TrbrB.18664.d

kinetoplastid kinetochore protein 2 (kkt2)

CENTER ID: TrbrB.18664.d
ORGANISM: Trypanosoma brucei GUTat10.1
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrB.18664.d.B3.GE37004 RING/U-box domain 579 672
TrbrB.18664.d.B4.GE43104 PHoranyi kinase domain truncation 67 361
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tb927.11.10520
RefSeq: XP_829116.1
UniProt: Q383M7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFNVSPASRD RVRSESQRTP RPRSSLSMPR ELSYTPAISS IPSSLHTPFI QKCYVQGDNS TEGQQQQQQQ PPDHMTVVFE SDNMQLTTTS LLGKGGFGRV YVAQSSGGEL CALKVSSKQM TDGDWERLRK EVALMSHFSQ HPNVVKLIAA GRDRNFAYVA MECCASRSLH DIINKHGLEV PEILWVGYAL IDTIAFLHAK GCIHRDLKPQ NLLFDFDGNL KISDFGLSSN VTESEPRKTV AGTAMYMAPE IAGAVYKRMT NDNSSSSLRY GQEVDTWSIG VVLYVMLTRM NPYAQALENE GAHEMNKTQK TLTLFSAVAG AAWHWPAGWR GDKELSDVVN QILHRNPAQR ATLQDILQHP VWDRRPLSCP LTLLQKLNLV ERRPFTRTGA VGRSCSRAPE GMQPQYATKT AEVVLLEGLN QVIQVEESTR TQLVLESNEV INLIFGTLKL HASEANGRRF IVIDEKGCRK NIEDMLLTTR PVRCRSHELV STAVCAKQPK RGTRVGSSLR PQRESSVLLA GPLAFGQTQD AGEIVRAASD RYAVVFSGRE TSTRWSLRNV ISLPRELNAA IEEVTCINKH QMKKLIKIPL GYVGFDCNVC DSAIDDISIE KPVFRCHKCD YDLCMNCAYE GKIKDVNFVC VSCMKKFASS SKLEAHSAKC RGPSMSPSAR CSSRRNTMLW EQLENEGGSL LDIRLPTETK PRRSARTSCR TSGGRESSGG RISIGDSYVP GPEDLGTMVV AHRDANFPEM PKFSTQSDSK ASSPFGEKEG ADKNYRQSFS FELPPQVRLS KHRRDKGAEP RSSEELREIV DELTPTPKRV RTEARQNSCE PYAPQVNDAG LVVGIAARSR VEREGSLRGE QQSRNVVVIR ADNAAKPQDL RKQSQVPRSA SSSQPGSRME SQAIDTLSKP APKNVAVREY RSASGQRDPL VPRTSLPSAQ TGEIFGAPPL PRIVTSGRSS VPRSMSYTLP SAAGVARRQT SQPLVNLLGG VPVGAYGGGS FNPGERNASI SNTNRTVGPG QGGTAYLALP RDKQNRDRFV DDFLSGAWVR VYSFIGSEVV VMYYSVQPGR YGALFPTEEG AATAVLDIHS KLVLYVPRMD KDTVTRTQCH PNVLSFFQDE IRILPATSAE KTLGGVLRGI MGFVSELTKC RGEGEKYAAA QSAYIHQREK GAVPAGTKFA YVRKAFPDPA GSFVLFRLSN LRSQVVFNNA LLDIRWQSDK NHNVGQKYYV RPNGEAGPFT AEHSGILNHV NLVMRNVYRK
NT Sequence
atgttcaatg tctcaccagc gagtcgtgac cgcgtgcgtt ctgagagtca gcgcaccccg cgcccccgca gctcactctc catgccacgt gaattgtcct acacacccgc catatcttcc attccttcga gcctgcacac accgtttatt caaaagtgct acgttcaggg ggacaattcc actgagggtc agcagcagca gcagcagcaa ccgcctgatc acatgactgt cgtatttgaa agtgataaca tgcaactgac aacaacaagt ttgctcggga aaggtgggtt tggtcgagtg tacgtggccc agtcaagtgg cggggagttg tgcgccttga aagtatcgtc gaaacaaatg acggatggtg actgggagcg gttgcgcaaa gaggtggcgc ttatgagtca cttctcgcag caccccaacg tggtgaagct aattgctgcc ggtcgagata ggaattttgc gtatgtggcg atggagtgct gtgcgagtcg gtcgctgcat gacattatta acaaacatgg tctcgaagtg ccagaaattt tgtgggtcgg ctatgcactc attgatacaa tcgcttttct gcacgccaag ggttgcatac accgggacct aaagccacag aaccttttgt ttgacttcga tggaaacctg aagataagtg attttggact ttcaagcaac gttaccgaat ctgaaccacg gaagacggtt gccgggacgg caatgtatat ggcacccgaa attgctggcg cagtgtataa acgcatgacc aacgataaca gctctagttc actgcgctac ggtcaggagg ttgacacatg gagcattggt gtggtgttgt acgtcatgtt gacgcgcatg aacccgtacg cgcaggcgct tgaaaatgaa ggtgcacacg agatgaacaa gacgcagaag acactcacct tatttagtgc ggttgcgggg gccgcttggc actggccggc gggttggaga ggcgacaagg aactaagtga tgttgtcaat caaatactgc atcgcaatcc agctcagcga gccacgctac aagatatact gcagcaccct gtttgggacc gacggccgct ttcgtgtccg ctcacgctgc tgcaaaaact gaatttagtc gagcgacgac cgtttacccg aaccggagcg gtaggccgct catgcagccg tgctcctgaa ggtatgcagc cccaatatgc caccaaaacg gcagaagttg tgctcctaga ggggctaaac caagtgattc aagtcgaaga gagcactcgt actcaactcg tccttgaaag caatgaggtg ataaatctca tattcggaac cctgaagctg catgcctcag aggctaacgg aaggcggttt attgtgatag atgagaaagg ttgccgcaag aacatcgaag acatgttgct gaccactcgc ccggtgcgtt gtcgcagcca tgagttagtg tcgacagcgg tgtgtgcaaa acagcccaaa cgtggaacaa gggtcggttc ctcattaagg ccgcaaaggg aatccagtgt tttgttggcg ggtccattag ctttcggcca gactcaagat gctggagaga ttgtccgcgc agcgtctgac aggtacgctg tggtgttcag tggacgtgaa acctccacac ggtggtctct ccgcaatgtc atctcactcc ctcgagaact taacgctgca attgaggagg ttacgtgcat taacaaacac caaatgaaga aacttatcaa aatacccctg ggttatgtcg ggtttgattg caacgtatgc gacagtgcga ttgatgatat tagtatcgag aaaccagttt ttcgatgtca taagtgcgac tacgacctct gcatgaattg tgcttacgag gggaaaataa aggacgttaa ctttgtttgt gtttcatgca tgaaaaagtt tgcgtcctct tcaaagctcg aagcacattc agctaagtgc cgtggcccaa gcatgagccc ctcggcacgc tgctcttccc gtcgaaacac gatgctttgg gagcaattgg aaaacgaggg cggaagtctg ctggatatcc ggttgcctac cgaaactaaa ccgcgacgta gtgcccgtac atcttgccgt acctccggtg gtcgagagtc gtcgggtggt cgcattagta ttggtgacag ctatgtacca ggaccggagg acttaggcac aatggtagta gcgcaccgtg acgccaattt ccctgaaatg ccaaaattct ccactcagag cgatagcaag gcaagctccc ctttcggtga gaaggagggc gctgataaaa attataggca gagtttctcc tttgaacttc cccctcaggt tcggctctct aaacatcgta gggataaggg agcggaacca cggagttcgg aagaattgcg cgagattgta gatgagctta cgccaacacc taaaagggtg cgtacagaag cacggcagaa ttcttgtgag ccatatgctc cccaagtgaa tgacgcaggc ttggttgtcg gcattgctgc gcggtctcgt gttgaaaggg agggaagtct gcgtggggaa cagcaatccc gtaacgtcgt agtaatccgt gcagacaacg cggcgaaacc acaagacctg cgaaagcagt ctcaggttcc tcgaagcgct tcgtcaagtc aacctggtag taggatggag tcgcaagcta tcgatactct ctcgaagccg gcccctaaga atgtggcagt acgagagtat cggtccgcgt ccggccagcg cgatccactg gtgccgagaa cgtcactgcc ttcagctcag actggagaaa ttttcggtgc accgcctctc ccacgaatcg tcacctccgg gaggtctagc gtaccacgtt caatgtcgta cacattgcca tctgctgctg gtgttgcgcg acgccagaca tcccagcctt tagtgaactt acttggaggg gtccctgtcg gggcgtatgg gggcggatct ttcaaccccg gcgagcgcaa tgcttccatc agcaacacca acagaactgt tggaccgggg caaggtggga cggcgtacct ggcccttcct cgggacaagc aaaaccggga tcgctttgtt gatgacttcc taagcggggc gtgggtaagg gtttactctt tcattggttc tgaagttgtt gtgatgtatt attctgtgca acccggtagg tacggtgccc tcttccccac agaggaggga gcagctacgg cagttctgga catacactct aagttggtgc tatacgtgcc gcgtatggat aaggataccg ttactcggac ccagtgccat ccgaatgttc taagtttctt tcaagacgaa atacgcattc tacccgctac aagtgcagag aaaaccctcg gtggtgtgct tcgtggcata atgggtttcg ttagtgaact tacgaaatgt cgtggggagg gagaaaagta tgcagcagcg cagtctgcat acattcacca acgtgagaag ggtgcggtcc ccgcgggcac aaagttcgcg tatgtgagaa aggcgttccc agatccagca ggctctttcg tgttgttccg tttgtccaac ctgcggtcgc aggtagtttt taacaacgca ctcctagaca tccgttggca aagcgacaag aaccataatg ttgggcaaaa gtactacgtg cggcccaacg gcgaggccgg accatttact gcggagcact caggtatcct caatcacgta aatttggtga tgcgcaacgt ctaccggaaa tga
Details for TrbrB.18664.d.B3.GE37004
HARVESTED ON: 5/21/2013
SEQUENCED ON: 5/23/2013
EXPECTED MW: 11kDa
OBSERVED MW: 11kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK HQMKKLIKIP LGYVGFDCNV CDSAIDDISI EKPVFRCHKC DYDLCMNCAY QGKIKDVNFV CVSCMKKFAS SSKLEAHSAK CRGPSMSPSA RCS
Validated NT Sequence
tactcactta agagcagcgt gccgaggggc tcatgcttgg gccacggcac ttagctgaat gtgcttcgag ctttgaagag gacgcaaact ttttcatgca tgaaacacaa acaaagttaa cgtcctttat ttttccctgg taagcacaat tcatgcagag gtcgtagtcg cacttatgac atcgaaaaac tggtttctcg atactaatat catcaatcgc actatcgcat acgttgcaat caaacccgac ataacccaag ggtattttga taagtttctt catttggtgt ttcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHKH QMKKLIKIPL GYVGFDCNVC DSAIDDISIE KPVFRCHKCD YDLCMNCAYE GKIKDVNFVC VSCMKKFASS SKLEAHSAKC RGPSMSPSAR CS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaacacc aaatgaagaa acttatcaaa atacccctgg gttatgtcgg gtttgattgc aacgtatgcg acagtgcgat tgatgatatt agtatcgaga aaccagtttt tcgatgtcat aagtgcgact acgacctctg catgaattgt gcttacgagg ggaaaataaa ggacgttaac tttgtttgtg tttcatgcat gaaaaagttt gcgtcctctt caaagctcga agcacattca gctaagtgcc gtggcccaag catgagcccc tcggcacgct gctcttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for TrbrB.18664.d.B4.GE43104
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/23/2019
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMQ QQQPPDHMTV VFESDNMQLT TTSLLGKGGF GRVYVAQSSG GELCALKVSS KQMTDGDWER LRKEVALMSH FSQHPNVVKL IAAGRDRNFA YVAMECCASR SLHDIINKHG LEVPEILWVG YALIDTIAFL HAKGCIHRDL KPQNLLFDFD GNLKISDFGL SSNVTESEPR KTVAGTAMYM APEIAGAVYK RMTNDNSSSS LRYGQEVDTW SIGVVLYVML TRMNPYAQAL ENEGAHEMNK TQKTLTLFSA VAGAAWHWPA GWRGDKELSD VVNQILHRNP DQRAXXXXXX QHPV
Validated NT Sequence
ttactcactt aaacagggtg ctgcannnnn nnnnnnnnnn tggctcgctg atctggattc cgatgcagta tttgattgac aacatcactt agttccttgt cgcctctcca acccgctggc cagtgccaag cggcccccgc aaccgcacta aataaggtga gtgtcttctg cgtcttgttc atctcgtgtg caccttcatt ttcaagcgcc tgcgcgtacg ggttcatgcg cgtcaacatg acgtacaaca ccacaccaat gctccatgtg tcgacctcct gaccgtagcg cagcgaacta gagctgttat cgttggtcat gcgtttatac actgcgccag caatttcggg tgccatatac attgccgtcc cggcaaccgt cttccgtggt tcagattcgg taacgttgct tgaaagtcca aaatcactta tcttcaggtt tccatcgaag tcaaacaaaa ggttctgtgg ctttaggtcc cggtgtatgc aacccttggc gtgcagaaag gcgattgtat caatgagtgc atagccgacc cacaaaattt ctggcacttc gagaccatgt ttgttaataa tgtcatgcag cgaccgactc gcacagcact ccatcgccac atacgcgaaa ttcctatctc gaccggcagc aattagcttc accacgttgg ggtgctgcga gaagtgactc ataagcgcca cctctttgcg caaccgctcc cagtcaccat ccgtcatttg tttcgacgat actttcaagg cgcacagctc cccgccactt gactgggcca cgtacactcg accaaaccca cctttcccga gcaaacttgt tgttgtcagt tgcatgttat cactttcaaa tacgacagtc atgtgatcag gcggttgctg ctgctgcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHQQ QQPPDHMTVV FESDNMQLTT TSLLGKGGFG RVYVAQSSGG ELCALKVSSK QMTDGDWERL RKEVALMSHF SQHPNVVKLI AAGRDRNFAY VAMECCASRS LHDIINKHGL EVPEILWVGY ALIDTIAFLH AKGCIHRDLK PQNLLFDFDG NLKISDFGLS SNVTESEPRK TVAGTAMYMA PEIAGAVYKR MTNDNSSSSL RYGQEVDTWS IGVVLYVMLT RMNPYAQALE NEGAHEMNKT QKTLTLFSAV AGAAWHWPAG WRGDKELSDV VNQILHRNPA QRATLQDILQ HPV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcagcagc agcaaccgcc tgatcacatg actgtcgtat ttgaaagtga taacatgcaa ctgacaacaa caagtttgct cgggaaaggt gggtttggtc gagtgtacgt ggcccagtca agtggcgggg agttgtgcgc cttgaaagta tcgtcgaaac aaatgacgga tggtgactgg gagcggttgc gcaaagaggt ggcgcttatg agtcacttct cgcagcaccc caacgtggtg aagctaattg ctgccggtcg agataggaat tttgcgtatg tggcgatgga gtgctgtgcg agtcggtcgc tgcatgacat tattaacaaa catggtctcg aagtgccaga aattttgtgg gtcggctatg cactcattga tacaatcgct tttctgcacg ccaagggttg catacaccgg gacctaaagc cacagaacct tttgtttgac ttcgatggaa acctgaagat aagtgatttt ggactttcaa gcaacgttac cgaatctgaa ccacggaaga cggttgccgg gacggcaatg tatatggcac ccgaaattgc tggcgcagtg tataaacgca tgaccaacga taacagctct agttcactgc gctacggtca ggaggttgac acatggagca ttggtgtggt gttgtacgtc atgttgacgc gcatgaaccc gtacgcgcag gcgcttgaaa atgaaggtgc acacgagatg aacaagacgc agaagacact caccttattt agtgcggttg cgggggccgc ttggcactgg ccggcgggtt ggagaggcga caaggaacta agtgatgttg tcaatcaaat actgcatcgc aatccagctc agcgagccac gctacaagat atactgcagc accctgtttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat