TrcrA.01049.b
aspartate carbamoyltransferase, putative
| CENTER ID: | TrcrA.01049.b |
| ORGANISM: | Trypanosoma cruzi |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| TrcrA.01049.b.B1.GE35136 | full length | 1 | 326 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| TrcrA.01049.b.B1.PS01506 | full length | 1 | 326 | ||
| TrcrA.01049.b.B1.PS01511 | full length | 1 | 326 |
Structures
4IV5
DEPOSITED: 1/22/2013
DETERMINATION: XRay
CLONE: TrcrA.01049.b.B1.GE35136
PROTEIN: TrcrA.01049.b.B1.PS01511
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | TritrypDB:Tc00.1047053508375.30 |
| OrthoMCL: | OG5_128535 |
| RefSeq: | XP_809063.1 |
| UniProt: | O15636 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLELPPVASL KGKSITSAEQ FSRADIYALI HLASAMQRKI DAGEVLNLLQ GRIMTPLFFE DSSRTFSSFC AAMIRLGGSV VNFKVEASSI NKGETLADTI RTLDSYSDVL VMRHPRQDAI EEALSVAQHP ILNAGNGAGE HPTQALLDTL TIHSELGSVD GITIALIGDL KMGRTVHSLL KLLVRNFSIK CVFLVAPDAL QMPQDVLEPL QHEIATKGVI IHRTHALTDE VMQKSDVLYT TRLQKERFMA STSDDAAALQ SFAAKADITI DAARMRLAKE KMIVMHPLPR NDELSTTVDA DPRAAYFRQM RYGMFMRMAI LWSVLA
NT Sequence
atgttggaac tgccgccagt agcgagcctg aagggcaaaa gcatcacaag tgccgagcag ttcagtcgtg cggatatcta cgcgctgatt catcttgcaa gcgccatgca gcgtaagatt gatgcgggtg aggtgctgaa tctcctgcag ggccgcatca tgacgccact tttttttgag gattcctctc gcaccttcag ttccttttgt gccgcgatga ttcgacttgg tgggagcgtg gtgaacttta aggtcgaggc gtcatccatc aacaagggcg agaccctcgc cgacaccatt cgcacactgg actcctacag cgacgtgctg gtgatgcggc atccaaggca ggacgcgatt gaggaggccc tttccgtggc gcagcatccc attctgaatg cgggtaatgg ggctggcgaa cacccaacgc aggcgcttct ggacacgctg acgattcatt cggaattggg cagcgtagac ggcatcacca tcgcgctgat cggagacctt aagatggggc gaacggtgca ctctcttctg aagcttcttg tacgcaactt ttcgattaaa tgtgtgtttc ttgtcgcccc ggacgcactg cagatgccgc aggatgtgct ggaacccctg caacacgaga tcgccacgaa gggagtgata attcaccgca cacacgcact gacagatgag gtcatgcaaa agagtgatgt gttgtacaca acgcgtctgc agaaggaacg gtttatggct tccacgagcg atgatgcggc ggcgttgcag tcatttgcgg cgaaagccga catcaccatc gacgcggcac gtatgaggct ggcgaaggaa aaaatgatcg ttatgcaccc acttccacgc aatgacgagt taagcactac cgttgatgca gacccacgtg ctgcctactt taggcaaatg cggtacggca tgtttatgcg catggccatt ttgtggagcg ttttggcgtg a
Details for TrcrA.01049.b.B1.GE35136
| HARVESTED ON: | 4/10/2012 |
| SEQUENCED ON: | 4/11/2012 |
| EXPECTED MW: | 36kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 69 |
Validated AA Sequence
MQRKIDAGEV LNLLQGRIMT PLFFEDSSRT FSSFCAAMIR LGGSVVNFKV EASSINKGET LADTIRTLDS YSDVLVMRHP RQDAIEEALS VAQHPILNAG NGAGEHPTQA LLDTLTIHSE LGSVDGITIA LIGDLKMGRT VHSLLKLLVR NFSIKCVFLV APDALQMPQD VLEPLQHEIA TKGVIIHRTH ALTDEVMQKS DVLYTTRLQX ERFMASTSDD AAALQSFAAK A
Validated NT Sequence
gtcggctttc gccgcaaatg actgcaacgc cgccgcatca tcgctcgtgg aagccataaa ccgttcnttc tgcagacgcg ttgtgtacaa cacatcactc ttttgcatga cctcatctgt cagtgcgtgt gtgcggtgaa ttatcactcc cttcgtggcg atctcgtgtt gcaggggttc cagcacatcc tgcggcatct gcagtgcgtc cggggcgaca agaaacacac atttaatcga aaagttgcgt acaagaagct tcagaagaga gtgcaccgtt cgccccatct taaggtctcc gatcagcgcg atggtgatgc cgtctacgct gcccaattcc gaatgaatcg tcagcgtgtc cagaagcgcc tgcgttgggt gttcgccagc cccattaccc gcattcagaa tgggatgctg cgccacggaa agggcctcct caatcgcgtc ctgccttgga tgccgcatca ccagcacgtc gctgtaggag tccagtgtgc gaatggtgtc ggcgagggtc tcgcccttgt tgatggatga cgcctcgacc ttaaagttca ccacgctccc accaagtcga atcatcgcgg cacaaaagga actgaaggtg cgagaggaat cctcaaaaaa aagtggcgtc atgatgcggc cctgcaggag attcagcacc tcacccgcat caatcttacg ctgcatggcg cttgcaagat gaatcagcgc gtagatatcc gcacgactga actgctcggc acttgtgatg cttttgccct tcaggctcgc tactggcggc agttc
Expected Protein Sequence
MAHHHHHHML ELPPVASLKG KSITSAEQFS RADIYALIHL ASAMQRKIDA GEVLNLLQGR IMTPLFFEDS SRTFSSFCAA MIRLGGSVVN FKVEASSINK GETLADTIRT LDSYSDVLVM RHPRQDAIEE ALSVAQHPIL NAGNGAGEHP TQALLDTLTI HSELGSVDGI TIALIGDLKM GRTVHSLLKL LVRNFSIKCV FLVAPDALQM PQDVLEPLQH EIATKGVIIH RTHALTDEVM QKSDVLYTTR LQKERFMAST SDDAAALQSF AAKADITIDA ARMRLAKEKM IVMHPLPRND ELSTTVDADP RAAYFRQMRY GMFMRMAILW SVLA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttgg aactgccgcc agtagcgagc ctgaagggca aaagcatcac aagtgccgag cagttcagtc gtgcggatat ctacgcgctg attcatcttg caagcgccat gcagcgtaag attgatgcgg gtgaggtgct gaatctcctg cagggccgca tcatgacgcc actttttttt gaggattcct ctcgcacctt cagttccttt tgtgccgcga tgattcgact tggtgggagc gtggtgaact ttaaggtcga ggcgtcatcc atcaacaagg gcgagaccct cgccgacacc attcgcacac tggactccta cagcgacgtg ctggtgatgc ggcatccaag gcaggacgcg attgaggagg ccctttccgt ggcgcagcat cccattctga atgcgggtaa tggggctggc gaacacccaa cgcaggcgct tctggacacg ctgacgattc attcggaatt gggcagcgta gacggcatca ccatcgcgct gatcggagac cttaagatgg ggcgaacggt gcactctctt ctgaagcttc ttgtacgcaa cttttcgatt aaatgtgtgt ttcttgtcgc cccggacgca ctgcagatgc cgcaggatgt gctggaaccc ctgcaacacg agatcgccac gaagggagtg ataattcacc gcacacacgc actgacagat gaggtcatgc aaaagagtga tgtgttgtac acaacgcgtc tgcagaagga acggtttatg gcttccacga gcgatgatgc ggcggcgttg cagtcatttg cggcgaaagc cgacatcacc atcgacgcgg cacgtatgag gctggcgaag gaaaaaatga tcgttatgca cccacttcca cgcaatgacg agttaagcac taccgttgat gcagacccac gtgctgccta ctttaggcaa atgcggtacg gcatgtttat gcgcatggcc attttgtgga gcgttttggc gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for TrcrA.01049.b.B1.PS01506
| PURIFICATION DATe: | 5/24/2012 |
| CONCENTRATION: | 78.8mg/ml |
| OBSERVED MW: | 32kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 69 |
Protocol Notes
notes unavailable
Validated AA Sequence
MQRKIDAGEV LNLLQGRIMT PLFFEDSSRT FSSFCAAMIR LGGSVVNFKV EASSINKGET LADTIRTLDS YSDVLVMRHP RQDAIEEALS VAQHPILNAG NGAGEHPTQA LLDTLTIHSE LGSVDGITIA LIGDLKMGRT VHSLLKLLVR NFSIKCVFLV APDALQMPQD VLEPLQHEIA TKGVIIHRTH ALTDEVMQKS DVLYTTRLQX ERFMASTSDD AAALQSFAAK A
Validated NT Sequence
gtcggctttc gccgcaaatg actgcaacgc cgccgcatca tcgctcgtgg aagccataaa ccgttcnttc tgcagacgcg ttgtgtacaa cacatcactc ttttgcatga cctcatctgt cagtgcgtgt gtgcggtgaa ttatcactcc cttcgtggcg atctcgtgtt gcaggggttc cagcacatcc tgcggcatct gcagtgcgtc cggggcgaca agaaacacac atttaatcga aaagttgcgt acaagaagct tcagaagaga gtgcaccgtt cgccccatct taaggtctcc gatcagcgcg atggtgatgc cgtctacgct gcccaattcc gaatgaatcg tcagcgtgtc cagaagcgcc tgcgttgggt gttcgccagc cccattaccc gcattcagaa tgggatgctg cgccacggaa agggcctcct caatcgcgtc ctgccttgga tgccgcatca ccagcacgtc gctgtaggag tccagtgtgc gaatggtgtc ggcgagggtc tcgcccttgt tgatggatga cgcctcgacc ttaaagttca ccacgctccc accaagtcga atcatcgcgg cacaaaagga actgaaggtg cgagaggaat cctcaaaaaa aagtggcgtc atgatgcggc cctgcaggag attcagcacc tcacccgcat caatcttacg ctgcatggcg cttgcaagat gaatcagcgc gtagatatcc gcacgactga actgctcggc acttgtgatg cttttgccct tcaggctcgc tactggcggc agttc
Expressed Protein Sequence
MAHHHHHHML ELPPVASLKG KSITSAEQFS RADIYALIHL ASAMQRKIDA GEVLNLLQGR IMTPLFFEDS SRTFSSFCAA MIRLGGSVVN FKVEASSINK GETLADTIRT LDSYSDVLVM RHPRQDAIEE ALSVAQHPIL NAGNGAGEHP TQALLDTLTI HSELGSVDGI TIALIGDLKM GRTVHSLLKL LVRNFSIKCV FLVAPDALQM PQDVLEPLQH EIATKGVIIH RTHALTDEVM QKSDVLYTTR LQKERFMAST SDDAAALQSF AAKADITIDA ARMRLAKEKM IVMHPLPRND ELSTTVDADP RAAYFRQMRY GMFMRMAILW SVLA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTGG AACTGCCGCC AGTAGCGAGC CTGAAGGGCA AAAGCATCAC AAGTGCCGAG CAGTTCAGTC GTGCGGATAT CTACGCGCTG ATTCATCTTG CAAGCGCCAT GCAGCGTAAG ATTGATGCGG GTGAGGTGCT GAATCTCCTG CAGGGCCGCA TCATGACGCC ACTTTTTTTT GAGGATTCCT CTCGCACCTT CAGTTCCTTT TGTGCCGCGA TGATTCGACT TGGTGGGAGC GTGGTGAACT TTAAGGTCGA GGCGTCATCC ATCAACAAGG GCGAGACCCT CGCCGACACC ATTCGCACAC TGGACTCCTA CAGCGACGTG CTGGTGATGC GGCATCCAAG GCAGGACGCG ATTGAGGAGG CCCTTTCCGT GGCGCAGCAT CCCATTCTGA ATGCGGGTAA TGGGGCTGGC GAACACCCAA CGCAGGCGCT TCTGGACACG CTGACGATTC ATTCGGAATT GGGCAGCGTA GACGGCATCA CCATCGCGCT GATCGGAGAC CTTAAGATGG GGCGAACGGT GCACTCTCTT CTGAAGCTTC TTGTACGCAA CTTTTCGATT AAATGTGTGT TTCTTGTCGC CCCGGACGCA CTGCAGATGC CGCAGGATGT GCTGGAACCC CTGCAACACG AGATCGCCAC GAAGGGAGTG ATAATTCACC GCACACACGC ACTGACAGAT GAGGTCATGC AAAAGAGTGA TGTGTTGTAC ACAACGCGTC TGCAGAAGGA ACGGTTTATG GCTTCCACGA GCGATGATGC GGCGGCGTTG CAGTCATTTG CGGCGAAAGC CGACATCACC ATCGACGCGG CACGTATGAG GCTGGCGAAG GAAAAAATGA TCGTTATGCA CCCACTTCCA CGCAATGACG AGTTAAGCAC TACCGTTGAT GCAGACCCAC GTGCTGCCTA CTTTAGGCAA ATGCGGTACG GCATGTTTAT GCGCATGGCC ATTTTGTGGA GCGTTTTGGC GTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT
Details for TrcrA.01049.b.B1.PS01511
| PURIFICATION DATe: | 5/24/2012 |
| CONCENTRATION: | 92.37mg/ml |
| OBSERVED MW: | 32kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 69 |
Protocol Notes
notes unavailable
Validated AA Sequence
MQRKIDAGEV LNLLQGRIMT PLFFEDSSRT FSSFCAAMIR LGGSVVNFKV EASSINKGET LADTIRTLDS YSDVLVMRHP RQDAIEEALS VAQHPILNAG NGAGEHPTQA LLDTLTIHSE LGSVDGITIA LIGDLKMGRT VHSLLKLLVR NFSIKCVFLV APDALQMPQD VLEPLQHEIA TKGVIIHRTH ALTDEVMQKS DVLYTTRLQX ERFMASTSDD AAALQSFAAK A
Validated NT Sequence
gtcggctttc gccgcaaatg actgcaacgc cgccgcatca tcgctcgtgg aagccataaa ccgttcnttc tgcagacgcg ttgtgtacaa cacatcactc ttttgcatga cctcatctgt cagtgcgtgt gtgcggtgaa ttatcactcc cttcgtggcg atctcgtgtt gcaggggttc cagcacatcc tgcggcatct gcagtgcgtc cggggcgaca agaaacacac atttaatcga aaagttgcgt acaagaagct tcagaagaga gtgcaccgtt cgccccatct taaggtctcc gatcagcgcg atggtgatgc cgtctacgct gcccaattcc gaatgaatcg tcagcgtgtc cagaagcgcc tgcgttgggt gttcgccagc cccattaccc gcattcagaa tgggatgctg cgccacggaa agggcctcct caatcgcgtc ctgccttgga tgccgcatca ccagcacgtc gctgtaggag tccagtgtgc gaatggtgtc ggcgagggtc tcgcccttgt tgatggatga cgcctcgacc ttaaagttca ccacgctccc accaagtcga atcatcgcgg cacaaaagga actgaaggtg cgagaggaat cctcaaaaaa aagtggcgtc atgatgcggc cctgcaggag attcagcacc tcacccgcat caatcttacg ctgcatggcg cttgcaagat gaatcagcgc gtagatatcc gcacgactga actgctcggc acttgtgatg cttttgccct tcaggctcgc tactggcggc agttc
Expressed Protein Sequence
MAHHHHHHML ELPPVASLKG KSITSAEQFS RADIYALIHL ASAMQRKIDA GEVLNLLQGR IMTPLFFEDS SRTFSSFCAA MIRLGGSVVN FKVEASSINK GETLADTIRT LDSYSDVLVM RHPRQDAIEE ALSVAQHPIL NAGNGAGEHP TQALLDTLTI HSELGSVDGI TIALIGDLKM GRTVHSLLKL LVRNFSIKCV FLVAPDALQM PQDVLEPLQH EIATKGVIIH RTHALTDEVM QKSDVLYTTR LQKERFMAST SDDAAALQSF AAKADITIDA ARMRLAKEKM IVMHPLPRND ELSTTVDADP RAAYFRQMRY GMFMRMAILW SVLA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTGG AACTGCCGCC AGTAGCGAGC CTGAAGGGCA AAAGCATCAC AAGTGCCGAG CAGTTCAGTC GTGCGGATAT CTACGCGCTG ATTCATCTTG CAAGCGCCAT GCAGCGTAAG ATTGATGCGG GTGAGGTGCT GAATCTCCTG CAGGGCCGCA TCATGACGCC ACTTTTTTTT GAGGATTCCT CTCGCACCTT CAGTTCCTTT TGTGCCGCGA TGATTCGACT TGGTGGGAGC GTGGTGAACT TTAAGGTCGA GGCGTCATCC ATCAACAAGG GCGAGACCCT CGCCGACACC ATTCGCACAC TGGACTCCTA CAGCGACGTG CTGGTGATGC GGCATCCAAG GCAGGACGCG ATTGAGGAGG CCCTTTCCGT GGCGCAGCAT CCCATTCTGA ATGCGGGTAA TGGGGCTGGC GAACACCCAA CGCAGGCGCT TCTGGACACG CTGACGATTC ATTCGGAATT GGGCAGCGTA GACGGCATCA CCATCGCGCT GATCGGAGAC CTTAAGATGG GGCGAACGGT GCACTCTCTT CTGAAGCTTC TTGTACGCAA CTTTTCGATT AAATGTGTGT TTCTTGTCGC CCCGGACGCA CTGCAGATGC CGCAGGATGT GCTGGAACCC CTGCAACACG AGATCGCCAC GAAGGGAGTG ATAATTCACC GCACACACGC ACTGACAGAT GAGGTCATGC AAAAGAGTGA TGTGTTGTAC ACAACGCGTC TGCAGAAGGA ACGGTTTATG GCTTCCACGA GCGATGATGC GGCGGCGTTG CAGTCATTTG CGGCGAAAGC CGACATCACC ATCGACGCGG CACGTATGAG GCTGGCGAAG GAAAAAATGA TCGTTATGCA CCCACTTCCA CGCAATGACG AGTTAAGCAC TACCGTTGAT GCAGACCCAC GTGCTGCCTA CTTTAGGCAA ATGCGGTACG GCATGTTTAT GCGCATGGCC ATTTTGTGGA GCGTTTTGGC GTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT