SSGCID: Target Page

TrbrB.01480.c

Protein kinase, putative

CENTER ID:	TrbrB.01480.c
ORGANISM:	Trypanosoma brucei GUTat10.1
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrB.01480.c.B1.GE36996	full length		1	473

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tb927.8.5730
RefSeq:	XP_847403.1
UniProt:	Q57X09

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSDEGFEDLF ILDTDPEELY EELGPIGVGN YGSVIKARNR HTQEITAIKL VLLVDKDERD FVQREISILK HCRHPNIVHY YGAHKSLSKL WIVMEYCEGG SIDMVYKVLR HPLPEPLIAY VCRQVLLGLQ YLHANRKIHR DIKGGNILLT RDGGVKLADF GVSTELSHSL SRRNTFVGTL LWMAPEAILE LDYDEKADLW SLGITLIEMA EGAPPYIDTH PARALFMIPK MDPPSLQDKE RWSPQMTLFL KRLLTKDHNA RPTATTMLND PFVLPENIGT QEQLKAVIDE VLAKKASIDP QRMRDYTDSS ATFVERTSSD GGGGDDHSTD SRDEVQPHLT AKGLTNAPAN SIEALDGAGT HLFFQDGTLL PLPMLYTQDI SFDELCVGEE LNGPFSLPVP SVESLLVPPE QQSTACHGGE ALAHTMHVTA KLKEMLYYHK QLPYCRGLTD AEAAKSREMQ LKYGSVLKSV YRL

NT Sequence

atgagcgacg aggggtttga agatctcttt atattggaca ccgacccgga ggagttgtac gaagagttgg gccccattgg tgtagggaac tatggatctg ttataaaggc acggaaccgt catacgcagg aaatcactgc catcaagctc gtgttgttgg ttgacaaaga tgagcgggac ttcgtgcagc gggagattag catattaaag cattgtaggc acccgaacat tgtgcactac tatggtgctc acaagtctct tagtaagtta tggattgtaa tggagtactg tgaaggtgga tccattgata tggtttataa ggtgttgcgg caccccttgc cagagccact gattgcctat gtttgccgtc aagtgttgct tgggctgcaa tacctccacg caaatcgaaa gatacaccga gacatcaagg gtggtaacat tttgctgacg cgtgatggtg gagtgaaact cgctgatttt ggtgtaagca cggagttgtc ccactccctc tcgcgtcgta acacatttgt gggcacgtta ttgtggatgg cgccggaggc cattttggag ctcgattatg acgagaaagc tgatctgtgg agtttaggta ttacattgat tgaaatggcg gagggcgcac caccgtatat tgacactcac ccagcgcgtg ccttgtttat gattccgaag atggatccac cctcgctcca ggacaaggaa cggtggtcgc cacaaatgac actttttctc aaaaggttac tgacaaagga tcacaacgct cgcccgacag ccacgacaat gcttaacgac ccctttgtac taccggagaa tattggtacg caggagcagc ttaaagccgt tatagatgag gtgcttgcta agaaagcttc aatagacccg caacgtatgc gcgactacac ggattcgagc gcaacattcg tggaaaggac gtcttccgat ggtggaggtg gggatgatca cagcacggac agcagggatg aggttcagcc gcacttaaca gcaaagggcc tcaccaacgc tcccgcgaat tcgatagagg cgttggacgg ggccggaact cacctcttct tccaggatgg tacactccta ccactgccta tgctctacac acaggacata agtttcgatg aactttgtgt tggtgaggag ctcaatgggc cattttcatt accagtgcct agtgtggagt cgttgttggt tccccctgaa cagcagtcaa cggcttgtca cggcggcgag gcgctggcgc acaccatgca cgtaacggcg aagctgaagg agatgctgta ttaccacaag cagcttcctt actgtcgcgg tctcacagac gcggaggcag cgaagtccag ggaaatgcag ctaaagtatg ggtctgtgtt gaagagtgtc taccgcctgt ag

Details for TrbrB.01480.c.B1.GE36996

HARVESTED ON:	5/21/2013
SEQUENCED ON:	5/23/2013
EXPECTED MW:	54kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	81
PERCENT COVERAGE:	83

Validated AA Sequence

MAHHHHHHMS DEGFEDLFIS DTDPEELYEE LGPIGVGNYG SVIKARNRHT QEITAIKLVL LVDKDERDFV QREISILKRC RHPNIVHYYG AHKSLSKLWI VMEYCEGGSI DMVYKVLRHP LPEPLIAYVC RQVLLGLQYL HANRKIHRDI KGGNILLTRD GGVKLADFGV STELSHSLSR RNTFVGTLLW MAPEAILELD YDEKADLWSL GITLIEMAEG APPYIDTHPA RALFMIPKMD PPSLQDKERW SPQMTLFLKR LLTKDHNARP TATTMLNDPF VLPENIGTXE QLKAVIDEVL AXKASIDXQR XXDYXDSSAT FVERTXSDGG XGXDHXTDSX XXFXXLNSKX XXXXXXXXXX XXGXXLXXXX XXXXXXXXAX XXXXXXXXXX XXXXXXXVXX XXX

Validated NT Sequence

ttttntttaa ctttaagaag gagatatacc atggctcacc accaccacca ccatatgagc gacgaggggt ttgaagatct ctttatatcg gacaccgacc cggaggagtt gtacgaagag ttgggcccca ttggtgtagg gaactatgga tctgttataa aggcacggaa ccgtcatacg caggaaatca ctgccatcaa gctcgtcttg ttggttgaca aagatgagcg ggacttcgtg cagcgggaga ttagcatatt aaagcgttgt aggcacccga acattgtgca ctactatggt gctcacaagt ctcttagtaa gttatggatt gtaatggagt actgtgaagg tggatccatt gatatggttt ataaggtgtt gcggcacccc ttgccagaac cactgattgc ctatgtttgc cgtcaagtgt tgcttgggct gcaatacctc cacgcaaatc gaaagataca ccgagacatc aagggtggta acattttgct gacgcgtgat ggtggagtga aactcgctga ttttggtgta agcacagagc tgtcccactc cctctcgcgt cgtaacacat ttgtgggcac gttattgtgg atggcgccgg aggccatttt ggagctcgat tatgacgaga aagctgatct gtggagttta ggtattacat tgattgaaat ggcggagggc gcaccaccgt atattgacac tcacccagcg cgtgccttgt ttatgattcc gaagatggat ccaccctcgc tccaggacaa ggaacggtgg tcgccacaaa tgacactttt tctcaaaagg ttactgacaa aggatcacaa cgctcgcccg acagccacga caatgcttaa cgaccccttt gtactaccgg agaatattgg tacgcnngag cagcttaaag ccgttataga tgaggtgctt gctaanaaag cttcnataga ccngcaacgt angcncgact acncggattc nagcgcaaca ttcgtggaaa ggacnncntc tgatggngga gnnggggntg atcacngcac ggacagcnnn nngangttca nnncacttaa cagcaaangg nnnnnnannn tcncnnnann nntnnangnn ttngnngggn nngnnctcnn nnnnntcnnn anngnnnnnn cnnnnnnnnn cnnngcnnnn anntnnnann nannttnngn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggttcnnnnn nnnnnnnnnn g

Expected Protein Sequence

MAHHHHHHMS DEGFEDLFIL DTDPEELYEE LGPIGVGNYG SVIKARNRHT QEITAIKLVL LVDKDERDFV QREISILKHC RHPNIVHYYG AHKSLSKLWI VMEYCEGGSI DMVYKVLRHP LPEPLIAYVC RQVLLGLQYL HANRKIHRDI KGGNILLTRD GGVKLADFGV STELSHSLSR RNTFVGTLLW MAPEAILELD YDEKADLWSL GITLIEMAEG APPYIDTHPA RALFMIPKMD PPSLQDKERW SPQMTLFLKR LLTKDHNARP TATTMLNDPF VLPENIGTQE QLKAVIDEVL AKKASIDPQR MRDYTDSSAT FVERTSSDGG GGDDHSTDSR DEVQPHLTAK GLTNAPANSI EALDGAGTHL FFQDGTLLPL PMLYTQDISF DELCVGEELN GPFSLPVPSV ESLLVPPEQQ STACHGGEAL AHTMHVTAKL KEMLYYHKQL PYCRGLTDAE AAKSREMQLK YGSVLKSVYR L

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg acgaggggtt tgaagatctc tttatattgg acaccgaccc ggaggagttg tacgaagagt tgggccccat tggtgtaggg aactatggat ctgttataaa ggcacggaac cgtcatacgc aggaaatcac tgccatcaag ctcgtgttgt tggttgacaa agatgagcgg gacttcgtgc agcgggagat tagcatatta aagcattgta ggcacccgaa cattgtgcac tactatggtg ctcacaagtc tcttagtaag ttatggattg taatggagta ctgtgaaggt ggatccattg atatggttta taaggtgttg cggcacccct tgccagagcc actgattgcc tatgtttgcc gtcaagtgtt gcttgggctg caatacctcc acgcaaatcg aaagatacac cgagacatca agggtggtaa cattttgctg acgcgtgatg gtggagtgaa actcgctgat tttggtgtaa gcacggagtt gtcccactcc ctctcgcgtc gtaacacatt tgtgggcacg ttattgtgga tggcgccgga ggccattttg gagctcgatt atgacgagaa agctgatctg tggagtttag gtattacatt gattgaaatg gcggagggcg caccaccgta tattgacact cacccagcgc gtgccttgtt tatgattccg aagatggatc caccctcgct ccaggacaag gaacggtggt cgccacaaat gacacttttt ctcaaaaggt tactgacaaa ggatcacaac gctcgcccga cagccacgac aatgcttaac gacccctttg tactaccgga gaatattggt acgcaggagc agcttaaagc cgttatagat gaggtgcttg ctaagaaagc ttcaatagac ccgcaacgta tgcgcgacta cacggattcg agcgcaacat tcgtggaaag gacgtcttcc gatggtggag gtggggatga tcacagcacg gacagcaggg atgaggttca gccgcactta acagcaaagg gcctcaccaa cgctcccgcg aattcgatag aggcgttgga cggggccgga actcacctct tcttccagga tggtacactc ctaccactgc ctatgctcta cacacaggac ataagtttcg atgaactttg tgttggtgag gagctcaatg ggccattttc attaccagtg cctagtgtgg agtcgttgtt ggttccccct gaacagcagt caacggcttg tcacggcggc gaggcgctgg cgcacaccat gcacgtaacg gcgaagctga aggagatgct gtattaccac aagcagcttc cttactgtcg cggtctcaca gacgcggagg cagcgaagtc cagggaaatg cagctaaagt atgggtctgt gttgaagagt gtctaccgcc tgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat