SSGCID: Target Page

TrcrA.00335.a

aldo/keto reductase, putative

CENTER ID:	TrcrA.00335.a
ORGANISM:	Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.00335.a.B1.GE35094	full length		1	322

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.00335.a.B1.PS01514	full length		1	322

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tc00.1047053505183.120
RefSeq:	XP_814418.1
UniProt:	Q4DJ59

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVRVIKGKGK GGGALPLLES SGGMRIPHMG VGTYELYGDE SRIAVLAALR LGFRLVDTAA GYHNEEHVGA AIEESGVQRE ELFVVVKIAP KAMASEELVE KGIRESVRKL RIAYADCVLI HWPGCGGLKP EEAEGHRAAR HRCWKVMQAL KKEGIIRHLG VSNFAPRHFP HLVGNAAEEA HLFDAANPCR PVINQIELHP LCVQQEACDF CRERDIILQQ YSPLAQGHAK LVEHPVLCAI VKDLFPGYTV QDVLLMWGLS QGFCVVVRSR SKEHLAQNWA AAKAFFDEGL LSESQKEQLK NLRESMRVVE DHHFCWHSEH ID

NT Sequence

atggtgcggg tcatcaaggg aaaaggaaaa ggcggaggtg ccctgccgct tctggagtcg agcggcggca tgcggattcc gcacatgggc gttggcacgt atgaacttta cggcgatgag tctcggattg ccgtgctggc ggctctgcgg ttggggtttc gtctcgtcga taccgccgct gggtaccaca acgaggaaca cgttggagcc gccatcgaag agagcggggt gcagcgggag gagttgtttg ttgtggtgaa gattgcccca aaggcgatgg cgtcggagga acttgtcgag aagggcattc gcgagagtgt acggaagctg cggattgcgt atgcggactg tgtgttgatt cattggcccg gctgtggtgg gctgaagccg gaggaggcgg agggacatcg agcggcacga caccgctgct ggaaggtgat gcaggccctg aagaaggagg gcatcatacg acatttgggg gtgtccaatt ttgccccgcg gcactttccc catttggttg gcaatgcggc ggaagaagcg catttatttg acgctgccaa cccatgcagg cccgtgatca atcagattga actgcacccg ctctgtgtgc agcaagaagc ttgcgacttc tgccgggaac gtgatatcat tctccagcaa tattcaccat tggcgcaggg acatgcaaaa cttgttgagc atcccgtcct gtgtgcgatc gtaaaggacc tgttccctgg gtacacagtg caagatgtgt tgctcatgtg gggcctcagt cagggcttct gtgttgtggt gcgcagtcgt tccaaggaac atttagcgca aaattgggct gccgcaaagg ctttttttga tgaagggctg ctcagtgagt cacaaaagga gcaactgaaa aacctgcgag agtcgatgcg ggttgtggag gatcatcatt tctgttggca cagtgaacat atcgactaa

Details for TrcrA.00335.a.B1.GE35094

HARVESTED ON:	4/10/2012
SEQUENCED ON:	4/11/2012
EXPECTED MW:	36kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	98
PERCENT COVERAGE:	97

Validated AA Sequence

MVRVIKEEGK DGGALPLLES SGGMRIPHMG IGTYELYGDE SRIAVLAALR LGFRLVDTAA GYHNEEHVGA AIEESGVQRE ELFVVVKIAP KAMASEELVE KGIRESVRKL RIAYADCVLI HWPGCGGLKP EEAEGHRAAR HRCWKVMQAL KKEGIIRHLG VSNFAPRHFP HLVGNASEEA HLFDAANPCR PVINQIELHP LCVQQEVCDF CRERDIILQQ YSPLAQGHAK LVEHPVLCAI VKDLFPGYTV QDVLLMWGLS QGFCVVVRSR SKEHLAQNWA AAKAFFDEGL LSESQKEQLK NLRESMRVVE DHHFCWHSEH ID

Validated NT Sequence

tactcactta gtcgatatgt tcactgtgcc aacagaaatg atgatcctcc acaacccgca tcgactctcg caggtttttc agttgctcct tttgtgactc actgagcagc ccttcatcaa aaaaggcctt tgcggcagcc caattttgcg ctaaatgttc cttggaacga ctgcgcacca caacacagaa gccctgactg aggccccaca tgagcaacac atcttgcact gtgtaaccag ggaacaggtc ctttacgatc gcacacagga cgggatgctc aacaagtttt gcatgtccct gcgccaatgg agaatattgc tggagaatga tatcacgttc ccggcagaag tcgcaaactt cttgctgcac acagagcggg tgcagttcaa tctgattgat cacgggcctg catgggttgg cagcgtcaaa taaatgcgct tcctccgaag cattgccaac caaatgggga aagtgtcgcg gggcaaaatt ggacaccccc aaatgtcgta tgatgccctc cttcttcagg gcctgcatca ccttccagca gcggtgtcgt gccgctcgat gtccctccgc ctcctccggc ttcagtccac cacagccggg ccaatgaatc aacacacagt ccgcatacgc aatccgcagc ttccgtacac tctcgcgaat gcccttctcg acaagttcct ccgacgccat cgcctttggg gcaatcttca ccacaacaaa caactcctcc cgctgcaccc cgctctcttc gatggcggct ccaacgtgtt cctcgttgtg gtacccagcg gcggtatcga cgagacgaaa ccccaaccgc agagccgcca gcacggcaat ccgagactca tcgccgtaaa gttcatacgt gccaatgccc atgtgcggaa tccgcatgcc gccgctcgac tccagaagcg gcagggcacc tccgtctttt ccttcttcct tgatgacccg caccatatgg tggtggtgg

Expected Protein Sequence

MAHHHHHHMV RVIKGKGKGG GALPLLESSG GMRIPHMGVG TYELYGDESR IAVLAALRLG FRLVDTAAGY HNEEHVGAAI EESGVQREEL FVVVKIAPKA MASEELVEKG IRESVRKLRI AYADCVLIHW PGCGGLKPEE AEGHRAARHR CWKVMQALKK EGIIRHLGVS NFAPRHFPHL VGNAAEEAHL FDAANPCRPV INQIELHPLC VQQEACDFCR ERDIILQQYS PLAQGHAKLV EHPVLCAIVK DLFPGYTVQD VLLMWGLSQG FCVVVRSRSK EHLAQNWAAA KAFFDEGLLS ESQKEQLKNL RESMRVVEDH HFCWHSEHID

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgc gggtcatcaa gggaaaagga aaaggcggag gtgccctgcc gcttctggag tcgagcggcg gcatgcggat tccgcacatg ggcgttggca cgtatgaact ttacggcgat gagtctcgga ttgccgtgct ggcggctctg cggttggggt ttcgtctcgt cgataccgcc gctgggtacc acaacgagga acacgttgga gccgccatcg aagagagcgg ggtgcagcgg gaggagttgt ttgttgtggt gaagattgcc ccaaaggcga tggcgtcgga ggaacttgtc gagaagggca ttcgcgagag tgtacggaag ctgcggattg cgtatgcgga ctgtgtgttg attcattggc ccggctgtgg tgggctgaag ccggaggagg cggagggaca tcgagcggca cgacaccgct gctggaaggt gatgcaggcc ctgaagaagg agggcatcat acgacatttg ggggtgtcca attttgcccc gcggcacttt ccccatttgg ttggcaatgc ggcggaagaa gcgcatttat ttgacgctgc caacccatgc aggcccgtga tcaatcagat tgaactgcac ccgctctgtg tgcagcaaga agcttgcgac ttctgccggg aacgtgatat cattctccag caatattcac cattggcgca gggacatgca aaacttgttg agcatcccgt cctgtgtgcg atcgtaaagg acctgttccc tgggtacaca gtgcaagatg tgttgctcat gtggggcctc agtcagggct tctgtgttgt ggtgcgcagt cgttccaagg aacatttagc gcaaaattgg gctgccgcaa aggctttttt tgatgaaggg ctgctcagtg agtcacaaaa ggagcaactg aaaaacctgc gagagtcgat gcgggttgtg gaggatcatc atttctgttg gcacagtgaa catatcgact gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for TrcrA.00335.a.B1.PS01514

PURIFICATION DATe:	5/31/2012
CONCENTRATION:	53.05mg/ml
OBSERVED MW:	34kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGTGC GGGTCATCAA GGGAAAAGGA AAAGGCGGAG GTGCCCTGCC GCTTCTGGAG TCGAGCGGCG GCATGCGGAT TCCGCACATG GGCGTTGGCA CGTATGAACT TTACGGCGAT GAGTCTCGGA TTGCCGTGCT GGCGGCTCTG CGGTTGGGGT TTCGTCTCGT CGATACCGCC GCTGGGTACC ACAACGAGGA ACACGTTGGA GCCGCCATCG AAGAGAGCGG GGTGCAGCGG GAGGAGTTGT TTGTTGTGGT GAAGATTGCC CCAAAGGCGA TGGCGTCGGA GGAACTTGTC GAGAAGGGCA TTCGCGAGAG TGTACGGAAG CTGCGGATTG CGTATGCGGA CTGTGTGTTG ATTCATTGGC CCGGCTGTGG TGGGCTGAAG CCGGAGGAGG CGGAGGGACA TCGAGCGGCA CGACACCGCT GCTGGAAGGT GATGCAGGCC CTGAAGAAGG AGGGCATCAT ACGACATTTG GGGGTGTCCA ATTTTGCCCC GCGGCACTTT CCCCATTTGG TTGGCAATGC GGCGGAAGAA GCGCATTTAT TTGACGCTGC CAACCCATGC AGGCCCGTGA TCAATCAGAT TGAACTGCAC CCGCTCTGTG TGCAGCAAGA AGCTTGCGAC TTCTGCCGGG AACGTGATAT CATTCTCCAG CAATATTCAC CATTGGCGCA GGGACATGCA AAACTTGTTG AGCATCCCGT CCTGTGTGCG ATCGTAAAGG ACCTGTTCCC TGGGTACACA GTGCAAGATG TGTTGCTCAT GTGGGGCCTC AGTCAGGGCT TCTGTGTTGT GGTGCGCAGT CGTTCCAAGG AACATTTAGC GCAAAATTGG GCTGCCGCAA AGGCTTTTTT TGATGAAGGG CTGCTCAGTG AGTCACAAAA GGAGCAACTG AAAAACCTGC GAGAGTCGAT GCGGGTTGTG GAGGATCATC ATTTCTGTTG GCACAGTGAA CATATCGACT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT