TrcrA.00429.a

6-phospho-1-fructokinase, putative

CENTER ID: TrcrA.00429.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.00429.a.B1.GE35095 full length 1 485
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053508153.340
RefSeq: XP_822053.1
UniProt: Q4E657

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MENRLRDTSR VVRSHAAPLN EVTQEDLKVE RLHGRKYMNP SKKHVMREEF SDKIEHIMHD PRPQEGVHSE LPVSISPLLC ELAAPRQRIH FNPPETVVGI VTCGGICPGL NDVIRSLTLT AVNAYRVKRV IGFRFGYWGL SKKGSHTAME LYRTSVTSIH RYGGTILGSS RGPQDTSEMV DTLERLGVNI LFTVGGDGTQ RGALKIAEEA KRRGANLAVF GIPKTIDNDL SFSHRTFGFE TAVDKAVEAV RAAYAEAISL NYGVGVVKLM GRDSGFIAAE AAVASAQANI CLVPENPISE DIVMALIQRR FETSRSCVII VAEGFGQDWE GGTGGHDASG NKKLTDIGVV LTKRIQAWLR KNKERYPNGT VKYIDPSYMI RACPPSANDA LFCATLSTLA MHEAMAGATN CIIALRYNSY ILVPIKVATS VRRVLDLRGQ LWRQVREITV GLQDDVRAFK EAEVRRELEA ISLVRERLIG QLSKL
NT Sequence
atggaaaacc gccttcgtga cacgagcagg gttgtccgtt ctcacgcggc cccgctgaac gaggtcaccc aggaggacct aaaggtggag cgtctccatg gccgaaagta catgaacccc agcaaaaaac atgtgatgcg tgaggagttt tccgacaaga ttgaacatat catgcacgac ccccgtccac aggagggggt gcactcggaa cttcccgttt ccataagccc cctcctttgt gagcttgctg caccccgtca gcgtattcat ttcaatccac cggaaacagt tgtcggcatt gtcacatgcg gaggtatttg ccccggcttg aatgacgtga ttcgttcctt gactctgact gccgttaacg cataccgcgt gaagcgcgtc attggcttcc gatttggtta ctggggtctc tcaaagaagg gaagtcacac cgccatggag ctctaccgta catctgtcac aagcattcac cgttacggag gaaccatttt aggcagcagc cgcggtcccc aagacaccag tgaaatggtt gacaccctgg aacgtcttgg tgttaacatc ctctttactg ttggtggtga tggcacacaa cgtggtgctc ttaagattgc tgaagaggcc aagaggcggg gcgccaacct tgccgttttc ggtattccca agactattga caatgatctc agtttctcac accgcacttt tggttttgag acggcagtag acaaggctgt tgaggctgtc cgtgctgcat atgcggaagc tatttctctc aactacggtg ttggcgtcgt gaagctcatg ggccgtgaca gcggctttat tgcagcagaa gcagctgtgg caagtgccca ggcaaatatt tgccttgttc ctgaaaatcc tatttccgag gatatcgtaa tggcacttat ccaacgtcga tttgagacct cccgcagctg tgtcattatt gtggcagagg gctttggtca ggactgggaa gggggcactg gtggtcatga tgcctcgggc aataagaagc ttacggatat tggcgttgtc ctgacgaaga ggatacaagc atggctgcgt aaaaataaag agcgttaccc taatggcaca gtgaaataca ttgacccttc atacatgatt cgcgcctgtc cgccctcggc aaatgacgct cttttctgcg ccactctttc cacacttgcc atgcacgagg ctatggctgg tgcaacgaac tgtattattg cattgcgtta caatagctat attctcgtgc ctatcaaggt ggcaacctcg gttcgtcgtg tgttggatct ccgcggccag ctctggcgcc aagtgcgcga aatcactgtt ggcctgcagg atgacgtgcg tgccttcaaa gaagctgaag ttcgtcgtga actggaggcc atcagcctgg ttcgtgagcg cctgatcgga caactctcca agctgtaa
Details for TrcrA.00429.a.B1.GE35095
HARVESTED ON: 4/10/2012
SEQUENCED ON: 4/11/2012
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 84
Validated AA Sequence
MNPSKKHVMR EEFSDKIEHI MHDPRPQEGV HSELPVSISP LLCELAAPRQ RIHFNPPETV VGIVTCGGIC PGLNDVIRSL TLTAVNAYRV KRVIGFRFGY WGLSKKGSHT AMELYRTSVT SIHRYGGTIL GSSRGPQDTS EMVDTLERLG VNILFTVGGD GTQRGALKIA EEAKRRGVNI AVFGIPKTID NDLSFSHRTF GFETAVDKAV EAVRAAYAEA ISLNYGVGVV KLMGRDSGFI AAEAAVASAQ ANVCLVPENP ISEDIVMALI QRRFETSRSC VIIVAEGFGQ DWEGGTGGHD ASGNKKLTDI GVVLTKRIQA WLRKNKERYP NGTVKYIDPS YMIRACPPSA NDALFCATLS TLAMHEAMAG ATNCIIALRY NSYILVPIKV ATSVRRVLDL RGQLWRQVRE ITVGLQ
Validated NT Sequence
atcctgcagg ccaacagtga tttcgcgcac ttggcgccag agctggccgc ggagatccaa cacacgacga accgaagttg ccaccttgat aggcacgaga atatagctat tgtaacgcaa cgcaataata cagttcgttg caccagccat agcctcgtgc atggcaagtg tggaaagagt ggcgcagaaa agagcgtcat ttgccgaggg cggacaggcg cgaatcatgt atgaagggtc aatgtatttc accgtgccat tagggtaacg ctctttattt ttacgcagcc atgcttgaat cctcttcgtc aggacaacgc caatatccgt aagcttctta ttgcccgagg catcatgacc accagtgccc ccttcccagt cctgaccaaa gccctccgcc acaataatga cacagctgcg ggaggtctca aatcgacgtt ggataagtgc cattacgata tcctcggaaa taggattttc aggaacaaga caaacatttg cctgggcact tgccacagct gcttctgctg caataaagcc gctgtcacgg cccatgagct tcacgacgcc aacaccgtag ttgagagaaa tagcttccgc atatgcagca cgaacagcct caacagcctt gtctactgcc gtctcaaaac caaaagtgcg gtgtgagaaa ctgagatcat tatcaatagt cttgggaata ccgaaaacgg caatgttgac gccccgcctc ttggcctctt cagcaatctt aagagcacca cgttgtgtgc catcaccacc aacagtaaag aggatgttaa caccaagacg ttccagggtg tcaaccattt cactggtgtc ttggggaccg cggctgctgc ctaaaatggt tcctccgtaa cggtgaatgc ttgtgacaga tgtgcggtaa agctccatgg cggtgtgact tcccttcttt gagagacccc agtaaccaaa tcggaagcca atgacgcgct tcacgcggta tgcgttaacg gcagtcagag tcaaggaacg aatcacgtca ttcaagccgg ggcaaatacc tccgcacgtg acaatgccga caactgtttc cggtggattg aaatgaatac gctgacgggg tgcagcaagc tcacaaagga gagggcttat ggaaacggga agttccgagt gtaccccctc ctgtggacgg gggtcgtgca tgatatgttc aatcttgtcg gaaaactcct cacgcatcac atgttttttg ctggggttca tgtactttcg gccatggaga cgctccacct ttaggtcctc ctgggtgacc tcgttcagcg gggccgcgtg agaacggaca accctgctcg tgtcacgaag gcggtt
Expected Protein Sequence
MAHHHHHHME NRLRDTSRVV RSHAAPLNEV TQEDLKVERL HGRKYMNPSK KHVMREEFSD KIEHIMHDPR PQEGVHSELP VSISPLLCEL AAPRQRIHFN PPETVVGIVT CGGICPGLND VIRSLTLTAV NAYRVKRVIG FRFGYWGLSK KGSHTAMELY RTSVTSIHRY GGTILGSSRG PQDTSEMVDT LERLGVNILF TVGGDGTQRG ALKIAEEAKR RGANLAVFGI PKTIDNDLSF SHRTFGFETA VDKAVEAVRA AYAEAISLNY GVGVVKLMGR DSGFIAAEAA VASAQANICL VPENPISEDI VMALIQRRFE TSRSCVIIVA EGFGQDWEGG TGGHDASGNK KLTDIGVVLT KRIQAWLRKN KERYPNGTVK YIDPSYMIRA CPPSANDALF CATLSTLAMH EAMAGATNCI IALRYNSYIL VPIKVATSVR RVLDLRGQLW RQVREITVGL QDDVRAFKEA EVRRELEAIS LVRERLIGQL SKL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa accgccttcg tgacacgagc agggttgtcc gttctcacgc ggccccgctg aacgaggtca cccaggagga cctaaaggtg gagcgtctcc atggccgaaa gtacatgaac cccagcaaaa aacatgtgat gcgtgaggag ttttccgaca agattgaaca tatcatgcac gacccccgtc cacaggaggg ggtgcactcg gaacttcccg tttccataag ccccctcctt tgtgagcttg ctgcaccccg tcagcgtatt catttcaatc caccggaaac agttgtcggc attgtcacat gcggaggtat ttgccccggc ttgaatgacg tgattcgttc cttgactctg actgccgtta acgcataccg cgtgaagcgc gtcattggct tccgatttgg ttactggggt ctctcaaaga agggaagtca caccgccatg gagctctacc gtacatctgt cacaagcatt caccgttacg gaggaaccat tttaggcagc agccgcggtc cccaagacac cagtgaaatg gttgacaccc tggaacgtct tggtgttaac atcctcttta ctgttggtgg tgatggcaca caacgtggtg ctcttaagat tgctgaagag gccaagaggc ggggcgccaa ccttgccgtt ttcggtattc ccaagactat tgacaatgat ctcagtttct cacaccgcac ttttggtttt gagacggcag tagacaaggc tgttgaggct gtccgtgctg catatgcgga agctatttct ctcaactacg gtgttggcgt cgtgaagctc atgggccgtg acagcggctt tattgcagca gaagcagctg tggcaagtgc ccaggcaaat atttgccttg ttcctgaaaa tcctatttcc gaggatatcg taatggcact tatccaacgt cgatttgaga cctcccgcag ctgtgtcatt attgtggcag agggctttgg tcaggactgg gaagggggca ctggtggtca tgatgcctcg ggcaataaga agcttacgga tattggcgtt gtcctgacga agaggataca agcatggctg cgtaaaaata aagagcgtta ccctaatggc acagtgaaat acattgaccc ttcatacatg attcgcgcct gtccgccctc ggcaaatgac gctcttttct gcgccactct ttccacactt gccatgcacg aggctatggc tggtgcaacg aactgtatta ttgcattgcg ttacaatagc tatattctcg tgcctatcaa ggtggcaacc tcggttcgtc gtgtgttgga tctccgcggc cagctctggc gccaagtgcg cgaaatcact gttggcctgc aggatgacgt gcgtgccttc aaagaagctg aagttcgtcg tgaactggag gccatcagcc tggttcgtga gcgcctgatc ggacaactct ccaagctgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat