TrcrA.01024.a
enolase
| CENTER ID: | TrcrA.01024.a |
| ORGANISM: | Trypanosoma cruzi |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| TrcrA.01024.a.B1.GE35134 | full length | 1 | 429 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| TrcrA.01024.a.B1.PW35751 | full length | 1 | 429 |
Structures
4G7F
DEPOSITED: 7/20/2012
DETERMINATION: XRay
CLONE: TrcrA.01024.a.B1.GE35134
PROTEIN: TrcrA.01024.a.B1.PW35751
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | TritrypDB:Tc00.1047053504105.140 |
| OrthoMCL: | OG5_126698 |
| RefSeq: | XP_819700.1 |
| UniProt: | Q4DZ98 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTIQKVHGRE ILDSRGNPTV EVEVTTELGV FRSAVPSGAS TGIHEACELR DDDKRRYLGK GCLNAVKNVN DVLAPALVGK DELQQSTLDK LMRDLDGTPN KSKLGANAIL GCSMAISKAA AARKGVPLYR YLAELAGTKE VRLPVPCFNV INGGKHAGNA LPFQEFMIAP VKAGSFNEAL RMGAEVYHSL KSIIKKKYGQ DAVNVGDEGG FAPPITDINE PLPILMEAIE QAGHKGRFAI CMDSAASETY DENKKQYNLT FKSPEATWVT AKQLAETYAK WVSEYPIVSL EDPYDQDDFD GFAGITEALK GKAQVVGDDL TVTNVSRIKT AIEKKACNSL LLKINQIGTI TEAIEASKFC MSNGWSVMVS HRSGETEDTY IADLVVGLGT GQIKTGAPCR GERTAKLNQL LRIEEELGAH AKFGFPAWS
NT Sequence
atgacaatcc aaaaggttca cggacgcgag attcttgact cgcgtggaaa ccccactgtt gaggtagagg taacgacgga actgggcgtg ttccgctccg ccgttccgtc cggtgcgtcg accggcatcc acgaggcatg cgagctgcgc gatgatgaca agaggcgcta cctgggcaag ggctgcctca atgcggtgaa gaatgtgaac gatgtcctgg cgcccgcact tgtgggcaaa gacgagctgc agcagtcgac gctggacaag ctgatgcgtg accttgacgg gacaccaaac aagagcaaac ttggcgcaaa cgccattctc ggctgctcga tggcgatcag caaggcagcg gcggctcgca agggcgttcc gctgtaccgt tacctggcag agctggccgg cacaaaggag gttcgtcttc ccgtgccttg cttcaacgtc atcaacggtg gcaagcacgc gggcaacgcg ttgcccttcc aggaatttat gattgctcca gtgaaggcag gctctttcaa cgaggcgctg cgcatgggtg cggaggtgta tcactcgctg aagagcatca tcaagaagaa gtacggccag gatgcggtga acgtgggcga cgagggtggc tttgctcctc caatcacgga catcaatgag ccgctgccga ttctcatgga ggcgattgaa caggctggac acaagggcag gtttgctatc tgcatggact ccgctgccag tgagacatat gacgagaaca agaagcagta caacctgacc ttcaagagcc ctgaagcgac ctgggtgacg gcgaagcagc tggccgagac ttacgccaaa tgggtgagcg agtaccctat tgtgagcctc gaggaccctt acgaccagga cgactttgat ggctttgccg gcatcacgga ggctctcaag ggcaaggctc aggtcgtcgg tgacgactta actgtgacga atgtgtcacg catcaaaacg gccattgaga agaaggcatg caactcgctg ctgcttaaga tcaaccagat cggtacgatc acggaggcca tcgaggcatc caagttctgc atgtcgaacg gctggtcggt gatggtgtcg caccgcagtg gtgagacgga ggacacgtac attgctgacc ttgtggtggg cctcggcact ggccagatca agacgggcgc gccgtgccgt ggcgagcgca ccgcaaaact gaaccagcta ctccgcatcg aagaagagct tggtgcacac gccaagttcg gcttccccgc atggtcgtag
Details for TrcrA.01024.a.B1.GE35134
| HARVESTED ON: | 4/10/2012 |
| SEQUENCED ON: | 4/11/2012 |
| EXPECTED MW: | 47kDa |
| OBSERVED MW: | 47kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 75 |
Validated AA Sequence
MRDLDGTPNK SKLGANAILG CSMAISKAAA ARKGVPLYRY LAELAGTKEV RLPVPCFNVI NGGKHAGNAL PFQEFMIAPV KAGSFNEALR MGAEVYHSLK SIIKKKYGQD AVNVGDEGGF APPITDINEP LPILMEAIEQ AGHKGRFAIC MDSAASETYD ENKKQYNLTF KSPEATWVTA KQLAETYAKW VSEYPIVSLE DPYDQDDFDG FAGITETLKG KAQVVGDDLT VTNVSRIKMA IEKKACNSLL LKINQIGTIT EAIEASKFCM SNGWSVMVSH RSGETEDTYI ADLVVGLGTG QIKTGAPCRG ERTAKLNQLL RIEEELGAH
Validated NT Sequence
cgtgtgcacc aagctcttct tcgatgcgga gtagctggtt cagttttgcg gtgcgctcgc cacggcacgg cgcgcccgtc ttgatttggc cggtgccgag gcccaccaca aggtcagcaa tgtacgtgtc ctccgtctca ccactgcggt gcgacaccat caccgaccag ccgttcgaca tgcagaattt cgatgcctcg atggcctccg tgatcgtacc gatctggttg atcttaagca gcagcgagtt gcatgccttc ttctcaatgg ccattttgat gcgtgacaca ttcgtcacag ttaagtcgtc accgacgacc tgagccttgc ccttgagagt ctccgtgatg ccggcaaagc catcaaagtc gtcctggtcg taagggtcct cgaggcttac aatagggtac tcgctcaccc atttcgcgta agtctcggcc agctgcttcg ccgtcaccca ggtcgcttca gggctcttga aggtcaggtt gtattgcttc ttgttctcgt catatgtctc actggcagcg gagtccatgc agatggcaaa cctgcccttg tgtccagcct gttcaatcgc ctccataaga atcggcagcg gctcattgat gtccgtgatt ggaggagcaa agccaccctc gtcgcccacg ttcaccgcat cctggccgta cttcttcttg atgatgctct tcagcgagtg atacacctcc gcacccatgc gcagcgcttc attgaaagag cctgccttca ctggagcaat cataaattcc tggaagggca acgcgttgcc cgcgtgcttg ccaccgttga tgacgttgaa gcaaggcacg ggaagacgaa cctcctttgt gccggccagc tctgccaggt aacggtacag cggaacgccc ttgcgagccg ccgctgcctt gctgatcgcc atggagcagc cgagaatggc gtttgcgcca agtttgctct tgtttggtgt cccgtcaagg tcacgcatca gcttgtccag cgtcgactgc tgcagctcgt ctttgcccac aagtgcgggc gccaggacat cgttcacatt cttcaccgca ttgaggcagc ccttgcccag gtagcgcctc ttgtcatcat cgcgcagctc gcatgcctcg tggatgccgg tcgacgcacc ggacggaacg gcggagcgga acacgcccag ttccgtcgtt acctctacct caacagtggg gtttccacgc gagtcaagaa tctcgcgtcc gtgaaccttt tggattg
Expected Protein Sequence
MAHHHHHHMT IQKVHGREIL DSRGNPTVEV EVTTELGVFR SAVPSGASTG IHEACELRDD DKRRYLGKGC LNAVKNVNDV LAPALVGKDE LQQSTLDKLM RDLDGTPNKS KLGANAILGC SMAISKAAAA RKGVPLYRYL AELAGTKEVR LPVPCFNVIN GGKHAGNALP FQEFMIAPVK AGSFNEALRM GAEVYHSLKS IIKKKYGQDA VNVGDEGGFA PPITDINEPL PILMEAIEQA GHKGRFAICM DSAASETYDE NKKQYNLTFK SPEATWVTAK QLAETYAKWV SEYPIVSLED PYDQDDFDGF AGITEALKGK AQVVGDDLTV TNVSRIKTAI EKKACNSLLL KINQIGTITE AIEASKFCMS NGWSVMVSHR SGETEDTYIA DLVVGLGTGQ IKTGAPCRGE RTAKLNQLLR IEEELGAHAK FGFPAWS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacaa tccaaaaggt tcacggacgc gagattcttg actcgcgtgg aaaccccact gttgaggtag aggtaacgac ggaactgggc gtgttccgct ccgccgttcc gtccggtgcg tcgaccggca tccacgaggc atgcgagctg cgcgatgatg acaagaggcg ctacctgggc aagggctgcc tcaatgcggt gaagaatgtg aacgatgtcc tggcgcccgc acttgtgggc aaagacgagc tgcagcagtc gacgctggac aagctgatgc gtgaccttga cgggacacca aacaagagca aacttggcgc aaacgccatt ctcggctgct cgatggcgat cagcaaggca gcggcggctc gcaagggcgt tccgctgtac cgttacctgg cagagctggc cggcacaaag gaggttcgtc ttcccgtgcc ttgcttcaac gtcatcaacg gtggcaagca cgcgggcaac gcgttgccct tccaggaatt tatgattgct ccagtgaagg caggctcttt caacgaggcg ctgcgcatgg gtgcggaggt gtatcactcg ctgaagagca tcatcaagaa gaagtacggc caggatgcgg tgaacgtggg cgacgagggt ggctttgctc ctccaatcac ggacatcaat gagccgctgc cgattctcat ggaggcgatt gaacaggctg gacacaaggg caggtttgct atctgcatgg actccgctgc cagtgagaca tatgacgaga acaagaagca gtacaacctg accttcaaga gccctgaagc gacctgggtg acggcgaagc agctggccga gacttacgcc aaatgggtga gcgagtaccc tattgtgagc ctcgaggacc cttacgacca ggacgacttt gatggctttg ccggcatcac ggaggctctc aagggcaagg ctcaggtcgt cggtgacgac ttaactgtga cgaatgtgtc acgcatcaaa acggccattg agaagaaggc atgcaactcg ctgctgctta agatcaacca gatcggtacg atcacggagg ccatcgaggc atccaagttc tgcatgtcga acggctggtc ggtgatggtg tcgcaccgca gtggtgagac ggaggacacg tacattgctg accttgtggt gggcctcggc actggccaga tcaagacggg cgcgccgtgc cgtggcgagc gcaccgcaaa actgaaccag ctactccgca tcgaagaaga gcttggtgca cacgccaagt tcggcttccc cgcatggtcg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for TrcrA.01024.a.B1.PW35751
| PURIFICATION DATe: | 5/14/2012 |
| CONCENTRATION: | 47.62mg/ml |
| OBSERVED MW: | 45kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 75 |
Protocol Notes
notes unavailable
Validated AA Sequence
MRDLDGTPNK SKLGANAILG CSMAISKAAA ARKGVPLYRY LAELAGTKEV RLPVPCFNVI NGGKHAGNAL PFQEFMIAPV KAGSFNEALR MGAEVYHSLK SIIKKKYGQD AVNVGDEGGF APPITDINEP LPILMEAIEQ AGHKGRFAIC MDSAASETYD ENKKQYNLTF KSPEATWVTA KQLAETYAKW VSEYPIVSLE DPYDQDDFDG FAGITETLKG KAQVVGDDLT VTNVSRIKMA IEKKACNSLL LKINQIGTIT EAIEASKFCM SNGWSVMVSH RSGETEDTYI ADLVVGLGTG QIKTGAPCRG ERTAKLNQLL RIEEELGAH
Validated NT Sequence
cgtgtgcacc aagctcttct tcgatgcgga gtagctggtt cagttttgcg gtgcgctcgc cacggcacgg cgcgcccgtc ttgatttggc cggtgccgag gcccaccaca aggtcagcaa tgtacgtgtc ctccgtctca ccactgcggt gcgacaccat caccgaccag ccgttcgaca tgcagaattt cgatgcctcg atggcctccg tgatcgtacc gatctggttg atcttaagca gcagcgagtt gcatgccttc ttctcaatgg ccattttgat gcgtgacaca ttcgtcacag ttaagtcgtc accgacgacc tgagccttgc ccttgagagt ctccgtgatg ccggcaaagc catcaaagtc gtcctggtcg taagggtcct cgaggcttac aatagggtac tcgctcaccc atttcgcgta agtctcggcc agctgcttcg ccgtcaccca ggtcgcttca gggctcttga aggtcaggtt gtattgcttc ttgttctcgt catatgtctc actggcagcg gagtccatgc agatggcaaa cctgcccttg tgtccagcct gttcaatcgc ctccataaga atcggcagcg gctcattgat gtccgtgatt ggaggagcaa agccaccctc gtcgcccacg ttcaccgcat cctggccgta cttcttcttg atgatgctct tcagcgagtg atacacctcc gcacccatgc gcagcgcttc attgaaagag cctgccttca ctggagcaat cataaattcc tggaagggca acgcgttgcc cgcgtgcttg ccaccgttga tgacgttgaa gcaaggcacg ggaagacgaa cctcctttgt gccggccagc tctgccaggt aacggtacag cggaacgccc ttgcgagccg ccgctgcctt gctgatcgcc atggagcagc cgagaatggc gtttgcgcca agtttgctct tgtttggtgt cccgtcaagg tcacgcatca gcttgtccag cgtcgactgc tgcagctcgt ctttgcccac aagtgcgggc gccaggacat cgttcacatt cttcaccgca ttgaggcagc ccttgcccag gtagcgcctc ttgtcatcat cgcgcagctc gcatgcctcg tggatgccgg tcgacgcacc ggacggaacg gcggagcgga acacgcccag ttccgtcgtt acctctacct caacagtggg gtttccacgc gagtcaagaa tctcgcgtcc gtgaaccttt tggattg
Expressed Protein Sequence
MAHHHHHHMT IQKVHGREIL DSRGNPTVEV EVTTELGVFR SAVPSGASTG IHEACELRDD DKRRYLGKGC LNAVKNVNDV LAPALVGKDE LQQSTLDKLM RDLDGTPNKS KLGANAILGC SMAISKAAAA RKGVPLYRYL AELAGTKEVR LPVPCFNVIN GGKHAGNALP FQEFMIAPVK AGSFNEALRM GAEVYHSLKS IIKKKYGQDA VNVGDEGGFA PPITDINEPL PILMEAIEQA GHKGRFAICM DSAASETYDE NKKQYNLTFK SPEATWVTAK QLAETYAKWV SEYPIVSLED PYDQDDFDGF AGITEALKGK AQVVGDDLTV TNVSRIKTAI EKKACNSLLL KINQIGTITE AIEASKFCMS NGWSVMVSHR SGETEDTYIA DLVVGLGTGQ IKTGAPCRGE RTAKLNQLLR IEEELGAHAK FGFPAWS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACAA TCCAAAAGGT TCACGGACGC GAGATTCTTG ACTCGCGTGG AAACCCCACT GTTGAGGTAG AGGTAACGAC GGAACTGGGC GTGTTCCGCT CCGCCGTTCC GTCCGGTGCG TCGACCGGCA TCCACGAGGC ATGCGAGCTG CGCGATGATG ACAAGAGGCG CTACCTGGGC AAGGGCTGCC TCAATGCGGT GAAGAATGTG AACGATGTCC TGGCGCCCGC ACTTGTGGGC AAAGACGAGC TGCAGCAGTC GACGCTGGAC AAGCTGATGC GTGACCTTGA CGGGACACCA AACAAGAGCA AACTTGGCGC AAACGCCATT CTCGGCTGCT CGATGGCGAT CAGCAAGGCA GCGGCGGCTC GCAAGGGCGT TCCGCTGTAC CGTTACCTGG CAGAGCTGGC CGGCACAAAG GAGGTTCGTC TTCCCGTGCC TTGCTTCAAC GTCATCAACG GTGGCAAGCA CGCGGGCAAC GCGTTGCCCT TCCAGGAATT TATGATTGCT CCAGTGAAGG CAGGCTCTTT CAACGAGGCG CTGCGCATGG GTGCGGAGGT GTATCACTCG CTGAAGAGCA TCATCAAGAA GAAGTACGGC CAGGATGCGG TGAACGTGGG CGACGAGGGT GGCTTTGCTC CTCCAATCAC GGACATCAAT GAGCCGCTGC CGATTCTCAT GGAGGCGATT GAACAGGCTG GACACAAGGG CAGGTTTGCT ATCTGCATGG ACTCCGCTGC CAGTGAGACA TATGACGAGA ACAAGAAGCA GTACAACCTG ACCTTCAAGA GCCCTGAAGC GACCTGGGTG ACGGCGAAGC AGCTGGCCGA GACTTACGCC AAATGGGTGA GCGAGTACCC TATTGTGAGC CTCGAGGACC CTTACGACCA GGACGACTTT GATGGCTTTG CCGGCATCAC GGAGGCTCTC AAGGGCAAGG CTCAGGTCGT CGGTGACGAC TTAACTGTGA CGAATGTGTC ACGCATCAAA ACGGCCATTG AGAAGAAGGC ATGCAACTCG CTGCTGCTTA AGATCAACCA GATCGGTACG ATCACGGAGG CCATCGAGGC ATCCAAGTTC TGCATGTCGA ACGGCTGGTC GGTGATGGTG TCGCACCGCA GTGGTGAGAC GGAGGACACG TACATTGCTG ACCTTGTGGT GGGCCTCGGC ACTGGCCAGA TCAAGACGGG CGCGCCGTGC CGTGGCGAGC GCACCGCAAA ACTGAACCAG CTACTCCGCA TCGAAGAAGA GCTTGGTGCA CACGCCAAGT TCGGCTTCCC CGCATGGTCG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT