TrcrA.00255.b

protein kinase, putative,cdc2-related related kinase, putative

CENTER ID: TrcrA.00255.b
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.00255.b.B1.GE35093 full length 1 339
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053508325.320
RefSeq: XP_820138.1
UniProt: Q4E0H6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIFGCGGILA SPESILFVPF IFSRCRRMEE RGEEEKKRRE RWSLDMDRFI IGPPIGEGRF GVVCAAVAKE SGMPAAIKFV TTPRLDEGIP HPTARELLVA MRLVHPFVIR TYEVVPCGCS LALVMERCKT DLAAVLSGRT AGNPLPALEA KSLFKMLLLA LAYIHSEGVL HRDVKPLNCF LTAEGELRLG DFGLSRVWDT EASMTHEVVS RWYRAPELLL GQRHYGPEID VWSSGCVFAE LLRGHSGALF AGDGDIWQLS RIFDVLGTPT PTSWPSAVLL PDWGKVHFEP KRPLPLREYF PDASECALDL LRRLLCLDPQ ERLTAAGALR HAYFSEYPT
NT Sequence
atgatctttg ggtgcggagg gattttggca tcaccggagt caattctttt tgttccattt atattttcac gatgccgtag aatggaggag aggggtgaag aagagaaaaa aagaagagag aggtggtcgc ttgacatgga tcgttttatt attggacccc caattggtga gggccgcttt ggcgtcgttt gtgctgcagt tgctaaggaa tctggaatgc cagccgcaat caaattcgtc acaactccac gtctcgatga gggtattccg catcccacag cgagggaact acttgttgca atgcgattgg tgcatccttt tgttattcgg acatatgagg tagttccgtg tggctgctcc ttggcgttag tgatggagcg ttgcaagact gacttggcag cagtcctctc agggagaact gccggcaatc ctctgcccgc gttagaggca aagtctttat ttaaaatgct tcttttggcg ttggcgtaca tacacagcga aggtgttctt catcgagatg tgaaaccttt aaattgtttc ttgactgcgg agggggaact acgcctcggc gactttggac tcagtcgtgt atgggatacg gaagcatcca tgactcacga agtcgtttcg aggtggtacc gagctcccga gttacttctg ggccagcgac actacggacc cgaaattgat gtgtggtcat cagggtgcgt atttgcggaa cttctacggg ggcacagtgg tgctcttttt gcaggcgatg gagatatttg gcagttgtca cgaatatttg atgtattagg gacaccgacg cccacatcgt ggccatcggc ggtgctgctt cccgattggg gaaaggtgca ctttgagccg aagcgtcctc ttcctctccg tgaatatttc ccggatgcct ctgaatgtgc attggatttg ttgcgaagat tactttgcct ggatcctcaa gagcgtttga ctgcggctgg cgcccttcgt cacgcctact ttagtgaata cccgacgtga
Details for TrcrA.00255.b.B1.GE35093
HARVESTED ON: 4/10/2012
SEQUENCED ON: 4/11/2012
EXPECTED MW: 38kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 58
PERCENT COVERAGE: 39
Validated AA Sequence
MAHHHHHHMI FGXGGILASP ESNFCVXFIF XXCXXXEXRG EXEXKXXERW SLDXDRFIIX SSIXEGXFGV VCAAXXXXSG MPXAXXFXTT PRLDEGIPHP SEGTTCCXAI GASFCYSDIX XXSVWXLLGX TXXTLXD
Validated NT Sequence
tttnnnnnnn ttaanaagga gatataccat ggctcaccac caccaccacc atatgatctt tggntgngga gggattttgg catcaccgga gtcaaatttt tgtgttcnnt ttatatttcn nnnatgccnt anaanggagg anaggggtga anaagagaan aaaanannag agaggtggtc gcttgacntg gatcgtttta ttattgnatc ctcaattgnt gagggcnnnt ttggcgtcgt ttgtgctgcn nttntnnnng antctggaat gccnnccgca ntcnaattcn tcacaactcc ncgtctcgat gagggtattc cgcatcccag cgaggggact acttgttgca ntgcnattgg tgcatccttt tgttattctg acatatnang tanttccgtg tggntgctcc ttggcnttac ngnngnaacg ttgcangact ganntgnnan cagtcctctc angganaact gccngnaatc ctctgcccgc gttanangcn aantctttat ttannatgct tcnnntggnn ttnncntann tncncagcnn ggntgttctt cntcgnnatg tgaaancttn nnnntttttc ntgantgcnn atggngaact nnntctcgnc nacnttggac taantnnngt ntnnnatncg gaagcntcca tgactcaaga nntcnnttcn aggtggtacc ganctgnngg gttacttctg gnnnngcnac nctacngnnc ganattgatg tgtgntcatc nnggtgcgta tttgcnnann ttctacgnng tacnntggtg ctttttttgn acgcgatnnn natnnntggn anttgtnnnn nanntttgat ntattattga cnnngnnnnc cncntcnngn cnatcccngg tgctgcttcn nnantgnnna aaangtgnnn tttnnnnnnn nnnnncnnnn nnnnnnnccn nnantttttt cccggatgcn nnnnantgng catnttnnnt gcgannnnac tttnnnnnnn cnnnnnngcg tttgantgng ntgnnnncnt nnnnnnnnnc nncnttnnng nnnncnnncg nanntnnntn nnnnnnnnnn nc
Expected Protein Sequence
MAHHHHHHMI FGCGGILASP ESILFVPFIF SRCRRMEERG EEEKKRRERW SLDMDRFIIG PPIGEGRFGV VCAAVAKESG MPAAIKFVTT PRLDEGIPHP TARELLVAMR LVHPFVIRTY EVVPCGCSLA LVMERCKTDL AAVLSGRTAG NPLPALEAKS LFKMLLLALA YIHSEGVLHR DVKPLNCFLT AEGELRLGDF GLSRVWDTEA SMTHEVVSRW YRAPELLLGQ RHYGPEIDVW SSGCVFAELL RGHSGALFAG DGDIWQLSRI FDVLGTPTPT SWPSAVLLPD WGKVHFEPKR PLPLREYFPD ASECALDLLR RLLCLDPQER LTAAGALRHA YFSEYPT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatct ttgggtgcgg agggattttg gcatcaccgg agtcaattct ttttgttcca tttatatttt cacgatgccg tagaatggag gagaggggtg aagaagagaa aaaaagaaga gagaggtggt cgcttgacat ggatcgtttt attattggac ccccaattgg tgagggccgc tttggcgtcg tttgtgctgc agttgctaag gaatctggaa tgccagccgc aatcaaattc gtcacaactc cacgtctcga tgagggtatt ccgcatccca cagcgaggga actacttgtt gcaatgcgat tggtgcatcc ttttgttatt cggacatatg aggtagttcc gtgtggctgc tccttggcgt tagtgatgga gcgttgcaag actgacttgg cagcagtcct ctcagggaga actgccggca atcctctgcc cgcgttagag gcaaagtctt tatttaaaat gcttcttttg gcgttggcgt acatacacag cgaaggtgtt cttcatcgag atgtgaaacc tttaaattgt ttcttgactg cggaggggga actacgcctc ggcgactttg gactcagtcg tgtatgggat acggaagcat ccatgactca cgaagtcgtt tcgaggtggt accgagctcc cgagttactt ctgggccagc gacactacgg acccgaaatt gatgtgtggt catcagggtg cgtatttgcg gaacttctac gggggcacag tggtgctctt tttgcaggcg atggagatat ttggcagttg tcacgaatat ttgatgtatt agggacaccg acgcccacat cgtggccatc ggcggtgctg cttcccgatt ggggaaaggt gcactttgag ccgaagcgtc ctcttcctct ccgtgaatat ttcccggatg cctctgaatg tgcattggat ttgttgcgaa gattactttg cctggatcct caagagcgtt tgactgcggc tggcgccctt cgtcacgcct actttagtga atacccgacg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat