SSGCID: Target Page

TrbrB.01252.a

Protein kinase, putative (EC 2.7.1.-)

CENTER ID:	TrbrB.01252.a
ORGANISM:	Trypanosoma brucei GUTat10.1
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrB.01252.a.B1.GE36994	full length		1	342

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tb927.11.5860
RefSeq:	XP_828661.1
UniProt:	Q385I2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MCGKDSSHDY STLVTALSRT MVFKQDSHPR YEGPVALKVA AKSGSDDRGM HPSLHEAGVY KLICPHPSIP DYVDCVEDWF DTERFLAEEE RRGEQRLPVL VTQFIKAVAL DRFLCSVRVI RWSVVIHIAN QLVEVLKHIN SKGVVYRDLK LPNVIIGAGG NVWLVDYASS MIVGGEEPSS GVTSHMRPPE SFEFPDHQSG EQISQAQLSF MADFWVFGAF LLEILSGRPY LGSFDVRSSH LTQSQLEEKV LEGVSVIKDR YKGAYYNRRF NKSFPCNAKT LWDALTGLLI KLLRRNPKER LGHDGGWYAV KQQPFFKYTL CDLTVPFPLD YEDEIEELMA GF

NT Sequence

atgtgtggga aggattcatc acatgactac agtacacttg taaccgccct ttcacgaaca atggttttca agcaggactc acacccacgt tacgagggtc ctgtagcact gaaagtggca gcaaaatcgg ggtcagatga tcgaggtatg cacccgtcac tacacgaagc gggtgtctac aagctgatat gcccacatcc ttctattcct gattacgttg attgcgtgga agattggttc gacacggagc gctttctagc tgaggaggag cgccgtgggg agcagcgttt gcctgttctc gtcacacagt ttatcaaggc agttgcactt gaccgctttc tttgttctgt acgcgtaatt cggtggtccg tggtcattca catcgctaac cagctcgttg aggtacttaa gcacataaac agcaagggcg ttgtatatcg tgacctcaag ctacccaacg taataattgg agcagggggt aacgtatggc tcgtggacta cgcgagttca atgattgttg ggggtgaaga accatcaagt ggtgtaacgt ctcacatgcg tccgccagaa tcgttcgagt tcccggatca ccaaagtggg gaacaaatat cgcaagctca gctctctttc atggcggact tttgggtgtt cggcgcgttt ctactggaaa tactctctgg tcgtccatac cttgggtcct tcgacgtgcg gagtagtcat ctaacgcaga gtcaactgga ggaaaaggtg ttggaaggtg tttccgtgat taaggatcgt tacaagggtg catactacaa cagacggttt aataagagtt ttccatgcaa cgcaaagact ttatgggatg cgcttacggg gttgcttatt aaattgctgc ggcgtaatcc gaaggagagg ttaggccatg atggcggttg gtatgccgtc aagcagcaac cattcttcaa atatactctc tgtgacctca ccgtaccgtt tccattggat tatgaagatg aaattgaaga gcttatggcc ggtttttag

Details for TrbrB.01252.a.B1.GE36994

HARVESTED ON:	5/21/2013
SEQUENCED ON:	5/23/2013
EXPECTED MW:	40kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MCGKDSSHDY STLVTALSRT MVFKQDSHPR YEGPVALKVA AKSGSDDRGM HPSLHEAGVY KLICPHPSIP DYVDCVEDWF DTERFLAEEE RRGEQRLPVL VTQFIKAVAL DRFLCSVRVI RWSVVIHIAN QLVEVLKHIN SKGVVYRDLK LPNVIIGAGG SVWLVDYASS MIVGGEEPSS GVTSHMRPPE SFEFPDHQSG EQISQAQLSF MADFWVFGAF LLEILSGRPY LGSFDVRSSH LTQSQLEEKV LEGVSVIKDR YKGAYYNRRF NKSFPCNAKT LWDALTGLLI KLLRRNPKER LGHDGGWYAV KQQPFFKYTL CDLTVPFPLD YEDEIEELMA G

Validated NT Sequence

aaaaccggcc ataagctctt caatttcatc ttcataatcc aatggaaacg gtacggtgag gtcacagaga gtatatttga agaatggttg ctgcttgacg gcataccaac cgccatcatg gcctaacctc tccttcggat tacgccgcag caatttaata agcaaccccg taagcgcatc ccataaagtc tttgcgttgc atggaaaact cttattaaac cgtctgttgt agtatgcacc cttgtaacga tccttaatca cggaaacacc ttccaacacc ttttcctcca gttgactctg cgttagatga ctactccgca cgtcgaagga cccaaggtat ggacgaccag agagtatttc cagtagaaac gcgccgaaca cccaaaagtc cgccatgaaa gagagctgag cttgcgatat ttgttcccca ctttggtgat ccgggaactc gaacgattct ggcggacgca tgtgagacgt tacaccactt gatggttctt cacccccaac aatcattgaa ctcgcgtagt ccacgagcca tacgctaccc cctgctccaa ttattacgtt gggtagcttg aggtcacgat atacaacgcc cttgctgttt atgtgcttaa gtacctcaac gagctggtta gcgatgtgaa tgaccacgga ccaccgaatt acgcgtacag aacaaagaaa gcggtcaagt gcaactgcct tgataaactg tgtgacgaga acaggcaaac gctgctcccc acggcgctcc tcctcagcta gaaagcgctc cgtgtcgaac caatcttcca cgcaatcaac gtaatcagga atagaaggat gtgggcatat cagcttgtag acacccgctt cgtgtagtga cgggtgcata cctcgatcat ctgaccccga ttttgctgcc actttcagtg ctacaggacc ctcgtaacgt gggtgtgagt cctgcttgaa aaccattgtt cgtgaaaggg cggttacaag tgtactgtag tcatgtgatg aatccttccc acacatatgg tggtggtggt g

Expected Protein Sequence

MAHHHHHHMC GKDSSHDYST LVTALSRTMV FKQDSHPRYE GPVALKVAAK SGSDDRGMHP SLHEAGVYKL ICPHPSIPDY VDCVEDWFDT ERFLAEEERR GEQRLPVLVT QFIKAVALDR FLCSVRVIRW SVVIHIANQL VEVLKHINSK GVVYRDLKLP NVIIGAGGNV WLVDYASSMI VGGEEPSSGV TSHMRPPESF EFPDHQSGEQ ISQAQLSFMA DFWVFGAFLL EILSGRPYLG SFDVRSSHLT QSQLEEKVLE GVSVIKDRYK GAYYNRRFNK SFPCNAKTLW DALTGLLIKL LRRNPKERLG HDGGWYAVKQ QPFFKYTLCD LTVPFPLDYE DEIEELMAGF

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtgtg ggaaggattc atcacatgac tacagtacac ttgtaaccgc cctttcacga acaatggttt tcaagcagga ctcacaccca cgttacgagg gtcctgtagc actgaaagtg gcagcaaaat cggggtcaga tgatcgaggt atgcacccgt cactacacga agcgggtgtc tacaagctga tatgcccaca tccttctatt cctgattacg ttgattgcgt ggaagattgg ttcgacacgg agcgctttct agctgaggag gagcgccgtg gggagcagcg tttgcctgtt ctcgtcacac agtttatcaa ggcagttgca cttgaccgct ttctttgttc tgtacgcgta attcggtggt ccgtggtcat tcacatcgct aaccagctcg ttgaggtact taagcacata aacagcaagg gcgttgtata tcgtgacctc aagctaccca acgtaataat tggagcaggg ggtaacgtat ggctcgtgga ctacgcgagt tcaatgattg ttgggggtga agaaccatca agtggtgtaa cgtctcacat gcgtccgcca gaatcgttcg agttcccgga tcaccaaagt ggggaacaaa tatcgcaagc tcagctctct ttcatggcgg acttttgggt gttcggcgcg tttctactgg aaatactctc tggtcgtcca taccttgggt ccttcgacgt gcggagtagt catctaacgc agagtcaact ggaggaaaag gtgttggaag gtgtttccgt gattaaggat cgttacaagg gtgcatacta caacagacgg tttaataaga gttttccatg caacgcaaag actttatggg atgcgcttac ggggttgctt attaaattgc tgcggcgtaa tccgaaggag aggttaggcc atgatggcgg ttggtatgcc gtcaagcagc aaccattctt caaatatact ctctgtgacc tcaccgtacc gtttccattg gattatgaag atgaaattga agagcttatg gccggttttt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat