SSGCID: Target Page

TrcrA.00761.e

protein kinase, putative,cdc2-related related kinase, putative

CENTER ID:	TrcrA.00761.e
ORGANISM:	Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.00761.e.B1.GE35117	full length		1	294

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tc00.1047053509163.70
RefSeq:	XP_812962.1
UniProt:	Q4DF08

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDRFIIGSPI GEGRFGVVRA AVVKESGMPA AIKFVTTPRL DEGIPHPTAR GLLVAMRLVH PFVIRTYEVV PCGCSLALVM ERCKTDLAAV LSGRTASNPL PALEAKSLFK MLLLALAYIH SEGVLHRDVK PSNCFLTAEG ELRLGDFGLS RVWDTEASMT HEVVSRWYRA PELLLGQRHY GPEIDVWSSG CVFAELLRGY SGALFAGDGD IWQLSRIFDV LWTPTPTSWP SAVLLPDWGK VHFEPKRPLP LREFFPDASE CALDLLRRLL CLDPQKRLTA AGALRHAYFG EYPT

NT Sequence

atggatcgtt ttattattgg atccccaatt ggtgagggcc gctttggcgt cgttcgtgct gcagttgtta aggaatctgg aatgccagcc gcaatcaaat tcgtcacaac tccacgtctc gatgagggta ttccgcatcc cacagcgagg ggactgcttg ttgcaatgcg attggtgcat ccttttgtta ttcggacata tgaggtagtt ccgtgtggct gctccttggc gttagtgatg gagcgttgca agactgactt ggcagcagtc ctctcaggga gaactgccag caatcctctg cccgcgttag aggcaaagtc tttatttaaa atgcttcttt tggcgttggc gtacatacac agcgagggtg ttcttcatcg agatgtgaaa ccttcaaatt gtttcttgac tgcggagggg gaactacgcc tcggcgactt tggactcagt cgtgtatggg atacggaggc atccatgact cacgaagtcg tttcgaggtg gtaccgagct cccgagttac ttctgggcca gcgacactac ggacccgaga ttgatgtgtg gtcatcagga tgcgtatttg cggaacttct acgagggtac agtggtgctc tttttgcggg cgatggagat atttggcagt tgtcacgaat atttgatgta ttatggacac cgacgcccac atcgtggcca tcggcggtgc tgcttcccga ttggggaaag gtgcactttg agccgaagcg tcctcttcct ctccgtgaat ttttcccgga tgcctctgaa tgtgcattgg atttgttgcg aagattactt tgcctggatc ctcaaaagcg tttgactgcg gctggcgccc ttcgtcacgc ctactttggt gaatacccga cgtga

Details for TrcrA.00761.e.B1.GE35117

HARVESTED ON:	4/10/2012
SEQUENCED ON:	4/11/2012
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	96
PERCENT COVERAGE:	83

Validated AA Sequence

MAHHHHHHMD RFIIGSPIGE GRFGVVCAAV VKESGMPAAI KFVTTPRLDE GIPPPTAREL LVAMRLVHPF VIRKYEVVPC GCSLALVMER CKTDLATVLS GRTASNPLPA LEAKSLFKML LLALAYIHSE GVLHRDVKPS NCFLTAEGEL RLGDFGLSRV WDTEASMTHE VVSRWYRAPE LLLGQRHYGP EIDVWSSECV FAELLRGYSG AFFAGDGDIW QLSRIFDVLG TPTPTSWPSA VLLPDWGKGA L

Validated NT Sequence

tnnnnnnnnn nnntttgttt aactttaaga aggagatata ccatggctca ccaccaccac caccatatgg atcgttttat tattggatcc ccaattggtg agggccgctt tggcgtcgtt tgtgctgcag ttgttaagga atctggaatg ccagccgcaa tcaaattcgt cacaactcca cgtctcgatg agggtattcc gcctcccaca gcgagggaac tacttgttgc aatgcgattg gtgcatcctt ttgttattcg gaaatatgag gtagttccgt gtggctgctc cttggcgtta gtgatggagc gttgcaagac tgacttggca acagtcctct cagggagaac tgccagcaat cctctgcccg cgttagaggc aaagtctttg tttaaaatgc ttcttttggc gttagcgtac atacacagcg aaggtgttct tcatcgagat gtgaaacctt caaattgttt cttgactgcg gagggggaac tacgcctcgg cgactttgga ctcagtcgtg tatgggatac ggaggcatcc atgactcacg aagtcgtttc gaggtggtac cgagctcccg agttacttct gggccagcga cactacggac ccgagattga tgtgtggtca tcagagtgcg tatttgcgga acttctacga gggtacagtg gtgctttttt tgcgggcgat ggagatattt ggcagttgtc acgaatattt gatgtattag ggacaccgac gcccacatcg tggccatcgg cggtgctgct tcccgattgg gggaaaggtg cactttgagc cgaagcgtcc tcttcctctc cgtgaatttt tcccggatgc ctctgaatgt gcattgnatt tgttgcgaan gttactttgc ctggaacctc aaaagcgttt gactgcggct gccgcccttc gccacgccta ctttggtgaa tacccgacgt aagtgagtaa nanagnatnn nntgnnnnnn nnncccnna

Expected Protein Sequence

MAHHHHHHMD RFIIGSPIGE GRFGVVRAAV VKESGMPAAI KFVTTPRLDE GIPHPTARGL LVAMRLVHPF VIRTYEVVPC GCSLALVMER CKTDLAAVLS GRTASNPLPA LEAKSLFKML LLALAYIHSE GVLHRDVKPS NCFLTAEGEL RLGDFGLSRV WDTEASMTHE VVSRWYRAPE LLLGQRHYGP EIDVWSSGCV FAELLRGYSG ALFAGDGDIW QLSRIFDVLW TPTPTSWPSA VLLPDWGKVH FEPKRPLPLR EFFPDASECA LDLLRRLLCL DPQKRLTAAG ALRHAYFGEY PT

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatc gttttattat tggatcccca attggtgagg gccgctttgg cgtcgttcgt gctgcagttg ttaaggaatc tggaatgcca gccgcaatca aattcgtcac aactccacgt ctcgatgagg gtattccgca tcccacagcg aggggactgc ttgttgcaat gcgattggtg catccttttg ttattcggac atatgaggta gttccgtgtg gctgctcctt ggcgttagtg atggagcgtt gcaagactga cttggcagca gtcctctcag ggagaactgc cagcaatcct ctgcccgcgt tagaggcaaa gtctttattt aaaatgcttc ttttggcgtt ggcgtacata cacagcgagg gtgttcttca tcgagatgtg aaaccttcaa attgtttctt gactgcggag ggggaactac gcctcggcga ctttggactc agtcgtgtat gggatacgga ggcatccatg actcacgaag tcgtttcgag gtggtaccga gctcccgagt tacttctggg ccagcgacac tacggacccg agattgatgt gtggtcatca ggatgcgtat ttgcggaact tctacgaggg tacagtggtg ctctttttgc gggcgatgga gatatttggc agttgtcacg aatatttgat gtattatgga caccgacgcc cacatcgtgg ccatcggcgg tgctgcttcc cgattgggga aaggtgcact ttgagccgaa gcgtcctctt cctctccgtg aatttttccc ggatgcctct gaatgtgcat tggatttgtt gcgaagatta ctttgcctgg atcctcaaaa gcgtttgact gcggctggcg cccttcgtca cgcctacttt ggtgaatacc cgacgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat