SSGCID: Target Page

TrbrB.01480.b

Protein kinase, putative (EC 2.7.1.-)

CENTER ID:	TrbrB.01480.b
ORGANISM:	Trypanosoma brucei GUTat10.1
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrB.01480.b.B1.GE36995	full length		1	351

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrB.01480.b.B1.PS01866	full length		1	351

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tb927.5.4430
RefSeq:	XP_845113.1
UniProt:	Q57Z06

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSHVPFRMER LMDRLYATDE ILDFVGRGTY ASVFKGVSLV DGDKVSKGSP VALKVFEKRG LSLDRMCQDI VREVEILRHL NHPNCLQFID CFQTEYHIVI VTNFVDGKEL YKTLRDGIMT EAQVRSVARQ LLEVVNYLHS QVRVVHRDIK PENVLVTSTE DGGFHLTLVD FGLARTLSKG RRCVGDRLLG GNVWQLSPAP SAAPADSLES SYTDSPLIAT PCGTINYAAP ETVRSLVQSG HLATTAELMP RMDMFAVGTI LYVMFCRKLP FQNRGNKVQL VKVMEVGPLF EEPEWRNVPP EAVDIIRLLL SFDPGQRPKA SDVLQSAWFL KKNESGGVAD FLPMTSQCEE G

NT Sequence

atgtcgcacg ttccctttcg catggaacgt cttatggacc gcctgtatgc gacggacgaa attttagact ttgtggggcg aggaacgtat gcgtcggtgt tcaagggtgt gagcctcgta gatggtgata aagtgagcaa ggggagccct gttgccctca aggtgtttga aaagcgaggt ctctcgttgg acagaatgtg ccaagatatt gttagagagg tggagattct gcgtcatctg aaccacccta attgcttgca atttattgat tgctttcaaa ccgaatatca tattgtgatc gtgacaaact tcgttgatgg aaaggaactc tacaagacac tgcgtgacgg catcatgacg gaggcgcagg tgcggtcagt tgcacgacag ttgttggagg tcgttaacta tttgcatagc caggttcgcg ttgtacaccg cgatatcaaa cctgagaacg tacttgtaac atccacagaa gatggtggat tccacttgac tcttgttgac tttggtctcg cacgcacgct gtcgaagggg cggcgttgtg ttggtgatcg tcttttgggc gggaacgtgt ggcagttgtc cccagctccc tcggctgcgc cagcggactc gcttgagtcg tcgtacaccg acagtcctct tatcgccaca ccgtgcggga ccattaacta tgcggccccc gagacggtgc gcagcctcgt gcaaagcggc catctggcaa ccaccgctga gttaatgcca cgaatggata tgtttgccgt ggggaccata ttgtacgtga tgttctgcag gaagctgcct tttcaaaaca ggggaaataa ggtacaattg gtgaaagtga tggaggtcgg gccgttattt gaggaaccgg aatggaggaa tgtgccacct gaggctgtag atatcattcg gctattgttg agttttgacc caggtcagcg gcctaaagca agtgatgtgc tgcaatctgc gtggttccta aaaaaaaatg agtcgggtgg agtggctgat ttcttaccaa tgacgagcca atgtgaagag ggatag

Details for TrbrB.01480.b.B1.GE36995

HARVESTED ON:	5/21/2013
SEQUENCED ON:	5/23/2013
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MSHVPFRMER LMDRLYATDE ILDFVGRGTY ASVFKGVSLV DGDKVSKGSP VALKVFEKRG LSLDRMCQDI VREVEILRHL NHPNCLQFID CFQTEYHIVI VTNFVDGKEL YKTLRDGTMT EAQVRSVARQ LLEVVNYLHS QVRVVHRDIK PENVLVTSTE DGGFHLTLVD FGLARTLSKG RRCVGDRLLG GNVWQLSPAP SAAPADSLES SYTDSPLIAT PCGTINYAAP ETVRSLVQSG HLATTAELMP RMDMFAVGTI LYVMFCRKLP FQNRGNKVQL VKVMEVGPLF EEPEWRNVPP EAVDIIRLLL SFDPGQRPKA SDVLQSAWFL KKNETGGVAD FLPMTSQCEK

Validated NT Sequence

tcccttttca cattggctcg tcattggtaa gaaatcagcc actccacccg tctcattttt ttttaggaac cacgcagatt gcagcacatc acttgcttta ggccgctgac ctgggtcaaa actcaacaat agccgaatga tatctacagc ctcaggtggc acattcctcc attccggctc ctcaaataac ggcccgacct ccatcacttt caccaattgt accttatttc ccctgttttg aaaaggcagc ttcctgcaga acatcacgta caatatggtc cccacggcaa acatatccat tcgtggcatt aactcagcgg tggttgccag atggccgctt tgcacgaggc tgcgcaccgt ctcgggggcc gcatagttaa tggtcccgca cggtgtggcg ataagaggac tgtcggtgta cgacgactca agcgagtccg ctggcgcagc cgagggagct ggggacaact gccacacgtt cccgcccaaa agacgatcac caacacaacg ccgccccttc gacagcgtgc gtgcgagacc aaagtcaaca agagtcaagt ggaatccacc atcttctgtg gatgttacaa gtacgttctc aggtttgata tcgcggtgta caacgcgaac ctggctatgc aaatagttaa cgacctccaa caactgtcgt gcaactgacc gcacctgcgc ctccgtcatg gtgccgtcac gcagtgtctt gtagagttcc tttccatcaa cgaagtttgt cacgatcaca atatgatatt cggtttgaaa gcaatcaata aattgcaagc aattagggtg gttcagatga cgcagaatct ccacctctct aacaatatct tggcacattc tgtccaacga gagacctcgc ttttcaaaca ccttgagggc aacagggctc cccttgctca ctttatcacc atctacgagg ctcacaccct tgaacaccga cgcatacgtt cctcgcccca caaagtctaa aatttcgtcc gtcgcataca ggcggtccat aagacgttcc atgcgaaagg gaacgtgcga catatggtgg tggtggtggt ga

Expected Protein Sequence

MAHHHHHHMS HVPFRMERLM DRLYATDEIL DFVGRGTYAS VFKGVSLVDG DKVSKGSPVA LKVFEKRGLS LDRMCQDIVR EVEILRHLNH PNCLQFIDCF QTEYHIVIVT NFVDGKELYK TLRDGIMTEA QVRSVARQLL EVVNYLHSQV RVVHRDIKPE NVLVTSTEDG GFHLTLVDFG LARTLSKGRR CVGDRLLGGN VWQLSPAPSA APADSLESSY TDSPLIATPC GTINYAAPET VRSLVQSGHL ATTAELMPRM DMFAVGTILY VMFCRKLPFQ NRGNKVQLVK VMEVGPLFEE PEWRNVPPEA VDIIRLLLSF DPGQRPKASD VLQSAWFLKK NESGGVADFL PMTSQCEEG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcgc acgttccctt tcgcatggaa cgtcttatgg accgcctgta tgcgacggac gaaattttag actttgtggg gcgaggaacg tatgcgtcgg tgttcaaggg tgtgagcctc gtagatggtg ataaagtgag caaggggagc cctgttgccc tcaaggtgtt tgaaaagcga ggtctctcgt tggacagaat gtgccaagat attgttagag aggtggagat tctgcgtcat ctgaaccacc ctaattgctt gcaatttatt gattgctttc aaaccgaata tcatattgtg atcgtgacaa acttcgttga tggaaaggaa ctctacaaga cactgcgtga cggcatcatg acggaggcgc aggtgcggtc agttgcacga cagttgttgg aggtcgttaa ctatttgcat agccaggttc gcgttgtaca ccgcgatatc aaacctgaga acgtacttgt aacatccaca gaagatggtg gattccactt gactcttgtt gactttggtc tcgcacgcac gctgtcgaag gggcggcgtt gtgttggtga tcgtcttttg ggcgggaacg tgtggcagtt gtccccagct ccctcggctg cgccagcgga ctcgcttgag tcgtcgtaca ccgacagtcc tcttatcgcc acaccgtgcg ggaccattaa ctatgcggcc cccgagacgg tgcgcagcct cgtgcaaagc ggccatctgg caaccaccgc tgagttaatg ccacgaatgg atatgtttgc cgtggggacc atattgtacg tgatgttctg caggaagctg ccttttcaaa acaggggaaa taaggtacaa ttggtgaaag tgatggaggt cgggccgtta tttgaggaac cggaatggag gaatgtgcca cctgaggctg tagatatcat tcggctattg ttgagttttg acccaggtca gcggcctaaa gcaagtgatg tgctgcaatc tgcgtggttc ctaaaaaaaa atgagtcggg tggagtggct gatttcttac caatgacgag ccaatgtgaa gagggatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for TrbrB.01480.b.B1.PS01866

PURIFICATION DATe:	7/17/2013
CONCENTRATION:	14.53mg/ml
OBSERVED MW:	39kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCGC ACGTTCCCTT TCGCATGGAA CGTCTTATGG ACCGCCTGTA TGCGACGGAC GAAATTTTAG ACTTTGTGGG GCGAGGAACG TATGCGTCGG TGTTCAAGGG TGTGAGCCTC GTAGATGGTG ATAAAGTGAG CAAGGGGAGC CCTGTTGCCC TCAAGGTGTT TGAAAAGCGA GGTCTCTCGT TGGACAGAAT GTGCCAAGAT ATTGTTAGAG AGGTGGAGAT TCTGCGTCAT CTGAACCACC CTAATTGCTT GCAATTTATT GATTGCTTTC AAACCGAATA TCATATTGTG ATCGTGACAA ACTTCGTTGA TGGAAAGGAA CTCTACAAGA CACTGCGTGA CGGCATCATG ACGGAGGCGC AGGTGCGGTC AGTTGCACGA CAGTTGTTGG AGGTCGTTAA CTATTTGCAT AGCCAGGTTC GCGTTGTACA CCGCGATATC AAACCTGAGA ACGTACTTGT AACATCCACA GAAGATGGTG GATTCCACTT GACTCTTGTT GACTTTGGTC TCGCACGCAC GCTGTCGAAG GGGCGGCGTT GTGTTGGTGA TCGTCTTTTG GGCGGGAACG TGTGGCAGTT GTCCCCAGCT CCCTCGGCTG CGCCAGCGGA CTCGCTTGAG TCGTCGTACA CCGACAGTCC TCTTATCGCC ACACCGTGCG GGACCATTAA CTATGCGGCC CCCGAGACGG TGCGCAGCCT CGTGCAAAGC GGCCATCTGG CAACCACCGC TGAGTTAATG CCACGAATGG ATATGTTTGC CGTGGGGACC ATATTGTACG TGATGTTCTG CAGGAAGCTG CCTTTTCAAA ACAGGGGAAA TAAGGTACAA TTGGTGAAAG TGATGGAGGT CGGGCCGTTA TTTGAGGAAC CGGAATGGAG GAATGTGCCA CCTGAGGCTG TAGATATCAT TCGGCTATTG TTGAGTTTTG ACCCAGGTCA GCGGCCTAAA GCAAGTGATG TGCTGCAATC TGCGTGGTTC CTAAAAAAAA ATGAGTCGGG TGGAGTGGCT GATTTCTTAC CAATGACGAG CCAATGTGAA GAGGGATGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T