVVAHEIAH ALGFAVEIMR KLNMLKEVKG VRGKAKVPQV SSPKTAEKTR EHFNCVSATS VELEDEGGEG TALSHWKRRN AKDELMAGHS GIGYYTSLTM AALEDTGLYK ANWGMEEPMS WGNNSGCALL KEKSVMNGVT KYPEMFCTAE TTLISCTSDR LELGHCTIEL YDAPLPPQYQ YFSNPKLGGS PGF
Validated NT Sequence
ttnnnnnnnt taagaaggag anataccatg gctcaccacc accaccacca tatgtttttt gaaggtgcat gcgctcagtt tacaataccc agctctcacc gcacagtggg tgttttcggt gcggacatgt acttgtatgt cgcagccgcc cctataaagg cttccgctct tgcgtgggct actacatgct taaaattacc cgatggacgc cctattttcg gagtggtgaa ttatggcccg agctctgtca ctgattcgga ggacggtgtt cgtgttgtag cgcatgagat tgcacatgct cttgggtttg ctgtggagat aatgagaaaa ctcaatatgt taaaagaggt taagggtgta cgtggcaaag caaaggtgcc gcaggtgtcg tcacccaaga cggcggagaa gactcgcgag cacttcaact gcgtgagcgc taccagcgtg gagctggagg acgagggtgg ggaaggaact gcgttgtcgc attggaagag acgcaacgca aaggacgagc tgatggctgg tcattccgga atcggctact acacgtccct gacaatggct gctttggagg acaccgggct ttacaaggcc aattggggaa tggaggaacc gatgtcatgg ggcaacaact ccggctgcgc gctgctgaag gagaaaagcg tgatgaatgg cgtcacaaag tatccagaga tgttttgcac cgctgaaaca actcttattt catgcacatc ggatcgtctg gagctggggc actgtacaat agaactctac gacgctcccc ttcccccaca atatcagtac ttttcgaatc ccaaacttgg cggttcgccc ggtttttaat ggacttctgc ccttacattc gaccgttcgc caacactggg tgcaatgggg aacgcaaccc tcatgcgtgg gantcgctgt ctttggcatt gtaagtgagt aananaggan cnnnnnnnnn nnnnnnnnnn naaaaannnn nnnnnnnnnn nnnnnnnnnc cncnnnnnnn nnnannnnan nnnnnnnnna nnnttttttn tcnnc
Expected Protein Sequence
MAHHHHHHMF FEGACAKVVV SGAAGQVGYA LLPLIAGGRM LGPNQHLQLN LLDIEPAMKC LEGIRAELMD CAFPLLDRVV ITHKPAVAFE NVDIAILCGS FPAKPGTLRR DLLQKNAAIF SEHGRLLGEL ASKDCHVCVV GNPVNTNALV LLNASNGKIK PKNVSALTRL DHNRSLALVA ERANAHVRDV KNCIIWGNHS GTQVPDVNSA TVRGVPVREA IKDDAYFDGE FMTTVQQRGY EIIRWRGNSS ALSAANAAVD QVHDWVLGTP TGTHVSMAVY SDGNPYGVPP GLVFSFPVTC SGGEWQFVEN ACVTPSVAKH LAATTKELEE ERSESLSLAL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtttt ttgaaggtgc atgcgcgaag gtggtcgtct cgggggcggc cggccaagtt gggtacgcat tactccccct cattgccggc ggccgcatgt tgggtcccaa tcaacatctg cagctgaacc tgctcgatat tgagcccgcc atgaaatgtc ttgaggggat acgtgcggaa ctcatggact gtgccttccc actgctggac cgtgttgtca tcacgcacaa accggcggtg gcctttgaga atgttgatat cgccattctc tgtggctcgt ttccggccaa acccggcacg ctgcggaggg atctcctgca gaaaaatgcc gcaatttttt cagaacatgg gcgtcttttg ggtgaattgg ccagtaaaga ctgccacgtg tgtgttgtgg gcaatcctgt caacaccaat gcccttgttc ttttgaatgc ctcgaacggg aaaatcaagc cgaagaacgt gtcggcgctt acgcgactgg accacaaccg ttcgcttgcg cttgtggcag agcgagccaa tgctcatgtt cgagatgtca agaactgtat tatttgggga aaccacagcg gtacacaagt cccggatgtt aacagcgcca ctgtgagagg cgtccctgtg cgagaagcga taaaggatga cgcgtacttt gatggagaat ttatgacaac tgtgcagcaa cgcgggtatg agattattcg gtggcgcggc aactcttcgg ctctttctgc cgcaaatgcc gctgttgacc aagtgcacga ctgggttctt ggtacgccaa cggggacaca tgtttccatg gctgtgtatt ccgatggtaa cccatacggt gtgccaccag gccttgtctt ctccttcccc gtgacatgca gcggaggtga gtggcagttt gtcgagaatg cgtgtgttac gccgagtgtg gcgaaacatt tggctgccac caccaaagag ttggaagagg aacggtcgga gtcgctgtct ttggcattgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat