TrbrB.01593.a

Protein kinase, putative

CENTER ID: TrbrB.01593.a
ORGANISM: Trypanosoma brucei GUTat10.1
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrB.01593.a.B1.GE36997 full length 1 373
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tb927.10.3230
RefSeq: XP_822575.1
UniProt: Q38BS3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNSNAPLIFD VPPVFKVESI IGQGSYGVVC KALYGIDQIA IKKIPNYTKS DETARRVLRE IEILQNLQFC EQVVECRLLF RPTSREKDVY VVMNFLPSDL SSVIKNKSVP LNEKIICYIT CQLLLALRAM HRCNVLHRDL SPRNILVNHD SQVFVCDFGL SRFFDPDEQL SFGVVTQWYR APEIILDAEY DAANDIWGVG VIVCELVLRR HLFPGKWNDS ADQLNHIFHL VGTPPASVFE QGQPFARASA NARKYALSVV EKKPCSPMLQ ELLRSSPLFQ SNEVESHSVI DLAVKLLSFN PRDRPTADDA LRHPWFDQFR SFIGEMIETQ DGHGVGTMQL SACGTLPVED VVKRIEELVP IFSQDLLVSD DED
NT Sequence
atgaacagta atgctccgct tatctttgat gtgcctcctg tgtttaaggt ggagtccata attgggcaag gctcatacgg tgtggtttgc aaggcgttgt atggaattga tcaaattgcc atcaagaaaa taccgaacta cacgaaaagt gacgagaccg cgcggcgtgt gttacgtgaa atagaaattt tacagaactt acagttctgc gagcaggtcg tagagtgccg acttcttttt cggccgacta gtcgtgaaaa ggatgtgtat gtcgtaatga actttcttcc ttctgacctc tcctccgtta tcaagaacaa atccgtcccg ctcaatgaga aaattatctg ctacattaca tgtcaacttt tactagctct tcgtgccatg caccgctgta atgtacttca ccgtgacttg tctccacgta acatcctcgt caatcacgat tcgcaggtat ttgtgtgtga tttcggtctc agtcgtttct ttgatcccga cgagcaattg tcatttggtg ttgtaacgca gtggtatcgg gcgccagaga ttattttgga tgccgagtac gacgctgcca atgatatttg gggcgttggc gttatcgtgt gtgagttggt gctcagacgt catcttttcc ctggaaagtg gaacgactct gcggatcaac ttaatcacat cttccacctt gttggcaccc cgccagcatc tgtgtttgag caggggcaac cgtttgctag ggcttctgcg aatgcaagaa aatatgctct ttctgttgtg gagaagaaac cgtgctctcc gatgttgcag gagttgctga gaagttcacc actttttcag agcaatgagg tggagtcgca ttcggtgatt gaccttgccg taaagcttct ttcatttaac ccacgtgaca gaccgacagc tgatgatgcg ttgcgtcacc catggtttga tcagtttcgc tccttcattg gtgaaatgat tgagactcag gacggtcacg gtgtggggac tatgcaactt tcagcctgtg gtacacttcc tgtggaggat gtggtgaaga ggatcgagga actcgttcca attttctcac aagacctact agttagcgat gatgaggatt ga
Details for TrbrB.01593.a.B1.GE36997
HARVESTED ON: 5/21/2013
SEQUENCED ON: 5/23/2013
EXPECTED MW: 43kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 70
Validated AA Sequence
MNFLPSDLSS VIKNKSVPLN EKIICYITCQ LLLALRAMHR CNVLHRDLSP RNILVNHDSQ VFVCDFGLSR FFDPDEQLSF GVVTQWYRAP EIILDAEYDA ANDIWGVGVI VCELVLRRHL FPGKWNDSAD QLNHIFHLVG TPPASVFEQG QPFARASANA RKYALSVVEK KPCSPMLQEL LRSSPLFQSN EVESHLVIDL AVKLLSFNPR DRPTADDALR HPWFDQFRSF IGEMIETQDG HGVGTMQLSV CGTLPVEDVV KRIEELVPIF
Validated NT Sequence
gagaaaattg gaacgagttc ctcgatcctc ttcaccacat cctccacagg gagtgtacca cagactgaaa gttgcatagt tcccacaccg tgcccgtcct gagtctcaat catttcacca atgaaggagc gaaactgatc aaaccatggg tgacgcaacg catcatcagc tgtcggtctg tcacgtgggt taaatgaaag aagctttacg gcaaggtcaa tcaccaaatg cgactccacc tcattgctct gaaaaagtgg tgaacttctc agcaactcct gcaacatcgg agagcacggt ttcttctcca caacagaaag agcatatttt cttgcattcg cagaagccct agcaaacggt tgcccctgct caaacacaga tgctggcggg gtgccaacaa ggtggaagat gtgattaagt tgatccgcag agtcgttcca ctttccaggg aaaagatgac gtctgagcac caactcacac acgataacgc caacgcccca aatatcattg gcagcgtcgt actcggcatc caaaataatc tctggcgccc gataccactg cgttacaaca ccaaatgaca attgctcgtc ggggtcaaag aaacgactga gaccgaaatc acacacaaat acctgcgaat cgtgattgac gaggatgtta cgtggagaca agtcacggtg aagtacatta cagcggtgca tggcacgaag agctagtaaa agttgacatg taatgtagca gataattttc tcattgagcg ggacggattt gttcttgata acggaggaga ggtcagaagg aagaaagttc attacgacat acacatcctt ttcacgacta gtcggccgaa aaagaagtcg gcactctacg acctgctcgc agaactgtaa gttctgtaaa atttctattt cacgtaacac acgccgcgcg gtctcgtcac ttttcgtgta gttcggtatt ttcttgatgg caatttgatc aattccatac aacgccttgc aaaccacacc gtatgagcct tgcccaatta tggactccac cttaaacaca ggaggcacat caaagataag cggagcatta ct
Expected Protein Sequence
MAHHHHHHMN SNAPLIFDVP PVFKVESIIG QGSYGVVCKA LYGIDQIAIK KIPNYTKSDE TARRVLREIE ILQNLQFCEQ VVECRLLFRP TSREKDVYVV MNFLPSDLSS VIKNKSVPLN EKIICYITCQ LLLALRAMHR CNVLHRDLSP RNILVNHDSQ VFVCDFGLSR FFDPDEQLSF GVVTQWYRAP EIILDAEYDA ANDIWGVGVI VCELVLRRHL FPGKWNDSAD QLNHIFHLVG TPPASVFEQG QPFARASANA RKYALSVVEK KPCSPMLQEL LRSSPLFQSN EVESHSVIDL AVKLLSFNPR DRPTADDALR HPWFDQFRSF IGEMIETQDG HGVGTMQLSA CGTLPVEDVV KRIEELVPIF SQDLLVSDDE D
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaca gtaatgctcc gcttatcttt gatgtgcctc ctgtgtttaa ggtggagtcc ataattgggc aaggctcata cggtgtggtt tgcaaggcgt tgtatggaat tgatcaaatt gccatcaaga aaataccgaa ctacacgaaa agtgacgaga ccgcgcggcg tgtgttacgt gaaatagaaa ttttacagaa cttacagttc tgcgagcagg tcgtagagtg ccgacttctt tttcggccga ctagtcgtga aaaggatgtg tatgtcgtaa tgaactttct tccttctgac ctctcctccg ttatcaagaa caaatccgtc ccgctcaatg agaaaattat ctgctacatt acatgtcaac ttttactagc tcttcgtgcc atgcaccgct gtaatgtact tcaccgtgac ttgtctccac gtaacatcct cgtcaatcac gattcgcagg tatttgtgtg tgatttcggt ctcagtcgtt tctttgatcc cgacgagcaa ttgtcatttg gtgttgtaac gcagtggtat cgggcgccag agattatttt ggatgccgag tacgacgctg ccaatgatat ttggggcgtt ggcgttatcg tgtgtgagtt ggtgctcaga cgtcatcttt tccctggaaa gtggaacgac tctgcggatc aacttaatca catcttccac cttgttggca ccccgccagc atctgtgttt gagcaggggc aaccgtttgc tagggcttct gcgaatgcaa gaaaatatgc tctttctgtt gtggagaaga aaccgtgctc tccgatgttg caggagttgc tgagaagttc accacttttt cagagcaatg aggtggagtc gcattcggtg attgaccttg ccgtaaagct tctttcattt aacccacgtg acagaccgac agctgatgat gcgttgcgtc acccatggtt tgatcagttt cgctccttca ttggtgaaat gattgagact caggacggtc acggtgtggg gactatgcaa ctttcagcct gtggtacact tcctgtggag gatgtggtga agaggatcga ggaactcgtt ccaattttct cacaagacct actagttagc gatgatgagg attgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat