TrcrA.00914.a

glyceraldehyde 3-phosphate dehydrogenase, glycosomal

CENTER ID:	TrcrA.00914.a
ORGANISM:	Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS:	crystal structure
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.00914.a.B1.GE35124	full length		1	338

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.00914.a.B1.PS01542	full length		1	338

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tc00.1047053509717.80
OrthoMCL:	OG5_126628
RefSeq:	XP_815568.1
UniProt:	Q4DMG2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTEGKKLTIK IGINGFGRIG RMVFRAAQHR NDIEIVGIND LLDADYMAYM LKYDSTHGRY GGMVEVREGA LVVNGKKIRV TSEHDPANLK WNEVGAVVVV ESTGLFLTDE TARKHIQAGA KKVVMTGPPK DDTPMFVMGV NNTMYKGQEI VSNASCTTNC LAPLAKIIHE KFGIVEGLMT TVHATTATQK TVGGPSQKDW RGGRGAAQNI VPSATGAAKA VGKVIPALNG RLTGMAFRVP TPNVSVVDLT ARLERPATYE QICAAIKAAS EGELKGILGY TEDEVVSTDM NGVALTSVFD VKAGISLNDH FAKLAPWYDN ETGYSHKVLD LVAYISAH

NT Sequence

atgactgaag ggaaaaaatt gaccatcaag attggcatca atggctttgg ccgcatcggc cgaatggtct tccgcgccgc gcaacaccgc aacgacattg agattgttgg cattaacgat ctgctggacg cggattacat ggcgtacatg ctgaagtatg actccacgca cggtcgctac ggcggcatgg tggaggtgcg ggaaggtgct cttgtcgtca acggcaagaa gatccgcgtg acaagtgagc acgatcccgc caatctcaag tggaatgagg tcggcgcggt ggtcgtggtc gagtcgacag gtctcttcct caccgatgag accgcccgca agcacattca ggccggcgca aagaaggtcg tcatgacagg cccgccaaag gacgacacgc ccatgtttgt catgggtgtg aacaacacga tgtacaaggg acaggaaatc gtctccaacg cctcgtgcac aacgaactgc cttgcgccgc ttgcgaagat tattcatgag aaatttggca tcgttgaggg tctcatgacg acagtgcatg ccaccacggc aacacaaaaa acagtgggtg gtccatcgca gaaagactgg cgcggaggtc gcggtgcggc gcagaacatc gttccgtccg ccaccggagc agccaaggcg gttgggaaag ttattcccgc gctgaacggc aggctgaccg gcatggcgtt ccgtgtgccg actccaaacg tgtctgttgt cgacctcacc gcgcgcctgg agaggccagc gacatacgag cagatctgcg ccgccatcaa ggcagcctct gagggggagc tgaaaggcat tcttggctac acggaggacg aggttgtttc cactgacatg aatggcgtcg cgctgacgtc cgtctttgac gtcaaggccg gtatttccct caacgatcac ttcgcgaagc tcgccccatg gtacgacaac gagactgggt actcccacaa agtgctggac ctcgttgcct acatctcagc acactga

Details for TrcrA.00914.a.B1.GE35124

HARVESTED ON:	4/10/2012
SEQUENCED ON:	4/11/2012
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	97

Validated AA Sequence

MTEGKKVTIK IGINGFGRIG RMVFRAAQHR NDIEIVGIND LLDADYMAYM LKYDSTHGRY GGMVEVREGA LVVNGKKIRV TSERDPANLK WNEVGAVVVV ESTGLFLTDE TARKHIQAGA KKVVMTGPPK DDTPMFVMGV NNTMYKGQEI VSNASCTTNC LAPLAKIIHE KFGIVEGLMT TVHATTATQK TVDGPSQKDW RGGRGAAQNI IPSATGAAKA VGKVIPALNG RLTGMAFRVP TPNVSVVDLT ARLERPATYE QICAAIKAAS EGELKGILGY TEDEVVSTDM NGVALTSVFD VKAGISLNDH FAKLAPWYDN ETGYSHKVLD LVAYISAH

Validated NT Sequence

tcttactcac ttagtgtgct gagatgtagg caacgaggtc cagcactttg tgggagtacc cagtctcgtt gtcgtaccat ggggcgagct tcgcgaagtg atcgttgagg gaaataccgg ccttgacgtc aaagacggac gtcagcgcga cgccattcat gtcagtggaa acaacctcgt cctccgtgta gccaagaatg cctttcagct ccccctcaga ggctgccttg atggcggcgc agatctgctc gtatgtcgct ggcctctcca ggcgcgcggt gaggtcgaca acagacacgt ttggagtcgg cacacggaac gccatgccgg tcagcctgcc gttcagcgcg ggaataacct tcccaaccgc cttggctgct ccggtggcgg acggaatgat gttctgcgcc gcaccgcgac ctccgcgcca gtctttctgc gacggaccat ccactgtttt ttgtgttgcc gtggtggcat gcactgtcgt catgagaccc tcaacgatgc caaatttctc atgaataatc ttcgcaagcg gcgcaaggca gttcgttgtg cacgaggcgt tggagacgat ttcctgtccc ttgtacattg tgttgttcac acccatgaca aacatgggcg tgtcgtcctt tggcgggcct gtcatgacga ctttctttgc gccggcctga atgtgcttgc gggcggtctc atcggtgagg aagagacctg tcgactcgac cacgaccacc gcgccgacct cattccactt gagattggcg ggatcgcgct cacttgtcac gcggatcttc ttgccgttga cgacgagagc gccttcccgc acctccacca tgccgccgta gcgaccgtgc gtggagtcat acttcagcat gtacgccatg taatccgcgt ccagcagatc gttaatgcca acaatctcaa tgtcgttgcg gtgttgcgcg gcgcggaaga ccattcggcc gatgcggcca aagccattga tgccaatctt gatggttact tttttccctt cagtcatatg gtggtggtgg tg

Expected Protein Sequence

MAHHHHHHMT EGKKLTIKIG INGFGRIGRM VFRAAQHRND IEIVGINDLL DADYMAYMLK YDSTHGRYGG MVEVREGALV VNGKKIRVTS EHDPANLKWN EVGAVVVVES TGLFLTDETA RKHIQAGAKK VVMTGPPKDD TPMFVMGVNN TMYKGQEIVS NASCTTNCLA PLAKIIHEKF GIVEGLMTTV HATTATQKTV GGPSQKDWRG GRGAAQNIVP SATGAAKAVG KVIPALNGRL TGMAFRVPTP NVSVVDLTAR LERPATYEQI CAAIKAASEG ELKGILGYTE DEVVSTDMNG VALTSVFDVK AGISLNDHFA KLAPWYDNET GYSHKVLDLV AYISAH

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactg aagggaaaaa attgaccatc aagattggca tcaatggctt tggccgcatc ggccgaatgg tcttccgcgc cgcgcaacac cgcaacgaca ttgagattgt tggcattaac gatctgctgg acgcggatta catggcgtac atgctgaagt atgactccac gcacggtcgc tacggcggca tggtggaggt gcgggaaggt gctcttgtcg tcaacggcaa gaagatccgc gtgacaagtg agcacgatcc cgccaatctc aagtggaatg aggtcggcgc ggtggtcgtg gtcgagtcga caggtctctt cctcaccgat gagaccgccc gcaagcacat tcaggccggc gcaaagaagg tcgtcatgac aggcccgcca aaggacgaca cgcccatgtt tgtcatgggt gtgaacaaca cgatgtacaa gggacaggaa atcgtctcca acgcctcgtg cacaacgaac tgccttgcgc cgcttgcgaa gattattcat gagaaatttg gcatcgttga gggtctcatg acgacagtgc atgccaccac ggcaacacaa aaaacagtgg gtggtccatc gcagaaagac tggcgcggag gtcgcggtgc ggcgcagaac atcgttccgt ccgccaccgg agcagccaag gcggttggga aagttattcc cgcgctgaac ggcaggctga ccggcatggc gttccgtgtg ccgactccaa acgtgtctgt tgtcgacctc accgcgcgcc tggagaggcc agcgacatac gagcagatct gcgccgccat caaggcagcc tctgaggggg agctgaaagg cattcttggc tacacggagg acgaggttgt ttccactgac atgaatggcg tcgcgctgac gtccgtcttt gacgtcaagg ccggtatttc cctcaacgat cacttcgcga agctcgcccc atggtacgac aacgagactg ggtactccca caaagtgctg gacctcgttg cctacatctc agcacactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for TrcrA.00914.a.B1.PS01542

PURIFICATION DATe:	6/28/2012
CONCENTRATION:	27.1mg/ml
OBSERVED MW:	34kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	110µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACTG AAGGGAAAAA ATTGACCATC AAGATTGGCA TCAATGGCTT TGGCCGCATC GGCCGAATGG TCTTCCGCGC CGCGCAACAC CGCAACGACA TTGAGATTGT TGGCATTAAC GATCTGCTGG ACGCGGATTA CATGGCGTAC ATGCTGAAGT ATGACTCCAC GCACGGTCGC TACGGCGGCA TGGTGGAGGT GCGGGAAGGT GCTCTTGTCG TCAACGGCAA GAAGATCCGC GTGACAAGTG AGCACGATCC CGCCAATCTC AAGTGGAATG AGGTCGGCGC GGTGGTCGTG GTCGAGTCGA CAGGTCTCTT CCTCACCGAT GAGACCGCCC GCAAGCACAT TCAGGCCGGC GCAAAGAAGG TCGTCATGAC AGGCCCGCCA AAGGACGACA CGCCCATGTT TGTCATGGGT GTGAACAACA CGATGTACAA GGGACAGGAA ATCGTCTCCA ACGCCTCGTG CACAACGAAC TGCCTTGCGC CGCTTGCGAA GATTATTCAT GAGAAATTTG GCATCGTTGA GGGTCTCATG ACGACAGTGC ATGCCACCAC GGCAACACAA AAAACAGTGG GTGGTCCATC GCAGAAAGAC TGGCGCGGAG GTCGCGGTGC GGCGCAGAAC ATCGTTCCGT CCGCCACCGG AGCAGCCAAG GCGGTTGGGA AAGTTATTCC CGCGCTGAAC GGCAGGCTGA CCGGCATGGC GTTCCGTGTG CCGACTCCAA ACGTGTCTGT TGTCGACCTC ACCGCGCGCC TGGAGAGGCC AGCGACATAC GAGCAGATCT GCGCCGCCAT CAAGGCAGCC TCTGAGGGGG AGCTGAAAGG CATTCTTGGC TACACGGAGG ACGAGGTTGT TTCCACTGAC ATGAATGGCG TCGCGCTGAC GTCCGTCTTT GACGTCAAGG CCGGTATTTC CCTCAACGAT CACTTCGCGA AGCTCGCCCC ATGGTACGAC AACGAGACTG GGTACTCCCA CAAAGTGCTG GACCTCGTTG CCTACATCTC AGCACACTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT