SSGCID: Target Page

TrbrB.18664.b

Protein kinase, putative

CENTER ID:	TrbrB.18664.b
ORGANISM:	Trypanosoma brucei GUTat10.1
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	AA START	AA STOP
TrbrB.18664.b.B2.GE36999	STPK domain	206	516
TrbrB.18664.b.B3.GE37000	C-terminal domain	524	846
TrbrB.18664.b.B4.GE37001	N-terminal PK-like domain	41	124

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tb927.10.1460
RefSeq:	XP_822404.1
UniProt:	Q38C94

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRSCCSCGKA WCCPERVEDR AEEILKGYVD CFGIDDYRNV QRMAELSGLL SHCSQVDWSN VAGSVSIGLI FRGKHVEDDC DVVVSVLKRP ANKKDELSRY VQKEVGVLAI NFPNIIRVVD YFSVIEGEWA YSMALLKWLG GDGIVEFFTQ SVLGKGRASD VERDDIELRR HPTVDATRIM QGNVEGDYFD SKWRPSTPVR EGNTCVVGDY EMYMDESLGK GQFGEVYRGK HIKEGYHVAM KIIKAPAKKR DETPDEISIM DLLGCPGHPN VVKAYGTYET IDDKNEPSYV LVLELCEGGD LKEYLAKHDP LSEAQARRIM RQLVDCLCFL RERGVMHRDL KPANILLTSC NIDEAVVKVA DWGMAKVNSR KGSALEATEG EEMFESAVGT MAYMAPERLN FDSYDYQAEV WALGVIMYEL LFARHPYIYP KATVCSADAL LGAIARAEQL DMTNRAPSAS TSTTDGAGGE LSQQEGGYVN RRRLSPSCYE LLRQMLHSEA KKRCTIIDVK NHVWFTHEEP RSAPVLVRGG SPRSVLADAL GSASGVTLAG QALAEYPSAV DNSVLPVENC EGGRNVLTTL EKRQTILALR EYIHILQYNI VERERDPGRG LVLLSYGWDL LSAAVNTVFS ECSNVAGDTG GAANATNGPN MSASSSLFHD SLDDLKQVEG YIQTAASQLK QSLQSNQGTQ TTASSAADNE EGVTQHSFTL MTRGTSLDCL PSAKELMLRH AVDLIRWAAS EQLLLTPDDK REKQRGVLEA ELEPKKLQGK KLWEDAMAIL RLLLQQVVVY SPTVTTRSII ADATMGGSVQ VLNVPLVPLT HEEDRRTVQA LLHKVEGLYR NLFVRV

NT Sequence

atgagatctt gctgtagctg tggaaaggcg tggtgttgcc ccgaacgagt tgaggaccgt gccgaagaga ttcttaaggg ctatgttgat tgctttggta tcgatgacta taggaatgtc cagaggatgg cggaactttc tgggcttcta agccattgta gccaggtgga ctggagtaat gtcgccggga gcgtgagcat tggtcttatt ttccgtggaa aacatgtgga agacgactgc gacgttgtgg tatctgtgtt aaagaggcct gcgaacaaga aggacgagtt gagcaggtat gtgcaaaaag aagtgggcgt gttggccata aattttccca atattattag ggttgtggac tattttagtg ttatcgaagg tgagtgggcg tactccatgg ctcttctgaa gtggttgggg ggtgatggta tagtggaatt cttcactcaa agcgtgctag ggaaaggacg ggccagcgac gtggagaggg atgatatcga gctgcgtcga cacccgacag tggatgccac acgtataatg cagggaaatg tagaaggtga ttactttgat agcaaatgga gacctagtac ccctgtacgc gagggcaaca catgtgttgt tggtgactat gaaatgtaca tggatgaaag ccttggaaaa gggcagtttg gagaagtata ccgtgggaag cacataaaag aggggtatca tgttgccatg aagattatta aggcgccagc gaaaaagagg gatgaaacac ccgacgagat cagtataatg gacctgcttg ggtgcccggg tcatccgaac gtcgtcaaag cttacggtac ttacgaaact atagatgaca aaaatgaacc aagctacgtg ctcgttctgg agctctgcga ggggggagac ctaaaagagt acttggccaa gcacgaccca ctatcggagg cgcaagcccg acgtattatg cggcagctgg ttgactgctt gtgtttcctt agggaacgag gtgtgatgca ccgtgattta aagccagcca atattcttct aacatcctgt aatatcgatg aggcggtagt gaaagtagcc gactggggta tggcgaaggt taacagtcga aagggctccg ctttggaggc cacggagggc gaagaaatgt ttgaatctgc tgttggcact atggcttaca tggctccgga gcgcttgaat ttcgattctt atgactatca agcggaggta tgggcactgg gggttatcat gtacgaatta ctgttcgcta gacaccctta tatctacccc aaagcgaccg tctgctcggc ggatgctctg cttggtgcta ttgcgcgtgc agagcagttg gatatgacaa atcgtgcgcc ctctgcatca acttctacga cagacggtgc gggtggggag ctgtcgcaac aggagggagg atacgtgaat cgaaggcgcc tttcgcccag ctgctacgaa ttgcttcggc agatgctgca ttccgaggcg aaaaagcgct gcacaatcat cgatgtgaag aaccacgtgt ggtttactca tgaagagcct cgaagtgcac ctgtattagt gagaggagga tcaccgaggt ccgtacttgc tgatgctctg gggtctgcaa gcggggtgac acttgcggga caagcgctgg ctgagtaccc cagcgcagtg gataattcag ttctcccggt tgagaattgt gaaggcggta gaaatgtttt aacgactttg gagaagaggc aaactatctt ggcgcttcgt gagtatattc atattctgca atataacatt gtagagaggg agcgtgaccc aggccgaggc cttgttctgc tatcctacgg gtgggatctt cttagtgcgg cagtaaatac cgtattttcg gagtgcagca atgttgccgg ggatacggga ggagctgcca acgccacgaa cggtccaaac atgagtgcct catcgtctct ctttcatgac tccttggatg acctcaagca ggtagaaggg tacattcaaa cagccgcgtc tcagctcaaa cagagccttc agagtaacca aggtactcaa acaacagcaa gttcagcggc ggacaacgag gagggagtta cacagcactc cttcacgtta atgacgcgag gtacttcgct tgattgctta ccttccgcga aggagttgat gctccggcac gctgttgatc tcatccgctg ggcggcttct gaacagcttt tgctgacccc ggatgacaaa cgcgaaaaac agaggggggt gttagaagct gaactggagc caaagaagtt acagggaaag aaactatggg aggacgcaat ggccattttg cgtctgctgt tgcagcaggt ggtagtttac tcaccgacgg tgaccacccg gtccatcatc gctgatgcca ctatgggtgg ctctgtgcaa gtgcttaacg tacctctcgt tccactcact cacgaggagg acaggcgcac cgtccaggct ttattgcaca aagtggaggg gctctaccga aacctattcg tcagagtgta g

Details for TrbrB.18664.b.B2.GE36999

HARVESTED ON:	5/21/2013
SEQUENCED ON:	5/23/2013
EXPECTED MW:	36kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV VGDYEMYMDE SLGKGQFGEV YRGKHIKEGY HVAMKIIKAP AKKRDETPDE ISIMDLLGCP GHPNVVKAYG TYETIDDKNE PSYVLVLELC EGGDLKEYLA KHDPLSEAQA RRIMRQLVDC LCFLRERGVM HRDLKPANIL LTSCNIDEAV VKVADWGMAK VNSRKGSALE ATEGEEMFES AVGTMAYMAP ERLNFDSYDY QAEVWALGVI MYELLFARHP YIYPKATVCS ADALLGAIAR AEQLDMTNRA SSASTSTTDG AGEELSQQEE GYVNRRRLSP SCYELLRQML HSEAKKRCTI IDVKNHVWFT

Validated NT Sequence

tactcactta agtaaaccac acgtggttct tcacatcgat gattgtgcag cgctttttcg cctcggaatg cagcatctgc cgaagcaatt cgtagcagct gggcgaaagg cgccttcgat tcacgtatcc ttcctcctgt tgcgacagct cctcacccgc accgtctgtc gtagaagttg atgcagagga cgcacgattt gtcatatcca actgctctgc acgcgcaata gcaccaagca gagcatccgc cgagcagacg gtcgctttgg ggtagatata agggtgtcta gcaaacagta attcgtacat gataaccccc agtgcccata cctccgcttg atagtcataa gaatcgaaat tcaagcgctc cggagccatg taagccatag tgccaacagc agattcaaac atttcttcgc cctccgtggc ctccagagcg gagccctttc gactgttaac cttcgccata ccccagtcgg ctactttcac taccgcctca tcgatattac aggatgttag aagaatattg gctggcttta aatcacggtg catcacacct cgttccctaa ggaaacacaa gcagtcaacc agctgccgca taatacgtcg ggcttgcgcc tccgatagtg ggtcgtgctt ggccaagtac tcttttaggt ctcccccctc gcagagctcc agaacgagca cgtagcttgg ttcatttttg tcatctatag tttcgtaagt accgtaagct ttgacgacgt tcggatgacc cgggcaccca agcaggtcca ttatactgat ctcgtcgggt gtttcatccc tctttttcgc tggcgcctta ataatcttca tggcaacatg atacccctct tttatgtgct tcccacggta tacttctcca aactgccctt ttccaaggct ttcatccatg tacatttcat agtcaccaac aaccatatgg tggtggtggt ggtgagccat ggtatntctc cttcttaaa

Expected Protein Sequence

MAHHHHHHVV GDYEMYMDES LGKGQFGEVY RGKHIKEGYH VAMKIIKAPA KKRDETPDEI SIMDLLGCPG HPNVVKAYGT YETIDDKNEP SYVLVLELCE GGDLKEYLAK HDPLSEAQAR RIMRQLVDCL CFLRERGVMH RDLKPANILL TSCNIDEAVV KVADWGMAKV NSRKGSALEA TEGEEMFESA VGTMAYMAPE RLNFDSYDYQ AEVWALGVIM YELLFARHPY IYPKATVCSA DALLGAIARA EQLDMTNRAP SASTSTTDGA GGELSQQEGG YVNRRRLSPS CYELLRQMLH SEAKKRCTII DVKNHVWFT

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgttgttg gtgactatga aatgtacatg gatgaaagcc ttggaaaagg gcagtttgga gaagtatacc gtgggaagca cataaaagag gggtatcatg ttgccatgaa gattattaag gcgccagcga aaaagaggga tgaaacaccc gacgagatca gtataatgga cctgcttggg tgcccgggtc atccgaacgt cgtcaaagct tacggtactt acgaaactat agatgacaaa aatgaaccaa gctacgtgct cgttctggag ctctgcgagg ggggagacct aaaagagtac ttggccaagc acgacccact atcggaggcg caagcccgac gtattatgcg gcagctggtt gactgcttgt gtttccttag ggaacgaggt gtgatgcacc gtgatttaaa gccagccaat attcttctaa catcctgtaa tatcgatgag gcggtagtga aagtagccga ctggggtatg gcgaaggtta acagtcgaaa gggctccgct ttggaggcca cggagggcga agaaatgttt gaatctgctg ttggcactat ggcttacatg gctccggagc gcttgaattt cgattcttat gactatcaag cggaggtatg ggcactgggg gttatcatgt acgaattact gttcgctaga cacccttata tctaccccaa agcgaccgtc tgctcggcgg atgctctgct tggtgctatt gcgcgtgcag agcagttgga tatgacaaat cgtgcgccct ctgcatcaac ttctacgaca gacggtgcgg gtggggagct gtcgcaacag gagggaggat acgtgaatcg aaggcgcctt tcgcccagct gctacgaatt gcttcggcag atgctgcatt ccgaggcgaa aaagcgctgc acaatcatcg atgtgaagaa ccacgtgtgg tttacttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for TrbrB.18664.b.B3.GE37000

HARVESTED ON:	5/21/2013
SEQUENCED ON:	10/23/2013
EXPECTED MW:	36kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MPVLVRGGSP RSVLADALGS ASGVTLAGQA LAEYPSAVDN SVLPVENCEG GRNVLTTLEK RQTILALREY IHILQYNIVE RERDPGRGLV LLSYGWDLLS AAVNTVFSEC SNVAGDTGGA ANATNGPNMS ASSSLFHDSL DDLKQVEGYI QTAASQLKQS LQSNQGTQTT ASSAADNEEG VTQHSFTLMT RGTSLDCLPS AKELMLRHAV DLIRWAASEQ LMLTPDDKRE KQRGVLEAEL EPKKLQGKKL WEDAMAILRL LLQQVVVYSP TVTTRSIIAD ATMGGSVQVL NVPLVPLTHE EDRRTVQALL HKVEGLYRNL FVRV

Validated NT Sequence

caccaccacc atatgcctgt attagtgaga ggaggatcac cgaggtccgt acttgctgat gctctggggt ctgcaagcgg ggtgacactt gcaggacaag cgctggctga gtaccccagt gcagtggata attcagttct cccggttgag aattgtgaag gcggtagaaa tgttttaacg actttggaga agaggcaaac tatcttggcg cttcgtgagt atattcatat tctgcaatat aacattgtag agagggagcg tgacccaggc cgaggtcttg ttctgctatc ctacgggtgg gatcttctta gtgcggcagt aaataccgta ttttcggagt gcagcaatgt tgccggggat acgggaggag ctgccaacgc cacgaacggt ccaaacatga gtgcctcatc gtctctcttt catgactcct tggatgacct caagcaggta gaagggtaca ttcaaacagc cgcgtctcag ctcaaacaga gccttcagag taaccaaggt actcaaacaa cagcaagttc agcggcggac aacgaggagg gagttacaca gcactccttc acgttaatga cgcgaggtac ttcgcttgat tgcttacctt ccgcgaagga gttgatgctc cggcacgctg ttgatctcat ccgctgggcg gcttctgaac agcttatgct gaccccggat gacaaacgcg aaaaacagag gggggtgtta gaagctgaac tggagccaaa gaagttacag ggaaagaaac tatgggagga cgcaatggcc attttgcgtc tgctgttgca gcaggtggta gtttactcac cgacggtgac cacccggtcc atcatcgctg atgccactat gggtggctct gtgcaagtgc ttaacgtacc tctcgttcca ctcactcacg aggaggacag gcgcaccgtc caggctttat tgcacaaagt ggaggggctc taccgaaacc tattcgtcag agtgtaagtg agtaaga

Expected Protein Sequence

MAHHHHHHPV LVRGGSPRSV LADALGSASG VTLAGQALAE YPSAVDNSVL PVENCEGGRN VLTTLEKRQT ILALREYIHI LQYNIVERER DPGRGLVLLS YGWDLLSAAV NTVFSECSNV AGDTGGAANA TNGPNMSASS SLFHDSLDDL KQVEGYIQTA ASQLKQSLQS NQGTQTTASS AADNEEGVTQ HSFTLMTRGT SLDCLPSAKE LMLRHAVDLI RWAASEQLLL TPDDKREKQR GVLEAELEPK KLQGKKLWED AMAILRLLLQ QVVVYSPTVT TRSIIADATM GGSVQVLNVP LVPLTHEEDR RTVQALLHKV EGLYRNLFVR V

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcctgtat tagtgagagg aggatcaccg aggtccgtac ttgctgatgc tctggggtct gcaagcgggg tgacacttgc gggacaagcg ctggctgagt accccagcgc agtggataat tcagttctcc cggttgagaa ttgtgaaggc ggtagaaatg ttttaacgac tttggagaag aggcaaacta tcttggcgct tcgtgagtat attcatattc tgcaatataa cattgtagag agggagcgtg acccaggccg aggccttgtt ctgctatcct acgggtggga tcttcttagt gcggcagtaa ataccgtatt ttcggagtgc agcaatgttg ccggggatac gggaggagct gccaacgcca cgaacggtcc aaacatgagt gcctcatcgt ctctctttca tgactccttg gatgacctca agcaggtaga agggtacatt caaacagccg cgtctcagct caaacagagc cttcagagta accaaggtac tcaaacaaca gcaagttcag cggcggacaa cgaggaggga gttacacagc actccttcac gttaatgacg cgaggtactt cgcttgattg cttaccttcc gcgaaggagt tgatgctccg gcacgctgtt gatctcatcc gctgggcggc ttctgaacag cttttgctga ccccggatga caaacgcgaa aaacagaggg gggtgttaga agctgaactg gagccaaaga agttacaggg aaagaaacta tgggaggacg caatggccat tttgcgtctg ctgttgcagc aggtggtagt ttactcaccg acggtgacca cccggtccat catcgctgat gccactatgg gtggctctgt gcaagtgctt aacgtacctc tcgttccact cactcacgag gaggacaggc gcaccgtcca ggctttattg cacaaagtgg aggggctcta ccgaaaccta ttcgtcagag tgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for TrbrB.18664.b.B4.GE37001

HARVESTED ON:	data unavailable
SEQUENCED ON:	5/23/2013
EXPECTED MW:	10kDa
OBSERVED MW:	7kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	97
PERCENT COVERAGE:	101

Validated AA Sequence

MAHHHHHHMQ RMAELSGLLS HCSQVDWSYV AGSVNIGLIF RGKHVEDDCD VVVSVLKRPA NKKDELSRYV QKEVGVLAIN FPNIIRVVDY FSV

Validated NT Sequence

ttntnnnntt taagaaggag atataccatg gctcaccacc accaccacca tatgcagagg atggcggaac tttctgggct tctaagccat tgtagccagg tggactggag ttatgtcgcc gggagcgtga acattggtct tattttccgt ggaaaacatg tggaagacga ctgcgacgtt gtggtatctg tgttaaagag gcctgcgaac aagaaggacg agctgagcag gtatgtgcaa aaagaagtgg gggtgttggc cataaatttt cccaatatta ttagggttgt ggactatttt agtgtttaag tgagtaagat aggattcggc tgctaacnnn ncccgaaaga nnnnnnnnnn nnnnnnggng nnnncttttc nnnncntnnn nnatgttnnt gnnnnngnan ngnnnnnnga nnncnngngn tagnnnnnnn nnnnncnggn nctnnnnnnn cannnnnnnc gnnnntncna nnnntacntn gagncngngt gnnnnannnn atttgcaann nnnnncctan ncnntgacnn cncnngtgnn tnnnnnanct tcgnnttnnn tnnnntngcn nnnnnnnnat nncnccnnan gatttnncnn actgnggcnn tacnngctgn tncnnnnnnc nnnannnnnn ccacannann nnntnctacn atancgntgn nntncnnnca nnnnnnngnn ntannnnnna nntgnannan cgcngcncnc nnnncnnnnn nnnnnncgnn nnancnnncg cnncncnnnn nnnntatngn nnnnnnnnnn nnnnnnnnct nnnnannncn nannnntnnc aacttnntnn tggcnannnn ncatagccna nnnnnnnccc nngntnantn catnnnnnnn tngnctacnn nannnnannn nnnnnnncnc cnnggnnnnn ntacannnnn naattncnnn nccctcnttc ngnnnnnnnn gangaa

Expected Protein Sequence

MAHHHHHHQR MAELSGLLSH CSQVDWSNVA GSVSIGLIFR GKHVEDDCDV VVSVLKRPAN KKDELSRYVQ KEVGVLAINF PNIIRVVDYF SV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcagagga tggcggaact ttctgggctt ctaagccatt gtagccaggt ggactggagt aatgtcgccg ggagcgtgag cattggtctt attttccgtg gaaaacatgt ggaagacgac tgcgacgttg tggtatctgt gttaaagagg cctgcgaaca agaaggacga gttgagcagg tatgtgcaaa aagaagtggg cgtgttggcc ataaattttc ccaatattat tagggttgtg gactatttta gtgtttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat