TrcrA.01049.a

aspartate carbamoyltransferase, putative

CENTER ID: TrcrA.01049.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.01049.a.B1.GE35135 full length 1 327
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053511923.110
RefSeq: XP_813643.1
UniProt: Q4DGZ0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLEVPPVASL KGKSITSAEQ FSRADIYALI HLAGAMQRKI DAGEVLNLLQ GRIMTPLFFE DSSRTFSSFC AAMIRLGGSV VNFKFEASSI NKGETLADTI RTLDSYSDVL VMRHPRQDAI EEALSVAQHP ILNAGNGSGE HPTQALLDTL TIHSELGSVD GITIALIGDL KMGRTVHSLL KLLVRNFSIK CVFLVAPDAL QMPKDVLESL QHEIATKGVI IHRAHALTDE VMQTSDVLYT TRLQKERFMA STSDDAAALQ SFAAAKADIT IDAARMRLAK EKMIVMHPLP RNDELSTTVD TDPRAAYFRQ MRYGMFMRMA ILWSVLA
NT Sequence
atgttggaag tgccgccagt agcgagcctg aagggcaaaa gcatcacaag tgccgagcag ttcagtcgtg cggatatcta cgcgctgatt catcttgcag gtgccatgca gcgtaagatt gatgcgggag aggtgctgaa tctcctgcag ggccgcatca tgacgccact tttttttgag gattcctctc gcactttcag ttccttttgt gccgcgatga ttcgacttgg tgggagcgtg gtgaacttta agttcgaggc gtcatccatc aacaagggcg agaccctcgc cgacaccatt cgcacactgg actcttacag cgacgtgctg gtgatgcggc atccaaggca ggacgcgatt gaagaggccc tctccgtggc gcagcatccc attctgaatg cgggcaatgg gtctggcgaa cacccaacgc aggcgcttct ggacacgctg acgattcatt cggaattggg cagcgtagac ggcatcacca tcgcgctgat cggagacctg aagatggggc gaacggtgca ctctcttttg aagcttcttg tgcgcaactt ttctattaaa tgcgtgtttc ttgtcgcccc ggacgcactg cagatgccga aggatgtgct ggagtccctg caacacgaga tcgccacgaa gggagtgata attcaccgcg cacacgcact gacagatgag gtcatgcaga cgagtgatgt gttgtacaca acgcgtctgc agaaggaacg gtttatggct tccacgagcg atgatgcggc ggcgttgcag tcatttgcgg cggcgaaagc cgacatcacc atcgacgcgg cacgtatgag gctggcgaag gaaaaaatga ttgttatgca tccgctgccg cgcaatgacg agttaagcac taccgttgac acagacccac gtgctgccta ctttagacaa atgcggtacg gcatgtttat gcgcatggcc attttgtgga gcgttttggc gtga
Details for TrcrA.01049.a.B1.GE35135
HARVESTED ON: 4/10/2012
SEQUENCED ON: 4/11/2012
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML EVPPVASLKG KSITSAEQFS RADIYALIHL ASAMQRKIDA GEVLNLLQGR IMTPLFFEDS SRTFSSFCAA MIRLGGSVVN FKVEASSINK GETLADTIRT LDSYSDVLVM RHPRQDAIEE ALSVAQHPIL NAGNGAGEHP TQALLDTLTI HSELGSVDGI TIALIGDLKM GRTVHSLLKL LVRNFSIKCV FLVAPDALQM PQDVLEPLQH EIATKGVIIH RTHALTDEVM QKSDVLYTTR LQKERFMAST SDDAAALQSF AAKADITIDA ARMRLAKEKM IVMHPLPRND ELSTTVDADP RAAYFRQMRY GMFMRMAILW SVLA
Validated NT Sequence
atggctcacc accaccacca ccatatgttg gaagtgccgc cagtagcgag cctgaagggc aaaagcatca caagtgccga gcagttcagt cgtgcggata tctacgcgct gattcatctt gcaagcgcca tgcagcgtaa gattgatgcg ggtgaggtgc tgaatctcct gcagggccgc atcatgacgc cacttttttt tgaggattcc tctcgcacct tcagttcctt ttgtgccgcg atgattcgac ttggtgggag cgtggtgaac tttaaggtcg aggcgtcatc catcaacaag ggcgagaccc tcgccgacac cattcgcaca ctggactcct acagcgacgt gctggtgatg cggcatccaa ggcaggacgc gattgaggag gccctttccg tggcgcagca tcccattctg aatgcgggta atggggctgg cgaacaccca acgcaggcgc ttctggacac gctgacgatt cattcggaat tgggcagcgt agacggcatc accatcgcgc tgatcggaga ccttaagatg gggcgaacgg tgcactctct tctgaagctt cttgtacgca acttttcgat taaatgtgtg tttcttgtcg ccccggacgc actgcagatg ccgcaggatg tgctggaacc cctgcaacac gagatcgcca cgaagggagt gataattcac cgcacacacg cactgacaga tgaggtcatg caaaagagtg atgtgttgta cacaacgcgt ctgcagaagg aacggtttat ggcttccacg agcgatgatg cggcggcgtt gcagtcattt gcggcgaaag ccgacatcac catcgacgcg gcacgtatga ggctggcgaa ggaaaaaatg atcgttatgc acccacttcc acgcaatgac gagttaagca ctaccgttga tgcagaccca cgtgctgcct actttaggca aatgcggtac ggcatgttta tgcgcatggc cattttgtgg agcgttttgg cgtaa
Expected Protein Sequence
MAHHHHHHML EVPPVASLKG KSITSAEQFS RADIYALIHL AGAMQRKIDA GEVLNLLQGR IMTPLFFEDS SRTFSSFCAA MIRLGGSVVN FKFEASSINK GETLADTIRT LDSYSDVLVM RHPRQDAIEE ALSVAQHPIL NAGNGSGEHP TQALLDTLTI HSELGSVDGI TIALIGDLKM GRTVHSLLKL LVRNFSIKCV FLVAPDALQM PKDVLESLQH EIATKGVIIH RAHALTDEVM QTSDVLYTTR LQKERFMAST SDDAAALQSF AAAKADITID AARMRLAKEK MIVMHPLPRN DELSTTVDTD PRAAYFRQMR YGMFMRMAIL WSVLA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttgg aagtgccgcc agtagcgagc ctgaagggca aaagcatcac aagtgccgag cagttcagtc gtgcggatat ctacgcgctg attcatcttg caggtgccat gcagcgtaag attgatgcgg gagaggtgct gaatctcctg cagggccgca tcatgacgcc actttttttt gaggattcct ctcgcacttt cagttccttt tgtgccgcga tgattcgact tggtgggagc gtggtgaact ttaagttcga ggcgtcatcc atcaacaagg gcgagaccct cgccgacacc attcgcacac tggactctta cagcgacgtg ctggtgatgc ggcatccaag gcaggacgcg attgaagagg ccctctccgt ggcgcagcat cccattctga atgcgggcaa tgggtctggc gaacacccaa cgcaggcgct tctggacacg ctgacgattc attcggaatt gggcagcgta gacggcatca ccatcgcgct gatcggagac ctgaagatgg ggcgaacggt gcactctctt ttgaagcttc ttgtgcgcaa cttttctatt aaatgcgtgt ttcttgtcgc cccggacgca ctgcagatgc cgaaggatgt gctggagtcc ctgcaacacg agatcgccac gaagggagtg ataattcacc gcgcacacgc actgacagat gaggtcatgc agacgagtga tgtgttgtac acaacgcgtc tgcagaagga acggtttatg gcttccacga gcgatgatgc ggcggcgttg cagtcatttg cggcggcgaa agccgacatc accatcgacg cggcacgtat gaggctggcg aaggaaaaaa tgattgttat gcatccgctg ccgcgcaatg acgagttaag cactaccgtt gacacagacc cacgtgctgc ctactttaga caaatgcggt acggcatgtt tatgcgcatg gccattttgt ggagcgtttt ggcgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat