TrcrA.00198.b
iron superoxide dismutase, putative
| CENTER ID: | TrcrA.00198.b |
| ORGANISM: | Trypanosoma cruzi |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| TrcrA.00198.b.B1.GE35089 | full length | 1 | 233 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| TrcrA.00198.b.B1.PS01521 | full length | 1 | 233 |
Structures
4H3E
DEPOSITED: 9/13/2012
DETERMINATION: XRay
CLONE: TrcrA.00198.b.B1.GE35089
PROTEIN: TrcrA.00198.b.B1.PS01521
Publications by SSGCID
Iron superoxide dismutases in eukaryotic pathogens: new insights from Apicomplexa and Trypanosoma structures.
Anupama A, Cestari I, Davies DR, Edwards TE, Fairman JW, Moretti NS, Myler PJ, Phan IQ, Schenkman S, Shanmugam D, Stuart K
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2015
volume 71, issue Pt 5, pages 615-21
PMID: 25961325; PMCID: PMC4427173
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | TritrypDB:Tc00.1047053509775.40 |
| OrthoMCL: | OG5_126676 |
| RefSeq: | XP_812157.1 |
| UniProt: | Q4DCQ3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRRAVNISI ARGRMALMSY ATLPDLLKPS GAPAELPKLG FNWKDGCAPV FSPRQMELHY TKHHKAYVDK LNALAGTTYD GKSIEEIILA VANDAEKKGL FNQAAQHFNH TFYFRCITPN GKAMPKSLES AVTAQFGSVE QFKDAFVQAG VNNFGSGWTW LCVDPSNKNQ LVIDNTSNAG CPLTKGLRPV LAVDVWEHAY YKDFENRRPD YLKEIWSVID WEFVAKMHAQ AIK
NT Sequence
atgttgagac gtgcggtgaa tatatccatt gccagagggc gcatggcgtt gatgagctac gcgacgctgc ctgacctttt aaaaccaagt ggcgccccgg ccgagttgcc caagttgggg tttaactgga aggatggatg tgcccctgtc ttcagcccgc gccagatgga attgcactac accaaacacc acaaggcgta tgtggacaaa ttaaatgcgc tggcagggac aacatacgat gggaagagca ttgaggagat tatccttgcg gtcgcaaatg acgccgagaa gaagggtctt ttcaaccaag ccgcacagca ttttaaccac accttttact tccgttgcat cacccctaat gggaaggcga tgccaaagtc ccttgagtct gctgttacgg cccagtttgg ttcggtggaa cagtttaaag atgcctttgt gcaggcaggg gtgaataact ttggctcggg ttggacgtgg ctgtgcgtgg acccgtcgaa taagaatcag ctcgttattg acaatacgag caacgccgga tgtcccttaa cgaagggact gcgccccgtc ctcgcagtgg atgtgtggga gcatgcgtac tacaaggact ttgaaaaccg ccggccggat tacttgaagg agatctggtc ggtcatcgac tgggagtttg ttgcaaagat gcatgcgcag gcaataaaat aa
Details for TrcrA.00198.b.B1.GE35089
| HARVESTED ON: | 4/10/2012 |
| SEQUENCED ON: | 4/11/2012 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHML RRAVNISIAR GRMALMSYAT LPDLLKPSGA PAELPKLGFN WKDGCAPVFS PRQMELHYTK HHKAYVDKLN ALAGTTYDGK SIEEIILAVA NDAEKKGLFN QAAQHFNHTF YFRCITPNGK AMPKSFESAV TAQFGSVEQF KDAFVQAGVN NFGSGWTWLC VDPSNKNQLV IDNTSNAGCP LTKGLRPVLA VDVWEHAYYK DFENRRPDYL KEIWSVIDWE FVAKMHAQAI K
Validated NT Sequence
nnnnnnnnnn nnnnnnnnnn ntttgtttac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttga gacgtgcggt gaatatatcc attgccagag ggcgcatggc gttgatgagc tacgcgacgc tgcctgacct tttaaaacca agtggcgccc cggccgagtt gcccaagtta gggtttaact ggaaggatgg atgtgcccct gtcttcagcc cgcgccagat ggaattgcac tacaccaaac accacaaggc gtatgtggac aaattaaatg cgctggcagg gacaacatac gatgggaaga gcattgagga gattatcctt gcggtcgcaa atgacgccga gaagaagggt cttttcaacc aagccgcaca gcattttaac cacacctttt acttccgttg catcacccct aatgggaagg cgatgccaaa gtcctttgag tctgctgtta cggcccagtt tggttcggtg gaacagttta aagatgcctt tgtgcaggca ggggtgaata actttggctc gggttggacg tggctgtgcg tggacccgtc gaataagaat cagctcgtta ttgacaatac gagcaacgcc ggatgcccct taacgaaggg actgcgcccc gtcctcgcag tggatgtgtg ggagcatgcg tactacaagg actttgaaaa ccgccggccg gattacttga aggagatctg gtcggtcatc gactgggagt ttgttgcaaa gatgcatgcg caggcaataa aataagtgag taagatagga tccggnnnnn nnnncccccc naannnnnnn nngnnnnnnn nnnnnnnnnn ncnnnnggnn nnnnngnnnn ntnnnnnnng ntcanccnnc annnnnnnnn annnnnnn
Expected Protein Sequence
MAHHHHHHML RRAVNISIAR GRMALMSYAT LPDLLKPSGA PAELPKLGFN WKDGCAPVFS PRQMELHYTK HHKAYVDKLN ALAGTTYDGK SIEEIILAVA NDAEKKGLFN QAAQHFNHTF YFRCITPNGK AMPKSLESAV TAQFGSVEQF KDAFVQAGVN NFGSGWTWLC VDPSNKNQLV IDNTSNAGCP LTKGLRPVLA VDVWEHAYYK DFENRRPDYL KEIWSVIDWE FVAKMHAQAI K
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttga gacgtgcggt gaatatatcc attgccagag ggcgcatggc gttgatgagc tacgcgacgc tgcctgacct tttaaaacca agtggcgccc cggccgagtt gcccaagttg gggtttaact ggaaggatgg atgtgcccct gtcttcagcc cgcgccagat ggaattgcac tacaccaaac accacaaggc gtatgtggac aaattaaatg cgctggcagg gacaacatac gatgggaaga gcattgagga gattatcctt gcggtcgcaa atgacgccga gaagaagggt cttttcaacc aagccgcaca gcattttaac cacacctttt acttccgttg catcacccct aatgggaagg cgatgccaaa gtcccttgag tctgctgtta cggcccagtt tggttcggtg gaacagttta aagatgcctt tgtgcaggca ggggtgaata actttggctc gggttggacg tggctgtgcg tggacccgtc gaataagaat cagctcgtta ttgacaatac gagcaacgcc ggatgtccct taacgaaggg actgcgcccc gtcctcgcag tggatgtgtg ggagcatgcg tactacaagg actttgaaaa ccgccggccg gattacttga aggagatctg gtcggtcatc gactgggagt ttgttgcaaa gatgcatgcg caggcaataa aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for TrcrA.00198.b.B1.PS01521
| PURIFICATION DATe: | 6/11/2012 |
| CONCENTRATION: | 34.4mg/ml |
| OBSERVED MW: | 25kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML RRAVNISIAR GRMALMSYAT LPDLLKPSGA PAELPKLGFN WKDGCAPVFS PRQMELHYTK HHKAYVDKLN ALAGTTYDGK SIEEIILAVA NDAEKKGLFN QAAQHFNHTF YFRCITPNGK AMPKSFESAV TAQFGSVEQF KDAFVQAGVN NFGSGWTWLC VDPSNKNQLV IDNTSNAGCP LTKGLRPVLA VDVWEHAYYK DFENRRPDYL KEIWSVIDWE FVAKMHAQAI K
Validated NT Sequence
nnnnnnnnnn nnnnnnnnnn ntttgtttac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttga gacgtgcggt gaatatatcc attgccagag ggcgcatggc gttgatgagc tacgcgacgc tgcctgacct tttaaaacca agtggcgccc cggccgagtt gcccaagtta gggtttaact ggaaggatgg atgtgcccct gtcttcagcc cgcgccagat ggaattgcac tacaccaaac accacaaggc gtatgtggac aaattaaatg cgctggcagg gacaacatac gatgggaaga gcattgagga gattatcctt gcggtcgcaa atgacgccga gaagaagggt cttttcaacc aagccgcaca gcattttaac cacacctttt acttccgttg catcacccct aatgggaagg cgatgccaaa gtcctttgag tctgctgtta cggcccagtt tggttcggtg gaacagttta aagatgcctt tgtgcaggca ggggtgaata actttggctc gggttggacg tggctgtgcg tggacccgtc gaataagaat cagctcgtta ttgacaatac gagcaacgcc ggatgcccct taacgaaggg actgcgcccc gtcctcgcag tggatgtgtg ggagcatgcg tactacaagg actttgaaaa ccgccggccg gattacttga aggagatctg gtcggtcatc gactgggagt ttgttgcaaa gatgcatgcg caggcaataa aataagtgag taagatagga tccggnnnnn nnnncccccc naannnnnnn nngnnnnnnn nnnnnnnnnn ncnnnnggnn nnnnngnnnn ntnnnnnnng ntcanccnnc annnnnnnnn annnnnnn
Expressed Protein Sequence
MAHHHHHHML RRAVNISIAR GRMALMSYAT LPDLLKPSGA PAELPKLGFN WKDGCAPVFS PRQMELHYTK HHKAYVDKLN ALAGTTYDGK SIEEIILAVA NDAEKKGLFN QAAQHFNHTF YFRCITPNGK AMPKSLESAV TAQFGSVEQF KDAFVQAGVN NFGSGWTWLC VDPSNKNQLV IDNTSNAGCP LTKGLRPVLA VDVWEHAYYK DFENRRPDYL KEIWSVIDWE FVAKMHAQAI K
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTGA GACGTGCGGT GAATATATCC ATTGCCAGAG GGCGCATGGC GTTGATGAGC TACGCGACGC TGCCTGACCT TTTAAAACCA AGTGGCGCCC CGGCCGAGTT GCCCAAGTTG GGGTTTAACT GGAAGGATGG ATGTGCCCCT GTCTTCAGCC CGCGCCAGAT GGAATTGCAC TACACCAAAC ACCACAAGGC GTATGTGGAC AAATTAAATG CGCTGGCAGG GACAACATAC GATGGGAAGA GCATTGAGGA GATTATCCTT GCGGTCGCAA ATGACGCCGA GAAGAAGGGT CTTTTCAACC AAGCCGCACA GCATTTTAAC CACACCTTTT ACTTCCGTTG CATCACCCCT AATGGGAAGG CGATGCCAAA GTCCCTTGAG TCTGCTGTTA CGGCCCAGTT TGGTTCGGTG GAACAGTTTA AAGATGCCTT TGTGCAGGCA GGGGTGAATA ACTTTGGCTC GGGTTGGACG TGGCTGTGCG TGGACCCGTC GAATAAGAAT CAGCTCGTTA TTGACAATAC GAGCAACGCC GGATGTCCCT TAACGAAGGG ACTGCGCCCC GTCCTCGCAG TGGATGTGTG GGAGCATGCG TACTACAAGG ACTTTGAAAA CCGCCGGCCG GATTACTTGA AGGAGATCTG GTCGGTCATC GACTGGGAGT TTGTTGCAAA GATGCATGCG CAGGCAATAA AATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT