TrbrB.01010.b

Casein kinase II, alpha chain (EC 2.7.1.37) (EC 2.7.11.1)

CENTER ID: TrbrB.01010.b
ORGANISM: Trypanosoma brucei GUTat10.1
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrB.01010.b.B1.GE36992 full length 1 372
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tb927.2.2430
RefSeq: XP_951545.1
UniProt: Q586L5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRSGGRAAT EKGLGNANSS QLAETSGQSG YAHPFWYVNT QMPPEYWDYE NATLEFNSDE PYELLQKIGR GKYSEVFRAR SRHNGELCVL KILKPVRVKK IRREITILQN LCGGPNVVRL LDVVMVEQEN TPVLVTENIE PADNFRTLMN SGSLTSFDMR YYMYEILRCL HFAHSRGIFH RDIKPQNIII DHRRRKLRIV DWGLAEYYIH GQAYNLGVGT RHFKGPELLV GIRLYDYSLD MWSVGCVLAE LLFRVFPLFR GEDNEDQLHQ IASVVGTEDI MRYVQKYDVS LPRHLVDRAL STKYPKRPWK SFVTTRNIDW CDANGLDLLD KMLQVDHQER ILAYDAMQHP FFDPVRKVLQ GDPQEQYPRR RG
NT Sequence
atgtcccgca gcggtggtcg cgcggcgact gaaaagggtt tagggaatgc taatagcagt caactggccg agacaagtgg ccaaagcgga tacgcccatc ccttttggta cgttaatacg cagatgccac cggagtattg ggactacgag aatgccacac ttgagttcaa cagtgatgag ccgtacgagc ttctgcagaa aattggtcgt ggaaaatact cggaggtgtt tcgggcgcgc agccggcaca acggggaact ttgtgtgctg aagatcctta aaccagtgag ggtaaagaaa ataagaaggg aaatcactat cctgcaaaac ctctgcgggg gaccaaacgt cgtgaggctg cttgacgttg tcatggttga acaggagaat acgcctgtgc ttgtcacaga gaacatagaa ccagctgata acttccgtac tctcatgaac agcggtagtc tcacatcttt tgacatgcgc tattacatgt atgaaatact gcgctgtctg catttcgctc acagtcgagg aatatttcat cgcgacatca aaccgcagaa catcatcatt gaccaccgcc gccgcaagct tcgtatcgta gactggggcc ttgcagagta ttacattcat ggccaagcgt ataacttagg tgtagggact cgacatttta aaggtcctga gcttcttgtg ggaatacggc tttatgatta ctctttggac atgtggagtg ttggttgtgt ccttgccgag ttactcttcc gggtgtttcc tcttttccgt ggagaagata atgaggacca actgcatcag atagcaagcg tagtcggcac agaagatata atgcgctacg ttcaaaaata tgatgtttca ctccctcgac acctggttga cagggccctt tccacaaaat atccaaagag gccgtggaaa tcattcgtca ccacccgaaa catagattgg tgcgatgcaa atgggttgga cctgcttgac aaaatgctgc aggtcgatca tcaggaacga attcttgcct acgatgcaat gcagcacccg ttttttgatc cagtacgcaa ggtgttgcag ggagacccac aggaacagta cccgaggcgc agaggttag
Details for TrbrB.01010.b.B1.GE36992
HARVESTED ON: 5/21/2013
SEQUENCED ON: 5/23/2013
EXPECTED MW: 44kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 88
Validated AA Sequence
MAHHHHHHMS RSGGRATTEK GLGNANSSQL AETSGQSGYA HPFWYVNTQM PPEYWDYENA TLEFNSDEPY ELLQKIGRGK YSEVFRARSR HNGELCVLKI LKPVRVKKIR REITILQNLC GGPNVVRLLD VVMVEQENTP VLVTENIEPA DNFRTLMNSG SLTSFDMRYY MYEILRCLHF AHSRGIFHRD IKPQNIIIDH RRRKLRIVDW GLAEYYIHGQ AYNLGVGTRH FKGPELLVGI RLYDYSLDMW SVGCVLAELL FRVFPLFRGE DNEDQLHQIA SVVGTEXIMR YVQKYDVSLP RHLVDRXLSX KYPKRPWXSF VTTXNIXXXC KWVXXA
Validated NT Sequence
attttgttta actttaagaa ggagatatac catggctcac caccaccacc accatatgtc ccgcagcggt ggtcgcgcga cgactgaaaa gggtttaggg aatgctaata gcagtcaact ggccgagaca agtggccaaa gcggatacgc ccatcccttt tggtacgtta atacgcagat gccaccggag tattgggact acgagaatgc cacacttgag ttcaacagtg atgagccgta cgagcttctg cagaaaattg gtcgtggaaa atactcggag gtgtttcggg cgcgcagccg gcacaacggg gaactttgtg tgctgaagat ccttaaacca gtgagggtaa agaaaataag aagggaaatc actatcctgc aaaacctctg cgggggacca aacgtcgtga ggctgcttga cgttgtcatg gttgaacagg agaatacgcc tgtgcttgtc acagagaaca tagaaccagc tgataacttc cgtactctca tgaacagcgg tagtctcaca tcttttgaca tgcgctatta catgtatgaa atactgcgct gtctgcattt cgctcacagt cgaggaatat ttcatcgcga catcaaaccg cagaacatca tcattgatca ccgccgccgc aagcttcgta tcgtagactg gggccttgca gagtattaca ttcatggcca agcgtataac ttaggtgtag ggactcgaca ttttaaaggt cctgagcttc ttgtgggaat acggctttat gattactctt tggacatgtg gagtgttggt tgtgtccttg ccgagttact cttccgggtg tttcctcttt tccgtggaga agataatgag gaccaactgc atcagatagc aagcgtagtc ggcacagaag anataatgcg ctacgttcaa aaatatgatg tttcactccc tcggcacctg gttgacaggn ncctttccnc aaaatatcca aagaggccgt ggaantcatt cgtcaccacc ngaaacatan angnncnatg caaatgggtt nganctgctt gacnaaatgc tgcngtcgat catcnganna ntnnntgcna cnatgcantg cagcacccnt tttnnnncng tannnnngnn ntnnnggnna cnnnnnnnnn nnnnnngnnn nntnnnnnnn nnnnnnngna nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnt
Expected Protein Sequence
MAHHHHHHMS RSGGRAATEK GLGNANSSQL AETSGQSGYA HPFWYVNTQM PPEYWDYENA TLEFNSDEPY ELLQKIGRGK YSEVFRARSR HNGELCVLKI LKPVRVKKIR REITILQNLC GGPNVVRLLD VVMVEQENTP VLVTENIEPA DNFRTLMNSG SLTSFDMRYY MYEILRCLHF AHSRGIFHRD IKPQNIIIDH RRRKLRIVDW GLAEYYIHGQ AYNLGVGTRH FKGPELLVGI RLYDYSLDMW SVGCVLAELL FRVFPLFRGE DNEDQLHQIA SVVGTEDIMR YVQKYDVSLP RHLVDRALST KYPKRPWKSF VTTRNIDWCD ANGLDLLDKM LQVDHQERIL AYDAMQHPFF DPVRKVLQGD PQEQYPRRRG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccc gcagcggtgg tcgcgcggcg actgaaaagg gtttagggaa tgctaatagc agtcaactgg ccgagacaag tggccaaagc ggatacgccc atcccttttg gtacgttaat acgcagatgc caccggagta ttgggactac gagaatgcca cacttgagtt caacagtgat gagccgtacg agcttctgca gaaaattggt cgtggaaaat actcggaggt gtttcgggcg cgcagccggc acaacgggga actttgtgtg ctgaagatcc ttaaaccagt gagggtaaag aaaataagaa gggaaatcac tatcctgcaa aacctctgcg ggggaccaaa cgtcgtgagg ctgcttgacg ttgtcatggt tgaacaggag aatacgcctg tgcttgtcac agagaacata gaaccagctg ataacttccg tactctcatg aacagcggta gtctcacatc ttttgacatg cgctattaca tgtatgaaat actgcgctgt ctgcatttcg ctcacagtcg aggaatattt catcgcgaca tcaaaccgca gaacatcatc attgaccacc gccgccgcaa gcttcgtatc gtagactggg gccttgcaga gtattacatt catggccaag cgtataactt aggtgtaggg actcgacatt ttaaaggtcc tgagcttctt gtgggaatac ggctttatga ttactctttg gacatgtgga gtgttggttg tgtccttgcc gagttactct tccgggtgtt tcctcttttc cgtggagaag ataatgagga ccaactgcat cagatagcaa gcgtagtcgg cacagaagat ataatgcgct acgttcaaaa atatgatgtt tcactccctc gacacctggt tgacagggcc ctttccacaa aatatccaaa gaggccgtgg aaatcattcg tcaccacccg aaacatagat tggtgcgatg caaatgggtt ggacctgctt gacaaaatgc tgcaggtcga tcatcaggaa cgaattcttg cctacgatgc aatgcagcac ccgttttttg atccagtacg caaggtgttg cagggagacc cacaggaaca gtacccgagg cgcagaggtt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat