SSGCID: Target Page

TrbrB.18664.c

Protein kinase, putative (kkt3)

CENTER ID:	TrbrB.18664.c
ORGANISM:	Trypanosoma brucei GUTat10.1
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	AA START	AA STOP
TrbrB.18664.c.B2.GE37002	STPK domain	35	329
TrbrB.18664.c.B3.GE37003	C-terminal Zn-finger-like domain	628	960
TrbrB.18664.c.B4.GE43103	PHoranyi kinase domain truncation	1	328

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tb927.9.10920
RefSeq:	XP_827369.1
UniProt:	Q38DT1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIGTVEQILY EEGCVRAVRV RLPSRDSSQG KCLSLEACER IGQGSFGTVY RASCDEYPRL ALKITTGKVT RLKQELEVLG RVCTKGKLLL PRFLFGALNK SGDLMAVGME LCFPHTLHDF LLSKCLTDEA DKLFVAYQVV QAVAYVHEQH CIHRDVKLQN FVFDLDGNLK LIDFGLATSV WNPPPGDVVA GTIAFMAPEM AHNALHRDQR VSVGAAADVW SVGMVLFSIF AQRNPYASTG APRMTERTAD TNMHNTAAIP TDASPNDKQS HEIPKENAEL LRRVAAAEWS WPSGCSVSRK LRGLVDFVLV PDPQNRPDIN ALLMRPEWGD RRRATPRVVT TFLGVEDDFL LSHDESHLLR AVEQRSADVN ASLTESRIRG SDEEETEEGH RSSLKFVARE ELSVGGVPIH QVYDVRPDPK VKKPIREISS VIAEETERSL KRSRSRSARA AASSASRANS RVNARSRTVT PVPCTTEGRG NADFPTGSAA ASLVLLEAEG KEETKTGLNI GQRSPQGRQP RSNNVDRAKG INLVGKRDPK PKSANGTVQR STSPIEHTVD TKRGGGNNSH GVGARRGSTV TARKKKGNGA EVVSGFVTME NAVRNVKTSL LLLEHSCMVT RLSTVVTELF DREHMVWLEK EQRKSATHPH TFREMGRANK KYRYGFVCDV CCFEFEPVGS SMYFFHCQCG RDMCPKCYEE YANNYTCDAC GREFASSGAL RRHSCSCVKR LNDAATVNKP RGRSQGRRSR SVPTEAARTG VKSRLEVKPV SPKRGRPKRR ASVPALERKR SREPAAEVVG IVARRKAERL SMSKSEVVPP PRRFEGIPRT LSGEWKPMER LSVGSRSVPP TPEERHMLLN GDWIRYYHFY PMEEEGGDSV AVTYHIQPGR TGVTFFNHSF SVHSAVLSVL EHIVYVVDRV DIEEDNDVAR ILSLAQALNE EKKIYDVLQL VETHDTHMLK QRRSPGIMSV YCPPQTAFQC NGDPFVFVRW YRFHMENSMS GFMLSNGAVQ VFVGGKYELR WLDDNRKFIV RSNGVCEVLD EEKFPLSEEL NQMLYGGV

NT Sequence

atgattggca cagtagaaca aatattgtac gaggagggct gcgtgcgtgc tgtgagggtg cgattgcctt ctcgtgattc atctcaagga aagtgtttat ccctggaggc gtgcgaacgc attggacaag gttcttttgg caccgtttac agagcctctt gtgatgaata tccgagattg gcattaaaga ttacaacagg aaaggttaca cggctcaagc aggaactgga agtgcttgga agggtctgca caaaaggaaa actcctgctg ccacggtttt tatttggtgc attgaataag tccggggacc tcatggctgt ggggatggaa ttgtgtttcc cacacacact gcatgacttc cttctgtcaa agtgtctaac agatgaggcg gacaagttgt tcgttgcata ccaagtagtg caagcagtgg cgtacgtcca tgaacaacat tgcatccaca gggatgttaa acttcagaat tttgtcttcg atttggatgg taacttaaaa ctcatagatt ttggacttgc gacgtctgtg tggaaccctc ctcctggaga tgtagtcgct ggcactatcg cattcatggc cccggagatg gctcataacg ctttacacag ggatcagaga gttagcgttg gtgcggcagc agatgtgtgg tctgtgggaa tggtgctttt ttcaattttc gcacaacgga atccgtatgc ctccacaggt gccccgcgga tgacagagag aactgctgac accaatatgc ataacactgc tgccataccc accgacgcct ctccgaatga caagcagtcc cacgaaatac cgaaggaaaa cgctgagttg cttcgtcgtg tcgctgccgc tgaatggtcg tggcctagtg gttgtagcgt ctccaggaag ctacggggac ttgtggactt cgtccttgtc cctgatccac aaaatcgtcc agatatcaac gctctcctca tgagaccgga atggggcgat aggcgcagag caaccccaag ggtggtaact actttcctcg gcgtggagga tgattttcta ctttcgcacg atgagtctca tttgctgcgt gctgtcgagc agcgaagtgc cgatgtgaac gcaagcctca cagaaagcag aataagaggt tctgacgagg aagagacgga ggagggacac cgttcgtctt tgaaattcgt cgctagggag gagttatcgg tgggtggtgt gccgatacat caagtctacg atgtaaggcc cgaccctaaa gtgaaaaagc ctattcgtga gatttcgtct gttatagccg aggaaacaga gaggagcctg aaacggagca ggtcgagatc cgctagggcc gcggcttcgt cagcgtcacg tgcaaactct cgcgtgaatg caagaagtag gacagtaacg cctgttcctt gcacaacgga aggccgtgga aatgcggatt tccctacagg cagtgccgca gcctcattgg tattgttgga ggcggagggc aaagaagaga cgaaaacagg attgaatatc ggccagcgct caccacaggg gcgtcaacct cgttcgaaca atgtggatcg agcgaagggt ataaatctcg tggggaaaag agatcccaag ccaaaaagtg caaatggcac tgtgcaacgg agcacatcgc ctattgaaca tacggttgat accaaacgag ggggagggaa caatagccat ggagttgggg caaggcgagg cagcactgtt acggcccgta aaaagaaggg gaatggggcg gaggttgtat caggatttgt taccatggag aatgctgtgc gaaacgtcaa aacgtcgctt ttactcttag agcattcatg catggtgacc cggctatcca ctgtcgtgac ggagttattt gatcgagagc atatggtgtg gttggagaaa gagcagcgaa agtccgccac tcacccgcat acattcaggg aaatgggtcg cgcaaacaag aagtacaggt acggcttcgt atgtgatgtc tgctgttttg aattcgaacc agttggatca agtatgtact tctttcactg ccaatgcggt agggacatgt gtccaaaatg ttacgaggag tatgccaata attacacctg cgacgcctgc ggcagggagt ttgccagttc aggtgcgctt cgcaggcaca gttgctcgtg cgtgaagagg ctaaatgatg cagctacagt gaacaagcca agggggagat ctcaggggcg tcgcagtcgt agtgttccaa ccgaagctgc taggactggt gtaaaaagtc ggttggaggt gaaaccagtc agtccgaagc gtggtcggcc gaaacgacga gcatcggtgc cagcactgga acgcaagagg tctcgagagc cggcggctga ggtagtggga attgtggcgc ggaggaaggc agagcgtttg tcaatgagta aatcggaagt tgtaccgccg ccaaggaggt tcgaaggaat accccgcacg ctaagtggtg aatggaaacc gatggagcgt ctctctgtcg ggtctcggag tgtcccaccc acacctgagg aacgtcacat gctgcttaac ggtgattgga tacgctacta ccacttttat cctatggagg aagaaggagg cgactcagtc gctgtcacat atcatattca gccgggacgt actggtgtta catttttcaa ccatagtttt tctgtgcact cagctgtgct gtcagtgttg gaacacatcg tatacgtcgt agatcgtgtt gatatcgagg aagataatga cgtggcgcgt atcttgtcgt tggcacaagc attgaatgag gagaagaaga tctacgatgt ccttcaattg gtcgaaaccc acgacacaca tatgttaaaa cagcggcggt ctcccggtat tatgtctgtt tattgcccac cacaaacagc atttcaatgc aatggtgatc cctttgtatt tgttcgctgg taccgcttcc atatggaaaa ctctatgagt ggctttatgc tctccaacgg ggctgtgcag gtgtttgtag gcgggaaata cgagttacgg tggctggatg acaacagaaa gtttattgtg cgcagtaatg gagtttgtga ggtgcttgat gaggaaaaat tccccctttc ggaggaactc aaccagatgc tctacggtgg cgtgtaa

Details for TrbrB.18664.c.B2.GE37002

HARVESTED ON:	5/21/2013
SEQUENCED ON:	5/23/2013
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	97
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML EACERIGQGS FGTVYRASCD EYPRLALKIT TGKATRLKQE LEVLGRVCTK GKLLLPRFLF GALNKSGDLM AVGMELCFPH TLHDFLLSKC LTDEADKLFV AYQVVQAVAY VHEQHCIHRD VKLQNFVFDL DGNLKLIDFG LATSVWNPPP GDVVAGTIAF MAPEMAHNAL HRDQRVSVGA AADVWSVGMV LFSIFAQRNP YASTGAPRMT ERTADTNMHN TAAIPTDASP NDKQSHEIPX ENAELLRRVA AAEWSWPXGC SVSXKLXGLV DFILVPDPQN RPDIXALLMX PEWG

Validated NT Sequence

attttgttnn nnttaagaag gagatatacc atggctcacc accaccacca ccatatgctg gaggcgtgcg aacgcattgg acaaggttct tttggcaccg tttacagagc ctcttgtgat gaatatccga gattggcatt aaagattaca acaggaaagg ctacacggct caagcaggaa ctggaagtgc ttggaagggt ctgcacaaaa ggaaaactcc tgctgccacg gtttttattt ggtgcattga ataagtccgg ggacctcatg gctgtgggga tggaattgtg tttcccacac acactgcatg acttccttct gtcaaagtgt ctaacagatg aggcggacaa gttgttcgtt gcataccaag tagtgcaagc agtggcgtac gtccatgaac aacattgcat ccacagggat gttaaacttc agaattttgt cttcgatttg gatggtaact taaaactcat agattttgga cttgcgacgt ctgtgtggaa ccctcctcct ggagatgtag tcgctggcac tatcgcattc atggccccgg agatggctca taacgcttta cacagggatc agagagttag cgttggtgcg gcagcagatg tgtggtctgt gggaatggtg cttttttcaa ttttcgcaca acggaatccg tatgcctcca caggtgcccc gcggatgaca gagagaactg ctgacaccaa tatgcataac actgctgcca tacccaccga cgcctctccg aatgacaagc agtcccacga aataccgaan gaaaacgctg agttgcttcg tcgtgtcgct gccgctgaat ggtcgtggcc tantggttgt agcgtctccn ggaagctacn gggacttgtg gacttcatcc ttgtccctga tccacaaaat cgtccagata tcnacgctct cctcatgana ccggaatggg gctaagtgag taananagna tcnnnnnnnn nnnnccccan nnnnnnnnnn ancctnnnnn nnnnnnnnna nntnnnnnnt nnnnnnnngn nntntnnnna nnnnnnnnnn nncnantnnn nnnag

Expected Protein Sequence

MAHHHHHHLE ACERIGQGSF GTVYRASCDE YPRLALKITT GKVTRLKQEL EVLGRVCTKG KLLLPRFLFG ALNKSGDLMA VGMELCFPHT LHDFLLSKCL TDEADKLFVA YQVVQAVAYV HEQHCIHRDV KLQNFVFDLD GNLKLIDFGL ATSVWNPPPG DVVAGTIAFM APEMAHNALH RDQRVSVGAA ADVWSVGMVL FSIFAQRNPY ASTGAPRMTE RTADTNMHNT AAIPTDASPN DKQSHEIPKE NAELLRRVAA AEWSWPSGCS VSRKLRGLVD FVLVPDPQNR PDINALLMRP EWG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctggagg cgtgcgaacg cattggacaa ggttcttttg gcaccgttta cagagcctct tgtgatgaat atccgagatt ggcattaaag attacaacag gaaaggttac acggctcaag caggaactgg aagtgcttgg aagggtctgc acaaaaggaa aactcctgct gccacggttt ttatttggtg cattgaataa gtccggggac ctcatggctg tggggatgga attgtgtttc ccacacacac tgcatgactt ccttctgtca aagtgtctaa cagatgaggc ggacaagttg ttcgttgcat accaagtagt gcaagcagtg gcgtacgtcc atgaacaaca ttgcatccac agggatgtta aacttcagaa ttttgtcttc gatttggatg gtaacttaaa actcatagat tttggacttg cgacgtctgt gtggaaccct cctcctggag atgtagtcgc tggcactatc gcattcatgg ccccggagat ggctcataac gctttacaca gggatcagag agttagcgtt ggtgcggcag cagatgtgtg gtctgtggga atggtgcttt tttcaatttt cgcacaacgg aatccgtatg cctccacagg tgccccgcgg atgacagaga gaactgctga caccaatatg cataacactg ctgccatacc caccgacgcc tctccgaatg acaagcagtc ccacgaaata ccgaaggaaa acgctgagtt gcttcgtcgt gtcgctgccg ctgaatggtc gtggcctagt ggttgtagcg tctccaggaa gctacgggga cttgtggact tcgtccttgt ccctgatcca caaaatcgtc cagatatcaa cgctctcctc atgagaccgg aatggggctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for TrbrB.18664.c.B3.GE37003

HARVESTED ON:	5/21/2013
SEQUENCED ON:	5/23/2013
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	90
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHME LFDREHMVWL EKEQRKSATH PHTFREMGRA NKKYRYGFVC DVCCFEFEPV GSSMYFFHCQ CGRDMCPKCY EEYANNYTCD ACGREFASSG ALRRHSCSCV KRLNDAATVN KPRGRSQGRR SRSVPTEAAR TGVKSRLEVK PVSPKRGRPK RRASVPALER KRSREPAAEV VGIVARRKAE RLSMSKSEVV PPPRRFEGIP RTLSGEWKPM ERLSVGSRSV PPTPXERHML LNGDWIRYYH FYPMEEEGGD SVAVTYHIQP GRTGVTFFNH SFSVHSAVLS VLEXIVYVVD RVDIEXXMTX XYLXLAXIXX XXIYXXXXXX XRXXXXXXXX XX

Validated NT Sequence

ananttttgt ttanctttaa gaaggagata taccatggct caccaccacc accaccatat ggagttattt gatcgagagc atatggtgtg gttggagaaa gagcagcgaa agtccgccac tcacccgcat acattcaggg aaatgggtcg cgcaaacaag aagtacaggt acggcttcgt atgtgatgtc tgctgttttg aattcgaacc agttggatca agtatgtact tctttcactg ccaatgcggt agggacatgt gtccaaaatg ttacgaggag tatgccaata attacacctg cgacgcctgc ggcagggagt ttgccagttc aggtgcgctt cgcaggcaca gttgctcgtg cgtgaagagg ctaaatgatg cagctacagt gaacaagcca agggggagat ctcaggggcg tcgcagtcgt agtgttccaa ccgaagctgc taggactggt gtaaaaagtc ggttggaggt gaaaccagtc agtccgaagc gtggtcggcc gaaacgacga gcatcggtgc cagcactgga acgcaagagg tctcgagagc cggcggctga ggtagtggga attgtggcgc ggaggaaggc agagcgtttg tcaatgagta aatcggaagt tgtaccgccg ccaaggaggt tcgaaggaat accccgcacg ctaagtggtg aatggaaacc gatggagcgt ctctctgtcg ggtctcggag tgtcccaccc acacctgang aacgtcacat gctgcttaac ggtgattgga tacgctacta ccacttttat cctatggagg aagaaggagg cgactcggtc gctgtcacat atcatattca gccgggacgt actggtgtta catttttcaa ccatagtttt tctgtgcact cagctgtgct gtcagtgttg gaacncatcg tatacgtcgt agatcgtgtt gatatcgagg annnaatgac gtnnncgtat ctnncnttgg ccancattna tgnnnnanan atctacgang ncnnnnnnng aancnnncga cnnnnnnann tnaannnnng antannnnnn cg

Expected Protein Sequence

MAHHHHHHEL FDREHMVWLE KEQRKSATHP HTFREMGRAN KKYRYGFVCD VCCFEFEPVG SSMYFFHCQC GRDMCPKCYE EYANNYTCDA CGREFASSGA LRRHSCSCVK RLNDAATVNK PRGRSQGRRS RSVPTEAART GVKSRLEVKP VSPKRGRPKR RASVPALERK RSREPAAEVV GIVARRKAER LSMSKSEVVP PPRRFEGIPR TLSGEWKPME RLSVGSRSVP PTPEERHMLL NGDWIRYYHF YPMEEEGGDS VAVTYHIQPG RTGVTFFNHS FSVHSAVLSV LEHIVYVVDR VDIEEDNDVA RILSLAQALN EEKKIYDVLQ LVETHDTHML K

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgagttat ttgatcgaga gcatatggtg tggttggaga aagagcagcg aaagtccgcc actcacccgc atacattcag ggaaatgggt cgcgcaaaca agaagtacag gtacggcttc gtatgtgatg tctgctgttt tgaattcgaa ccagttggat caagtatgta cttctttcac tgccaatgcg gtagggacat gtgtccaaaa tgttacgagg agtatgccaa taattacacc tgcgacgcct gcggcaggga gtttgccagt tcaggtgcgc ttcgcaggca cagttgctcg tgcgtgaaga ggctaaatga tgcagctaca gtgaacaagc caagggggag atctcagggg cgtcgcagtc gtagtgttcc aaccgaagct gctaggactg gtgtaaaaag tcggttggag gtgaaaccag tcagtccgaa gcgtggtcgg ccgaaacgac gagcatcggt gccagcactg gaacgcaaga ggtctcgaga gccggcggct gaggtagtgg gaattgtggc gcggaggaag gcagagcgtt tgtcaatgag taaatcggaa gttgtaccgc cgccaaggag gttcgaagga ataccccgca cgctaagtgg tgaatggaaa ccgatggagc gtctctctgt cgggtctcgg agtgtcccac ccacacctga ggaacgtcac atgctgctta acggtgattg gatacgctac taccactttt atcctatgga ggaagaagga ggcgactcag tcgctgtcac atatcatatt cagccgggac gtactggtgt tacatttttc aaccatagtt tttctgtgca ctcagctgtg ctgtcagtgt tggaacacat cgtatacgtc gtagatcgtg ttgatatcga ggaagataat gacgtggcgc gtatcttgtc gttggcacaa gcattgaatg aggagaagaa gatctacgat gtccttcaat tggtcgaaac ccacgacaca catatgttaa aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for TrbrB.18664.c.B4.GE43103

HARVESTED ON:	4/19/2019
SEQUENCED ON:	4/23/2019
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMI GTVEQILYEE GCVRAVRVRL PSRDSSQGKC LSLEACERIG QGSFGTVYRA SCDEYPRLAL KITTGKVTRL KQELEVLGRV CTKGKLLLPR FLFGALNKSG DLMAVGMELC FPHTLHDFLL SKCLTDEADK LFVAYQVVQA VAYVHEQHCI HRDVKLQNFV FDLDGNLKLI DFGLATSVWN PPPGDVVAGT IAFMAPEMAH NALHRDQRVS VGAAADVWSV GMVLFSIFAQ RNPYASTGAP RMTERTADTN MHNTAAIPTD ASPNDKQSHE IPKENAELLR RVAAAEWSWP SGCSVSRKLR GLVDFVLVPD PQNRPD

Validated NT Sequence

tatctggacg attttgtgga tcagggacaa ggacgaagtc cacaagtccc cgtagcttcc tggagacgct acaaccacta ggccacgacc attcagcggc agcgacacga cgaagcaact cagcgttttc cttcggtatt tcgtgggact gcttgtcatt cggagaggcg tcggtgggta tggcagcagt gttatgcata ttggtgtcag cagttctctc tgtcatccgc ggggcacctg tagaggcata cggattccgt tgtgcgaaaa ttgaaaagag caccattccc acagaccaca catctgctgc cgcaccaacg ctaactctct gatccctgtg taaagcgtta tgagccatct ccggggccat gaatgcgata gtgccagcga ctacatctcc aggaggaggg ttccacacag acgtcgcaag tccaaaatct atgagtttta agttaccatc caaatcgaag acaaaattct gaagtttaac atccctgtgg atgcaatgtt gttcatggac gtacgccact gcttgcacta cttggtatgc aacgaacaac ttgtccgcct catctgttag acactttgac agaaggaagt catgcagtgt gtgtgggaaa cacaattcca tccccacagc catgaggtcc ccggacttat tcaatgcacc aaataaaaac cgtggcagca ggagtttacc ttttgtgcag acccttccaa gcacttccag ttcctgcttg agccgtgtaa cctttcctgt tgtaatcttt aatgccaatc tcggatattc atcacaagag gctctgtaaa cggtgccaaa agaaccttgt ccaatgcgtt cgcacgcctc cagggataaa cactttcctt gagatgaatc acgagaaggc aatcgcaccc tcacagcacg cacgcagccc tcctcataca atatttgttc tactgtgcca atcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgattg gcacagtaga acaaatattg tacgaggagg gctgcgtgcg tgctgtgagg gtgcgattgc cttctcgtga ttcatctcaa ggaaagtgtt tatccctgga ggcgtgcgaa cgcattggac aaggttcttt tggcaccgtt tacagagcct cttgtgatga atatccgaga ttggcattaa agattacaac aggaaaggtt acacggctca agcaggaact ggaagtgctt ggaagggtct gcacaaaagg aaaactcctg ctgccacggt ttttatttgg tgcattgaat aagtccgggg acctcatggc tgtggggatg gaattgtgtt tcccacacac actgcatgac ttccttctgt caaagtgtct aacagatgag gcggacaagt tgttcgttgc ataccaagta gtgcaagcag tggcgtacgt ccatgaacaa cattgcatcc acagggatgt taaacttcag aattttgtct tcgatttgga tggtaactta aaactcatag attttggact tgcgacgtct gtgtggaacc ctcctcctgg agatgtagtc gctggcacta tcgcattcat ggccccggag atggctcata acgctttaca cagggatcag agagttagcg ttggtgcggc agcagatgtg tggtctgtgg gaatggtgct tttttcaatt ttcgcacaac ggaatccgta tgcctccaca ggtgccccgc ggatgacaga gagaactgct gacaccaata tgcataacac tgctgccata cccaccgacg cctctccgaa tgacaagcag tcccacgaaa taccgaagga aaacgctgag ttgcttcgtc gtgtcgctgc cgctgaatgg tcgtggccta gtggttgtag cgtctccagg aagctacggg gacttgtgga cttcgtcctt gtccctgatc cacaaaatcg tccagatatc aacgctctcc tcatgagacc ggaatggtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at