TrcrA.00766.b

protein kinase, putative,cdc2-related related kinase, putative

CENTER ID:	TrcrA.00766.b
ORGANISM:	Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.00766.b.B1.GE35121	full length		1	294

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tc00.1047053503423.20
OrthoMCL:	OG5_133587
RefSeq:	XP_804705.1
UniProt:	Q4CRF3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDRFIVGSPI GEGRFGVVRA AVVKESGVPA AIKCITTPRL DEGIPHPTAR ELLVAMRLVH PFVIRTYEVV PCGCSLALVT ERCKTDLATV LSGRTASNPL PALEAKSLFK MLLLALAYIH SEGVLHRDVK PSNCFLTAEG ELRLGDFGLS RVWDTEASMT HEVVSRWYRA PELLLGQRHY GPEIDVWSSG CVFAELLRGY GGAFFAGDGD IWQLSRILDV LGTPTPTSWP SAVLLPDWGK LHFEPKRPLP LREFFPDASE CALDLLRRLL CLDPQKRLTA AGALCHAYFS EYPT

NT Sequence

atggatcgtt ttattgttgg atccccaatt ggtgagggcc gctttggcgt cgttcgtgct gcagttgtta aggaatctgg agtgccagcc gcaatcaaat gcatcacaac tccacgtctc gatgagggta ttccgcaccc cacagcgagg gaactgctcg ttgcaatgcg attggtgcat ccttttgtta ttcggacata tgaggtagtt ccgtgtggct gctccttggc gttagtgacg gagcgttgca agactgactt ggcaacagtc ctctcgggga gaactgccag caatcctctg cccgcgttag aggcaaagtc tttatttaaa atgcttcttt tggcgttagc gtacatacac agcgagggtg ttcttcatcg agatgtgaaa ccttcaaatt gtttcttgac tgcggagggg gaactacgcc tcggcgactt tggacttagt cgtgtatggg atacggaagc atccatgact cacgaagtcg tttcgaggtg gtaccgggct cccgagttac ttctgggcca gcgacactac ggacccgaaa ttgatgtgtg gtcatcaggg tgcgtatttg cggaacttct acgagggtac ggtggtgctt tttttgcagg cgatggagat atttggcagt tgtcacgaat attagatgta ttagggacac cgacgcccac atcgtggcca tcggcggtgc tgcttcccga ttggggaaag ttgcactttg agccgaagcg tcctcttcct ctccgtgaat ttttcccgga tgcctctgaa tgtgcattgg atttgttgcg aagattactt tgcctggatc ctcagaagcg tttgactgcg gctggcgccc tttgtcacgc ctactttagt gaatacccga cgtga

Details for TrcrA.00766.b.B1.GE35121

HARVESTED ON:	data unavailable
SEQUENCED ON:	4/11/2012
EXPECTED MW:	33kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	96
PERCENT COVERAGE:	72

Validated AA Sequence

MAHHHHHHMD RFIVGSPIGE GRFGVVRAAV VKESGVPAAI KCITTPRLDE GIPHPTAREL LVALRLVHPF VIRTYEVAPC GCSLALVTER CKTDLATVLS GRTASNPLPA LEAKTLFKML LLALAYIHSE GVLHRDVKPS NCFLTAEGEL RLGDFGLSRV WDTEASMTHE VVSRWYRAPE LLLGQRHYGP EIDVWSSGCV FAELLRGYGG AFFCRRWRY

Validated NT Sequence

tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatc gttttattgt tggatcccca attggtgagg gccgctttgg cgtcgttcgt gctgcagttg ttaaggaatc tggagtgcca gccgcaatca aatgcatcac aactccacgt ctcgatgagg gtattccgca ccccacagcg agggaactac tcgttgcatt gcggttggtg cacccttttg ttattcggac atatgaggta gctccatgcg gctgctcctt ggcgttagtg acggagcgtt gcaagactga cttggcaaca gtcctctcgg ggagaactgc cagcaatcct ctgcccgcgt tagaggcaaa gactttattt aagatgcttc ttttggcgtt agcgtacata cacagcgaag gtgttcttca tcgagatgtg aaaccttcaa attgtttctt gactgcggag ggggaactac gcctcggcga ctttggactt agtcgtgtat gggatacgga agcatccatg actcacgaag tcgtttcgag gtggtaccgg gctcccgagt tacttctggg ccagcgacac tacggacccg aaattgatgt gtggtcatca gggtgcgtat ttgcggaact tctacggggg tacggtggtg cttttttttg caggcgatgg agatattagg cagttgccac gaatatttga tgtattaggg acaccgacgc ccacatcgtg gccatcggcg gtgctgcttc ccgattgggg aaaggtgtac tttgagccga agcgtactct tcctctccgt gaatttttcc cggatgcctc tgaatgtgca ttggatttgt tgcgaagatt actttgcctg gatcctcgga agcgtttgac tgcggctggc gcccttcgtc acgcctactt tagtgaatac ccgacgtaag tgagtaanan aggatc

Expected Protein Sequence

MAHHHHHHMD RFIVGSPIGE GRFGVVRAAV VKESGVPAAI KCITTPRLDE GIPHPTAREL LVAMRLVHPF VIRTYEVVPC GCSLALVTER CKTDLATVLS GRTASNPLPA LEAKSLFKML LLALAYIHSE GVLHRDVKPS NCFLTAEGEL RLGDFGLSRV WDTEASMTHE VVSRWYRAPE LLLGQRHYGP EIDVWSSGCV FAELLRGYGG AFFAGDGDIW QLSRILDVLG TPTPTSWPSA VLLPDWGKLH FEPKRPLPLR EFFPDASECA LDLLRRLLCL DPQKRLTAAG ALCHAYFSEY PT

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatc gttttattgt tggatcccca attggtgagg gccgctttgg cgtcgttcgt gctgcagttg ttaaggaatc tggagtgcca gccgcaatca aatgcatcac aactccacgt ctcgatgagg gtattccgca ccccacagcg agggaactgc tcgttgcaat gcgattggtg catccttttg ttattcggac atatgaggta gttccgtgtg gctgctcctt ggcgttagtg acggagcgtt gcaagactga cttggcaaca gtcctctcgg ggagaactgc cagcaatcct ctgcccgcgt tagaggcaaa gtctttattt aaaatgcttc ttttggcgtt agcgtacata cacagcgagg gtgttcttca tcgagatgtg aaaccttcaa attgtttctt gactgcggag ggggaactac gcctcggcga ctttggactt agtcgtgtat gggatacgga agcatccatg actcacgaag tcgtttcgag gtggtaccgg gctcccgagt tacttctggg ccagcgacac tacggacccg aaattgatgt gtggtcatca gggtgcgtat ttgcggaact tctacgaggg tacggtggtg ctttttttgc aggcgatgga gatatttggc agttgtcacg aatattagat gtattaggga caccgacgcc cacatcgtgg ccatcggcgg tgctgcttcc cgattgggga aagttgcact ttgagccgaa gcgtcctctt cctctccgtg aatttttccc ggatgcctct gaatgtgcat tggatttgtt gcgaagatta ctttgcctgg atcctcagaa gcgtttgact gcggctggcg ccctttgtca cgcctacttt agtgaatacc cgacgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat