TrcrA.01174.a

prostaglandin f synthase

CENTER ID: TrcrA.01174.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.01174.a.B1.GE35140 full length 1 339
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053511627.120
RefSeq: XP_810536.1
UniProt: Q4D831

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPGANVLSFK PLGGPVLGVS HRLPLRNGNS IPQCGFGTYR MTPTVAGAAV EYAIHCGFRH IDCAKAYDNQ NAIGEALQRV ISTGNLKREE LFLTSKLWPT DQHPIHVEKA CRETLAELRV DYLDLYLIHW PVVWNHSPHF KTDDEKYPKD ANGLPAVDDS VKLIDTWRAM CELVDRNLVR SVGLSNCSEK HINEVMSDGS LYAPVVNQIE LHPALVQRDL LNFHGANQIV TAAYSPLGMP SRFTHPDYKG LLSDASLQSI SEYSGFSVAR LLLNWNLDMH NVVIVRSTNK EHIRSNAKAS LYALSDPVRM ILDRFQERVG TIRTINPTDF TRGGGSFFD
NT Sequence
atgcccggtg cgaacgttct gagtttcaag ccactcgggg ggcctgtgct cggcgtgtcg catcggctac cgttgagaaa tggaaatagc attcctcagt gcggctttgg aacgtaccgc atgaccccca cagtggcagg agctgccgtc gaatatgcca tacattgtgg ttttcgacat attgattgtg ctaaggcata cgacaaccag aacgctattg gagaggcact tcaacgtgtg atatcaactg gcaatttgaa gcgtgaggaa ttgtttttga cgtcaaagct ttggcccaca gatcaacacc caattcacgt ggagaaggct tgtcgagaga cgttagcgga gctgcgtgtg gactaccttg acttgtatct tattcactgg ccagtggtgt ggaatcattc cccgcatttc aagaccgatg acgagaaata ccccaaagac gccaacggac ttcctgcggt tgatgatagt gtcaaactta tcgatacttg gagggctatg tgtgaacttg ttgatagaaa tttggtgcgt tcagttgggc tctcgaactg tagtgagaaa catattaacg aagttatgag tgatggaagt ctgtatgcac cggtggtaaa ccagattgaa cttcacccgg cgttggtcca gcgtgacctt cttaatttcc acggtgcaaa tcaaatagtg acagctgctt acagtcctct ggggatgcct tcaagattta cacacccgga ctacaagggt ttgttgtccg acgcatcatt acaatccatt tccgagtatt caggatttag cgtggctcgt ttactactaa actggaacct tgacatgcac aatgtcgtga ttgtgcgttc aaccaacaag gaacacatca gatcgaacgc caaggcctct ctttatgcac tctcggatcc cgtgagaatg atcctggacc gattccagga gcgtgtggga acaattcgta caatcaatcc gacggacttc actcgtggcg gagggagctt ctttgactga
Details for TrcrA.01174.a.B1.GE35140
HARVESTED ON: 4/10/2012
SEQUENCED ON: 4/11/2012
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 85
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHMPG ANVLSFKPLG GPVLGVSHRL PLRNGNSIPQ CGFGTYRMTP TVAGAAVEYA IHCGFRHIDC AKAYDNQNAI GEALQRVIST GNLKREELFL TSKLWPTDQH PIHVEKACRE TLAELRVDYL DLYLIHWPVV WNHSPHFKTD DEKYPKDANG LPAVDDSVKL IDTWRAMCEL VDRNLVRSVG LSNCSEKHIN EVMSDGSLYA PVVNQIELHP ALVQRDLLNF HGANQIVTAA YSPLGMPSRF THPDYKGLLS DASLQSISXY SGFSVARLLL NWNLDMHNVV IVRSTXXGTH XIERXGLSLC TLXSRENDXG XXXXXXXXXV XXXXXXXXXX XXXXXXX
Validated NT Sequence
tttgttnnnn ttaagaagga gatataccat ggctcaccac caccaccata tgcccggtgc gaacgttctg agtttcaagc cactcggggg gcctgtgctc ggcgtgtcgc atcggctacc gttgagaaat ggaaatagca ttcctcagtg cggctttgga acgtaccgca tgacccccac agtggcagga gctgccgtcg aatatgccat acattgtggt tttcgacata ttgattgtgc taaggcatac gacaaccaga acgctattgg agaggcactt caacgtgtga tatcaactgg caatttgaag cgtgaggaat tgtttttgac gtcaaagctt tggcccacag atcaacaccc aattcacgtg gagaaggctt gtcgagagac gttagcggag ctgcgtgtgg actaccttga cttgtatctt attcactggc cagtggtgtg gaatcattcc ccgcatttca agaccgatga cgagaaatac cccaaagacg ccaacggact tcctgcggtt gatgatagtg tcaaacttat cgatacttgg agggctatgt gtgaacttgt tgatagaaat ttggtgcgtt cagttgggct ctcgaactgt agtgagaaac atattaacga agttatgagt gatggaagtc tgtatgcacc ggtggtaaac cagattgaac ttcacccggc gttggtccag cgtgaccttc ttaatttcca cggtgcaaat caaatagtga cagctgctta cagtcctctg gggatgcctt cnagatttac acacccggac tacaagggtt tgttgtccga cgcatcatta caatccattt ccgantattc aggatttagc gtggctcgtt tactactaaa ctggaacctt gacatgcaca atgtcgtgat tgtgcgttca accnacnang gaacacatca natcgaacgn cnnggcctct ctttatgcac tctcgnatcc cgngagaatg atcntggnnc gannncnnnn cnnnnngnnn cnnncgtana nnanncnann tnnntcnngn nnnnnnntnn tnantnnnnn nannnnnnnn c
Expected Protein Sequence
MAHHHHHHMP GANVLSFKPL GGPVLGVSHR LPLRNGNSIP QCGFGTYRMT PTVAGAAVEY AIHCGFRHID CAKAYDNQNA IGEALQRVIS TGNLKREELF LTSKLWPTDQ HPIHVEKACR ETLAELRVDY LDLYLIHWPV VWNHSPHFKT DDEKYPKDAN GLPAVDDSVK LIDTWRAMCE LVDRNLVRSV GLSNCSEKHI NEVMSDGSLY APVVNQIELH PALVQRDLLN FHGANQIVTA AYSPLGMPSR FTHPDYKGLL SDASLQSISE YSGFSVARLL LNWNLDMHNV VIVRSTNKEH IRSNAKASLY ALSDPVRMIL DRFQERVGTI RTINPTDFTR GGGSFFD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccg gtgcgaacgt tctgagtttc aagccactcg gggggcctgt gctcggcgtg tcgcatcggc taccgttgag aaatggaaat agcattcctc agtgcggctt tggaacgtac cgcatgaccc ccacagtggc aggagctgcc gtcgaatatg ccatacattg tggttttcga catattgatt gtgctaaggc atacgacaac cagaacgcta ttggagaggc acttcaacgt gtgatatcaa ctggcaattt gaagcgtgag gaattgtttt tgacgtcaaa gctttggccc acagatcaac acccaattca cgtggagaag gcttgtcgag agacgttagc ggagctgcgt gtggactacc ttgacttgta tcttattcac tggccagtgg tgtggaatca ttccccgcat ttcaagaccg atgacgagaa ataccccaaa gacgccaacg gacttcctgc ggttgatgat agtgtcaaac ttatcgatac ttggagggct atgtgtgaac ttgttgatag aaatttggtg cgttcagttg ggctctcgaa ctgtagtgag aaacatatta acgaagttat gagtgatgga agtctgtatg caccggtggt aaaccagatt gaacttcacc cggcgttggt ccagcgtgac cttcttaatt tccacggtgc aaatcaaata gtgacagctg cttacagtcc tctggggatg ccttcaagat ttacacaccc ggactacaag ggtttgttgt ccgacgcatc attacaatcc atttccgagt attcaggatt tagcgtggct cgtttactac taaactggaa ccttgacatg cacaatgtcg tgattgtgcg ttcaaccaac aaggaacaca tcagatcgaa cgccaaggcc tctctttatg cactctcgga tcccgtgaga atgatcctgg accgattcca ggagcgtgtg ggaacaattc gtacaatcaa tccgacggac ttcactcgtg gcggagggag cttctttgac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat