TrcrA.00761.b

protein kinase, putative

CENTER ID: TrcrA.00761.b
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.00761.b.B1.GE35114 full length 1 339
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.00761.b.B1.PS01538 full length 1 339

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053510525.10
RefSeq: XP_810108.1
UniProt: Q4D6W8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGRVGEYEIF ETVGTGAFSK VKRVRHIPTD ACFVAKIIPK SNQRVENDIR VEISILRRLK HRNIVQLVEI LESPNNYYII LEPVFGGDVC SLVLSQDSSL EEEVVSGLFF QLLAGVRACH QNGVAHRDLK PENLLLTEGN VLKISDFGLS RLHKQSNFHA QIDEYAHTLT GTLAYVAPEV FCGTYDAFKA DIWSMGCILY VLLTSCFPFG YTTDARELEE RIKMGRICKL PDSVSEEAKE LTLWLMSLHP EDRPSLDQVA LHSFLKRYVP MKHILALIGS KQPPPLAANP AEFSSEVVEE EKQVNERGRP LFGKEAPREY SLKPCSGGSS KPMGQSRKD
NT Sequence
atgggaaggg ttggtgaata cgaaatattt gaaaccgtcg gtacaggcgc gtttagcaaa gttaaaagag taagacatat tcctactgac gcatgttttg tggcgaaaat aatacccaag agcaatcaac gtgtagaaaa tgacattcgt gtggaaattt cgattcttcg tagattaaag catcgcaata ttgttcaatt ggtagaaatt ttggagagtc ctaataatta ttacattatt cttgagcccg tctttggagg agatgtttgt agtctagttc tttctcaaga tagctccctt gaagaagagg tggtatctgg tttgtttttt caacttctag ctggtgttcg tgcttgccac caaaatggtg tggcacatag ggacttaaaa ccagaaaatc tccttttaac tgaaggaaat gtgctgaaaa tatccgattt tggactaagt cggctacata aacaaagcaa cttccacgca caaattgatg agtatgccca tacgcttacc ggaacccttg cttatgtggc accggaagtt ttttgcggta cctatgatgc ttttaaagcg gatatttggt ctatggggtg cattctttac gttttattga ccagttgctt tccgtttgga tatacaacag atgcaagaga gcttgaagaa cgtattaaaa tgggacgtat ttgcaagctg cctgattcag ttagtgaaga ggcaaaagag ttgactttgt ggcttatgtc actccatcca gaagatagac cctcgttgga ccaggtagcg ttgcattctt ttttgaaaag gtatgtgccg atgaaacata ttttggcact catagggagc aaacaaccac cacccctcgc agcgaaccct gccgaattta gtagtgaggt tgttgaagaa gaaaaacaag tcaacgaaag aggtcgccct ctttttggta aagaggcacc tcgagaatat agtttgaaac catgttcagg tgggtcttct aaaccaatgg gtcaatcaag gaaggattga
Details for TrcrA.00761.b.B1.GE35114
HARVESTED ON: 4/10/2012
SEQUENCED ON: 4/11/2012
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 41
Validated AA Sequence
MGCILYVLLT SCFPFGYTTD ARELEERIKM GRICKLPDSV SEEAKELTLW LMSLHPEDRP SLDQVALHSF LKRYVPMKHI LALIGSKQPP PLAANPAEFS SEVVEEEKQV NERGRPLFGK EAPREYSLKP CSGGSSKPMG QSR
Validated NT Sequence
tccttgattg acccattggt ttagaagacc cacctgaaca tggtttcaaa ctatattctc gaggtgcctc tttaccaaaa agagggcgac ctctttcgtt gacttgtttt tcttcttcaa caacctcact actaaattcg gcagggttcg ctgcgagggg tggtggttgt ttgctcccta tgagtgccaa aatatgtttc atcggcacat accttttcaa aaaagaatgc aacgctacct ggtccaacga gggtctatct tctggatgga gtgacataag ccacaaagtc aactcttttg cctcttcact aactgaatca ggcagcttgc aaatacgtcc cattttaata cgttcttcaa gctctcttgc atctgttgta tatccaaacg gaaagcaact ggtcaataaa acgtaaagaa tgcaccccat agaccaaata tccgctttaa aagcatcata ggtaccgcaa aaaacttccg gtgccacata agcaagggtt ccggtaagcg tatgggcata ctcatcaatt tgtgcgtgga agttgctttg tttatgtagc cgacttagtc caaaatcgga tattttcagc acatttcctt cagttaaaag gagattttct ggttttaagt ccctatgtgc cacaccattt tggtggcaag cacgaacacc agctagaagt tgaaaaaaca aaccagatac cacctcttct tcaagggagc tatcttgaga aagaactaga ctacaaacat ctcctccaaa gacgggctca agaataatgt aataattatt aggactctcc aaaatttcta ccaattgaac aatattgcga tgctttaatc tacgaagaat cgaaatttcc acacgaatgt cattttctac acgttgattg ctcttgggta ttattttcgc cacaaaacat gcgtcagtag gaatatgtct tactctttta actttgctaa acgcgcctgt accgacggtt tcaaatattt cgtattcacc aaccc
Expected Protein Sequence
MAHHHHHHMG RVGEYEIFET VGTGAFSKVK RVRHIPTDAC FVAKIIPKSN QRVENDIRVE ISILRRLKHR NIVQLVEILE SPNNYYIILE PVFGGDVCSL VLSQDSSLEE EVVSGLFFQL LAGVRACHQN GVAHRDLKPE NLLLTEGNVL KISDFGLSRL HKQSNFHAQI DEYAHTLTGT LAYVAPEVFC GTYDAFKADI WSMGCILYVL LTSCFPFGYT TDARELEERI KMGRICKLPD SVSEEAKELT LWLMSLHPED RPSLDQVALH SFLKRYVPMK HILALIGSKQ PPPLAANPAE FSSEVVEEEK QVNERGRPLF GKEAPREYSL KPCSGGSSKP MGQSRKD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggaa gggttggtga atacgaaata tttgaaaccg tcggtacagg cgcgtttagc aaagttaaaa gagtaagaca tattcctact gacgcatgtt ttgtggcgaa aataataccc aagagcaatc aacgtgtaga aaatgacatt cgtgtggaaa tttcgattct tcgtagatta aagcatcgca atattgttca attggtagaa attttggaga gtcctaataa ttattacatt attcttgagc ccgtctttgg aggagatgtt tgtagtctag ttctttctca agatagctcc cttgaagaag aggtggtatc tggtttgttt tttcaacttc tagctggtgt tcgtgcttgc caccaaaatg gtgtggcaca tagggactta aaaccagaaa atctcctttt aactgaagga aatgtgctga aaatatccga ttttggacta agtcggctac ataaacaaag caacttccac gcacaaattg atgagtatgc ccatacgctt accggaaccc ttgcttatgt ggcaccggaa gttttttgcg gtacctatga tgcttttaaa gcggatattt ggtctatggg gtgcattctt tacgttttat tgaccagttg ctttccgttt ggatatacaa cagatgcaag agagcttgaa gaacgtatta aaatgggacg tatttgcaag ctgcctgatt cagttagtga agaggcaaaa gagttgactt tgtggcttat gtcactccat ccagaagata gaccctcgtt ggaccaggta gcgttgcatt cttttttgaa aaggtatgtg ccgatgaaac atattttggc actcataggg agcaaacaac caccacccct cgcagcgaac cctgccgaat ttagtagtga ggttgttgaa gaagaaaaac aagtcaacga aagaggtcgc cctctttttg gtaaagaggc acctcgagaa tatagtttga aaccatgttc aggtgggtct tctaaaccaa tgggtcaatc aaggaaggat tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for TrcrA.00761.b.B1.PS01538
PURIFICATION DATe: 6/26/2012
CONCENTRATION: 91.1mg/ml
OBSERVED MW: 37kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 41
Protocol Notes
notes unavailable
Validated AA Sequence
MGCILYVLLT SCFPFGYTTD ARELEERIKM GRICKLPDSV SEEAKELTLW LMSLHPEDRP SLDQVALHSF LKRYVPMKHI LALIGSKQPP PLAANPAEFS SEVVEEEKQV NERGRPLFGK EAPREYSLKP CSGGSSKPMG QSR
Validated NT Sequence
tccttgattg acccattggt ttagaagacc cacctgaaca tggtttcaaa ctatattctc gaggtgcctc tttaccaaaa agagggcgac ctctttcgtt gacttgtttt tcttcttcaa caacctcact actaaattcg gcagggttcg ctgcgagggg tggtggttgt ttgctcccta tgagtgccaa aatatgtttc atcggcacat accttttcaa aaaagaatgc aacgctacct ggtccaacga gggtctatct tctggatgga gtgacataag ccacaaagtc aactcttttg cctcttcact aactgaatca ggcagcttgc aaatacgtcc cattttaata cgttcttcaa gctctcttgc atctgttgta tatccaaacg gaaagcaact ggtcaataaa acgtaaagaa tgcaccccat agaccaaata tccgctttaa aagcatcata ggtaccgcaa aaaacttccg gtgccacata agcaagggtt ccggtaagcg tatgggcata ctcatcaatt tgtgcgtgga agttgctttg tttatgtagc cgacttagtc caaaatcgga tattttcagc acatttcctt cagttaaaag gagattttct ggttttaagt ccctatgtgc cacaccattt tggtggcaag cacgaacacc agctagaagt tgaaaaaaca aaccagatac cacctcttct tcaagggagc tatcttgaga aagaactaga ctacaaacat ctcctccaaa gacgggctca agaataatgt aataattatt aggactctcc aaaatttcta ccaattgaac aatattgcga tgctttaatc tacgaagaat cgaaatttcc acacgaatgt cattttctac acgttgattg ctcttgggta ttattttcgc cacaaaacat gcgtcagtag gaatatgtct tactctttta actttgctaa acgcgcctgt accgacggtt tcaaatattt cgtattcacc aaccc
Expressed Protein Sequence
MAHHHHHHMG RVGEYEIFET VGTGAFSKVK RVRHIPTDAC FVAKIIPKSN QRVENDIRVE ISILRRLKHR NIVQLVEILE SPNNYYIILE PVFGGDVCSL VLSQDSSLEE EVVSGLFFQL LAGVRACHQN GVAHRDLKPE NLLLTEGNVL KISDFGLSRL HKQSNFHAQI DEYAHTLTGT LAYVAPEVFC GTYDAFKADI WSMGCILYVL LTSCFPFGYT TDARELEERI KMGRICKLPD SVSEEAKELT LWLMSLHPED RPSLDQVALH SFLKRYVPMK HILALIGSKQ PPPLAANPAE FSSEVVEEEK QVNERGRPLF GKEAPREYSL KPCSGGSSKP MGQSRKD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGAA GGGTTGGTGA ATACGAAATA TTTGAAACCG TCGGTACAGG CGCGTTTAGC AAAGTTAAAA GAGTAAGACA TATTCCTACT GACGCATGTT TTGTGGCGAA AATAATACCC AAGAGCAATC AACGTGTAGA AAATGACATT CGTGTGGAAA TTTCGATTCT TCGTAGATTA AAGCATCGCA ATATTGTTCA ATTGGTAGAA ATTTTGGAGA GTCCTAATAA TTATTACATT ATTCTTGAGC CCGTCTTTGG AGGAGATGTT TGTAGTCTAG TTCTTTCTCA AGATAGCTCC CTTGAAGAAG AGGTGGTATC TGGTTTGTTT TTTCAACTTC TAGCTGGTGT TCGTGCTTGC CACCAAAATG GTGTGGCACA TAGGGACTTA AAACCAGAAA ATCTCCTTTT AACTGAAGGA AATGTGCTGA AAATATCCGA TTTTGGACTA AGTCGGCTAC ATAAACAAAG CAACTTCCAC GCACAAATTG ATGAGTATGC CCATACGCTT ACCGGAACCC TTGCTTATGT GGCACCGGAA GTTTTTTGCG GTACCTATGA TGCTTTTAAA GCGGATATTT GGTCTATGGG GTGCATTCTT TACGTTTTAT TGACCAGTTG CTTTCCGTTT GGATATACAA CAGATGCAAG AGAGCTTGAA GAACGTATTA AAATGGGACG TATTTGCAAG CTGCCTGATT CAGTTAGTGA AGAGGCAAAA GAGTTGACTT TGTGGCTTAT GTCACTCCAT CCAGAAGATA GACCCTCGTT GGACCAGGTA GCGTTGCATT CTTTTTTGAA AAGGTATGTG CCGATGAAAC ATATTTTGGC ACTCATAGGG AGCAAACAAC CACCACCCCT CGCAGCGAAC CCTGCCGAAT TTAGTAGTGA GGTTGTTGAA GAAGAAAAAC AAGTCAACGA AAGAGGTCGC CCTCTTTTTG GTAAAGAGGC ACCTCGAGAA TATAGTTTGA AACCATGTTC AGGTGGGTCT TCTAAACCAA TGGGTCAATC AAGGAAGGAT TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT