TrbrB.01010.a

Mitogen-activated protein kinase 3, putative (EC 2.7.1.-)

CENTER ID: TrbrB.01010.a
ORGANISM: Trypanosoma brucei GUTat10.1
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrB.01010.a.B1.GE36991 full length 1 371
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrB.01010.a.B1.PS01872 full length 1 371

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tb927.8.3550
RefSeq: XP_847186.1
UniProt: Q580X5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHKSNQELCE PTIVGGFKVY NVGGSEFEVP RKYTLLKILG MGAYGIACSC LNEETQEKVS IKKCREVFRD LDDGRRVLRE VAMMRFFRHE NLLHVMDILP PMKGYNEFRD VYIVTPLKDV DMNVVLRSRQ VLEETHVRYF IYQILRGLKY LHSAGVAHRD LKPANLVTDI SCELKIIDFG LSRSVTIPHH DLTDYVITRW YRPPELLLEN SYYDTAVDIW SVGCIMAEMY NRKPVLPGRN TIDQLRLICT HIGKPPRDMV ESAEALEKLN QLPDGELDMG KLVPGLTSAD GIDFLSQMWE LDPRKRPSAA ELLRHPFMAP LHDEVDEPVC PTSFAWPYEM QEMSLDSLRR AFWDEICSFN PHLASQIPKG A
NT Sequence
atgcacaaaa gtaatcaaga gttgtgcgag cctacaattg tagggggctt taaggtgtac aatgttggtg gatccgagtt tgaggttcca cggaagtaca cacttttgaa aatacttggt atgggagctt atggtattgc gtgcagttgc ttgaatgaag aaacacagga gaaggtatcg ataaaaaagt gccgagaggt attccgtgac ttggatgatg gcaggcgcgt tctgcgtgag gttgcgatga tgcgcttctt caggcacgag aatttgttac atgtcatgga tatattgccg cccatgaagg gctacaatga attccgtgat gtttacatag taacgcccct gaaggatgtt gatatgaatg ttgtgcttcg ctcgcgtcag gtgctagagg aaactcacgt ccggtacttc atttatcaaa tacttcgggg tttaaaatat ttacatagtg ccggtgtggc acaccgtgac ttgaaaccag ccaacctagt cactgacatc tcgtgcgagc tgaagattat tgacttcggt ctttcccgta gcgtcactat cccacatcat gatttgactg actacgtcat tacacgctgg taccgaccgc cggaactgct tctagaaaac agttactacg acaccgcggt agacatttgg tcagtcggtt gcattatggc agaaatgtac aatcgcaagc cagtccttcc gggccgaaac acgattgatc agctgcggct catttgcaca catattggga agcctccaag ggatatggta gagagtgcag aggcgttgga gaagttaaat caacttcctg atggcgaatt ggacatgggc aaacttgtgc ccgggttgac aagtgcagat ggaattgatt ttctcagcca aatgtgggag cttgacccgc ggaaaaggcc atcagcggct gaattacttc gccatccatt catggcaccg ttgcatgatg aagtggacga gccggtgtgt ccgacatcct tcgcgtggcc atatgaaatg caggaaatga gtcttgactc cctccgtagg gcattctggg atgaaatatg cagctttaat ccacatcttg caagccaaat accgaaaggc gcgtag
Details for TrbrB.01010.a.B1.GE36991
HARVESTED ON: 5/21/2013
SEQUENCED ON: 5/23/2013
EXPECTED MW: 44kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 84
Validated AA Sequence
MGAYGIACSC LNEETQEKVS IKKCREVFRD LDDGRRVLRE VAMMRFFRHE NLLHVMDILP PMKGYNEFRD VYIVTPLKDV DMNVVLRSRQ VLEETHVRYF IYQILRGLKY LHSAGVAHRD LKPANLVTDI SCELKIIDFG LSRSVTIPHH DLTDYVITRW YRPPELLLEN SYYDTAVDIW SVGCIMAEMY NRKPVLPGRN TIDQLRLICT HIGKPPRDMV ESAEALEKLN QLPDGELDMG KLVPGLTSAD GIDFLSQMWE LDPRKRPSAA ELLRHPFMAP LHDEVDEPVC PTSFAWPYEM QEMSLDSLRR AFWDEICSFN
Validated NT Sequence
ggattaaagc tgcatatttc atcccagaat gccctacgga gggagtcaag actcatttcc tgcatttcat atggccacgc gaaggatgtt ggacacaccg gctcgtccac ttcatcatgc aacggtgcca tgaatggatg gcgaagtaat tcagccgctg atggcctttt ccgcgggtca agctcccaca tttggctgag aaaatcaatt ccatctgcac ttgtcaaccc gggcacaagt ttgcccatgt ccaattcgcc atcaggaagt tgatttaact tctccaacgc ctctgcactc tctaccatat cccttggagg cttcccaata tgtgtgcaaa tgagccgcag ctgatcaatc gtgtttcggc ccggaaggac tggcttgcga ttgtacattt ctgccataat gcaaccgact gaccaaatgt ctactgcggt gtcgtagtaa ctgttttcta gaagcagttc cggcggtcgg taccagcgtg taatgacgta gtcagtcaaa tcatgatgtg ggatagtgac gctacgggaa agaccgaagt caataatctt cagctcgcac gagatgtcag tgactaggtt ggctggtttc aagtcacggt gtgccacacc ggcactatgt aaatatttta aaccccgaag tatttgataa atgaagtacc ggacgtgagt ttcctctagc acctgacgcg agcgaagcac aacattcata tcaacatcct tcaggggcgt tactatgtaa acatcacgga attcattgta gcccttcatg ggcggcaata tatccatgac atgtaacaaa ttctcgtgcc tgaagaagcg catcatcgca acctcacgca gaacgcgcct gccatcatcc aagtcacgga atacctctcg gcactttttt atcgatacct tctcctgtgt ttcttcattc aagcaactgc acgcaatacc ataagctccc ataccaagta ttttcaaaag tgtgtacttc cgtggaacct caaactcgga tccaccaaca ttgtacacct taaagccccc tacaattgta ggctcgcaca actcttgatt ac
Expected Protein Sequence
MAHHHHHHMH KSNQELCEPT IVGGFKVYNV GGSEFEVPRK YTLLKILGMG AYGIACSCLN EETQEKVSIK KCREVFRDLD DGRRVLREVA MMRFFRHENL LHVMDILPPM KGYNEFRDVY IVTPLKDVDM NVVLRSRQVL EETHVRYFIY QILRGLKYLH SAGVAHRDLK PANLVTDISC ELKIIDFGLS RSVTIPHHDL TDYVITRWYR PPELLLENSY YDTAVDIWSV GCIMAEMYNR KPVLPGRNTI DQLRLICTHI GKPPRDMVES AEALEKLNQL PDGELDMGKL VPGLTSADGI DFLSQMWELD PRKRPSAAEL LRHPFMAPLH DEVDEPVCPT SFAWPYEMQE MSLDSLRRAF WDEICSFNPH LASQIPKGA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaca aaagtaatca agagttgtgc gagcctacaa ttgtaggggg ctttaaggtg tacaatgttg gtggatccga gtttgaggtt ccacggaagt acacactttt gaaaatactt ggtatgggag cttatggtat tgcgtgcagt tgcttgaatg aagaaacaca ggagaaggta tcgataaaaa agtgccgaga ggtattccgt gacttggatg atggcaggcg cgttctgcgt gaggttgcga tgatgcgctt cttcaggcac gagaatttgt tacatgtcat ggatatattg ccgcccatga agggctacaa tgaattccgt gatgtttaca tagtaacgcc cctgaaggat gttgatatga atgttgtgct tcgctcgcgt caggtgctag aggaaactca cgtccggtac ttcatttatc aaatacttcg gggtttaaaa tatttacata gtgccggtgt ggcacaccgt gacttgaaac cagccaacct agtcactgac atctcgtgcg agctgaagat tattgacttc ggtctttccc gtagcgtcac tatcccacat catgatttga ctgactacgt cattacacgc tggtaccgac cgccggaact gcttctagaa aacagttact acgacaccgc ggtagacatt tggtcagtcg gttgcattat ggcagaaatg tacaatcgca agccagtcct tccgggccga aacacgattg atcagctgcg gctcatttgc acacatattg ggaagcctcc aagggatatg gtagagagtg cagaggcgtt ggagaagtta aatcaacttc ctgatggcga attggacatg ggcaaacttg tgcccgggtt gacaagtgca gatggaattg attttctcag ccaaatgtgg gagcttgacc cgcggaaaag gccatcagcg gctgaattac ttcgccatcc attcatggca ccgttgcatg atgaagtgga cgagccggtg tgtccgacat ccttcgcgtg gccatatgaa atgcaggaaa tgagtcttga ctccctccgt agggcattct gggatgaaat atgcagcttt aatccacatc ttgcaagcca aataccgaaa ggcgcgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for TrbrB.01010.a.B1.PS01872
PURIFICATION DATe: 7/23/2013
CONCENTRATION: 53mg/ml
OBSERVED MW: 37kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 84
Protocol Notes
notes unavailable
Validated AA Sequence
MGAYGIACSC LNEETQEKVS IKKCREVFRD LDDGRRVLRE VAMMRFFRHE NLLHVMDILP PMKGYNEFRD VYIVTPLKDV DMNVVLRSRQ VLEETHVRYF IYQILRGLKY LHSAGVAHRD LKPANLVTDI SCELKIIDFG LSRSVTIPHH DLTDYVITRW YRPPELLLEN SYYDTAVDIW SVGCIMAEMY NRKPVLPGRN TIDQLRLICT HIGKPPRDMV ESAEALEKLN QLPDGELDMG KLVPGLTSAD GIDFLSQMWE LDPRKRPSAA ELLRHPFMAP LHDEVDEPVC PTSFAWPYEM QEMSLDSLRR AFWDEICSFN
Validated NT Sequence
ggattaaagc tgcatatttc atcccagaat gccctacgga gggagtcaag actcatttcc tgcatttcat atggccacgc gaaggatgtt ggacacaccg gctcgtccac ttcatcatgc aacggtgcca tgaatggatg gcgaagtaat tcagccgctg atggcctttt ccgcgggtca agctcccaca tttggctgag aaaatcaatt ccatctgcac ttgtcaaccc gggcacaagt ttgcccatgt ccaattcgcc atcaggaagt tgatttaact tctccaacgc ctctgcactc tctaccatat cccttggagg cttcccaata tgtgtgcaaa tgagccgcag ctgatcaatc gtgtttcggc ccggaaggac tggcttgcga ttgtacattt ctgccataat gcaaccgact gaccaaatgt ctactgcggt gtcgtagtaa ctgttttcta gaagcagttc cggcggtcgg taccagcgtg taatgacgta gtcagtcaaa tcatgatgtg ggatagtgac gctacgggaa agaccgaagt caataatctt cagctcgcac gagatgtcag tgactaggtt ggctggtttc aagtcacggt gtgccacacc ggcactatgt aaatatttta aaccccgaag tatttgataa atgaagtacc ggacgtgagt ttcctctagc acctgacgcg agcgaagcac aacattcata tcaacatcct tcaggggcgt tactatgtaa acatcacgga attcattgta gcccttcatg ggcggcaata tatccatgac atgtaacaaa ttctcgtgcc tgaagaagcg catcatcgca acctcacgca gaacgcgcct gccatcatcc aagtcacgga atacctctcg gcactttttt atcgatacct tctcctgtgt ttcttcattc aagcaactgc acgcaatacc ataagctccc ataccaagta ttttcaaaag tgtgtacttc cgtggaacct caaactcgga tccaccaaca ttgtacacct taaagccccc tacaattgta ggctcgcaca actcttgatt ac
Expressed Protein Sequence
MAHHHHHHMH KSNQELCEPT IVGGFKVYNV GGSEFEVPRK YTLLKILGMG AYGIACSCLN EETQEKVSIK KCREVFRDLD DGRRVLREVA MMRFFRHENL LHVMDILPPM KGYNEFRDVY IVTPLKDVDM NVVLRSRQVL EETHVRYFIY QILRGLKYLH SAGVAHRDLK PANLVTDISC ELKIIDFGLS RSVTIPHHDL TDYVITRWYR PPELLLENSY YDTAVDIWSV GCIMAEMYNR KPVLPGRNTI DQLRLICTHI GKPPRDMVES AEALEKLNQL PDGELDMGKL VPGLTSADGI DFLSQMWELD PRKRPSAAEL LRHPFMAPLH DEVDEPVCPT SFAWPYEMQE MSLDSLRRAF WDEICSFNPH LASQIPKGA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCACA AAAGTAATCA AGAGTTGTGC GAGCCTACAA TTGTAGGGGG CTTTAAGGTG TACAATGTTG GTGGATCCGA GTTTGAGGTT CCACGGAAGT ACACACTTTT GAAAATACTT GGTATGGGAG CTTATGGTAT TGCGTGCAGT TGCTTGAATG AAGAAACACA GGAGAAGGTA TCGATAAAAA AGTGCCGAGA GGTATTCCGT GACTTGGATG ATGGCAGGCG CGTTCTGCGT GAGGTTGCGA TGATGCGCTT CTTCAGGCAC GAGAATTTGT TACATGTCAT GGATATATTG CCGCCCATGA AGGGCTACAA TGAATTCCGT GATGTTTACA TAGTAACGCC CCTGAAGGAT GTTGATATGA ATGTTGTGCT TCGCTCGCGT CAGGTGCTAG AGGAAACTCA CGTCCGGTAC TTCATTTATC AAATACTTCG GGGTTTAAAA TATTTACATA GTGCCGGTGT GGCACACCGT GACTTGAAAC CAGCCAACCT AGTCACTGAC ATCTCGTGCG AGCTGAAGAT TATTGACTTC GGTCTTTCCC GTAGCGTCAC TATCCCACAT CATGATTTGA CTGACTACGT CATTACACGC TGGTACCGAC CGCCGGAACT GCTTCTAGAA AACAGTTACT ACGACACCGC GGTAGACATT TGGTCAGTCG GTTGCATTAT GGCAGAAATG TACAATCGCA AGCCAGTCCT TCCGGGCCGA AACACGATTG ATCAGCTGCG GCTCATTTGC ACACATATTG GGAAGCCTCC AAGGGATATG GTAGAGAGTG CAGAGGCGTT GGAGAAGTTA AATCAACTTC CTGATGGCGA ATTGGACATG GGCAAACTTG TGCCCGGGTT GACAAGTGCA GATGGAATTG ATTTTCTCAG CCAAATGTGG GAGCTTGACC CGCGGAAAAG GCCATCAGCG GCTGAATTAC TTCGCCATCC ATTCATGGCA CCGTTGCATG ATGAAGTGGA CGAGCCGGTG TGTCCGACAT CCTTCGCGTG GCCATATGAA ATGCAGGAAA TGAGTCTTGA CTCCCTCCGT AGGGCATTCT GGGATGAAAT ATGCAGCTTT AATCCACATC TTGCAAGCCA AATACCGAAA GGCGCGTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T