SSGCID: Target Page

TrcrA.01169.c

serine/threonine protein phosphatase, putative

CENTER ID:	TrcrA.01169.c
ORGANISM:	Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.01169.c.B1.GE35139	full length		1	340

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tc00.1047053511881.20
RefSeq:	XP_805588.1
UniProt:	Q4CTY6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEGALPTGIA NKTIASPTTT GTAVAVPTPP GAVGEPNSFN VDELIQYVLQ GKPLSEPQVA KLCQKGKEVL EKEGNVHTVR APVTVCGDIH GQFHDLLELL KIGGLPPDTN YLFMGDYVDR GYYSVETVTL LLLFKIRYPE RVQILRGNHE SRQITQVYGF YDECVRKYGS ANVWKLLTDL FDYLPLAAVV ENEIFCLHGG LSPTLDSLAH IRSLERVQEV PHEGPMCDLL WSDPEDRDGW GISPRGAGFT FGADISEQFC HNNGLKMIAR AHQLVTDGYS WAHNDRLVTI FSAPNYCYRC GNLAGLLELD EHMNKCFFQF DPAPRRGEAQ VSKKTPDYFL

NT Sequence

atggaggggg cgttacctac tggaattgca aataaaacga tagcgtcacc cacaaccaca gggacggcag tggcggtccc cacaccaccg ggtgcggtgg gtgagcctaa cagtttcaac gtggatgaat tgattcagta tgttttacag ggaaaaccat tgagcgagcc acaggtggca aagctgtgcc agaaaggcaa ggaggttctg gagaaggagg ggaatgttca caccgtgcgt gcacccgtga ccgtatgtgg cgatatacat ggtcaatttc atgatctgct ggagctgctg aagattggtg gtcttccacc tgacacgaat tatttattta tgggcgacta cgttgaccgc ggttactaca gtgtggagac agtcacattg ttgttgcttt ttaagatacg atacccggaa cgggttcaga ttcttcgtgg taaccatgaa tcacgccaga ttacgcaggt ctacggcttt tacgatgagt gtgtgcgcaa atatggcagc gccaatgtgt ggaagttgct aaccgatctt tttgattact tgccgctggc agcggtagtg gaaaacgaga tcttttgcct tcacggtggt ctttctccca cgttggatag tctggcgcat attcgtagct tagagcgcgt ccaggaggtt ccccacgagg ggcccatgtg cgatttgttg tggtcggatc ctgaagaccg tgatgggtgg ggcatcagtc cacgtggggc cggctttact tttggcgccg acatctccga gcagttctgt cacaacaacg gcctcaagat gattgcacgg gcacatcagt tggtgacgga cggatacagc tgggcacata atgatcgtct cgtgacgata tttagcgcac caaattactg ctaccgttgc ggtaatcttg ccggattgct ggagctcgac gagcacatga ataaatgttt ttttcagttt gatcccgccc cgcgacgagg tgaggcgcag gtgtcgaaga aaacccctga ctactttctc tga

Details for TrcrA.01169.c.B1.GE35139

HARVESTED ON:	4/10/2012
SEQUENCED ON:	4/11/2012
EXPECTED MW:	38kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHME GALPTGIANK TIASPTTTGT AVAVPTPPGA VGEPNSFNVD ELIQYVLQGK PLSEPQVAKL CQKGKEVLEK EGNVHTVRAP VTVCGDIHGQ FHDLLELLKI GGLPPDTNYL FMGDYVDRGY YSVETVTLLL LFKIRYPERV QILRGNHESR QITQVYGFYD ECVRKYGSAN VWKLLTDLFD YLPLAAVVEN EIFCLHGGLS PTLDSLAHIR SLERVQEVPH EGPMCDLLWS DPEDRDGWGI SPRGAGFTFG ADISEQFCHN NGLKMIARAH QLVTDGYSWA HNDRLVTIFS APNYCYRCGN LAGLLELDEH MNKCFFQFDP APRRGEAQVS KKTPDYFL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggagg gggcgttacc tactggaatt gcaaataaaa cgatagcgtc acccacaacc acagggacgg cagtggcggt ccccacacca ccgggtgcgg tgggtgagcc taacagtttc aacgtggatg aattgattca gtatgtttta cagggaaaac cattgagcga gccacaggtg gcaaagctgt gccagaaagg caaggaggtt ctggagaagg aggggaatgt tcacaccgtg cgtgcacccg tgaccgtatg tggcgatata catggtcaat ttcatgatct gctggagctg ctgaagattg gtggtcttcc acctgacacg aattatttat ttatgggcga ctacgttgac cgcggttact acagtgtgga gacagtcaca ttgttgttgc tttttaagat acgatacccg gaacgggttc agattcttcg tggtaaccat gaatcacgcc agattacgca ggtctacggc ttttacgatg agtgtgtgcg caaatatggc agcgccaatg tgtggaagtt gctaaccgat ctttttgatt acttgccgct ggcagcggta gtggaaaacg agatcttttg ccttcacggt ggtctttctc ccacgttgga tagtctggcg catattcgta gcttagagcg cgtccaggag gttccccacg aggggcccat gtgcgatttg ttgtggtcgg atcctgaaga ccgtgatggg tggggcatca gtccacgtgg ggccggcttt acttttggcg ccgacatctc cgagcagttc tgtcacaaca acggcctcaa gatgattgca cgggcacatc agttggtgac ggacggatac agctgggcac ataatgatcg tctcgtgacg atatttagcg caccaaatta ctgctaccgt tgcggtaatc ttgccggatt gctggagctc gacgagcaca tgaataaatg tttttttcag tttgatcccg ccccgcgacg aggtgaggcg caggtgtcga agaaaacccc tgactacttt ctctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat