SSGCID: Target Page

TrbrB.17193.a

Receptor for activated protein kinase C (TRACK); guanine nucleotide-binding protein subunit beta-like protein

CENTER ID:	TrbrB.17193.a
ORGANISM:	Trypanosoma brucei GUTat10.1
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrB.17193.a.B11.GE39847	full length		1	318

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrB.17193.a.B11.PW37898	full length		1	318

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tb927.11.11360
UniProt:	P69103

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAVAYEGQLT GHRGWVTSLA CPQTPETATK VVSTSRDKTL LSWGPNPDRH SSECSYGLPD RRLEGHSAFV SDVALSNNGN FAVSASWDHS LRLWNLQNGQ CQYKFLGHTK DVLSVAFSPD NRQIVSGGRD NALRVWNVKG ECMHTLSRGA HTDWVSCVRF SPSLDAPVIV SGGWDNLVKV WDLATGRLVT DLKGHTNYVT SVTVSPDGSL CASSDKDGVA RLWDLTKGEA LSEMAAGAPI NQICFSPNRY WMCAATEKGI RIFDLENKDI IVELAPEHQG SKKIVPECVS IAWSADGSTL YSGYTDNVIR VWGVSENA

NT Sequence

atggctgtcg cttacgaagg acaactgacg ggtcaccgtg gctgggttac atccctcgcc tgcccacaaa cccctgaaac cgcaacgaag gtggtatcca catcccggga caagaccctt ctttcatggg gccctaaccc cgaccggcac agcagtgagt gcagctatgg ccttcccgac aggcgtctgg aggggcacag cgcatttgtg tctgacgttg ccctttccaa caacgggaat ttcgctgtgt ctgcgtcatg ggaccattcc ctgcgcttgt ggaacctgca gaacggacaa tgtcaataca agtttcttgg ccacacaaag gacgtacttt ccgtggcctt ctctcccgac aaccgacaga ttgtctccgg tggtcgtgac aacgctctgc gcgtatggaa tgtcaaaggc gaatgcatgc acactctgag ccgcggtgct cacaccgact gggtgagttg tgtccgcttc tccccgtctt tggatgcccc tgtcatcgtc tctggtggtt gggacaacct ggtgaaggtc tgggacctcg ctaccgggcg acttgtcacg gaccttaagg gacatacaaa ctatgtcaca tctgtgaccg tttcccctga cggttccctc tgtgcatctt ccgacaagga cggcgtcgct cgtctgtggg acctgactaa gggtgaggcc ctttctgaga tggccgctgg tgcgcctatc aaccaaatct gcttctcccc caaccgttat tggatgtgtg cggctaccga aaagggcatc cgcatctttg acctggagaa caaggacatc atcgtcgagc ttgcgccgga gcatcagggc agcaaaaaga ttgtcccgga gtgcgtttcc atcgcgtggt ctgcggatgg tagcaccctg tactctgggt acacggacaa tgtcatccgt gtttggggtg tatcggagaa cgcgtga

Details for TrbrB.17193.a.B11.GE39847

HARVESTED ON:	2/4/2016
SEQUENCED ON:	1/27/2016
EXPECTED MW:	36kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMA VAYEGQLTGH RGWVTSLACP QTPETATKVV STSRDKTLLS WGPNPDRHSS ECSYGLPDRR LEGHSAFVSD VALSNNGNFA VSASWDHSLR LWNLQNGQCQ YKFLGHTKDV LSVAFSPDNR QIVSGGRDNA LRVWNVKGEC MHTLSRGAHT DWVSCVRFSP SLDAPVIVSG GWDNLVKVWD LATGRLVTDL KGHTNYVTSV TVSPDGSLCA SSDKDGVARL WDLTKGEALS EMAAGAPINQ ICFSPNRYWM CAATEKGIRI FDLENKDIIV ELAPEHQGSK KIVPECVSIA WSADGSTLYS GYTDNVI

Validated NT Sequence

ggatgacatt gtccgtgtac ccagagtaca gggtgctacc atccgcagac cacgcgatgg aaacgcactc cgggacaatc tttttgctgc cctgatgctc cggcgcaagc tcgacgatga tgtccttgtt ctccaggtca aagatgcgga tgcccttttc ggtagccgca cacatccaat aacggttggg agagaagcag atttggttga taggcgcacc agcggccatc tcagaaaggg cctcaccctt agtcaggtcc cacagacgag cgacgccgtc cttgtcggaa gatgcacaga gggaaccgtc aggggaaacg gtcacagatg tgacatagtt tgtatgtccc ttaaggtccg tgacaagtcg cccggtagcg aggtcccaga ccttcaccag gttgtcccaa ccaccagaga cgatgacagg ggcatccaaa gacggggaga agcggacaca actcacccag tcggtgtgag caccgcggct cagagtgtgc atgcattcgc ccttgacatt ccatacgcgc agagcgttgt cacgaccacc ggagacaatc tgtcggttgt cgggagagaa ggccacggaa agtacgtcct ttgtgtggcc aagaaacttg tattgacatt gtccgttctg caggttccac aagcgcaggg aatggtccca tgacgcagac acagcgaaat tcccgttgtt ggaaagggca acgtcagaca caaatgcgct gtgcccctcc agacgcctgt cgggaaggcc atagctgcac tcactgctgt gccggtcagg gttagggccc catgaaagaa gggtcttgtc ccgggacgtg gataccacct tcgttgcggt ttcaggggtt tgtgggcagg cgagggatgt aacccagcca cggtgacccg tcagttgtcc ttcgtaagcg acagccatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctg tcgcttacga aggacaactg acgggtcacc gtggctgggt tacatccctc gcctgcccac aaacccctga aaccgcaacg aaggtggtat ccacatcccg ggacaagacc cttctttcat ggggccctaa ccccgaccgg cacagcagtg agtgcagcta tggccttccc gacaggcgtc tggaggggca cagcgcattt gtgtctgacg ttgccctttc caacaacggg aatttcgctg tgtctgcgtc atgggaccat tccctgcgct tgtggaacct gcagaacgga caatgtcaat acaagtttct tggccacaca aaggacgtac tttccgtggc cttctctccc gacaaccgac agattgtctc cggtggtcgt gacaacgctc tgcgcgtatg gaatgtcaaa ggcgaatgca tgcacactct gagccgcggt gctcacaccg actgggtgag ttgtgtccgc ttctccccgt ctttggatgc ccctgtcatc gtctctggtg gttgggacaa cctggtgaag gtctgggacc tcgctaccgg gcgacttgtc acggacctta agggacatac aaactatgtc acatctgtga ccgtttcccc tgacggttcc ctctgtgcat cttccgacaa ggacggcgtc gctcgtctgt gggacctgac taagggtgag gccctttctg agatggccgc tggtgcgcct atcaaccaaa tctgcttctc ccccaaccgt tattggatgt gtgcggctac cgaaaagggc atccgcatct ttgacctgga gaacaaggac atcatcgtcg agcttgcgcc ggagcatcag ggcagcaaaa agattgtccc ggagtgcgtt tccatcgcgt ggtctgcgga tggtagcacc ctgtactctg ggtacacgga caatgtcatc cgtgtttggg gtgtatcgga gaacgcgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for TrbrB.17193.a.B11.PW37898

PURIFICATION DATe:	4/27/2016
CONCENTRATION:	24mg/ml
OBSERVED MW:	33kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCTG TCGCTTACGA AGGACAACTG ACGGGTCACC GTGGCTGGGT TACATCCCTC GCCTGCCCAC AAACCCCTGA AACCGCAACG AAGGTGGTAT CCACATCCCG GGACAAGACC CTTCTTTCAT GGGGCCCTAA CCCCGACCGG CACAGCAGTG AGTGCAGCTA TGGCCTTCCC GACAGGCGTC TGGAGGGGCA CAGCGCATTT GTGTCTGACG TTGCCCTTTC CAACAACGGG AATTTCGCTG TGTCTGCGTC ATGGGACCAT TCCCTGCGCT TGTGGAACCT GCAGAACGGA CAATGTCAAT ACAAGTTTCT TGGCCACACA AAGGACGTAC TTTCCGTGGC CTTCTCTCCC GACAACCGAC AGATTGTCTC CGGTGGTCGT GACAACGCTC TGCGCGTATG GAATGTCAAA GGCGAATGCA TGCACACTCT GAGCCGCGGT GCTCACACCG ACTGGGTGAG TTGTGTCCGC TTCTCCCCGT CTTTGGATGC CCCTGTCATC GTCTCTGGTG GTTGGGACAA CCTGGTGAAG GTCTGGGACC TCGCTACCGG GCGACTTGTC ACGGACCTTA AGGGACATAC AAACTATGTC ACATCTGTGA CCGTTTCCCC TGACGGTTCC CTCTGTGCAT CTTCCGACAA GGACGGCGTC GCTCGTCTGT GGGACCTGAC TAAGGGTGAG GCCCTTTCTG AGATGGCCGC TGGTGCGCCT ATCAACCAAA TCTGCTTCTC CCCCAACCGT TATTGGATGT GTGCGGCTAC CGAAAAGGGC ATCCGCATCT TTGACCTGGA GAACAAGGAC ATCATCGTCG AGCTTGCGCC GGAGCATCAG GGCAGCAAAA AGATTGTCCC GGAGTGCGTT TCCATCGCGT GGTCTGCGGA TGGTAGCACC CTGTACTCTG GGTACACGGA CAATGTCATC CGTGTTTGGG GTGTATCGGA GAACGCGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT