TrcrA.01169.a

serine/threonine protein phosphatase, putative

CENTER ID: TrcrA.01169.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.01169.a.B1.GE35137 full length 1 340
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053509453.50
RefSeq: XP_810369.1
UniProt: Q4D7L6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEGALPTGIT NKTIASPTTT GTAVAVPTPP GAVGEPNSFN VDELIQYVLQ GKPLSEPQVA KLCQKGKEVL EKEGNVHTVR APVTVCGDIH GQFHDLLELL KIGGLPPDTN YLFMGDYVDR GYYSVETVTL LLLFKIRYPE RVQILRGNHE SRQITQVYGF YDECVRKYGS ANVWKLLTDL FDYLPLAAVV ENEIFCLHGG LSPTLDSLAH IRSLERVQEV PHEGPMCDLL WSDPEDRDGW GISPRGAGFT FGADISEQFC HNNGLKMIAR AHQLVTDGYS WAHNDRLVTI FSAPNYCYRC GNLAGLLELD EHMNKCFFQF DPAPRRGEAQ VSKKTPDYFL
NT Sequence
atggaggggg cgttacctac tggaattaca aataaaacga tagcgtcacc cacaaccaca gggacggcag tggcggtccc cacaccaccg ggtgcggtgg gtgagcctaa cagtttcaat gtggatgaat tgattcagta tgttttacag ggaaaaccat tgagtgagcc acaggtggcg aagctgtgcc agaaaggcaa ggaggttctg gagaaggagg ggaatgttca taccgtgcgt gcacccgtga ccgtatgtgg agatatacat ggtcaatttc atgatctgct ggagctgctg aagattggtg gtctcccacc tgacacgaat tatttattta tgggcgacta cgttgaccgc ggttactaca gtgtggagac agtcacattg ttgttgcttt ttaaaatacg atacccggag cgggttcaga ttcttcgtgg taaccatgaa tcacgccaga ttacgcaggt ctacggcttt tacgatgagt gtgtacgcaa atatggcagc gccaatgtgt ggaagttgct aaccgatctt tttgattact tgccgctggc ggcggtagtg gaaaacgaga tcttttgcct tcacggcggt ctttctccca cgttggatag tctggcgcat attcgtagct tagagcgcgt ccaggaggtt ccacacgagg ggcccatgtg cgatttgttg tggtcggatc ctgaggaccg tgatgggtgg ggcatcagtc cacgtggggc cggctttact tttggcgccg acatctccga gcagttttgt cacaacaacg gcctcaagat gattgcacgg gcacatcagt tggtgacgga cggatacagc tgggcacata atgatcgtct cgtgacgata tttagcgcac caaattactg ctaccgttgc ggtaaccttg ccggattgct ggagctcgac gagcacatga ataaatgttt ttttcagttt gatcccgccc cgcgacgagg tgaggcgcag gtgtcgaaga aaacccctga ctactttctc tga
Details for TrcrA.01169.a.B1.GE35137
HARVESTED ON: 4/10/2012
SEQUENCED ON: 4/11/2012
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 96
PERCENT COVERAGE: 92
Validated AA Sequence
MAHHHHHHME GALPTGITNK TIASPTTTGT AVAVPTPPGA VGEPNSFNVD ELIQYVLQGK PLSEPQVAKL CQKGKEVLEK EGNVHTVRAP VTVCGDIHGQ FHDLLELLKI GGLPPDTNYL FMGDYVDRGY YSVETVTLLL LFKIRYPERV QILRGNHESR QITQVYGFYD ECVRKYGSAN VWKLLTDLFD YLPLAAVVEN EIFCLHGGLS PTLDSLAHIR SLERVQEVPH EGPMCDLLWS DPEDRDGWGI SPRGAGFTFG ADXSEQFCHN NXLKMIARAH QLVTDGYXWA HNDRLVTIFS APNYCYRCGN XAXLLXARXA HE
Validated NT Sequence
ttttgtttnc tttaagaagg agatatacca tggctcacca ccaccaccac catatggagg gggcgttacc tactggaatt acaaataaaa cgatagcgtc acccacaacc acagggacgg cagtggcggt ccccacacca ccgggtgcgg tgggtgagcc taacagtttc aatgtggatg aattgattca gtatgtttta cagggaaaac cattgagtga gccacaggtg gcgaagctgt gccagaaagg caaggaggtt ctggagaagg aggggaatgt tcataccgtg cgtgcacccg tgaccgtatg tggagatata catggtcaat ttcatgatct gctggagctg ctgaagattg gtggtctccc acctgacacg aattatttat ttatgggcga ctacgttgac cgcggttact acagtgtgga gacagtcaca ttgttgttgc tttttaaaat acgatacccg gagcgggttc agattcttcg tggtaaccat gaatcacgcc agattacgca ggtctacggc ttttacgatg agtgtgtacg caaatatggc agcgccaatg tgtggaagtt gctaaccgat ctttttgatt acttgccgct ggcggcggta gtggaaaacg agatcttttg ccttcacggc ggtctttctc ccacgttgga tagtctggcg catattcgta gcttagagcg cgtccaggag gttccacacg aggggcccat gtgcgatttg ttgtggtcgg atcctgagga ccgtgatggg tggggcatca gtccacgtgg ggccggcttt acttttggcg ccgacntctc cgagcagttt tgtcacaaca acngcctcaa gatgattgca cgggcacatc agttggtgac ggacggatac ngctgggcac ataatgatcg tctcgtgacg atatttagcg caccaaatta ctgctaccgt tgcggtaacc ntgcngnatt gctngnagct cgacnagcac atgaataaat gttttttcag tttgatcccg ccccncgacn aggtnnnnnn cnnnntcnaa naaaacccct gactantttn tcnnnnnngn annnngnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa aaa
Expected Protein Sequence
MAHHHHHHME GALPTGITNK TIASPTTTGT AVAVPTPPGA VGEPNSFNVD ELIQYVLQGK PLSEPQVAKL CQKGKEVLEK EGNVHTVRAP VTVCGDIHGQ FHDLLELLKI GGLPPDTNYL FMGDYVDRGY YSVETVTLLL LFKIRYPERV QILRGNHESR QITQVYGFYD ECVRKYGSAN VWKLLTDLFD YLPLAAVVEN EIFCLHGGLS PTLDSLAHIR SLERVQEVPH EGPMCDLLWS DPEDRDGWGI SPRGAGFTFG ADISEQFCHN NGLKMIARAH QLVTDGYSWA HNDRLVTIFS APNYCYRCGN LAGLLELDEH MNKCFFQFDP APRRGEAQVS KKTPDYFL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggagg gggcgttacc tactggaatt acaaataaaa cgatagcgtc acccacaacc acagggacgg cagtggcggt ccccacacca ccgggtgcgg tgggtgagcc taacagtttc aatgtggatg aattgattca gtatgtttta cagggaaaac cattgagtga gccacaggtg gcgaagctgt gccagaaagg caaggaggtt ctggagaagg aggggaatgt tcataccgtg cgtgcacccg tgaccgtatg tggagatata catggtcaat ttcatgatct gctggagctg ctgaagattg gtggtctccc acctgacacg aattatttat ttatgggcga ctacgttgac cgcggttact acagtgtgga gacagtcaca ttgttgttgc tttttaaaat acgatacccg gagcgggttc agattcttcg tggtaaccat gaatcacgcc agattacgca ggtctacggc ttttacgatg agtgtgtacg caaatatggc agcgccaatg tgtggaagtt gctaaccgat ctttttgatt acttgccgct ggcggcggta gtggaaaacg agatcttttg ccttcacggc ggtctttctc ccacgttgga tagtctggcg catattcgta gcttagagcg cgtccaggag gttccacacg aggggcccat gtgcgatttg ttgtggtcgg atcctgagga ccgtgatggg tggggcatca gtccacgtgg ggccggcttt acttttggcg ccgacatctc cgagcagttt tgtcacaaca acggcctcaa gatgattgca cgggcacatc agttggtgac ggacggatac agctgggcac ataatgatcg tctcgtgacg atatttagcg caccaaatta ctgctaccgt tgcggtaacc ttgccggatt gctggagctc gacgagcaca tgaataaatg tttttttcag tttgatcccg ccccgcgacg aggtgaggcg caggtgtcga agaaaacccc tgactacttt ctctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat