TrcrA.00519.a

serine acetyltransferase, putative

CENTER ID: TrcrA.00519.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.00519.a.B1.GE35105 full length 1 341
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053504013.40
RefSeq: XP_810043.1
UniProt: Q4D6P5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNRLEVIRKS ISYVGLDPAM FKPYPFNVSS SALSELMDCM SYIVFPEFSE PPMNPVTVPE QRLGGSALLQ WTLTRMADII NSQIYSALML QGHTKCSKDE AGIPHSLNAE SGSNDDGFIP QNETHAFVEV RSFHLEAKKK AEEITEAFLM RKLRNIRWLL RTDAESILNN DIASNSLSEV VLCYPGLRCM KHQRTAHALH ELGAPSNFTR LLTEMAHSTT GIDIHPATSI GHHFFIDHGT GIVIGSTAII GNHVSIYHGV TLGTRSFPVV AKTGERVRNL ARHPIIEDRV TIYANAMVLG RIRIGKDSVI GSYCLVLKDV PPKSMIRRTP NAKLPTPYIT I
NT Sequence
atgaatcgac tcgaagtcat tcggaagtca atttcatatg ttggcctcga tccggccatg ttcaaacctt acccttttaa tgtcagtagt tccgccctct cagaactgat ggactgcatg tcctacattg tattccccga attcagtgaa cccccaatga atcctgtgac tgtccccgag caacgccttg gtggcagtgc actgcttcaa tggacactga cccgcatggc cgacattatc aactcacaga tttattctgc ccttatgctt cagggccaca cgaaatgctc taaggatgaa gcaggtattc cccactcgtt aaatgctgag agcgggtcaa atgatgatgg ttttatacct caaaatgaaa ctcatgcctt tgtcgaagtc agatcttttc atttggaagc caagaaaaag gcggaagaga tcacagaagc ttttctaatg cgtaaattac gcaacatacg gtggcttctt cgtaccgatg cagaaagtat ccttaataat gatattgcat ctaactctct ctcggaagtg gttctttgct atcccggact acgctgcatg aaacaccagc gcactgcaca tgcgttacac gaactggggg ctccaagcaa ttttacgcgc cttcttacag aaatggcgca ttccacgacc ggcattgaca tccatcctgc tacctctatt ggccatcatt tttttattga tcatggtact ggaattgtga ttgggtcaac cgccatcatt ggcaaccacg tatctatata tcatggggtc acgctgggga caaggtcgtt tcctgtggtt gctaaaactg gggagagggt gaggaacctt gcacgccacc ccattattga ggacagagtg accatctacg ccaacgctat ggtgcttggc cgaattcgga tagggaagga tagcgtcatt ggtagttact gcctggtctt gaaggacgta cctcccaaat ccatgattcg gcggactccc aatgcaaaac tccctactcc atatataacc atttaa
Details for TrcrA.00519.a.B1.GE35105
HARVESTED ON: 4/10/2012
SEQUENCED ON: 4/11/2012
EXPECTED MW: 38kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 94
PERCENT COVERAGE: 90
Validated AA Sequence
MAHHHHHHMN RLEVIRKSIS YVGLDPAMFK PYPFNVSSSA LSELMDCMSY IVFPEFSEPP MNPVTVPEQR LGGSALLQWT LTRMADIINS QIYSALMLQG HTKCSKDEAG IPHSLNAESG SNDDGFTPQN ETHAFGEVRS FHLEAKKRAE EITEAFLTRK LRSIRWLLRT DAESILNNDI ASNSLSEVVL CYPGLRCMKH QRTAHALHEL GAPSIFTRLL TEMAHSTTGI DIHPATSIGH HFFIDHGTGI VIGSTAIIGN YVSIYHGVTL GTRSFPVVAK TGEKVXNLAR HPXIEDRVTI YANAMVLGXN SDRXX
Validated NT Sequence
tttgtttanc tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatc gactcgaagt cattcggaag tcaatttcat atgttggcct cgatccggcc atgttcaaac cttacccttt taatgtcagt agttctgccc tctcagaact gatggactgc atgtcctaca ttgtattccc cgaattcagt gaacccccaa tgaatcctgt gactgtcccc gagcaacgcc ttggcggcag tgcactgctt caatggacac tgacccgcat ggccgacatt atcaactcac agatttattc tgcccttatg cttcagggcc acacgaaatg ctctaaagat gaagcaggta ttccccactc gttaaatgct gagagcgggt caaatgatga tggttttaca cctcaaaatg aaactcatgc ctttggcgaa gttagatctt ttcatttgga agccaagaaa agggcggaag agatcacaga agcttttcta acgcgtaaat tacgcagcat acggtggctt cttcgtaccg atgcagaaag tatccttaat aatgatattg catccaactc tctctcggaa gtggttcttt gctatcccgg actacgctgc atgaaacacc agcgcactgc acatgcgtta cacgaactgg gggctccaag catttttacg cgccttctta cagaaatggc gcattccacg accggcattg acatccatcc tgctacctct attggccatc atttttttat tgatcatggc actggtattg tgattgggtc aaccgccatc attggcaact acgtatctat ataccatggg gtcacgctgg ggacaaggtc ttttcctgtg gttgctaaaa ctggggagaa ggtgangaac cttgcacgcc accccnttat tgaggacaga gtgaccatct acgccaacgc tatggtgctt ggncnnaatt cngataggga nnnatagcgt cattggnagc nactgnctgg ncntgaagga cttaccttcn aaatccatga ttcgnnngan atnnnngcaa aactcccnac tccnnnnnnn nnntttngag tanannnnnc
Expected Protein Sequence
MAHHHHHHMN RLEVIRKSIS YVGLDPAMFK PYPFNVSSSA LSELMDCMSY IVFPEFSEPP MNPVTVPEQR LGGSALLQWT LTRMADIINS QIYSALMLQG HTKCSKDEAG IPHSLNAESG SNDDGFIPQN ETHAFVEVRS FHLEAKKKAE EITEAFLMRK LRNIRWLLRT DAESILNNDI ASNSLSEVVL CYPGLRCMKH QRTAHALHEL GAPSNFTRLL TEMAHSTTGI DIHPATSIGH HFFIDHGTGI VIGSTAIIGN HVSIYHGVTL GTRSFPVVAK TGERVRNLAR HPIIEDRVTI YANAMVLGRI RIGKDSVIGS YCLVLKDVPP KSMIRRTPNA KLPTPYITI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatc gactcgaagt cattcggaag tcaatttcat atgttggcct cgatccggcc atgttcaaac cttacccttt taatgtcagt agttccgccc tctcagaact gatggactgc atgtcctaca ttgtattccc cgaattcagt gaacccccaa tgaatcctgt gactgtcccc gagcaacgcc ttggtggcag tgcactgctt caatggacac tgacccgcat ggccgacatt atcaactcac agatttattc tgcccttatg cttcagggcc acacgaaatg ctctaaggat gaagcaggta ttccccactc gttaaatgct gagagcgggt caaatgatga tggttttata cctcaaaatg aaactcatgc ctttgtcgaa gtcagatctt ttcatttgga agccaagaaa aaggcggaag agatcacaga agcttttcta atgcgtaaat tacgcaacat acggtggctt cttcgtaccg atgcagaaag tatccttaat aatgatattg catctaactc tctctcggaa gtggttcttt gctatcccgg actacgctgc atgaaacacc agcgcactgc acatgcgtta cacgaactgg gggctccaag caattttacg cgccttctta cagaaatggc gcattccacg accggcattg acatccatcc tgctacctct attggccatc atttttttat tgatcatggt actggaattg tgattgggtc aaccgccatc attggcaacc acgtatctat atatcatggg gtcacgctgg ggacaaggtc gtttcctgtg gttgctaaaa ctggggagag ggtgaggaac cttgcacgcc accccattat tgaggacaga gtgaccatct acgccaacgc tatggtgctt ggccgaattc ggatagggaa ggatagcgtc attggtagtt actgcctggt cttgaaggac gtacctccca aatccatgat tcggcggact cccaatgcaa aactccctac tccatatata accatttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t