TrcrA.00174.a

serine/threonine protein phosphatase, putative

CENTER ID: TrcrA.00174.a
ORGANISM: Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrA.00174.a.B1.GE35087 full length 1 418
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tc00.1047053509029.10
RefSeq: XP_815100.1
UniProt: Q4DL63

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
NKRSISLDPD ALVSHHTLRT FHKQLLDGCG VLSPLSSYGN AIYHYIVKTF AVRNGCRHSS SDIEGFGRTL CSLCGEVKAV LQREPRHGSV VSPCYVFGDL HGNFLDLFYF LDNLISFQDL RYTPHRFLFL GDYVDRGEFS VEVLAYLFAM KVLAPEKVLL LRGNHEDTLV SGDISGYGNN SFRAQCQNLF GHALGEELWH RASETFAYLP LTANIDGRIF CTHGGIPRYR GGVDDRLHIL SRSDFPAFES FFQVPEEDTA YMRMCRQMAM DTCWADPAED ESTLDKWGFG ANPRGSGVIV FGSVAVDHFL LNNNFEYIFR AHQEKSDGLK LSKSARVFTI FSTSAYVGHE NGAGVVLVAD GKIRLIIKSA DDTARTPVVA EEEEDKDEEE EEKEAEIEAE EREEKKNEKM RKSSRLVR
NT Sequence
aataaacgct ccatttcgtt agatcccgat gcgctcgtgt cacaccacac gcttcgcacg ttccataagc aattactgga tggctgtggg gtcttatccc ctctctcctc ctacggcaat gctatttacc actacattgt gaagacgttt gcggttcgca atggctgtcg tcatagctcc tccgacattg agggttttgg ccgcacatta tgtagcctct gtggggaggt gaaggccgtc ctacagaggg aaccacgaca tggaagtgtt gtttccccat gttatgtctt tggcgaccta catggtaact ttttggatct attctacttt ctcgataatc ttatctcgtt tcaggatctg cgttacaccc cgcatcgctt tctttttctt ggtgactacg tggatcgcgg ggagtttagt gtggaggtgt tggcatacct cttcgccatg aaagtgctgg caccagagaa ggtgctgctg ctgcgtggca atcacgagga cacactcgtc agtggggaca tctccggata tggcaacaac agctttcgag cgcaatgtca aaaccttttt ggccacgctc ttggtgagga actgtggcac cgtgcaagtg agacatttgc ctatctccca ctcactgcaa acattgatgg gcgcatcttc tgcacccatg gcggaatccc acgctaccgc ggtggtgtag atgacaggct gcatatatta agccggagcg attttcctgc atttgagtct ttctttcaag ttcccgaaga ggatacggca tacatgcgta tgtgtcggca gatggccatg gacacatgtt gggccgatcc cgctgaggat gagagcacac ttgacaagtg gggttttggt gccaatccgc gcggttctgg cgttattgtc tttggctccg ttgccgtgga tcattttctt ttaaataata actttgaata catcttccgt gcccaccaag agaagtcaga tgggctgaaa ctcagtaaga gtgcccgggt ctttacaata tttagcacca gcgcgtatgt tgggcacgaa aacggtgccg gtgttgtact tgtggcggat gggaagattc gactgattat taaaagcgcc gatgacaccg ctcgtacacc agtagtagcg gaggaagagg aagacaagga cgaagaagaa gaggaaaaag aggctgagat tgaagcggag gagagggagg agaaaaagaa tgaaaaaatg cgaaaatcat cacgtctggt gagataa
Details for TrcrA.00174.a.B1.GE35087
HARVESTED ON: 4/10/2012
SEQUENCED ON: 4/11/2012
EXPECTED MW: 48kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 97
PERCENT COVERAGE: 74
Validated AA Sequence
MAHHHHHHMN KRSISLDPDA LVSHHTLRTF HKQLLDGCGV LSPLSSYGNA IYHYIVKTFA VRNGCRHSSS DIEGFGRTLC SLCGEVKAVL QREPRHGSVV SPCYVFGDLH GNFLDLFYFL DNLISFQDLR YTPHRFLFLG DYVDRGEFSV EVLAYLFAMK VLAPEKVLLL RGNHEDTLVS GDISGYGNNS FRAQCQNLFG HALGEELWHR ASETFAYLPL TANIDGRIFC THGGIPRYRG GVDDRLHILS RXDFPAFESF FXVPEXDTAY MRMCRQMAMD TCWADPAEDE STLDKWGFGA NPRGXGVIVF GSVAVXIFF
Validated NT Sequence
annnttttgt ttnctttaag aaggagatat accatggctc accaccacca ccaccatatg aataaacgct ccatttcgtt agatcccgat gcgctcgtgt cacaccacac gcttcgcacg ttccataagc aattactgga tggctgtggg gtcttatccc ctctctcctc ctacggcaat gctatttacc actacattgt gaagacgttt gcggttcgca atggctgtcg tcatagctcc tccgacattg agggttttgg ccgcacatta tgtagcctct gtggggaggt gaaggccgtc ctacagaggg aaccacgaca tggaagtgtt gtttccccat gttatgtctt tggcgaccta catggtaact ttttggatct attctacttt ctcgataatc ttatctcgtt tcaggatctg cgttacaccc cgcatcgctt tctttttctt ggtgactacg tggatcgcgg ggagtttagt gtggaggtgt tggcatacct cttcgccatg aaagtgctgg caccagagaa ggtgctgctg ctgcgtggca atcacgagga cacactcgtc agtggggaca tctccggata tggcaacaac agctttcgag cgcaatgtca aaaccttttt ggccacgctc ttggtgagga actgtggcac cgtgcaagtg agacatttgc ctatctccca ctcactgcaa acattgatgg gcgcatcttc tgcacccatg gcggaatccc acgctaccgc ggtggtgtag atgacaggct gcatatatta agccggancg attttcctgc atttgagtct ttctttcnag ttcccgaaga ngatacngca tacatgcgta tgtgtcggca gatggccatg gacacatgtt gggccgatcc cgctgaggat gagagcacac ttgacaagtg gggttttggt gccaatccgc gcggnnctgg cgttattgtc tttggctccg ttgccgtgnn cattttcttt taaatnanan tttgaatacn tcntncgngc cnnccanana ntcangggnt gaaactcagt anannnncnn ggncnttann atnnttagcn ncnnnngtan gntnggcnnn aaannnnnnn nnnnannnng nnnnnggncn actgannntn nannnnnnan nnnnnnnnnn nnnnnnnnnn nnnnngnnnn nnnnnnnnnn naannnnnan nngntnnnnn nnnnnnnnng nnnnaaaann nnnnnaa
Expected Protein Sequence
MAHHHHHHMN KRSISLDPDA LVSHHTLRTF HKQLLDGCGV LSPLSSYGNA IYHYIVKTFA VRNGCRHSSS DIEGFGRTLC SLCGEVKAVL QREPRHGSVV SPCYVFGDLH GNFLDLFYFL DNLISFQDLR YTPHRFLFLG DYVDRGEFSV EVLAYLFAMK VLAPEKVLLL RGNHEDTLVS GDISGYGNNS FRAQCQNLFG HALGEELWHR ASETFAYLPL TANIDGRIFC THGGIPRYRG GVDDRLHILS RSDFPAFESF FQVPEEDTAY MRMCRQMAMD TCWADPAEDE STLDKWGFGA NPRGSGVIVF GSVAVDHFLL NNNFEYIFRA HQEKSDGLKL SKSARVFTIF STSAYVGHEN GAGVVLVADG KIRLIIKSAD DTARTPVVAE EEEDKDEEEE EKEAEIEAEE REEKKNEKMR KSSRLVR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata aacgctccat ttcgttagat cccgatgcgc tcgtgtcaca ccacacgctt cgcacgttcc ataagcaatt actggatggc tgtggggtct tatcccctct ctcctcctac ggcaatgcta tttaccacta cattgtgaag acgtttgcgg ttcgcaatgg ctgtcgtcat agctcctccg acattgaggg ttttggccgc acattatgta gcctctgtgg ggaggtgaag gccgtcctac agagggaacc acgacatgga agtgttgttt ccccatgtta tgtctttggc gacctacatg gtaacttttt ggatctattc tactttctcg ataatcttat ctcgtttcag gatctgcgtt acaccccgca tcgctttctt tttcttggtg actacgtgga tcgcggggag tttagtgtgg aggtgttggc atacctcttc gccatgaaag tgctggcacc agagaaggtg ctgctgctgc gtggcaatca cgaggacaca ctcgtcagtg gggacatctc cggatatggc aacaacagct ttcgagcgca atgtcaaaac ctttttggcc acgctcttgg tgaggaactg tggcaccgtg caagtgagac atttgcctat ctcccactca ctgcaaacat tgatgggcgc atcttctgca cccatggcgg aatcccacgc taccgcggtg gtgtagatga caggctgcat atattaagcc ggagcgattt tcctgcattt gagtctttct ttcaagttcc cgaagaggat acggcataca tgcgtatgtg tcggcagatg gccatggaca catgttgggc cgatcccgct gaggatgaga gcacacttga caagtggggt tttggtgcca atccgcgcgg ttctggcgtt attgtctttg gctccgttgc cgtggatcat tttcttttaa ataataactt tgaatacatc ttccgtgccc accaagagaa gtcagatggg ctgaaactca gtaagagtgc ccgggtcttt acaatattta gcaccagcgc gtatgttggg cacgaaaacg gtgccggtgt tgtacttgtg gcggatggga agattcgact gattattaaa agcgccgatg acaccgctcg tacaccagta gtagcggagg aagaggaaga caaggacgaa gaagaagagg aaaaagaggc tgagattgaa gcggaggaga gggaggagaa aaagaatgaa aaaatgcgaa aatcatcacg tctggtgaga tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat