TrcrA.00436.b

protein kinase, putative

CENTER ID: TrcrA.00436.b
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.00436.b.B1.GE35100 full length 1 415
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053508205.30
RefSeq: XP_805706.1
UniProt: Q4CUA3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLFPFREEL HINQLYETDD IYDFIGRGTF ASVFKAVSLV DGEEVRKGDA VALKVFNKRD LTSEKMRLDV INEVEVLRRI NHKNCVRLLD CFQTPLHVVI VMNHVEGAEL FKALRDTVFT EAQVRFVAQQ LLEALDYLHN VVGVVHRDVK PENILVKPFG NDEFHVTLVD FGLARMLARR GGGHRRLGAT RLSLRALPEP TSVESIDSHC DSPLIATPCG TLNYAAPETV KSLTQSGGQL ATTPDLLARM DVYAVGTILY VMLVGKLPFR HSGNKMQLVK EMEAGPLFAE TRWSSVPREA IDLTKALLFF DPSKRPRARE ALQYAWFHPQ AAEEEGNGGG GGGGGSSSVG GLDDEGWQLR EFMGDREYMK LAFGAMKPPE SSFYDNGNSS VGGRCISVPF GSSRSSVKSY FGFCL
NT Sequence
atgtcgcttt ttccctttcg tgaggagctg cacatcaacc agctgtatga aacggatgac atctacgact ttattgggcg tggaacgttc gcgtcggtct tcaaggcggt gagtcttgtg gacggggaag aggtgcggaa gggcgacgcc gttgcgctca aggtgttcaa caagcgcgat ctcacctccg aaaagatgcg gctggatgtt atcaacgagg tggaggtgct ccgacggata aaccacaaga actgtgtgcg cttgttagac tgctttcaaa caccacttca tgttgttatt gtgatgaacc acgtggaagg cgcggagctg tttaaggctc ttcgtgacac ggtctttacg gaggcgcagg tgcgttttgt ggcgcagcag ttgcttgagg cattggatta tctccacaac gtcgttggtg ttgtgcaccg tgacgtaaaa ccagaaaata tattagtaaa gccctttggg aacgacgagt ttcatgtgac tttggtggac tttggtctgg cgcggatgct cgcccgcagg ggcggtggac accggcgttt gggagccaca cggctttcac tgcgtgcatt gccagagcca acatccgtgg aatccattga ttcccactgc gacagtccgc tcatcgcaac accgtgcgga acgctgaact acgcggcacc agaaacggtg aaaagtctca cgcagagcgg cgggcaactc gccacaacgc ccgacctcct cgctcgcatg gatgtatacg ccgtaggtac cattctgtac gttatgttgg ttggcaaact tccgttccgt cactccggaa acaagatgca acttgtcaag gagatggagg ctgggccact cttcgccgag acgcgatgga gcagcgtgcc tcgcgaggcc attgatctga cgaaggccct tcttttcttt gacccgtcaa agaggccgcg ggcgagggag gcgcttcagt acgcctggtt ccatccgcaa gcggctgagg aagagggtaa tggtggtggt ggtggtggtg gtggtagcag cagtgtgggt ggcttggacg atgagggctg gcaactgcgt gagttcatgg gagatcggga gtacatgaag ctggcgtttg gggcaatgaa gcccccagag agctcttttt acgacaatgg caactcctcc gttggcggcc gctgcattag tgtcccattt ggctcttcac gatcctccgt gaaatcatac tttggcttct gtctgtag
Details for TrcrA.00436.b.B1.GE35100
HARVESTED ON: 4/10/2012
SEQUENCED ON: 4/11/2012
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 81
PERCENT COVERAGE: 92
Validated AA Sequence
MAHHHHHHMS LFPFREELHI NQLYETDDIY DFIGRGTFAS VFKAVSLVDG EEVRKGDAVA LKVFNKRDLT SEKMRLDVIN EVEVLRRINH KNCVRLLDCF HTPLHVVIVM NHVEGAELFK ALRDTVFTEA QVRFVAQQLL EALDYLHNVV GVVHRDVKPE NILVKPFGND EFHVTLVDFG LARMLARRGG GHRRLGATRL SLRALPEPTS VESIDSHCDS PLIATPCGTL NYAAPETVKS LTQSGGQLAT TPDLLARMDV YAVXTILYVM LVGKLPFRHS GNKMQLVKXM EXGPLFAXTX WXSVPREAXD LTKALLFFDX SXXRGEGXLQ YXVXAXXXVX XXXAXXWXLX XXXXXXXWXX XXXXLXGXXX XXXXXXXXXX XXXXXXXXXX XX
Validated NT Sequence
tttgnttaac tttaagaagg aganatacca tggctcacca ccaccaccac catatgtcgc tttttccctt tcgtgaggag ctgcacatca accagctgta tgaaacggat gacatctacg actttattgg gcgtggaacg ttcgcgtcgg tcttcaaggc ggtgagtctt gtggacgggg aagaggtgcg gaagggcgac gccgttgcgc tcaaggtgtt caacaagcgc gacctcacct ccgaaaagat gcggctggat gttatcaacg aggtggaggt gctccggcgg ataaaccaca agaactgtgt gcgcttgtta gactgctttc acacaccact tcatgttgtt attgtgatga accacgtgga aggcgcggag ctctttaagg ctcttcgtga cacggtcttt acggaggcgc aggtgcgttt tgtggcgcaa cagttgcttg aggcgttgga ttatctccac aacgtcgttg gtgttgtgca ccgtgacgta aaaccagaaa atatattagt aaagcccttt gggaacgacg agtttcatgt gactttggtg gactttggtc tggcgcggat gctcgcccgc aggggcggtg gacaccggcg tttgggagcc acacggcttt cactgcgtgc attgccagag ccaacatccg tggaatccat tgattcccac tgcgacagtc cgctcatcgc aacaccatgc ggaacgctga actacgcggc accagaaacg gtgaaaagtc tcacgcagag cggcgggcaa ctcgccacaa cgcccgacct cctcgctcgc atggatgtat acgccgtnnn taccattctg tacgttatgt tggttggcaa acttccgttc cgtcactccg gaaacaagat gcaacttgtc aagganatgg agnctgggcc actcttcgcc ganacgcnat ggnncagcgt gcctcgcgag gccnttgatc tgacgaaggc ccttcttttc tttgaccngt caannnnncg nggngaggga gnncttcagt acnnngtcnn agcgntgana naggtangnn ngngngnagc nncanngtgg nngctnnnan nnnntgnant nnnngnntnn tggnnnnnnn gnnnnnnnnn nctnnnnggg nnannannnn nnnnnnnnnn nntncnnnnn gnnnnnnnnn cnnnnnnnnn nnnnnnnnnn nnnnnnnntn nnnant
Expected Protein Sequence
MAHHHHHHMS LFPFREELHI NQLYETDDIY DFIGRGTFAS VFKAVSLVDG EEVRKGDAVA LKVFNKRDLT SEKMRLDVIN EVEVLRRINH KNCVRLLDCF QTPLHVVIVM NHVEGAELFK ALRDTVFTEA QVRFVAQQLL EALDYLHNVV GVVHRDVKPE NILVKPFGND EFHVTLVDFG LARMLARRGG GHRRLGATRL SLRALPEPTS VESIDSHCDS PLIATPCGTL NYAAPETVKS LTQSGGQLAT TPDLLARMDV YAVGTILYVM LVGKLPFRHS GNKMQLVKEM EAGPLFAETR WSSVPREAID LTKALLFFDP SKRPRAREAL QYAWFHPQAA EEEGNGGGGG GGGSSSVGGL DDEGWQLREF MGDREYMKLA FGAMKPPESS FYDNGNSSVG GRCISVPFGS SRSSVKSYFG FCL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcgc tttttccctt tcgtgaggag ctgcacatca accagctgta tgaaacggat gacatctacg actttattgg gcgtggaacg ttcgcgtcgg tcttcaaggc ggtgagtctt gtggacgggg aagaggtgcg gaagggcgac gccgttgcgc tcaaggtgtt caacaagcgc gatctcacct ccgaaaagat gcggctggat gttatcaacg aggtggaggt gctccgacgg ataaaccaca agaactgtgt gcgcttgtta gactgctttc aaacaccact tcatgttgtt attgtgatga accacgtgga aggcgcggag ctgtttaagg ctcttcgtga cacggtcttt acggaggcgc aggtgcgttt tgtggcgcag cagttgcttg aggcattgga ttatctccac aacgtcgttg gtgttgtgca ccgtgacgta aaaccagaaa atatattagt aaagcccttt gggaacgacg agtttcatgt gactttggtg gactttggtc tggcgcggat gctcgcccgc aggggcggtg gacaccggcg tttgggagcc acacggcttt cactgcgtgc attgccagag ccaacatccg tggaatccat tgattcccac tgcgacagtc cgctcatcgc aacaccgtgc ggaacgctga actacgcggc accagaaacg gtgaaaagtc tcacgcagag cggcgggcaa ctcgccacaa cgcccgacct cctcgctcgc atggatgtat acgccgtagg taccattctg tacgttatgt tggttggcaa acttccgttc cgtcactccg gaaacaagat gcaacttgtc aaggagatgg aggctgggcc actcttcgcc gagacgcgat ggagcagcgt gcctcgcgag gccattgatc tgacgaaggc ccttcttttc tttgacccgt caaagaggcc gcgggcgagg gaggcgcttc agtacgcctg gttccatccg caagcggctg aggaagaggg taatggtggt ggtggtggtg gtggtggtag cagcagtgtg ggtggcttgg acgatgaggg ctggcaactg cgtgagttca tgggagatcg ggagtacatg aagctggcgt ttggggcaat gaagccccca gagagctctt tttacgacaa tggcaactcc tccgttggcg gccgctgcat tagtgtccca tttggctctt cacgatcctc cgtgaaatca tactttggct tctgtctgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat