SSGCID: Target Page

TrbrB.01010.d

Mitogen-activated protein kinase, putative (EC 2.7.1.-)

CENTER ID:	TrbrB.01010.d
ORGANISM:	Trypanosoma brucei GUTat10.1
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrB.01010.d.B1.GE36993	full length		1	409

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrB.01010.d.B1.PW37371	full length		1	409

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tb927.8.3770
RefSeq:	XP_847208.1
UniProt:	Q580Z7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGSKGEELMQ ELIVELREMN SRYTVHRFLS SGSYGAVFAG VDESDLPVAI KRVFNTISEG RTINILSDPF LCKRVLREIM LLNHFHHPNI LGLKNMFIHL QPPGQHKLYL VTDLMRTDLA QVIHDKRIDI SPQHIRFFMY HIILGLHVLH EAGVVHRDLH PGNVLVSENN DITICDFNLA REDTQDPNKT HYVTHRWYRA PELVMQFKGF TKLVDMWSAG CVMGELFNRR ALFRGSTFYN QLDKIVEVVG APTEEDHLDM FSSSQARNYL RNALSHCKPR PWCDVVPTAD AEAIDLLSRM LEFNPARRIS AEQALQHPYF ASLYDPLDLT EACSGPFHFP EVSDIFSMHQ MFLAEVRGFE ARRLKRENII QQRLALSNGT TNAKEVLGCD HMLRTHSLME VSDLPTSMP

NT Sequence

atgggttcta aaggcgagga gctgatgcaa gagctcattg tggaattacg ggagatgaat tctcgttata cggtgcaccg ttttctcagc agtgggagct atggggctgt ttttgcaggc gtggatgaaa gcgatcttcc cgtggcgatt aaacgggtgt tcaacaccat ctctgagggt cggaccataa atatcctctc cgatcctttt ctctgcaaac gggtgttgcg cgagattatg ctgctgaacc acttccacca cccaaacata ctcggactga agaacatgtt catacatctg cagcccccgg gccagcacaa actgtacctc gtcaccgacc tcatgcgtac cgacctggca caagttatac acgataagcg cattgatatt tctccccagc acatccgctt cttcatgtac cacatcatac ttggattgca tgttctgcac gaagcgggtg ttgtacatcg tgatctgcac ccaggaaatg tacttgtctc tgagaacaat gacatcacca tatgtgactt caatctggca cgagaggata cgcaggatcc aaataaaaca cactacgtga cgcaccgctg gtaccgcgca ccggaactcg tgatgcagtt caaaggattc acgaaacttg tcgatatgtg gtctgctggg tgcgtaatgg gtgagctttt caaccgtcgt gccctttttc gtggttctac attctacaac cagttagaca aaattgtgga agttgtagga gcgcccacgg aggaagacca ccttgatatg ttctcatctt cgcaggcgcg gaattacctt cgtaacgccc tctcgcactg caagccccgg ccatggtgtg atgtggtgcc cactgccgat gctgaagcaa tagatttgct cagtcgtatg cttgaattca atccagcgcg gcgtatcagt gctgagcagg ctttgcagca tccctatttt gcatctctat atgacccact ggatcttacg gaggcatgca gtggtccttt ccatttccca gaggtgagcg acatcttctc catgcatcaa atgttcttgg cggaggttcg tggctttgag gcacggcggt taaagcgaga gaatatcata caacaacgac tcgcactttc aaatggcacc acaaatgcga aggaagtact cggctgtgac catatgctgc ggactcatag tcttatggaa gtgtcagatc ttcccacctc tatgccctaa

Details for TrbrB.01010.d.B1.GE36993

HARVESTED ON:	5/21/2013
SEQUENCED ON:	5/23/2013
EXPECTED MW:	48kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

MQELIVELRE MNSRYTVHRF LSSGSYGAVF AGVDESDLPV AIKRVFNTIS EGRTINILSD PFLCKRVLRE IMLLNHFHHP NILGLKNMFI HLQPPGQHKL YLVTDLMRTD LAQVIHDKRI DISPQHIRFF MYHIILGLHV LHEAGVVHRD LHPGNVLVSE NNDITICDFN LAREDTQDPN KTHYVTHRWY RAPELVMQFK GFTKLVDMWS AGCVMGELFN RRALFRGSTF YNQLDKIVEV VGAPTEEDHL DMFSSSQARN YLRNALSHCK PRPWCDVVPT ADAEAIDLLS RMLEFNPARR ISAEQALQHP YFASLYDPLD LTEACSGPFH FPEVSDIFSM HQMFLAEVRG FEARRLKREN IIQQRLALSN GTTNAKEVLG CDHMLRTHSL M

Validated NT Sequence

tccataagac tatgagtccg cagcatatgg tcacagccga gtacttcctt cgcatttgtg gtgccattcg aaagtgcgag tcgttgttgt atgatattct ctctctttaa ccgccgagcc tcaaagccac gaacctccgc caagaacatt tgatgcatgg agaagatgtc gctcacctct gggaaatgga aaggaccact gcatgcctcc gtaagatcca gtgggtcata tagagatgca aaatagggat gctgcaaagc ctgctcagca ctgatacgcc gcgctggatt gaattcaagc atacgactga gcaaatctat tgcttcagca tcggcagtgg gcaccacatc acaccatggc cggggcttgc agtgcgagag ggcgttacga aggtaattcc gcgcctgcga agatgagaac atatcaaggt ggtcttcctc cgtgggcgct cctacaactt ccacaatttt gtctaactgg ttgtagaatg tagaaccacg aaaaagggca cgacggttga aaagctcacc cattacgcac ccagcagacc acatatcgac aagtttcgtg aatcctttga actgcatcac gagttccggt gcgcggtacc agcggtgcgt cacgtagtgt gttttatttg gatcctgcgt atcctctcgt gccagattga agtcacatat ggtgatgtca ttgttctcag agacaagtac atttcctggg tgcagatcac gatgtacaac acccgcttcg tgcagaacat gcaatccaag tatgatgtgg tacatgaaga agcggatgtg ctggggagaa atatcaatgc gcttatcgtg tataacttgt gccaggtcgg tacgcatgag gtcggtgacg aggtacagtt tgtgctggcc cgggggctgc agatgtatga acatgttctt cagtccgagt atgtttgggt ggtggaagtg gttcagcagc ataatctccc gcaacacccg tttgcagaga aaaggatcgg agaggatatt tatggtccga ccctcagaga tggtgttgaa cacccgttta atcgccacgg gaagatcgct ttcatccacg cctgcaaaaa cagccccata gctcccactg ctgagaaaac ggtgcaccgt ataacgagaa ttcatctccc gtaattccac aatgagctct tgcatcagct cctcgcctt

Expected Protein Sequence

MAHHHHHHMG SKGEELMQEL IVELREMNSR YTVHRFLSSG SYGAVFAGVD ESDLPVAIKR VFNTISEGRT INILSDPFLC KRVLREIMLL NHFHHPNILG LKNMFIHLQP PGQHKLYLVT DLMRTDLAQV IHDKRIDISP QHIRFFMYHI ILGLHVLHEA GVVHRDLHPG NVLVSENNDI TICDFNLARE DTQDPNKTHY VTHRWYRAPE LVMQFKGFTK LVDMWSAGCV MGELFNRRAL FRGSTFYNQL DKIVEVVGAP TEEDHLDMFS SSQARNYLRN ALSHCKPRPW CDVVPTADAE AIDLLSRMLE FNPARRISAE QALQHPYFAS LYDPLDLTEA CSGPFHFPEV SDIFSMHQMF LAEVRGFEAR RLKRENIIQQ RLALSNGTTN AKEVLGCDHM LRTHSLMEVS DLPTSMP

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggtt ctaaaggcga ggagctgatg caagagctca ttgtggaatt acgggagatg aattctcgtt atacggtgca ccgttttctc agcagtggga gctatggggc tgtttttgca ggcgtggatg aaagcgatct tcccgtggcg attaaacggg tgttcaacac catctctgag ggtcggacca taaatatcct ctccgatcct tttctctgca aacgggtgtt gcgcgagatt atgctgctga accacttcca ccacccaaac atactcggac tgaagaacat gttcatacat ctgcagcccc cgggccagca caaactgtac ctcgtcaccg acctcatgcg taccgacctg gcacaagtta tacacgataa gcgcattgat atttctcccc agcacatccg cttcttcatg taccacatca tacttggatt gcatgttctg cacgaagcgg gtgttgtaca tcgtgatctg cacccaggaa atgtacttgt ctctgagaac aatgacatca ccatatgtga cttcaatctg gcacgagagg atacgcagga tccaaataaa acacactacg tgacgcaccg ctggtaccgc gcaccggaac tcgtgatgca gttcaaagga ttcacgaaac ttgtcgatat gtggtctgct gggtgcgtaa tgggtgagct tttcaaccgt cgtgcccttt ttcgtggttc tacattctac aaccagttag acaaaattgt ggaagttgta ggagcgccca cggaggaaga ccaccttgat atgttctcat cttcgcaggc gcggaattac cttcgtaacg ccctctcgca ctgcaagccc cggccatggt gtgatgtggt gcccactgcc gatgctgaag caatagattt gctcagtcgt atgcttgaat tcaatccagc gcggcgtatc agtgctgagc aggctttgca gcatccctat tttgcatctc tatatgaccc actggatctt acggaggcat gcagtggtcc tttccatttc ccagaggtga gcgacatctt ctccatgcat caaatgttct tggcggaggt tcgtggcttt gaggcacggc ggttaaagcg agagaatatc atacaacaac gactcgcact ttcaaatggc accacaaatg cgaaggaagt actcggctgt gaccatatgc tgcggactca tagtcttatg gaagtgtcag atcttcccac ctctatgccc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for TrbrB.01010.d.B1.PW37371

PURIFICATION DATe:	10/1/2013
CONCENTRATION:	42.2mg/ml
OBSERVED MW:	43kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGTT CTAAAGGCGA GGAGCTGATG CAAGAGCTCA TTGTGGAATT ACGGGAGATG AATTCTCGTT ATACGGTGCA CCGTTTTCTC AGCAGTGGGA GCTATGGGGC TGTTTTTGCA GGCGTGGATG AAAGCGATCT TCCCGTGGCG ATTAAACGGG TGTTCAACAC CATCTCTGAG GGTCGGACCA TAAATATCCT CTCCGATCCT TTTCTCTGCA AACGGGTGTT GCGCGAGATT ATGCTGCTGA ACCACTTCCA CCACCCAAAC ATACTCGGAC TGAAGAACAT GTTCATACAT CTGCAGCCCC CGGGCCAGCA CAAACTGTAC CTCGTCACCG ACCTCATGCG TACCGACCTG GCACAAGTTA TACACGATAA GCGCATTGAT ATTTCTCCCC AGCACATCCG CTTCTTCATG TACCACATCA TACTTGGATT GCATGTTCTG CACGAAGCGG GTGTTGTACA TCGTGATCTG CACCCAGGAA ATGTACTTGT CTCTGAGAAC AATGACATCA CCATATGTGA CTTCAATCTG GCACGAGAGG ATACGCAGGA TCCAAATAAA ACACACTACG TGACGCACCG CTGGTACCGC GCACCGGAAC TCGTGATGCA GTTCAAAGGA TTCACGAAAC TTGTCGATAT GTGGTCTGCT GGGTGCGTAA TGGGTGAGCT TTTCAACCGT CGTGCCCTTT TTCGTGGTTC TACATTCTAC AACCAGTTAG ACAAAATTGT GGAAGTTGTA GGAGCGCCCA CGGAGGAAGA CCACCTTGAT ATGTTCTCAT CTTCGCAGGC GCGGAATTAC CTTCGTAACG CCCTCTCGCA CTGCAAGCCC CGGCCATGGT GTGATGTGGT GCCCACTGCC GATGCTGAAG CAATAGATTT GCTCAGTCGT ATGCTTGAAT TCAATCCAGC GCGGCGTATC AGTGCTGAGC AGGCTTTGCA GCATCCCTAT TTTGCATCTC TATATGACCC ACTGGATCTT ACGGAGGCAT GCAGTGGTCC TTTCCATTTC CCAGAGGTGA GCGACATCTT CTCCATGCAT CAAATGTTCT TGGCGGAGGT TCGTGGCTTT GAGGCACGGC GGTTAAAGCG AGAGAATATC ATACAACAAC GACTCGCACT TTCAAATGGC ACCACAAATG CGAAGGAAGT ACTCGGCTGT GACCATATGC TGCGGACTCA TAGTCTTATG GAAGTGTCAG ATCTTCCCAC CTCTATGCCC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT