TrcrA.00914.b
glyceraldehyde 3-phosphate dehydrogenase, glycosomal
| CENTER ID: | TrcrA.00914.b |
| ORGANISM: | Trypanosoma cruzi |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| TrcrA.00914.b.B1.GE35125 | full length | 1 | 338 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| TrcrA.00914.b.B1.PS01822 | full length | 1 | 338 |
Structures
4LSM
DEPOSITED: 7/22/2013
DETERMINATION: XRay
CLONE: TrcrA.00914.b.B1.GE35125
PROTEIN: TrcrA.00914.b.B1.PS01822
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | TritrypDB:Tc00.1047053510105.230 |
| OrthoMCL: | OG5_126628 |
| RefSeq: | XP_819899.1 |
| UniProt: | Q4DZT1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTEGKKVTIK IGINGFGRIG RMVFRAAQHR NDIEIVGIND LLDADYMAYM LKYDSTHGRY GGMVEVREGA LVVNGKKIRV TSERDPANLK WNEVGAVVVV ESTGLFLTDE TARKHIQAGA KKVVMTGPPK DDTPMFVMGV NNTMYKGQEI VSNASCTTNC LAPLAKIIHE KFGIVEGLMT TVHATTATQK TVDGPSQKDW RGGRGAAQNI IPSATGAAKA VGKVIPALNG RLTGMAFRVP TPNVSVVDLT ARLERPATYE QICAAIKAAS EGELKGILGY TEDEVVSTDM NGVALTSVFD VKAGISLNDR LVKLISWYDN ETGYSHKVLD LVAYISAH
NT Sequence
atgactgaag ggaaaaaagt aaccatcaag attggcatca atggctttgg ccgcatcggc cgaatggtct tccgcgccgc gcaacaccgc aacgacattg agattgttgg cattaacgat ctgctggacg cggattacat ggcgtacatg ctgaagtatg actccacgca cggtcgctac ggcggcatgg tggaggtgcg ggaaggcgct ctcgtcgtca acggcaagaa gatccgcgtg acaagtgagc gcgatcccgc caatctcaag tggaatgagg tcggcgcggt ggtcgtggtc gagtcgacag gtctcttcct caccgatgag accgcccgca agcacattca ggccggcgca aagaaagtcg tcatgacagg cccgccaaag gacgacacgc ccatgtttgt catgggtgtg aacaacacaa tgtacaaggg acaggaaatc gtctccaatg cctcgtgcac aacgaactgc cttgcgccgc ttgcgaagat tattcatgag aaattcggaa tcgttgaggg tctcatgacg acagtgcatg ccaccacggc aacacaaaaa acagtggatg gtccgtcgca gaaagactgg cgcggtggtc gcggtgcggc gcagaacatt attccgtcag ccaccggagc agccaaggcg gttgggaaag ttattcccgc gctgaacggc aggctgaccg gcatggcgtt ccgtgtgccg actcccaacg tgtccgttgt cgacctcacc gcgcgtctgg agaggccggc gacatacgag cagatctgcg ccgccatcaa ggcagcctct gagggggagc tgaaaggcat tcttggctac acggaggacg aggttgtttc cactgacatg aatggcgtcg cgctgacgtc cgtctttgac gtcaaggccg gcatttccct caacgatcgc ctcgtgaagc tcatctcgtg gtacgacaac gagaccgggt actcccacaa agtgctggac ctcgttgcct acatctcagc acactga
Details for TrcrA.00914.b.B1.GE35125
| HARVESTED ON: | 4/10/2012 |
| SEQUENCED ON: | 4/11/2012 |
| EXPECTED MW: | 37kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMT EGKKVTIKIG INGFGRIGRM VFRAAQHRND IEIVGINDLL DADYMAYMLK YDSTHGRYGG MVEVREGALV VNGKKIRVTS ERDPANLKWN EVGAVVVVES TGLFLTDETA RKHIQAGAKK VVMTGPPKDD TPMFVMGVNN TMYKGQEIVS NASCTTNCLA PLAKIIHEKF GIVEGLMTTV HATTATQKTV DGPSQKDWRG GRGAAQNIIP SATGAAKAVG KVIPALNGRL TGMAFRVPTP NVSVVDLTAR LERPATYEQI CAAIKAASEG ELKGILGYTE DEVVSTDMNG VALTSVFDVK AGISLNDRFV KLISWYDNET GYSHKVLDLV AYISAH
Validated NT Sequence
tactcactta gtgtgctgag atgtaggcaa cgaggtccag cactttgtgg gagtacccgg tttcgttgtc gtaccacgag atgagcttca cgaagcgatc gttgagggaa atgccggcct tgacgtcaaa gacggacgtc agcgcgacgc cattcatgtc agtggaaaca acctcgtcct ccgtgtagcc aagaatgcct ttaagctccc cctcagaggc tgccttgatg gcggcgcaga tctgctcgta tgtcgccggc ctctccagac gcgcggtgag gtcgacaacg gacacgttgg gagtcggcac acggaacgcc atgccggtca gcctgccgtt cagcgcggga ataactttcc caaccgcctt ggctgctccg gtggctgacg gaataatgtt ctgcgccgca ccgcgaccac cgcgccagtc tttctgcgac ggaccatcca ctgttttttg tgttgccgtg gtggcatgca ctgtcgtcat gagaccctca acgattccga atttctcatg aataatcttc gcaagcggcg caaggcagtt cgttgtgcac gaggcattgg agacgatttc ctgtcccttg tacattgtgt tgttcacacc catgacaaac atgggcgtgt cgtcctttgg cgggcctgtc ataacgactt tctttgcgcc ggcctgaatg tgcttgcggg cggtctcatc ggtgaggaag agacctgtcg actcgaccac gaccaccgcg ccgacctcat tccacttaag attggcggga tcgcgctcac ttgtcacgcg gatcttcttg ccgttgacga cgagagcgcc ttcccgcacc tccaccatgc cgccgtagcg accgtgcgtg gagtcatact tcagcatgta cgccatgtaa tccgcgtcca gcagatcgtt aatgccaaca atctcaatgt cgttgcggtg ttgcgcggcg cggaagacca ttcggccgat gcggccaaat ccattgatgc caatcttgat ggttactttt ttcccttcag tcatatggtg gtggtggtgg tgagccatgg tatntctcct tcttaa
Expected Protein Sequence
MAHHHHHHMT EGKKVTIKIG INGFGRIGRM VFRAAQHRND IEIVGINDLL DADYMAYMLK YDSTHGRYGG MVEVREGALV VNGKKIRVTS ERDPANLKWN EVGAVVVVES TGLFLTDETA RKHIQAGAKK VVMTGPPKDD TPMFVMGVNN TMYKGQEIVS NASCTTNCLA PLAKIIHEKF GIVEGLMTTV HATTATQKTV DGPSQKDWRG GRGAAQNIIP SATGAAKAVG KVIPALNGRL TGMAFRVPTP NVSVVDLTAR LERPATYEQI CAAIKAASEG ELKGILGYTE DEVVSTDMNG VALTSVFDVK AGISLNDRLV KLISWYDNET GYSHKVLDLV AYISAH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactg aagggaaaaa agtaaccatc aagattggca tcaatggctt tggccgcatc ggccgaatgg tcttccgcgc cgcgcaacac cgcaacgaca ttgagattgt tggcattaac gatctgctgg acgcggatta catggcgtac atgctgaagt atgactccac gcacggtcgc tacggcggca tggtggaggt gcgggaaggc gctctcgtcg tcaacggcaa gaagatccgc gtgacaagtg agcgcgatcc cgccaatctc aagtggaatg aggtcggcgc ggtggtcgtg gtcgagtcga caggtctctt cctcaccgat gagaccgccc gcaagcacat tcaggccggc gcaaagaaag tcgtcatgac aggcccgcca aaggacgaca cgcccatgtt tgtcatgggt gtgaacaaca caatgtacaa gggacaggaa atcgtctcca atgcctcgtg cacaacgaac tgccttgcgc cgcttgcgaa gattattcat gagaaattcg gaatcgttga gggtctcatg acgacagtgc atgccaccac ggcaacacaa aaaacagtgg atggtccgtc gcagaaagac tggcgcggtg gtcgcggtgc ggcgcagaac attattccgt cagccaccgg agcagccaag gcggttggga aagttattcc cgcgctgaac ggcaggctga ccggcatggc gttccgtgtg ccgactccca acgtgtccgt tgtcgacctc accgcgcgtc tggagaggcc ggcgacatac gagcagatct gcgccgccat caaggcagcc tctgaggggg agctgaaagg cattcttggc tacacggagg acgaggttgt ttccactgac atgaatggcg tcgcgctgac gtccgtcttt gacgtcaagg ccggcatttc cctcaacgat cgcctcgtga agctcatctc gtggtacgac aacgagaccg ggtactccca caaagtgctg gacctcgttg cctacatctc agcacactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for TrcrA.00914.b.B1.PS01822
| PURIFICATION DATe: | 5/15/2013 |
| CONCENTRATION: | 29mg/ml |
| OBSERVED MW: | 35kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 12 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT EGKKVTIKIG INGFGRIGRM VFRAAQHRND IEIVGINDLL DADYMAYMLK YDSTHGRYGG MVEVREGALV VNGKKIRVTS ERDPANLKWN EVGAVVVVES TGLFLTDETA RKHIQAGAKK VVMTGPPKDD TPMFVMGVNN TMYKGQEIVS NASCTTNCLA PLAKIIHEKF GIVEGLMTTV HATTATQKTV DGPSQKDWRG GRGAAQNIIP SATGAAKAVG KVIPALNGRL TGMAFRVPTP NVSVVDLTAR LERPATYEQI CAAIKAASEG ELKGILGYTE DEVVSTDMNG VALTSVFDVK AGISLNDRFV KLISWYDNET GYSHKVLDLV AYISAH
Validated NT Sequence
tactcactta gtgtgctgag atgtaggcaa cgaggtccag cactttgtgg gagtacccgg tttcgttgtc gtaccacgag atgagcttca cgaagcgatc gttgagggaa atgccggcct tgacgtcaaa gacggacgtc agcgcgacgc cattcatgtc agtggaaaca acctcgtcct ccgtgtagcc aagaatgcct ttaagctccc cctcagaggc tgccttgatg gcggcgcaga tctgctcgta tgtcgccggc ctctccagac gcgcggtgag gtcgacaacg gacacgttgg gagtcggcac acggaacgcc atgccggtca gcctgccgtt cagcgcggga ataactttcc caaccgcctt ggctgctccg gtggctgacg gaataatgtt ctgcgccgca ccgcgaccac cgcgccagtc tttctgcgac ggaccatcca ctgttttttg tgttgccgtg gtggcatgca ctgtcgtcat gagaccctca acgattccga atttctcatg aataatcttc gcaagcggcg caaggcagtt cgttgtgcac gaggcattgg agacgatttc ctgtcccttg tacattgtgt tgttcacacc catgacaaac atgggcgtgt cgtcctttgg cgggcctgtc ataacgactt tctttgcgcc ggcctgaatg tgcttgcggg cggtctcatc ggtgaggaag agacctgtcg actcgaccac gaccaccgcg ccgacctcat tccacttaag attggcggga tcgcgctcac ttgtcacgcg gatcttcttg ccgttgacga cgagagcgcc ttcccgcacc tccaccatgc cgccgtagcg accgtgcgtg gagtcatact tcagcatgta cgccatgtaa tccgcgtcca gcagatcgtt aatgccaaca atctcaatgt cgttgcggtg ttgcgcggcg cggaagacca ttcggccgat gcggccaaat ccattgatgc caatcttgat ggttactttt ttcccttcag tcatatggtg gtggtggtgg tgagccatgg tatntctcct tcttaa
Expressed Protein Sequence
MAHHHHHHMT EGKKVTIKIG INGFGRIGRM VFRAAQHRND IEIVGINDLL DADYMAYMLK YDSTHGRYGG MVEVREGALV VNGKKIRVTS ERDPANLKWN EVGAVVVVES TGLFLTDETA RKHIQAGAKK VVMTGPPKDD TPMFVMGVNN TMYKGQEIVS NASCTTNCLA PLAKIIHEKF GIVEGLMTTV HATTATQKTV DGPSQKDWRG GRGAAQNIIP SATGAAKAVG KVIPALNGRL TGMAFRVPTP NVSVVDLTAR LERPATYEQI CAAIKAASEG ELKGILGYTE DEVVSTDMNG VALTSVFDVK AGISLNDRLV KLISWYDNET GYSHKVLDLV AYISAH
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACTG AAGGGAAAAA AGTAACCATC AAGATTGGCA TCAATGGCTT TGGCCGCATC GGCCGAATGG TCTTCCGCGC CGCGCAACAC CGCAACGACA TTGAGATTGT TGGCATTAAC GATCTGCTGG ACGCGGATTA CATGGCGTAC ATGCTGAAGT ATGACTCCAC GCACGGTCGC TACGGCGGCA TGGTGGAGGT GCGGGAAGGC GCTCTCGTCG TCAACGGCAA GAAGATCCGC GTGACAAGTG AGCGCGATCC CGCCAATCTC AAGTGGAATG AGGTCGGCGC GGTGGTCGTG GTCGAGTCGA CAGGTCTCTT CCTCACCGAT GAGACCGCCC GCAAGCACAT TCAGGCCGGC GCAAAGAAAG TCGTCATGAC AGGCCCGCCA AAGGACGACA CGCCCATGTT TGTCATGGGT GTGAACAACA CAATGTACAA GGGACAGGAA ATCGTCTCCA ATGCCTCGTG CACAACGAAC TGCCTTGCGC CGCTTGCGAA GATTATTCAT GAGAAATTCG GAATCGTTGA GGGTCTCATG ACGACAGTGC ATGCCACCAC GGCAACACAA AAAACAGTGG ATGGTCCGTC GCAGAAAGAC TGGCGCGGTG GTCGCGGTGC GGCGCAGAAC ATTATTCCGT CAGCCACCGG AGCAGCCAAG GCGGTTGGGA AAGTTATTCC CGCGCTGAAC GGCAGGCTGA CCGGCATGGC GTTCCGTGTG CCGACTCCCA ACGTGTCCGT TGTCGACCTC ACCGCGCGTC TGGAGAGGCC GGCGACATAC GAGCAGATCT GCGCCGCCAT CAAGGCAGCC TCTGAGGGGG AGCTGAAAGG CATTCTTGGC TACACGGAGG ACGAGGTTGT TTCCACTGAC ATGAATGGCG TCGCGCTGAC GTCCGTCTTT GACGTCAAGG CCGGCATTTC CCTCAACGAT CGCCTCGTGA AGCTCATCTC GTGGTACGAC AACGAGACCG GGTACTCCCA CAAAGTGCTG GACCTCGTTG CCTACATCTC AGCACACTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT