SSGCID: Target Page

TrcrA.00795.b

hexokinase, putative

CENTER ID:	TrcrA.00795.b
ORGANISM:	Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.00795.b.B1.GE35123	full length		1	471

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tc00.1047053510121.20
RefSeq:	XP_816463.1
UniProt:	Q4DQ27

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSARLNNLLQ HIAVKDKDSD TMRHLKQRMA LASLANQFTV GKDHLKQLML YMVHEMIEGL EGRESTLRML PSYVYKTDPS KATGVFYALD LGGTNFRVLR VTCKEGRVAD RVDAKFVIPQ QALQGTAEDL FGFIAQSVKK MMEQKAPEDL NRTVPLGFTF SFPTEQKGVD HGFLIKWTKG FSTRGVEGKD VVELLQKALK RMEVKVKVVA LCNDTVGTLI TNYFFDPDTQ VGVIIGTGSN ACYFEDAYAV TKEPSVAARG TTQTPINMEC GNFDSKYKFV LPVTAYDEAM DAVTPNRNYQ TQEKMVSGMY LGEISRRMIA HLAELHCLPS ALASKMAKPW SFETKFMGMI SADRMPGLQF TRQVFQELFH VDVTDVADLH VIRDVCCLVR GRAAQISAMF CSAPLVKTRK EGRATVAIDG SVFEKTPSFR RLLQQNMNAI LGPGCDVTTA LARDGSGIGA AFISALVVND K

NT Sequence

atgtccgctc gcctgaacaa cctgctgcag cacattgctg tgaaagacaa agacagcgac acaatgcgac acctgaagca gcgcatggcg ctggcgtccc tcgcgaacca gttcacggtg ggcaaggacc acctcaagca gctgatgttg tacatggtcc acgagatgat tgagggactg gagggccgcg agagcacttt gcgcatgctg ccttcttacg tgtacaaaac cgatcccagc aaggcgacag gcgtcttcta cgcgctggac ctcgggggca cgaactttcg agtgctgcgt gtgacgtgca aggaaggaag ggtagcggac cgtgtggacg ccaagtttgt gattccgcag caggcactgc aaggaacggc agaggatttg tttggcttta ttgcgcagag tgtgaagaaa atgatggagc agaaagcacc ggaggacttg aaccgcaccg tgccgcttgg cttcacattt agctttccaa cagagcagaa aggcgtcgat cacggtttcc tgataaaatg gacgaagggt ttctcgacac ggggtgtgga ggggaaggac gtggtggaac ttctgcagaa ggcattgaaa cgcatggaag tgaaggtgaa agtggtggca ctctgcaacg acactgtcgg tacgcttatc acgaactact tctttgatcc cgatacgcag gttggcgtaa tcatcggcac cggctccaac gcgtgctact tcgaggatgc ctacgccgtg acgaaggaac cctcagtggc cgcgcgcggt acgacacaga caccaatcaa catggagtgc gggaactttg actccaagta caagtttgtg ctgccggtca ccgcgtacga cgaggcgatg gatgccgtta cgccgaaccg caactaccag acgcaagaga agatggtctc cggcatgtat cttggggaaa tcagtcgccg catgatagcg catcttgccg agttgcattg cctgccaagc gcccttgcat ccaagatggc caagccgtgg agctttgaga cgaaatttat gggcatgata tccgcagacc gcatgccggg tctgcagttc acgcgtcaag tgttccaaga gcttttccat gtcgacgtga cggatgttgc agacctgcac gtgatccgtg atgtgtgctg cctggtgcgc ggccgcgctg cgcagatcag tgccatgttc tgcagtgcgc cacttgtgaa gacaaggaaa gagggccgtg cgacggtcgc gatcgatggc tccgtgtttg agaagacacc gtcattccgc cgcctgctgc agcaaaacat gaacgccatt ctcggtcctg ggtgtgatgt cacgacggca ctcgcaaggg acggcagcgg cattggcgcc gcttttatct ccgcgctggt tgtgaacgac aaataa

Details for TrcrA.00795.b.B1.GE35123

HARVESTED ON:	4/10/2012
SEQUENCED ON:	4/11/2012
EXPECTED MW:	53kDa
OBSERVED MW:	53kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	92
PERCENT COVERAGE:	67

Validated AA Sequence

Validated NT Sequence

anattttgtn nnntttaaga aggaganata ccatggctca ccaccaccac caccatatgt ccgctcgcct gaacaacctg ctgcagcaca ttgctgtgaa agacaaagac agcgacacaa tgcgacacct gaagcagcgc atggcgctgg cgtccctcgc gaaccagttc acggtgggca aggaccacct caagcagctg atgttgtaca tggtccacga gatgattgag ggactggagg gccgcgagag cactttgcgc atgctgcctt cttacgtgta caaaaccgat cccagcaagg cgacaggcgt cttctacgcg ctggacctcg ggggcacgaa ctttcgagtg ctgcgtgtga cgtgcaagga aggaagggta gcggaccgtg tggacgccaa gtttgtgatt ccgcagcagg cactgcaagg aacggcagag gatttgtttg gctttattgc gcagagtgtg aagaaaatga tggagcagaa agcaccggag gacttgaacc gcaccgtgcc gcttggcttc acatttagct ttccaacaga gcagaaaggc gtcgatcacg gtttcctgat aaaatggacg aagggtttct cgacacgggg tgtggagggg aaggacgtgg tggaacttct gcagaaggca ttgaaacgca tggaagtgaa ggtgaaagtg gtggcactct gcaacgacac tgtcggtacg cttatcacga actacttctt tgatcccgat acgcangttg gcgtaatcat cggcaccggc tccnacgcgt gctacttcga ggatgcctac gccgtgacga annaaccctc agtggccgcg cgcggtacga cacagacacc aatcaacatg gagtgcggga actttgactc cnagtacaag tttgtgctgc cggtcancgc gtacgacgag gcgatggatg ccgtacnccn aanngcaact accagacgca aganaanang gtctncngna tgtatcntgg ggaaatcagt cnnnncatga tancgcatcn ngncnannnc attgcctnnn ncgncnnnna tnnnnngnnn ncnnnnnctt nnnacnannn ntggnntgan nncnnnnacn nannnnnnnn gcnntnnnnn nncannnnnn cnnnnnnnnn nncnnannnn nnncnnnnnn nnnnnnnnnn nnnncnnnnn naaannnnnn ncc

Expected Protein Sequence

MAHHHHHHMS ARLNNLLQHI AVKDKDSDTM RHLKQRMALA SLANQFTVGK DHLKQLMLYM VHEMIEGLEG RESTLRMLPS YVYKTDPSKA TGVFYALDLG GTNFRVLRVT CKEGRVADRV DAKFVIPQQA LQGTAEDLFG FIAQSVKKMM EQKAPEDLNR TVPLGFTFSF PTEQKGVDHG FLIKWTKGFS TRGVEGKDVV ELLQKALKRM EVKVKVVALC NDTVGTLITN YFFDPDTQVG VIIGTGSNAC YFEDAYAVTK EPSVAARGTT QTPINMECGN FDSKYKFVLP VTAYDEAMDA VTPNRNYQTQ EKMVSGMYLG EISRRMIAHL AELHCLPSAL ASKMAKPWSF ETKFMGMISA DRMPGLQFTR QVFQELFHVD VTDVADLHVI RDVCCLVRGR AAQISAMFCS APLVKTRKEG RATVAIDGSV FEKTPSFRRL LQQNMNAILG PGCDVTTALA RDGSGIGAAF ISALVVNDK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg ctcgcctgaa caacctgctg cagcacattg ctgtgaaaga caaagacagc gacacaatgc gacacctgaa gcagcgcatg gcgctggcgt ccctcgcgaa ccagttcacg gtgggcaagg accacctcaa gcagctgatg ttgtacatgg tccacgagat gattgaggga ctggagggcc gcgagagcac tttgcgcatg ctgccttctt acgtgtacaa aaccgatccc agcaaggcga caggcgtctt ctacgcgctg gacctcgggg gcacgaactt tcgagtgctg cgtgtgacgt gcaaggaagg aagggtagcg gaccgtgtgg acgccaagtt tgtgattccg cagcaggcac tgcaaggaac ggcagaggat ttgtttggct ttattgcgca gagtgtgaag aaaatgatgg agcagaaagc accggaggac ttgaaccgca ccgtgccgct tggcttcaca tttagctttc caacagagca gaaaggcgtc gatcacggtt tcctgataaa atggacgaag ggtttctcga cacggggtgt ggaggggaag gacgtggtgg aacttctgca gaaggcattg aaacgcatgg aagtgaaggt gaaagtggtg gcactctgca acgacactgt cggtacgctt atcacgaact acttctttga tcccgatacg caggttggcg taatcatcgg caccggctcc aacgcgtgct acttcgagga tgcctacgcc gtgacgaagg aaccctcagt ggccgcgcgc ggtacgacac agacaccaat caacatggag tgcgggaact ttgactccaa gtacaagttt gtgctgccgg tcaccgcgta cgacgaggcg atggatgccg ttacgccgaa ccgcaactac cagacgcaag agaagatggt ctccggcatg tatcttgggg aaatcagtcg ccgcatgata gcgcatcttg ccgagttgca ttgcctgcca agcgcccttg catccaagat ggccaagccg tggagctttg agacgaaatt tatgggcatg atatccgcag accgcatgcc gggtctgcag ttcacgcgtc aagtgttcca agagcttttc catgtcgacg tgacggatgt tgcagacctg cacgtgatcc gtgatgtgtg ctgcctggtg cgcggccgcg ctgcgcagat cagtgccatg ttctgcagtg cgccacttgt gaagacaagg aaagagggcc gtgcgacggt cgcgatcgat ggctccgtgt ttgagaagac accgtcattc cgccgcctgc tgcagcaaaa catgaacgcc attctcggtc ctgggtgtga tgtcacgacg gcactcgcaa gggacggcag cggcattggc gccgctttta tctccgcgct ggttgtgaac gacaaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t