TrcrA.00019.a
prostaglandin f synthase
| CENTER ID: | TrcrA.00019.a |
| ORGANISM: | Trypanosoma cruzi |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| TrcrA.00019.a.B1.GE35081 | full length | 1 | 282 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| TrcrA.00019.a.B1.PW35698 | full length | 1 | 282 |
Structures
4FZI
DEPOSITED: 7/6/2012
DETERMINATION: XRay
CLONE: TrcrA.00019.a.B1.GE35081
PROTEIN: TrcrA.00019.a.B1.PW35698
4GIE
DEPOSITED: 8/8/2012
DETERMINATION: XRay
CLONE: TrcrA.00019.a.B1.GE35081
PROTEIN: TrcrA.00019.a.B1.PW35698
Publications by SSGCID
Structures of prostaglandin F synthase from the protozoa Leishmania major and Trypanosoma cruzi with NADP.
Barnes SR, Edwards TE, Fairman JW, Lorimer DD, Moen SO, Myler PJ, Nakazawa-Hewitt S, Staker BL, Sullivan A, Van Voorhis WC
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2015
volume 71, issue Pt 5, pages 609-14
PMID: 25945716; PMCID: PMC4361283
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | TritrypDB:Tc00.1047053511287.49 |
| RefSeq: | XP_814362.1 |
| UniProt: | Q4DJ07 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNCNYNCVTL HNSARMPQLG LGVWRAQDGA ETANAVRWAI EAGYRHIDTA CIYNNEKGVG QGIRESGVPR EEVWVTTKVW NSDQGYEKTL AAFERSRELL GLEYIDLYLI HWPGKKKFVD TWKALEKLYE EKKVRAIGVS NFEPHHLTEL FKSCKIRPMV NQVELHPLFQ QRTLREFCKQ HNIAITAWSP LGSGDRTGFL KNHVLGEIAK KHNKSPAQVV IRWDIQHGIV TIPKSTNKGR IQENFNVWDF KLTEEEMRQI DELNEDKRIG GHPDNFFPGG EE
NT Sequence
atgaattgca attacaactg tgtgacactc cataactccg cgcgaatgcc gcagcttggc ttgggtgttt ggcgggcaca ggacggagcc gagacggcga atgcggtccg gtgggcaatt gaggcaggct accgccacat cgacacggca tgcatctaca acaacgaaaa gggcgtgggg caggggatcc gcgaatcggg agtgccccgt gaggaggtgt gggtaacaac aaaagtatgg aattcagatc aaggatacga aaagacgctt gcggcctttg aacgcagccg tgagctgctc ggattggagt acattgatct ctacttaatt cactggccgg ggaagaagaa gtttgttgac acatggaaag cattggagaa gctctacgag gagaagaagg tgcgggccat tggcgtttcc aactttgagc cacaccacct cacggagctt tttaaaagct gcaaaattcg gccgatggtc aaccaagtgg agctgcaccc gctgttccaa cagcgtactt tgcgggagtt ctgcaagcaa cacaacattg ccatcacagc ctggtctccg cttggcagcg gggaccggac gggttttctg aagaatcacg tgctggggga gattgccaag aagcacaata aatccccggc gcaggttgtc atccgctggg acattcagca cggtatcgtg acaattccca agtccacaaa taaggggcgc attcaagaaa actttaatgt ttgggacttt aaacttacgg aagaggagat gcgtcaaatc gatgaactca acgaggataa acgcatcggc ggacaccctg ataatttttt ccctggtgga gaggagtga
Details for TrcrA.00019.a.B1.GE35081
| HARVESTED ON: | 4/10/2012 |
| SEQUENCED ON: | 4/11/2012 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 33kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 96 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMN CNYNCVTLHN SVRMPQLGLG VWRAQDGAET ANAVRWAIEA GYRHIDTAYI YSNERGVGQG IRESGVPREE VWVTTKVWNS DQGYEKTLAA FERSRELLGL EYIDLYLIHW PGKKKFVDTW KALEKLYEEK KVRAIGVSNF EPHHLTELFK SCKIRPMVNQ VELHPLFQQR TLREFCKQHN IAITAWSPLG SGEEAGILKN HVLGEIAKKH NKSPAQVVIR WDIQHGIVTI PKSTNKGRIQ ENFNVWDFKL TEEEMRQIDE LNEDKRIGAD PDNFFPGGEE
Validated NT Sequence
cctatcttac tcacttactc ctctccacca gggaaaaaat tatcggggtc tgcaccgatg cgcttatcct cgttgagttc atcgatttga cgcatttcct cttccgtaag cttaaagtcc caaacattaa agttttcttg aatgcgcccc ttatttgtgg acttgggaat tgtcacgata ccgtgttgaa tgtcccagcg gatgacaacc tgcgcagggg atttattgtg cttcttggca atctccccca gcacgtgatt cttcagaata ccagcctctt ccccgctgcc aagcggagac caggctgtga tggcaatgtt gtgttgcttg cagaactccc gcaaagtacg ctgttggaac agcgggtgca gctccacttg gttcaccatc ggccgaattt tgcagctttt aaaaagctcc gtgaggtggt gtggctcaaa gttggaaacg ccaatggccc gcaccttctt ctcctcgtag agcttctcca atgccttcca tgtgtcaaca aacttcttct tccccggcca gtgaattaag tagagatcaa tgtactccag tccgagcagc tcacggctgc gttcaaaggc cgcaagcgtc ttttcgtatc cttgatctga attccatact tttgttgtta cccacacctc ctcacggggc actcccgatt cgcggatccc ctgccccacg cccctttcgt tgctgtagat gtatgccgta tcaatgtggc ggtagcctgc ctcaattgcc caccggaccg cattcgccgt ctcggctccg tcctgtgccc gccaaacacc caagccaagc tgcggcattc gcacggagtt atggagtgtc acacagttgt aattgcaatt catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMN CNYNCVTLHN SARMPQLGLG VWRAQDGAET ANAVRWAIEA GYRHIDTACI YNNEKGVGQG IRESGVPREE VWVTTKVWNS DQGYEKTLAA FERSRELLGL EYIDLYLIHW PGKKKFVDTW KALEKLYEEK KVRAIGVSNF EPHHLTELFK SCKIRPMVNQ VELHPLFQQR TLREFCKQHN IAITAWSPLG SGDRTGFLKN HVLGEIAKKH NKSPAQVVIR WDIQHGIVTI PKSTNKGRIQ ENFNVWDFKL TEEEMRQIDE LNEDKRIGGH PDNFFPGGEE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatt gcaattacaa ctgtgtgaca ctccataact ccgcgcgaat gccgcagctt ggcttgggtg tttggcgggc acaggacgga gccgagacgg cgaatgcggt ccggtgggca attgaggcag gctaccgcca catcgacacg gcatgcatct acaacaacga aaagggcgtg gggcagggga tccgcgaatc gggagtgccc cgtgaggagg tgtgggtaac aacaaaagta tggaattcag atcaaggata cgaaaagacg cttgcggcct ttgaacgcag ccgtgagctg ctcggattgg agtacattga tctctactta attcactggc cggggaagaa gaagtttgtt gacacatgga aagcattgga gaagctctac gaggagaaga aggtgcgggc cattggcgtt tccaactttg agccacacca cctcacggag ctttttaaaa gctgcaaaat tcggccgatg gtcaaccaag tggagctgca cccgctgttc caacagcgta ctttgcggga gttctgcaag caacacaaca ttgccatcac agcctggtct ccgcttggca gcggggaccg gacgggtttt ctgaagaatc acgtgctggg ggagattgcc aagaagcaca ataaatcccc ggcgcaggtt gtcatccgct gggacattca gcacggtatc gtgacaattc ccaagtccac aaataagggg cgcattcaag aaaactttaa tgtttgggac tttaaactta cggaagagga gatgcgtcaa atcgatgaac tcaacgagga taaacgcatc ggcggacacc ctgataattt tttccctggt ggagaggagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for TrcrA.00019.a.B1.PW35698
| PURIFICATION DATe: | 5/14/2012 |
| CONCENTRATION: | 37.05mg/ml |
| OBSERVED MW: | 33kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 14 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 96 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN CNYNCVTLHN SVRMPQLGLG VWRAQDGAET ANAVRWAIEA GYRHIDTAYI YSNERGVGQG IRESGVPREE VWVTTKVWNS DQGYEKTLAA FERSRELLGL EYIDLYLIHW PGKKKFVDTW KALEKLYEEK KVRAIGVSNF EPHHLTELFK SCKIRPMVNQ VELHPLFQQR TLREFCKQHN IAITAWSPLG SGEEAGILKN HVLGEIAKKH NKSPAQVVIR WDIQHGIVTI PKSTNKGRIQ ENFNVWDFKL TEEEMRQIDE LNEDKRIGAD PDNFFPGGEE
Validated NT Sequence
cctatcttac tcacttactc ctctccacca gggaaaaaat tatcggggtc tgcaccgatg cgcttatcct cgttgagttc atcgatttga cgcatttcct cttccgtaag cttaaagtcc caaacattaa agttttcttg aatgcgcccc ttatttgtgg acttgggaat tgtcacgata ccgtgttgaa tgtcccagcg gatgacaacc tgcgcagggg atttattgtg cttcttggca atctccccca gcacgtgatt cttcagaata ccagcctctt ccccgctgcc aagcggagac caggctgtga tggcaatgtt gtgttgcttg cagaactccc gcaaagtacg ctgttggaac agcgggtgca gctccacttg gttcaccatc ggccgaattt tgcagctttt aaaaagctcc gtgaggtggt gtggctcaaa gttggaaacg ccaatggccc gcaccttctt ctcctcgtag agcttctcca atgccttcca tgtgtcaaca aacttcttct tccccggcca gtgaattaag tagagatcaa tgtactccag tccgagcagc tcacggctgc gttcaaaggc cgcaagcgtc ttttcgtatc cttgatctga attccatact tttgttgtta cccacacctc ctcacggggc actcccgatt cgcggatccc ctgccccacg cccctttcgt tgctgtagat gtatgccgta tcaatgtggc ggtagcctgc ctcaattgcc caccggaccg cattcgccgt ctcggctccg tcctgtgccc gccaaacacc caagccaagc tgcggcattc gcacggagtt atggagtgtc acacagttgt aattgcaatt catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHMN CNYNCVTLHN SARMPQLGLG VWRAQDGAET ANAVRWAIEA GYRHIDTACI YNNEKGVGQG IRESGVPREE VWVTTKVWNS DQGYEKTLAA FERSRELLGL EYIDLYLIHW PGKKKFVDTW KALEKLYEEK KVRAIGVSNF EPHHLTELFK SCKIRPMVNQ VELHPLFQQR TLREFCKQHN IAITAWSPLG SGDRTGFLKN HVLGEIAKKH NKSPAQVVIR WDIQHGIVTI PKSTNKGRIQ ENFNVWDFKL TEEEMRQIDE LNEDKRIGGH PDNFFPGGEE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATT GCAATTACAA CTGTGTGACA CTCCATAACT CCGCGCGAAT GCCGCAGCTT GGCTTGGGTG TTTGGCGGGC ACAGGACGGA GCCGAGACGG CGAATGCGGT CCGGTGGGCA ATTGAGGCAG GCTACCGCCA CATCGACACG GCATGCATCT ACAACAACGA AAAGGGCGTG GGGCAGGGGA TCCGCGAATC GGGAGTGCCC CGTGAGGAGG TGTGGGTAAC AACAAAAGTA TGGAATTCAG ATCAAGGATA CGAAAAGACG CTTGCGGCCT TTGAACGCAG CCGTGAGCTG CTCGGATTGG AGTACATTGA TCTCTACTTA ATTCACTGGC CGGGGAAGAA GAAGTTTGTT GACACATGGA AAGCATTGGA GAAGCTCTAC GAGGAGAAGA AGGTGCGGGC CATTGGCGTT TCCAACTTTG AGCCACACCA CCTCACGGAG CTTTTTAAAA GCTGCAAAAT TCGGCCGATG GTCAACCAAG TGGAGCTGCA CCCGCTGTTC CAACAGCGTA CTTTGCGGGA GTTCTGCAAG CAACACAACA TTGCCATCAC AGCCTGGTCT CCGCTTGGCA GCGGGGACCG GACGGGTTTT CTGAAGAATC ACGTGCTGGG GGAGATTGCC AAGAAGCACA ATAAATCCCC GGCGCAGGTT GTCATCCGCT GGGACATTCA GCACGGTATC GTGACAATTC CCAAGTCCAC AAATAAGGGG CGCATTCAAG AAAACTTTAA TGTTTGGGAC TTTAAACTTA CGGAAGAGGA GATGCGTCAA ATCGATGAAC TCAACGAGGA TAAACGCATC GGCGGACACC CTGATAATTT TTTCCCTGGT GGAGAGGAGT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT