TrcrA.01169.b

protein phosphatase 2A catalytic subunit, putative

CENTER ID: TrcrA.01169.b
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.01169.b.B1.GE35138 full length 1 306
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053511211.60
RefSeq: XP_817727.1
UniProt: Q4DTP0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAELNEMIET LYAGKLISEQ QVAQLCDRCK DLFLEEGNIE VVYAPVTLCG DIHGQLFDML ELFRHGGRAP DTSYVFMGDF VDRGYHSVET LLLLLLLKVR YPDRMTLLRG NHESRQITQV YGFYDECYRK YGSANVWRFC TDLFDYMPLL ALVEEDVLCV HGGLSPDINT LDEIRVLDRK QEVPHEGPMT DLLWSDPDDI NGWALSPRGA GFIFGADVTK AFNHRNGITL IARAHQLVFE GYKSMFDDNC CTVWSAPNYC YRCGNVASIM KIGEKSRTDR SFITFGAAPA EERGHLTKKP PPEYFL
NT Sequence
atggcggagt tgaatgagat gatagagacg ttgtacgccg gcaaacttat tagtgagcag caggtggcgc agctgtgtga ccgctgcaag gacctctttc tcgaggaggg aaacattgag gttgtgtacg ctcccgtcac actgtgtggc gatatccacg gccagctatt tgacatgttg gagctctttc gtcatggcgg ccgcgcgccg gacaccagct atgtgttcat gggggacttt gtggaccgcg gctaccacag cgtcgagacc ctgttgctgc tgcttctact aaaagtccgc tacccagacc gcatgacact tttgcgcggt aatcacgagt cccgtcagat cacgcaggtg tatggctttt acgacgaatg ctacagaaag tacggtagcg ccaatgtctg gcgcttttgc accgatttgt ttgactacat gccgttattg gctttggtag aggaggacgt gttgtgcgtg cacggcggtc tgagcccaga cattaacaca ctcgatgaaa ttcgtgtttt ggatcgtaag caggaagttc cacacgaggg ccccatgaca gatcttctgt ggtccgaccc tgacgacatc aacggttggg cccttagccc acgaggtgcg ggatttattt ttggcgcaga cgtcacgaag gcatttaatc acaggaacgg catcacgctt attgcacgcg ctcaccaact cgtttttgaa ggctacaagt ccatgtttga cgacaactgt tgcacagtgt ggtccgctcc aaactactgc taccgctgcg gcaacgttgc gtccatcatg aaaataggcg agaagtcacg aacagaccgc agcttcatca catttggggc ggctcccgct gaagagcgcg ggcacttgac taaaaagccc ccgcccgagt acttcttgtg a
Details for TrcrA.01169.b.B1.GE35138
HARVESTED ON: 4/10/2012
SEQUENCED ON: 4/11/2012
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 98
PERCENT COVERAGE: 96
Validated AA Sequence
MAHHHHHHMA ELNEMIETLY AGKLISEQQV AQLCDRCKDL FLEEGNIEVV YAPVTLCGDI HGQLFDMLEL FRHGGRAPDT SYVFMGDFVD RGYHSVETLL LLLLLKVRYP DRMTLLRGNH ESRQITQVYG FYDECYRKYG SANVWRFCTD LFDYMPLLAL VEEDVLCVHG GLSPDINTLD EIRVLDRKQE VPHEGPMTDL LWSDPDDING WALSPRGAGF IFGADVTKAF NHRNGITLIA RAHQLVFEGY KSMFDDXCCT VWSAPNYCYR CGNVASIMKI GEKSRTDRSF ITFGAAPAXS AGT
Validated NT Sequence
cnnnnnnnnt tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgg agttgaatga gatgatagag acgttgtacg ccggcaaact tattagtgag cagcaggtgg cgcagctgtg tgaccgctgc aaggaccttt ttctcgagga gggaaacatt gaggttgtgt acgcccccgt cacactgtgt ggcgatatcc acggccagct atttgacatg ttggagctct ttcgtcatgg cggccgcgcg ccggacacca gctatgtgtt catgggggac tttgtggacc gtggctacca cagcgtcgag accctgttgc tgctgcttct actaaaagtc cgctacccag accgcatgac acttttgcgc ggtaatcacg agtcccgtca gattacgcag gtgtatggct tttacgacga atgctacaga aaatacggta gcgccaatgt ctggcgcttt tgcaccgatt tgtttgacta catgccgtta ttggctttgg tagaggagga cgtgttgtgc gtgcacggcg gtctgagccc agacattaac acactcgatg aaattcgtgt tttggatcgt aagcaggaag ttccacacga gggccccatg acagatcttc tgtggtccga ccctgacgac attaacggtt gggcccttag cccacgaggt gcgggattta tttttggcgc agacgtcacg aaggcattta atcacaggaa cggcatcacg cttatagcac gcgctcacca actcgttttt gaaggctaca aatccatgtt tgacgacanc tgttgcacag tgtggtccgc cccaaactac tgctaccgct gcggcaacgt tgcgtccatc atgaaaatag gcgagaagtc acgaacagac cgcagcttca tcacatttgg ggcggctccn gctganagcg cgggcacntg actaaaagcc ccgncngant acttcnngna agtgaganan nnnancnnnn nnnnnnnnnc c
Expected Protein Sequence
MAHHHHHHMA ELNEMIETLY AGKLISEQQV AQLCDRCKDL FLEEGNIEVV YAPVTLCGDI HGQLFDMLEL FRHGGRAPDT SYVFMGDFVD RGYHSVETLL LLLLLKVRYP DRMTLLRGNH ESRQITQVYG FYDECYRKYG SANVWRFCTD LFDYMPLLAL VEEDVLCVHG GLSPDINTLD EIRVLDRKQE VPHEGPMTDL LWSDPDDING WALSPRGAGF IFGADVTKAF NHRNGITLIA RAHQLVFEGY KSMFDDNCCT VWSAPNYCYR CGNVASIMKI GEKSRTDRSF ITFGAAPAEE RGHLTKKPPP EYFL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgg agttgaatga gatgatagag acgttgtacg ccggcaaact tattagtgag cagcaggtgg cgcagctgtg tgaccgctgc aaggacctct ttctcgagga gggaaacatt gaggttgtgt acgctcccgt cacactgtgt ggcgatatcc acggccagct atttgacatg ttggagctct ttcgtcatgg cggccgcgcg ccggacacca gctatgtgtt catgggggac tttgtggacc gcggctacca cagcgtcgag accctgttgc tgctgcttct actaaaagtc cgctacccag accgcatgac acttttgcgc ggtaatcacg agtcccgtca gatcacgcag gtgtatggct tttacgacga atgctacaga aagtacggta gcgccaatgt ctggcgcttt tgcaccgatt tgtttgacta catgccgtta ttggctttgg tagaggagga cgtgttgtgc gtgcacggcg gtctgagccc agacattaac acactcgatg aaattcgtgt tttggatcgt aagcaggaag ttccacacga gggccccatg acagatcttc tgtggtccga ccctgacgac atcaacggtt gggcccttag cccacgaggt gcgggattta tttttggcgc agacgtcacg aaggcattta atcacaggaa cggcatcacg cttattgcac gcgctcacca actcgttttt gaaggctaca agtccatgtt tgacgacaac tgttgcacag tgtggtccgc tccaaactac tgctaccgct gcggcaacgt tgcgtccatc atgaaaatag gcgagaagtc acgaacagac cgcagcttca tcacatttgg ggcggctccc gctgaagagc gcgggcactt gactaaaaag cccccgcccg agtacttctt gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat