TrcrA.00251.a

serine/threonine-protein kinase, putative

CENTER ID: TrcrA.00251.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.00251.a.B1.GE35090 full length 1 583
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053511715.20
RefSeq: XP_809553.1
UniProt: Q4D5A5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGNGAVKLRT DMHGDVPMGS LNEDDINDDA VTSVITLPKC AREQKMDGSD DMRPIPIGEG EEKVKGSYRS EPVSLDEVLG PADSVKARYR KNKAIGKGAF GEAYIVERNP LYPIPSPKKA AGGKGDSEIK KRAGDDATRL FVAKVMDLTS MSPNDRRYAQ TEIMCLACNH HFAIIQYYEH FFIDDEDETI IIITEFADGG DLYRTLHNES SEFCLSETEA GIYFVQLLLG LDHVHRRRMI HRDVKTANIF LTTRGFLKLG DFGFSQQYDS TVSNPIACTF LGTSYYLAPE MWREQRYGKK ADIWAAGIVL CELLGKRRPF EADSIPKMKE LVLSGAMWLP PVLPENAGVS EEERQAQKKM GYISREMREF LEFILQQDPE KRPSASQLLK TPLMQHYLCL LKKRVDRMIA LDDEMESNYK ANPARFAGSL PEYNISLEGR ALVMSGIAEG EAVINFETER EMATSTTPRI ESIVYKETAG SQWKERYLIL ADGFLTMTLA KGKMAAGSGE RSKKVPLQSI RAVAPVVLDV PVLAESSGES GPFHVKFAFA ISTQNAHSIV FATRTEQERD QWLSTMIVAL KMD
NT Sequence
atgggcaacg gtgccgtaaa gctgcggacg gatatgcatg gcgacgtgcc gatgggcagt ctaaatgagg atgacattaa tgatgacgcg gtaacatcag taataacgtt gccaaaatgc gccagggaac aaaagatgga cggaagtgat gacatgcgcc ctattcctat tggagaaggt gaggaaaaag tcaaaggttc atatcgttcc gaacctgttt ctttagatga agtcttggga cctgccgaca gcgtaaaggc gcggtaccga aagaataaag caattggtaa aggggcattt ggtgaggctt atattgttga gcgcaaccca ctgtatccaa ttccatcccc caaaaaagca gcgggaggga aaggcgattc cgaaataaaa aaaagagctg gtgatgatgc aacccgttta tttgtagcta aagtgatgga tttgacttca atgtcaccaa atgacagacg atacgctcaa actgaaatta tgtgtcttgc ctgtaatcat cattttgcaa tcatacagta ctatgagcac ttttttatcg atgatgagga tgagacaatt ataattataa cagagtttgc ggatgggggg gatctctacc gcaccctcca caatgagtct tcggagtttt gtctttcaga gacagaagcc ggaatttatt ttgtgcagtt gttacttggt cttgaccacg ttcatcgtcg tcgtatgatc catcgtgatg taaagacggc gaatattttc ttgaccacgc gtggtttttt gaagcttggt gattttggct tttcacagca gtacgacagc accgtctcga acccaatagc ctgcacattt cttggcacaa gctactatct cgccccagag atgtggcgcg agcagcgtta tggcaaaaaa gcagatatct gggcggctgg aatagttctg tgtgaacttt tggggaaacg gcggcctttc gaagcggact ccattcccaa aatgaaggaa cttgttctct ctggtgccat gtggcttccc cctgtacttc ctgaaaacgc cggcgtctca gaggaggagc gtcaagccca gaaaaagatg ggctacatat ccagagagat gagggagttc ctggagttca ttttgcagca ggaccctgag aaacggccaa gcgcttccca acttctcaaa acaccattga tgcagcatta tctgtgttta cttaagaaac gtgtggatcg tatgattgca ttggatgacg aaatggagag taattacaag gcaaatcccg cgaggtttgc gggttcattg cccgaatata atatttccct ggagggccgg gcactggtta tgagcggaat agcggagggc gaggcggtga tcaactttga gacggaacgg gagatggcaa caagcaccac gcctcgtatt gaaagcatag tttacaagga gacagccggt agtcaatgga aagaacgtta cctcattctc gccgatgggt ttttaacaat gacacttgca aaggggaaaa tggcggcggg ctctggcgaa cgcagtaaga aagtcccgct ccaatcaatt agggccgtcg caccagttgt tttggatgtt ccagtcttag ctgaatcgag cggggaatct ggtccgtttc acgtaaagtt tgcctttgcc atatctacac aaaatgcgca ttctattgta tttgcaaccc ggaccgagca ggaacgtgat cagtggctgt ctacgatgat tgtagcgttg aaaatggatt ag
Details for TrcrA.00251.a.B1.GE35090
HARVESTED ON: 4/10/2012
SEQUENCED ON: 4/11/2012
EXPECTED MW: 66kDa
OBSERVED MW: 66kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 43
Validated AA Sequence
MKELVLSGAM WLPPVLPENA GVSEEERQAQ KKMGYISREM REFLEFILQQ DPEKRPSASQ LLKTPLMQHY LCLLKKRVDR MIALDDEMES NYKANPARFA GSLPEYNISL EGRALVMSGI AEGEAVINFE TEREMATSTT PRIESIVYKE TAGSQWKERY LILADGFLTM TLAKGKMAAG SGERSKKVPL QSIRAVAPVV LDVPVLAESS GESGPFHVKF AFAISTQNAH SIVFATRTEQ ERDQWLSTMI VALKMD
Validated NT Sequence
cnttcgngct ttgttagcag ccggatccta tcttactcac ttaatccatt ttcaacgcta caatcatcgt agacagccac tgatcacgtt cctgctcggt ccgggttgca aatacaatag aatgcgcatt ttgtgtagat atggcaaagg caaactttac gtgaaacgga ccagattccc cgctcgattc agctaagact ggaacatcca aaacaactgg tgcgacggcc ctaattgatt ggagcgggac tttcttactg cgttcgccag agcccgccgc cattttcccc tttgcaagtg tcattgttaa aaacccatcg gcgagaatga ggtaacgttc tttccattga ctaccggctg tctccttgta aactatgctt tcaatacgag gcgtggtgct tgttgccatc tcccgttccg tctcaaagtt gatcaccgcc tcgccctccg ctattccgct cataaccagt gcccggccct ccagggaaat attatattcg ggcaatgaac ccgcaaacct cgcgggattt gccttgtaat tactctccat ttcgtcatcc aatgcaatca tacgatccac acgtttctta agtaaacaca gataatgctg catcaatggt gttttgagaa gttgggaagc gcttggccgt ttctcagggt cctgctgcaa aatgaactcc aggaactccc tcatctctct ggatatgtag cccatctttt tctgggcttg acgctcctcc tctgagacgc cggcgttttc aggaagtaca gggggaagcc acatggcacc agagagaaca agttccttca ttttgggaat ggagtccgct tcgaaaggcg ccgtttcccc aaaagttcac acagaactat tccagccgcc cagatatctg cttttttgcc atancgctgc tcgcgccaca tctctggggc gagatagtag cttgngcnan nntgngcnng ctantnnntt cnagacggtg ctgtcgtact gctgtgaaag ncaaatcanc nagcttcnaa aannnnncnt nnnannnaat ntnnncgtcn ttnnnncnnn ngntcnnncn acnnnnnnna nngnnacnnn ananngcnnn aannaannnc nnntnnngnn nntnaannan nannncnnnn annnnnnnnn nnnnnnnnnn annnnccccn tnnnnnnann nnnnnnnnnn nnnnna
Expected Protein Sequence
MAHHHHHHMG NGAVKLRTDM HGDVPMGSLN EDDINDDAVT SVITLPKCAR EQKMDGSDDM RPIPIGEGEE KVKGSYRSEP VSLDEVLGPA DSVKARYRKN KAIGKGAFGE AYIVERNPLY PIPSPKKAAG GKGDSEIKKR AGDDATRLFV AKVMDLTSMS PNDRRYAQTE IMCLACNHHF AIIQYYEHFF IDDEDETIII ITEFADGGDL YRTLHNESSE FCLSETEAGI YFVQLLLGLD HVHRRRMIHR DVKTANIFLT TRGFLKLGDF GFSQQYDSTV SNPIACTFLG TSYYLAPEMW REQRYGKKAD IWAAGIVLCE LLGKRRPFEA DSIPKMKELV LSGAMWLPPV LPENAGVSEE ERQAQKKMGY ISREMREFLE FILQQDPEKR PSASQLLKTP LMQHYLCLLK KRVDRMIALD DEMESNYKAN PARFAGSLPE YNISLEGRAL VMSGIAEGEA VINFETEREM ATSTTPRIES IVYKETAGSQ WKERYLILAD GFLTMTLAKG KMAAGSGERS KKVPLQSIRA VAPVVLDVPV LAESSGESGP FHVKFAFAIS TQNAHSIVFA TRTEQERDQW LSTMIVALKM D
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggca acggtgccgt aaagctgcgg acggatatgc atggcgacgt gccgatgggc agtctaaatg aggatgacat taatgatgac gcggtaacat cagtaataac gttgccaaaa tgcgccaggg aacaaaagat ggacggaagt gatgacatgc gccctattcc tattggagaa ggtgaggaaa aagtcaaagg ttcatatcgt tccgaacctg tttctttaga tgaagtcttg ggacctgccg acagcgtaaa ggcgcggtac cgaaagaata aagcaattgg taaaggggca tttggtgagg cttatattgt tgagcgcaac ccactgtatc caattccatc ccccaaaaaa gcagcgggag ggaaaggcga ttccgaaata aaaaaaagag ctggtgatga tgcaacccgt ttatttgtag ctaaagtgat ggatttgact tcaatgtcac caaatgacag acgatacgct caaactgaaa ttatgtgtct tgcctgtaat catcattttg caatcataca gtactatgag cactttttta tcgatgatga ggatgagaca attataatta taacagagtt tgcggatggg ggggatctct accgcaccct ccacaatgag tcttcggagt tttgtctttc agagacagaa gccggaattt attttgtgca gttgttactt ggtcttgacc acgttcatcg tcgtcgtatg atccatcgtg atgtaaagac ggcgaatatt ttcttgacca cgcgtggttt tttgaagctt ggtgattttg gcttttcaca gcagtacgac agcaccgtct cgaacccaat agcctgcaca tttcttggca caagctacta tctcgcccca gagatgtggc gcgagcagcg ttatggcaaa aaagcagata tctgggcggc tggaatagtt ctgtgtgaac ttttggggaa acggcggcct ttcgaagcgg actccattcc caaaatgaag gaacttgttc tctctggtgc catgtggctt ccccctgtac ttcctgaaaa cgccggcgtc tcagaggagg agcgtcaagc ccagaaaaag atgggctaca tatccagaga gatgagggag ttcctggagt tcattttgca gcaggaccct gagaaacggc caagcgcttc ccaacttctc aaaacaccat tgatgcagca ttatctgtgt ttacttaaga aacgtgtgga tcgtatgatt gcattggatg acgaaatgga gagtaattac aaggcaaatc ccgcgaggtt tgcgggttca ttgcccgaat ataatatttc cctggagggc cgggcactgg ttatgagcgg aatagcggag ggcgaggcgg tgatcaactt tgagacggaa cgggagatgg caacaagcac cacgcctcgt attgaaagca tagtttacaa ggagacagcc ggtagtcaat ggaaagaacg ttacctcatt ctcgccgatg ggtttttaac aatgacactt gcaaagggga aaatggcggc gggctctggc gaacgcagta agaaagtccc gctccaatca attagggccg tcgcaccagt tgttttggat gttccagtct tagctgaatc gagcggggaa tctggtccgt ttcacgtaaa gtttgccttt gccatatcta cacaaaatgc gcattctatt gtatttgcaa cccggaccga gcaggaacgt gatcagtggc tgtctacgat gattgtagcg ttgaaaatgg attgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat