SSGCID: Target Page

TrcrA.00703.a

protein kinase A catalytic subunit

CENTER ID:	TrcrA.00703.a
ORGANISM:	Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.00703.a.B1.GE35111	full length		1	331

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tc00.1047053508461.280
RefSeq:	XP_821620.1
UniProt:	Q4E4T3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTLKNAPEFV KPDASNWKLT DLEMGSTLGA GSFGRVRIAK LKGTNDYYAV KCLKKREILK MKQVQHIRQE KQILMELSHP FIVNMMCSFQ DDRRVYFVLE FVVGGEMFTH LRSAGRFPND VAKFYHAEIV LAFEYLHSKD IIYRDLKPEN LLLDSKGHVK VTDFGFAKKV PERTFTLCGT PEYLAPEVIQ SKGHGKAVDW WTMGVLLYEF IAGYPPFYDD TPFRTYEKIL SGRFKFPSWF DARARDLVKG LLQTDHTKRL GTLKGGVADV KNHPYFHGAN WEKLYARYYP APIPVKAKSP GDTSNFERYP ESREDRVVPL TATQQAEFIG F

NT Sequence

atgacgttga agaatgcacc tgaatttgta aagccggacg cctcaaattg gaagttaaca gaccttgaga tggggagtac gttaggggcg ggttcttttg gtcgcgtgcg cattgctaaa ctgaagggca ccaatgacta ttatgcggtg aagtgcttga agaagcggga gattttaaag atgaaacagg tccaacacat caggcaagaa aagcaaattt taatggagct ttcacacccg ttcattgtga acatgatgtg ttcctttcag gatgatcgcc gggtgtactt tgtgttggag tttgtggtgg gcggggaaat gtttactcac cttcgctccg ccggtcggtt tccaaacgat gtagcaaagt tctaccacgc ggagattgtg cttgccttcg aatacctgca ctcgaaggac atcatctacc gtgacctgaa gccggagaac cttctgctgg acagcaaggg ccacgtgaag gtgactgact ttgggtttgc gaagaaggtg ccggaacgta ccttcacgct gtgtgggaca ccggagtacc ttgcgcccga ggtgattcag agcaagggtc atgggaaggc cgtggactgg tggacgatgg gcgttctgct gtacgaattc attgctgggt atcctccgtt ttacgacgac acgccgttcc gcacgtacga gaagatcctc tctgggcgct tcaagtttcc gagttggttt gatgcgcgtg cgcgggatct tgtgaagggg cttctgcaaa cggaccacac gaagcgtttg ggcacactca agggcggcgt agcggatgtg aagaatcacc cgtactttca cggtgccaac tgggagaagt tgtatgcgcg atactaccct gccccgatcc ctgtgaaggc aaagagccct ggcgacacga gtaactttga gcgttaccct gaaagtcgtg aagatcgagt ggtgccgcta accgctacgc aacaggcgga gtttattggg ttctaa

Details for TrcrA.00703.a.B1.GE35111

HARVESTED ON:	4/10/2012
SEQUENCED ON:	4/11/2012
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	97
PERCENT COVERAGE:	96

Validated AA Sequence

MAHHHHHHMT LKNAPEFVKP DASNWKLTDL EMGSTLGAGS FGRVRIAKLK GTNDYYAVKC LKKREILKMK QVQHIRQEKQ ILMELSHPFI VNMMCSFQDD RRVYFVLEFV VGGEMFTHLR SAGRFPNDVA KFYHAEIVLA FEYLHSKDII YRDLKPENLL LDSKGHVKVT DFGFAKKVPE RTFTLCGTPE YLAPEVIQSK GHGKAVDWWT MGVLLYEFIA GYPPFYDDTP FRTYEKILSG RFKFPSWFDA RARDLVKGLL QTDHTKRLGT LKGGVADVKN HPYFHGANWX KLYARYYPAP IPVXAKSXGX XSNFERYPES RXIERCR

Validated NT Sequence

attttgtnnn nnttaagaag gagatatacc atggctcacc accaccacca ccatatgacg ttgaagaatg cacctgaatt tgtgaagccg gacgcctcaa attggaagtt aacagacctt gagatgggga gcacgttagg ggcgggttct tttggtcgcg tgcgcattgc taagctgaag ggcaccaatg actattatgc ggtgaagtgc ttgaagaagc gggagatttt aaagatgaag caggtccaac acataagaca agaaaagcaa attttaatgg agctttcaca cccgttcatt gtgaacatga tgtgttcctt tcaggatgat cgccgggtgt actttgtgtt ggagtttgtg gtgggcgggg aaatgtttac tcaccttcgc tccgccggtc ggtttccaaa cgatgtagca aagttctacc acgcggagat tgtgcttgcc ttcgaatacc tgcactcgaa ggacatcatc taccgcgacc tgaagccgga gaaccttctg ctggacagca agggccacgt gaaggtgact gactttgggt ttgcgaagaa ggtgccggag cgtaccttca cgctgtgtgg gacaccggag taccttgcgc ccgaggtgat tcagagcaag ggtcatggga aggccgtgga ctggtggacg atgggcgttc tgctgtacga atttattgct gggtatcctc cgttttacga cgacacgccg ttccgcacgt acgagaagat cctctctggg cgcttcaagt ttccgagttg gtttgatgcg cgtgcgcggg atcttgtgaa ggggcttctg caaacggacc acacgaagcg tttgggcaca ctcaaaggcg gcgtagcgga tgtgaagaat cacccgtact ttcacggtgc caactgggnn aagttgtatg cgcgatacta ccctgcaccg atccctgtga nggcaaagag ccntggcnac ncgagtaact ttgagcgtta ccctgaaagt cgtganatcg agcgctgccg ctaaccgcta cncnttnant nggntnctan tgantanatn gnannnnnnn nnnaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgt tgaagaatgc acctgaattt gtaaagccgg acgcctcaaa ttggaagtta acagaccttg agatggggag tacgttaggg gcgggttctt ttggtcgcgt gcgcattgct aaactgaagg gcaccaatga ctattatgcg gtgaagtgct tgaagaagcg ggagatttta aagatgaaac aggtccaaca catcaggcaa gaaaagcaaa ttttaatgga gctttcacac ccgttcattg tgaacatgat gtgttccttt caggatgatc gccgggtgta ctttgtgttg gagtttgtgg tgggcgggga aatgtttact caccttcgct ccgccggtcg gtttccaaac gatgtagcaa agttctacca cgcggagatt gtgcttgcct tcgaatacct gcactcgaag gacatcatct accgtgacct gaagccggag aaccttctgc tggacagcaa gggccacgtg aaggtgactg actttgggtt tgcgaagaag gtgccggaac gtaccttcac gctgtgtggg acaccggagt accttgcgcc cgaggtgatt cagagcaagg gtcatgggaa ggccgtggac tggtggacga tgggcgttct gctgtacgaa ttcattgctg ggtatcctcc gttttacgac gacacgccgt tccgcacgta cgagaagatc ctctctgggc gcttcaagtt tccgagttgg tttgatgcgc gtgcgcggga tcttgtgaag gggcttctgc aaacggacca cacgaagcgt ttgggcacac tcaagggcgg cgtagcggat gtgaagaatc acccgtactt tcacggtgcc aactgggaga agttgtatgc gcgatactac cctgccccga tccctgtgaa ggcaaagagc cctggcgaca cgagtaactt tgagcgttac cctgaaagtc gtgaagatcg agtggtgccg ctaaccgcta cgcaacaggc ggagtttatt gggttctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t