SSGCID: Target Page

TrcrA.00085.b

UDP-galactose 4-epimerase

CENTER ID:	TrcrA.00085.b
ORGANISM:	Trypanosoma cruzi
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.00085.b.B1.GE35083	full length		1	383

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrA.00085.b.B1.PS01539	full length		1	383

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tc00.1047053506811.190
OrthoMCL:	OG5_126853
RefSeq:	XP_818215.1
UniProt:	Q4DUZ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRVLVCGGAG YIGTHFVRAL LRRTSHSVVI LDSLEATHGR SAHIDTEENL ARNQKCGSEQ LCAGRHAVLE LGDVRDEAFL NDVFTRHAPI DAVVHMCAFI VVPESMRDPL KYYDNNVIGI IRLLQVMRAH HCDKIIFSST AALFGNPAAH ASEGGATVSM EPIHPDAKKH PESPYGETKL VAEWILKDCA YAYGIKSICL RYFNACGADE DGDIGEDHEP ETHLIPLILR VPLATEINKR RKAVQQAPVE EFVSIFGTDY PTPDGTCVRD YIHVYDLSTA HILALDYLAG LDPNEKATFF SAFNLGTSRG YSVREVIEAA RRVTGHPIPV KESARREGDP PVLVASGEEA KKALGWELKY DNIDKIIASA WKFHRSHPLG YSS

NT Sequence

atgagggttt tagtgtgtgg gggcgctggg tacattggga ctcactttgt tcgtgcgctg ctgcgccgaa cgtctcactc cgtcgttatt cttgacagtc tggaggcgac gcacggacgc tccgctcata ttgacaccga agaaaatttg gcgcggaatc aaaagtgcgg ctcggagcag ttgtgcgcgg gacgccacgc cgtgcttgag ttgggagacg tgcgtgatga ggcctttttg aatgacgttt ttactcggca tgcccccatc gatgccgttg tgcacatgtg cgcatttatt gtggtcccag aaagtatgcg tgatcctttg aagtattacg acaataatgt tatcggaatt atccgcctgc tgcaggttat gcgtgcacat cactgcgaca agattatatt ttccagcacg gcggcgttgt ttgggaaccc tgcggcacac gcaagtgaag gcggcgcaac tgtaagcatg gagcctattc atcccgatgc caagaagcat cctgaaagcc catatggaga aacaaaactc gttgccgaat ggattttgaa ggactgcgct tacgcctatg gcatcaaaag tatttgcctg cggtacttca atgcctgtgg ggcggacgag gacggagata tcggtgagga tcacgagcca gagacacacc tcatcccgct tattctgcgc gtgccactgg caacggagat caacaagcgc cgcaaggcag tgcagcaggc ccctgttgag gaatttgttt ccatttttgg tacggactat cccacccctg atgggacgtg tgtgcgtgac tacattcacg tgtatgactt gtccacagct cacattttgg cactggatta cttggcgggg ctagacccga acgagaaggc cacgtttttt tcggcattta atctcgggac ctcacgtgga tactccgtac gagaggtcat tgaggctgct cgccgcgtca cgggccatcc gatacccgtg aaggaatctg cacgtcgcga gggcgatcca ccggtactag tggcatctgg tgaggaggca aagaaggcgc ttggatggga gttgaagtac gacaacattg acaaaatcat tgcctctgca tggaaatttc accggtcaca tccattgggc tattcctctt ag

Details for TrcrA.00085.b.B1.GE35083

HARVESTED ON:	4/10/2012
SEQUENCED ON:	4/11/2012
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	70

Validated AA Sequence

MCAFIVVPES MRDPLKYYDN NVIGIIRLLQ VMRAHHCDKI IFSSTAALFG NPAAHASEGG ATVSMEPIHP DAKKHPESPY GETKLVAEWI LKDCAYAYGI KSICLRYFNA CGADEDGDIG EDHEPETHLI PLILRVPLAT EINKRRKAVQ QAPVEEFVSI FGTDYPTPDG TCVRDYIHVY DLSTAHILAL DYLAGLDPNE KATFFSAFNL GTSRGYSVRE VIEAARRVTG HPIPVKESAR REGDPPVLVA SGEEAKKALG WELKYDNIDK IIASAW

Validated NT Sequence

ttccatgcag aggcaatgat tttgtcaatg ttgtcgtact tcaactccca tccaagcgcc ttctttgcct cctcaccaga tgccactagt accggtggat cgccctcgcg acgtgcagat tccttcacgg gtatcggatg gcccgtgacg cggcgagcag cctcaatgac ctctcgtacg gagtatccac gtgaggtccc gagattaaat gccgaaaaaa acgtggcctt ctcgttcggg tctagccccg ccaagtaatc cagtgccaaa atgtgagctg tggacaagtc atacacgtga atgtagtcac gcacacacgt cccatcaggg gtgggatagt ccgtaccaaa aatggaaaca aattcctcaa caggggcctg ctgcactgcc ttgcggcgct tgttgatctc cgttgccagt ggcacgcgca gaataagcgg gatgaggtgt gtctctggct cgtgatcctc accgatatct ccgtcctcgt ccgccccaca ggcattgaag taccgcaggc aaatactttt gatgccatag gcgtaagcgc agtccttcaa aatccattcg gcaacgagtt ttgtttctcc atatgggctt tcaggatgct tcttggcatc gggatgaata ggctccatgc ttacagttgc gccgccttca cttgcgtgtg ccgcagggtt cccaaacaac gccgccgtgc tggaaaatat aatcttgtcg cagtgatgtg cacgcataac ctgcagcagg cggataattc cgataacatt attgtcgtaa tacttcaaag gatcacgcat actttctggg accacaataa atgcgcacat gtgcacaacg gcatcgatgg gggcatgccg agtaaaaacg tcattcaaaa aggcctcatc acgcacgtct cccaactcaa gcacggcgtg gcgtcccgcg cacaactgct ccgagccgca cttttgattc cgcgccaaat tttcttcggt gtcaatatga gcggagcgtc cgtgcgtcgc ctccagactg tcaagaataa cgacggagtg agacgttcgg cgcagcagcg cacgaacaaa gtgagtccca atgtacccag cgcccccaca cactaaaa

Expected Protein Sequence

MAHHHHHHMR VLVCGGAGYI GTHFVRALLR RTSHSVVILD SLEATHGRSA HIDTEENLAR NQKCGSEQLC AGRHAVLELG DVRDEAFLND VFTRHAPIDA VVHMCAFIVV PESMRDPLKY YDNNVIGIIR LLQVMRAHHC DKIIFSSTAA LFGNPAAHAS EGGATVSMEP IHPDAKKHPE SPYGETKLVA EWILKDCAYA YGIKSICLRY FNACGADEDG DIGEDHEPET HLIPLILRVP LATEINKRRK AVQQAPVEEF VSIFGTDYPT PDGTCVRDYI HVYDLSTAHI LALDYLAGLD PNEKATFFSA FNLGTSRGYS VREVIEAARR VTGHPIPVKE SARREGDPPV LVASGEEAKK ALGWELKYDN IDKIIASAWK FHRSHPLGYS S

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaggg ttttagtgtg tgggggcgct gggtacattg ggactcactt tgttcgtgcg ctgctgcgcc gaacgtctca ctccgtcgtt attcttgaca gtctggaggc gacgcacgga cgctccgctc atattgacac cgaagaaaat ttggcgcgga atcaaaagtg cggctcggag cagttgtgcg cgggacgcca cgccgtgctt gagttgggag acgtgcgtga tgaggccttt ttgaatgacg tttttactcg gcatgccccc atcgatgccg ttgtgcacat gtgcgcattt attgtggtcc cagaaagtat gcgtgatcct ttgaagtatt acgacaataa tgttatcgga attatccgcc tgctgcaggt tatgcgtgca catcactgcg acaagattat attttccagc acggcggcgt tgtttgggaa ccctgcggca cacgcaagtg aaggcggcgc aactgtaagc atggagccta ttcatcccga tgccaagaag catcctgaaa gcccatatgg agaaacaaaa ctcgttgccg aatggatttt gaaggactgc gcttacgcct atggcatcaa aagtatttgc ctgcggtact tcaatgcctg tggggcggac gaggacggag atatcggtga ggatcacgag ccagagacac acctcatccc gcttattctg cgcgtgccac tggcaacgga gatcaacaag cgccgcaagg cagtgcagca ggcccctgtt gaggaatttg tttccatttt tggtacggac tatcccaccc ctgatgggac gtgtgtgcgt gactacattc acgtgtatga cttgtccaca gctcacattt tggcactgga ttacttggcg gggctagacc cgaacgagaa ggccacgttt ttttcggcat ttaatctcgg gacctcacgt ggatactccg tacgagaggt cattgaggct gctcgccgcg tcacgggcca tccgataccc gtgaaggaat ctgcacgtcg cgagggcgat ccaccggtac tagtggcatc tggtgaggag gcaaagaagg cgcttggatg ggagttgaag tacgacaaca ttgacaaaat cattgcctct gcatggaaat ttcaccggtc acatccattg ggctattcct cttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for TrcrA.00085.b.B1.PS01539

PURIFICATION DATe:	6/27/2012
CONCENTRATION:	45.32mg/ml
OBSERVED MW:	43kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	70

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGGG TTTTAGTGTG TGGGGGCGCT GGGTACATTG GGACTCACTT TGTTCGTGCG CTGCTGCGCC GAACGTCTCA CTCCGTCGTT ATTCTTGACA GTCTGGAGGC GACGCACGGA CGCTCCGCTC ATATTGACAC CGAAGAAAAT TTGGCGCGGA ATCAAAAGTG CGGCTCGGAG CAGTTGTGCG CGGGACGCCA CGCCGTGCTT GAGTTGGGAG ACGTGCGTGA TGAGGCCTTT TTGAATGACG TTTTTACTCG GCATGCCCCC ATCGATGCCG TTGTGCACAT GTGCGCATTT ATTGTGGTCC CAGAAAGTAT GCGTGATCCT TTGAAGTATT ACGACAATAA TGTTATCGGA ATTATCCGCC TGCTGCAGGT TATGCGTGCA CATCACTGCG ACAAGATTAT ATTTTCCAGC ACGGCGGCGT TGTTTGGGAA CCCTGCGGCA CACGCAAGTG AAGGCGGCGC AACTGTAAGC ATGGAGCCTA TTCATCCCGA TGCCAAGAAG CATCCTGAAA GCCCATATGG AGAAACAAAA CTCGTTGCCG AATGGATTTT GAAGGACTGC GCTTACGCCT ATGGCATCAA AAGTATTTGC CTGCGGTACT TCAATGCCTG TGGGGCGGAC GAGGACGGAG ATATCGGTGA GGATCACGAG CCAGAGACAC ACCTCATCCC GCTTATTCTG CGCGTGCCAC TGGCAACGGA GATCAACAAG CGCCGCAAGG CAGTGCAGCA GGCCCCTGTT GAGGAATTTG TTTCCATTTT TGGTACGGAC TATCCCACCC CTGATGGGAC GTGTGTGCGT GACTACATTC ACGTGTATGA CTTGTCCACA GCTCACATTT TGGCACTGGA TTACTTGGCG GGGCTAGACC CGAACGAGAA GGCCACGTTT TTTTCGGCAT TTAATCTCGG GACCTCACGT GGATACTCCG TACGAGAGGT CATTGAGGCT GCTCGCCGCG TCACGGGCCA TCCGATACCC GTGAAGGAAT CTGCACGTCG CGAGGGCGAT CCACCGGTAC TAGTGGCATC TGGTGAGGAG GCAAAGAAGG CGCTTGGATG GGAGTTGAAG TACGACAACA TTGACAAAAT CATTGCCTCT GCATGGAAAT TTCACCGGTC ACATCCATTG GGCTATTCCT CTTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT