TrcrA.01003.a

cell division control protein 2 homolog 2,cdc2-related kinase

CENTER ID: TrcrA.01003.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.01003.a.B1.GE35133 full length 1 330
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053503617.10
RefSeq: XP_806767.1
UniProt: Q4CXB8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNPAAGGVVY DTLSSQPQRI MPQPHYAALE ANERYFRQEK VGEGSYGVVY KCLDRQTGRI VAVKRISLKL KDEGVPATAV REVSLLRELN HPYVVQLLDV SLQDSKLLLI FEYMEKDLQG LLKQRNTPLV GGKLQRIMFQ LLLGLHACHS RRFVHRDIKP SNILISRDES VVKLADFGLG RAFRVPLQTY TTEVMTLWYR APEVLLGDNH YLPAIDVWSM GCVMAELAKG TPLFAADTAI SQLFAIFQVL GTPSESTWRG VSSLSHHNVD FPQWRPTSLS SVIPTLEPEG IDLLQRMLLY DPRQRITAYD ALRHSWFDDV RHDMSEEAAL
NT Sequence
atgaatccag cagcaggggg tgtggtatac gacacgttgt cgtcacagcc acagaggatt atgccccaac cacactacgc cgcattggaa gccaatgagc gttactttcg tcaggagaaa gtaggtgaag ggtcatacgg tgttgtgtac aaatgccttg ataggcaaac aggtcgcatt gtcgctgtga aacggatctc gttgaagctt aaggatgaag gggtaccggc aacggcggta cgggaagtgt ctcttcttcg agaattgaat catccgtacg ttgtgcagct tcttgatgta tcccttcagg attcgaagtt gcttcttatc tttgaataca tggaaaagga tttacaggga ttattgaagc agcgaaacac accacttgtg ggaggaaaac tgcaacgtat tatgtttcag ttattgttag gattacacgc ctgtcacagc cgtcgcttcg tacatcgtga cattaagcca agcaatattc tcatcagccg cgatgagtcg gtggtgaaat tggcggattt tggtttgggt cgtgcctttc gtgttccgct gcaaacatac acgacggagg tgatgacact gtggtaccgt gcaccggagg tcctactcgg ggataatcat taccttcctg ccattgatgt gtggtcgatg ggatgtgtca tggcagaact tgcgaaagga acgcctctct ttgccgctga taccgccatc agccaattgt ttgccatatt tcaagtcctc ggcaccccgt cggagtctac atggcgtggt gtgtcctcct tgtcgcacca caatgtcgac ttccctcagt ggcggcctac ctcactgagt agtgtcatcc ccacactgga gccagaggga atcgacctgc tgcaacgcat gctgctctac gatccacgtc agcgtatcac cgcgtatgac gcgcttcgtc acagttggtt tgacgacgtg cggcatgaca tgagtgagga ggccgctctt taa
Details for TrcrA.01003.a.B1.GE35133
HARVESTED ON: 4/10/2012
SEQUENCED ON: 4/11/2012
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 91
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN PAAGGVVYDT LSSQPQRIMP QPHYAALEAN ERYFRQEKVG EGSYGVVYKC LDRQTGRIVA VKRISLKLKD EGVPATAVRE VSLLRELNHP YVVQLLDVSL QDSKLLLIFE YMEKDLQGLL KQRNTPLVGG KLQRIMFQLL LGLHACHSRR FVHRDIKPSN ILISRDESVV KLADFGLGRA FRVPLQTYTT XVMTLWYRAP EVLLGDNHYL PAIDVWSMGC VMAELAKGTP LFAADTAISQ LFAIFQVLGT PSXSTWRGVS SLSHHNVDFP QWRPTSLXSV IPTLXPEGID LLQRMLLYDP RQRIXXMTRF VTVGXTXXXM TXXXXXXX
Validated NT Sequence
tttntttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatc cagcagcagg gggtgtggta tacgacacgt tgtcgtcaca gccacagagg attatgcccc aaccacacta cgccgcattg gaagccaatg agcgttactt tcgtcaggag aaagtaggtg aagggtcata cggtgttgtg tacaaatgcc ttgataggca aacaggtcgc attgtcgctg tgaaacggat ctcgttgaag cttaaggatg aaggggtacc ggcaacggcg gtacgggaag tgtctcttct tcgagaattg aatcatccgt acgttgtgca gcttcttgat gtatcccttc aggattcgaa gttgcttctt atctttgaat acatggaaaa ggatttacag ggattattga agcagcgaaa cacaccactt gtgggaggaa aactgcaacg tattatgttt cagttattgt taggattaca cgcctgtcac agccgtcgct tcgtacatcg tgacattaag ccaagcaata ttctcatcag ccgcgatgag tcggtggtga aattggcgga ttttggtttg ggtcgtgcct ttcgtgttcc gctgcaaaca tacacgacgg angtgatgac actgtggtac cgtgcaccgg aggtcctact cggggataat cattaccttc ctgccattga tgtgtggtcg atgggatgtg tcatggcaga acttgcgaaa ggaacgcctc tctttgccgc tgataccgcc atcagccaat tgtttgccat atttcaagtc ctcggcaccc cgtcggantc tacatggcgt ggtgtgtcct ccttgtcgca ccacaatgtc gacttccctc agtggcggcc tacctcactg antagtgtca tccccacact ggnnccagag ggaatcgacc tgctgcaacg catgctgctc tacgatccac gtcagcgtat cnnnngtatg acgcgcttcg tcacagttgg nttnacnann tgnngcatga cntgnntgan ngnnnnnnnt nnntgagnan nnnnncnnnn nnnnnnnnnn a
Expected Protein Sequence
MAHHHHHHMN PAAGGVVYDT LSSQPQRIMP QPHYAALEAN ERYFRQEKVG EGSYGVVYKC LDRQTGRIVA VKRISLKLKD EGVPATAVRE VSLLRELNHP YVVQLLDVSL QDSKLLLIFE YMEKDLQGLL KQRNTPLVGG KLQRIMFQLL LGLHACHSRR FVHRDIKPSN ILISRDESVV KLADFGLGRA FRVPLQTYTT EVMTLWYRAP EVLLGDNHYL PAIDVWSMGC VMAELAKGTP LFAADTAISQ LFAIFQVLGT PSESTWRGVS SLSHHNVDFP QWRPTSLSSV IPTLEPEGID LLQRMLLYDP RQRITAYDAL RHSWFDDVRH DMSEEAAL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatc cagcagcagg gggtgtggta tacgacacgt tgtcgtcaca gccacagagg attatgcccc aaccacacta cgccgcattg gaagccaatg agcgttactt tcgtcaggag aaagtaggtg aagggtcata cggtgttgtg tacaaatgcc ttgataggca aacaggtcgc attgtcgctg tgaaacggat ctcgttgaag cttaaggatg aaggggtacc ggcaacggcg gtacgggaag tgtctcttct tcgagaattg aatcatccgt acgttgtgca gcttcttgat gtatcccttc aggattcgaa gttgcttctt atctttgaat acatggaaaa ggatttacag ggattattga agcagcgaaa cacaccactt gtgggaggaa aactgcaacg tattatgttt cagttattgt taggattaca cgcctgtcac agccgtcgct tcgtacatcg tgacattaag ccaagcaata ttctcatcag ccgcgatgag tcggtggtga aattggcgga ttttggtttg ggtcgtgcct ttcgtgttcc gctgcaaaca tacacgacgg aggtgatgac actgtggtac cgtgcaccgg aggtcctact cggggataat cattaccttc ctgccattga tgtgtggtcg atgggatgtg tcatggcaga acttgcgaaa ggaacgcctc tctttgccgc tgataccgcc atcagccaat tgtttgccat atttcaagtc ctcggcaccc cgtcggagtc tacatggcgt ggtgtgtcct ccttgtcgca ccacaatgtc gacttccctc agtggcggcc tacctcactg agtagtgtca tccccacact ggagccagag ggaatcgacc tgctgcaacg catgctgctc tacgatccac gtcagcgtat caccgcgtat gacgcgcttc gtcacagttg gtttgacgac gtgcggcatg acatgagtga ggaggccgct ctttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat