SSGCID: Target Page

TrbrB.01003.b

Mitogen-activated protein kinase, putative

CENTER ID:	TrbrB.01003.b
ORGANISM:	Trypanosoma brucei GUTat10.1
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrB.01003.b.B1.GE36990	full length		1	365

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tb927.10.16030
RefSeq:	XP_828072.1
UniProt:	Q387R2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDIPQELACP TEDRRLYVVR GQTFEVGRSH KLVKFIGSGS YGSVCSAVDE DSGERVAIKR IQRVFSDLKE GKRILREMDI LTSLHHTNLV RLRQFIRPVR KDDFSDIYIV MDLYDTDLHR IIRSRQSLTN EHYQYLMIQA FRGLFYLHTA KVIHRDLKPS NLLVNADCTL AICDFGLARD DQCTQTSALT EYVVTRWYRP PEVLGMGSHR YTNAVDVWSL GLIFAELMVG KTLLPGSDYI HQLIMIVHLL GTPASEDMEF LSEEAKNFLL AQPFQEARPF TELFPAATPE AADLLSKLLV FHPDKRLTAK EVIEHAYFAK FRNPAAETGA KEPYVWRHNE GLTLEGLREE MWRIILAHSP GPKGD

NT Sequence

atggacatac cacaagaact ggcttgtcct acggaagatc gacgtttata cgttgtccgg gggcaaacat ttgaagttgg ccgttctcat aaactcgtga aatttattgg atccgggtcg tacgggtctg tgtgctcggc tgttgatgaa gatagcggcg aacgtgtcgc tatcaaaaga atacaacgcg tattctcaga cttaaaggaa ggcaagcgta tattgaggga aatggatatt cttacatcgc tacatcacac caacctcgta cgcctaaggc agtttattcg cccagtacgg aaagacgatt tttctgatat atacatagtg atggaccttt acgacacgga tcttcaccgg atcatccgaa gtcgacaaag tcttacaaac gaacattatc agtacctaat gattcaggcg tttcgtgggc ttttctactt gcacaccgca aaagttatac accgtgactt aaaaccaagt aatttattag ttaacgctga ttgtactctg gctatctgtg acttcgggct tgctcgtgat gatcaatgta cgcagacctc tgcgctcacc gaatatgttg taactaggtg gtatcgacca cctgaagtgt taggcatggg atcccatcga tatacaaacg cagtggatgt ctggagccta gggctcatct ttgctgaatt gatggttggt aagacgcttt tgccgggctc tgattacatt caccaattga taatgattgt ccatctccta gggacccccg cttcggagga tatggaattt cttagcgagg aagcaaagaa ctttcttctt gcacagcctt ttcaggaggc tcgaccgttt acagaactct ttcccgctgc tactcctgag gcggctgatc tactttcaaa gctgctagta ttccatcctg acaagcggct tacggcaaag gaggtgattg aacatgcgta ttttgctaag tttaggaacc ctgcggcgga gacaggtgct aaagagccgt atgtttggcg acacaatgag ggactcacac ttgagggcct tcgtgaggaa atgtggcgta ttattttggc acattctccc ggtccaaagg gtgactag

Details for TrbrB.01003.b.B1.GE36990

HARVESTED ON:	5/21/2013
SEQUENCED ON:	5/23/2013
EXPECTED MW:	43kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	85

Validated AA Sequence

MAHHHHHHMD IPQELACPTE DRRLYVVRGQ TFEVGRSHKL VKFIGSGSYG SVCSAVDEDS GERVAIKRIQ RVFSDLKEGK RILREMDILT SLHHANLVRL RQFIRPVRKD DFSDIYIVMD LYDTDLHRII RSRQSLTNEH YQYLMIQAFR GLFYLHTAKV IHRDLKPSNL LVNADCTLAI CDFGLARDDQ CTQTSALTEY VVTRWYRPPE VLGMGSHRYT NAVDVWSLGL IFAELMVGKT LLPGSDYIHQ LIMIVHLLXT PASXDMEFLS EEAKNFLLAQ PFXEARPFTE LFPAATPXAA DLLSKLLVFH PXSGLRQXXD

Validated NT Sequence

attttgttta actttaagaa ggaganatac catggctcac caccaccacc accatatgga cataccacaa gaactggctt gtcctacgga agatcgacgt ttatacgttg tccgggggca aacatttgaa gttggccgtt ctcataaact cgtgaaattt attggatccg ggtcgtacgg gtctgtgtgc tcggctgttg atgaagatag cggcgaacgt gtcgctatca aaagaataca acgcgtattc tcagacttaa aggaaggcaa gcgtatattg agggaaatgg atattcttac atcgctacat cacgccaacc tcgtacgcct aaggcagttt attcgcccag tacggaaaga cgatttttct gatatataca tagtgatgga cctttacgac acggatcttc accggatcat ccgaagtcga caaagtctta caaacgaaca ttatcagtac ctaatgattc aggcgtttcg tgggcttttc tacttgcaca ccgcaaaagt tatacaccgt gacttaaaac caagtaattt attagttaac gctgattgta ctctggctat ctgtgacttc gggcttgctc gtgatgatca atgtacgcag acctctgcgc tcaccgaata tgttgtaact aggtggtatc gaccacctga agtgttaggc atgggatccc atcgatatac aaacgcagtg gatgtctgga gcctagggct catctttgct gaattgatgg ttggtaagac gcttttgcct ggctctgatt acattcatca attgataatg attgtccatc tcctanggac ccccgcttcn gangatatgg aatttcttag cgaggaagca aagaactttc ttcttgcaca gccttttcng gaggctcgac cgtttacnga actctttccc gctgctactc ctgangcggc tgatctactt tcaaagctgc tagtattcca tcctganagc ggcttacggc aaagnnnnga ttgaanntgc ntattttnct aagttagnan cntgnnncng aacngngcta anagccntnn gttnnnnacn nnnngaggnn tnnnnntnag gctnnnnnng naatgnngnn gtantanttn nnnnntnnnn nnnnnnannn nnnnnnnnnn nnnnnnnnnn c

Expected Protein Sequence

MAHHHHHHMD IPQELACPTE DRRLYVVRGQ TFEVGRSHKL VKFIGSGSYG SVCSAVDEDS GERVAIKRIQ RVFSDLKEGK RILREMDILT SLHHTNLVRL RQFIRPVRKD DFSDIYIVMD LYDTDLHRII RSRQSLTNEH YQYLMIQAFR GLFYLHTAKV IHRDLKPSNL LVNADCTLAI CDFGLARDDQ CTQTSALTEY VVTRWYRPPE VLGMGSHRYT NAVDVWSLGL IFAELMVGKT LLPGSDYIHQ LIMIVHLLGT PASEDMEFLS EEAKNFLLAQ PFQEARPFTE LFPAATPEAA DLLSKLLVFH PDKRLTAKEV IEHAYFAKFR NPAAETGAKE PYVWRHNEGL TLEGLREEMW RIILAHSPGP KGD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaca taccacaaga actggcttgt cctacggaag atcgacgttt atacgttgtc cgggggcaaa catttgaagt tggccgttct cataaactcg tgaaatttat tggatccggg tcgtacgggt ctgtgtgctc ggctgttgat gaagatagcg gcgaacgtgt cgctatcaaa agaatacaac gcgtattctc agacttaaag gaaggcaagc gtatattgag ggaaatggat attcttacat cgctacatca caccaacctc gtacgcctaa ggcagtttat tcgcccagta cggaaagacg atttttctga tatatacata gtgatggacc tttacgacac ggatcttcac cggatcatcc gaagtcgaca aagtcttaca aacgaacatt atcagtacct aatgattcag gcgtttcgtg ggcttttcta cttgcacacc gcaaaagtta tacaccgtga cttaaaacca agtaatttat tagttaacgc tgattgtact ctggctatct gtgacttcgg gcttgctcgt gatgatcaat gtacgcagac ctctgcgctc accgaatatg ttgtaactag gtggtatcga ccacctgaag tgttaggcat gggatcccat cgatatacaa acgcagtgga tgtctggagc ctagggctca tctttgctga attgatggtt ggtaagacgc ttttgccggg ctctgattac attcaccaat tgataatgat tgtccatctc ctagggaccc ccgcttcgga ggatatggaa tttcttagcg aggaagcaaa gaactttctt cttgcacagc cttttcagga ggctcgaccg tttacagaac tctttcccgc tgctactcct gaggcggctg atctactttc aaagctgcta gtattccatc ctgacaagcg gcttacggca aaggaggtga ttgaacatgc gtattttgct aagtttagga accctgcggc ggagacaggt gctaaagagc cgtatgtttg gcgacacaat gagggactca cacttgaggg ccttcgtgag gaaatgtggc gtattatttt ggcacattct cccggtccaa agggtgactg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat