SSGCID: Target Page

TrbrB.00437.a

Protein kinase, putative

CENTER ID:	TrbrB.00437.a
ORGANISM:	Trypanosoma brucei GUTat10.1
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrB.00437.a.B1.GE37077	full length		1	570
TrbrB.00437.a.B2.GE37078	protein kinase domain		11	389

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TritrypDB:Tb927.10.10870
RefSeq:	XP_823305.1
UniProt:	Q389P3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHNVPLLAWR YEHVAVVGKG GFGIVSEHVD RFTGEHVAVK TIPSRFVNQE SARLVREIDI MSFLHDAHPH VIGYFDMFAT PSGADNVLSQ DEGGRDGATT LVDARRFDAA GPQYPNQSRN DELSKYHDKV LSMVNRLSGE DEFSLHIVMP LMKGDLLYFI KHVSSSGSKP AMTDEFIELV TVVFAFQICF GLDFLHKCNI VHRDIKPENI LVRLHGKDPY KSTALIADFG LARDAQASDT FYVCTRYYRP PEIVTNVSGG EPSIDVWSVG CILFEMVTGK ALFNVESALN EQGVWDGQLA STQLEVILNI VGTPSHDDIR RFMPVGNAQN YLLRSAPRPS RLVGMMNEQW RLHTTRDRQQ KWIDLISSCL AFFPQQRPTC GDLCRHELFQ EYNLFYGGNV MQYQPKRYQS AEVNTLKTKN KQSVLHLVRL ALQKHSPVVD VSFSDGAKWK DEDEEEGKKS PCAPKERFLD EGELLATPEA VGSDVQDSDM RGEGVTPLEG GSEEGELGES IAFRFLKDSE LRRQYDNWGW SGRTRDEVLA KILGDLQRYT HDAVRSEQLR ELLRHFSSPR

NT Sequence

atgcacaatg tcccgttact cgcttggcgg tatgagcacg ttgcggtcgt tggcaagggt ggattcggta ttgtgagcga gcacgtagat cgtttcacag gagaacatgt tgccgtgaag acaatccctt cgcgctttgt aaaccaggaa agcgcccgcc tcgttagaga gatagatata atgtctttcc tccatgacgc tcatcctcac gtgattgggt actttgacat gtttgcgacc ccaagcggtg ctgacaacgt gctctctcag gatgaaggtg gtagggacgg ggcaacgaca ctggtggacg cccgccgctt cgacgccgca gggcctcaat accctaatca aagccgcaat gacgagcttt ccaaatacca tgataaagtc ctttctatgg tgaatcgctt gagcggagag gacgaattta gtctccatat agtgatgcct ctgatgaagg gtgacttgct ctattttata aagcacgtat catcttcagg cagtaaaccg gccatgacag atgaattcat tgagctggtc actgttgttt ttgcatttca gatatgtttt ggtttggatt ttttgcacaa gtgtaatatc gtccacagag atataaagcc agaaaatatt ttggttcgtt tgcatggtaa agatccatac aaatcaacgg cgctcatagc cgattttggc cttgcacggg acgcccaagc aagtgacacc ttttatgtct gcacgcgcta ttacaggcca cctgagatcg ttaccaatgt ctcgggaggg gagccgtcga ttgatgtgtg gtcggtgggt tgcatccttt tcgaaatggt tacagggaaa gctttgttta atgttgaatc cgcccttaat gaacaagggg tgtgggatgg acaacttgct agcacacaac tagaagtgat tcttaacatt gttgggacac ctagccatga tgatatacga agattcatgc ctgtgggaaa cgctcaaaat tatctcctcc ggagcgctcc acgtccctca cgattggttg gaatgatgaa cgagcagtgg cgattgcaca caacgcggga tcggcagcag aaatggattg atttgatatc cagctgcctt gcctttttcc ctcagcagcg tcccacctgt ggagatctct gtcgccacga actttttcaa gaatataatc ttttctatgg tggaaacgtg atgcagtacc aaccgaaaag atatcagtcc gctgaggtga acactttgaa gacgaaaaac aagcagagtg ttttgcatct tgtgcgactt gcgctgcaga agcattcgcc ggttgtcgat gtaagtttct ccgatggtgc gaagtggaag gatgaggatg aggaagaagg gaagaagagc ccgtgtgccc ccaaagaaag atttttggat gaaggtgagt tgttggcaac cccagaagca gtgggaagtg atgtacaaga ctccgacatg aggggcgaag gcgtcacgcc attggaaggg ggcagtgaag agggcgagtt gggggaatca attgcgtttc ggtttctcaa ggattcggag ctccggaggc aatacgacaa ttggggatgg tcaggaagga ctcgcgatga ggtgcttgca aagatcttag gagacctcca gcgttacacg cacgacgccg tacgttctga gcaactaaga gaacttctga ggcacttttc atcacctcgt taa

Details for TrbrB.00437.a.B1.GE37077

HARVESTED ON:	data unavailable
SEQUENCED ON:	6/11/2013
EXPECTED MW:	65kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMH NVPLLAWRYE HVAVVGKGGF GIVSEHVDRF TGEHVAVKTI PSRFVNQESA RLVREIDIMS FLHDAHPHVI GYFDMFATPS GADNVLSQDE GGRDGATTLV DARRFDAAGP QYPNQSRNDE LSKYHDKVLS MVNRLSGEDE FSLHIVMPLM KGDLLYFIKH VSSSGSKPAM TDEFIELVTV VFAFQICFGL DFLHKCNIVH RDIKPENILV RLHGKDPYKS TALIADFGLA RDAQASDTFY VCTRYYRPPE IVTNVSGGEP SIDVWSVGCI LFEMVTGKAL FNVESALNEQ GVWDGQLAST QLEVILNIVG TPSHDDIRRF MPVGNAQNYL LRSAPRPSRL VGMMNEQWRL HTTRDRQQKW IDLISSCLAF FPQQRPTCGD LCRHELFQEY NLFYGGNVMQ YQPKRYQSAE VNTLKTKNKQ SVLHLVRLAL QKHSPVVDVS FSDGAKWKDE DEEEGKKSPC APKERFLDEG ELLATPEAVG SDVQDSDMRG EGVTPLEGGS EEGELGESIA FRFLKDSELR RQYDNWGWSG RTRDEVLAKI LGDLQRYTHD AVRSEQLREL LRHFSSPR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaca atgtcccgtt actcgcttgg cggtatgagc acgttgcggt cgttggcaag ggtggattcg gtattgtgag cgagcacgta gatcgtttca caggagaaca tgttgccgtg aagacaatcc cttcgcgctt tgtaaaccag gaaagcgccc gcctcgttag agagatagat ataatgtctt tcctccatga cgctcatcct cacgtgattg ggtactttga catgtttgcg accccaagcg gtgctgacaa cgtgctctct caggatgaag gtggtaggga cggggcaacg acactggtgg acgcccgccg cttcgacgcc gcagggcctc aataccctaa tcaaagccgc aatgacgagc tttccaaata ccatgataaa gtcctttcta tggtgaatcg cttgagcgga gaggacgaat ttagtctcca tatagtgatg cctctgatga agggtgactt gctctatttt ataaagcacg tatcatcttc aggcagtaaa ccggccatga cagatgaatt cattgagctg gtcactgttg tttttgcatt tcagatatgt tttggtttgg attttttgca caagtgtaat atcgtccaca gagatataaa gccagaaaat attttggttc gtttgcatgg taaagatcca tacaaatcaa cggcgctcat agccgatttt ggccttgcac gggacgccca agcaagtgac accttttatg tctgcacgcg ctattacagg ccacctgaga tcgttaccaa tgtctcggga ggggagccgt cgattgatgt gtggtcggtg ggttgcatcc ttttcgaaat ggttacaggg aaagctttgt ttaatgttga atccgccctt aatgaacaag gggtgtggga tggacaactt gctagcacac aactagaagt gattcttaac attgttggga cacctagcca tgatgatata cgaagattca tgcctgtggg aaacgctcaa aattatctcc tccggagcgc tccacgtccc tcacgattgg ttggaatgat gaacgagcag tggcgattgc acacaacgcg ggatcggcag cagaaatgga ttgatttgat atccagctgc cttgcctttt tccctcagca gcgtcccacc tgtggagatc tctgtcgcca cgaacttttt caagaatata atcttttcta tggtggaaac gtgatgcagt accaaccgaa aagatatcag tccgctgagg tgaacacttt gaagacgaaa aacaagcaga gtgttttgca tcttgtgcga cttgcgctgc agaagcattc gccggttgtc gatgtaagtt tctccgatgg tgcgaagtgg aaggatgagg atgaggaaga agggaagaag agcccgtgtg cccccaaaga aagatttttg gatgaaggtg agttgttggc aaccccagaa gcagtgggaa gtgatgtaca agactccgac atgaggggcg aaggcgtcac gccattggaa gggggcagtg aagagggcga gttgggggaa tcaattgcgt ttcggtttct caaggattcg gagctccgga ggcaatacga caattgggga tggtcaggaa ggactcgcga tgaggtgctt gcaaagatct taggagacct ccagcgttac acgcacgacg ccgtacgttc tgagcaacta agagaacttc tgaggcactt ttcatcacct cgttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for TrbrB.00437.a.B2.GE37078

HARVESTED ON:	6/12/2013
SEQUENCED ON:	6/11/2013
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	82
PERCENT COVERAGE:	102

Validated AA Sequence

MAHHHHHHMY EHVAVVGKGG FGIVSEHVDR FTGEHVAVKT IPSRFVNQES ARLVREIDIM SFLHDAHPHV IGYFDMFATP SGADNVLSQD EGGGDGATTV VDARRFDAAG PQYPNLSRND ELSKYHDKVL SMVNRLSGED EFSLHIVMPL MKGDLLYFIK HVSSSGSKPT MTDEFIELVT VVFAFQICFG LDFLHKCNIV HRDIKPENIL VRLHGKDPYK STALIADFGL ARDAQASDTF YVCTRYYRPP EIVTNVSGGX PSIDVWSVGC ILFXMVTGKA LFNVESALNE QGVWXGXLAS TQLEVIXXIV GXPSXDDIXX FMPVGNXQNX XLXXXXRPXX LGWXDXXXWX XXXTXGSXAX XDXXXXXXXX FXXXXXXXXX XXXXXXXXXX XXXXXX

Validated NT Sequence

anttttgttt aactttaaga nggaganata ccatggctca ccaccaccac caccatatgt atgagcacgt tgcggtcgtt ggcaagggtg gattcggtat tgtgagcgag cacgtagatc gtttcacagg agaacatgtt gccgtgaaga caatcccttc gcgctttgta aaccaggaga gcgcccgcct cgttagagag atagatataa tgtctttcct ccatgacgct catcctcacg tgattgggta ctttgacatg tttgcgaccc caagcggtgc tgacaacgtg ctctcccagg atgaaggcgg tggggacggg gcaacgacag tggtggacgc ccgccgcttc gacgccgcag ggcctcaata ccctaatcta agccgcaatg acgagctttc caaataccat gataaagtcc tttctatggt gaatcgcttg agcggagagg acgaatttag tctccatata gtgatgcctc tgatgaaggg tgacttgctc tattttataa agcacgtatc atcttcaggc agtaaaccga ccatgacaga tgaattcatt gagctggtca ctgttgtttt tgcatttcag atatgttttg gtttggattt tttgcacaag tgtaatatcg tccacagaga tataaagcca gaaaatattt tggttcgttt gcatggtaaa gatccataca aatcaacggc gctcatagcc gattttggcc ttgcacggga cgcccaagca agtgacacct tttatgtctg cacgcgctat tacaggccac ctgagatcgt taccaatgtc tcgggagggn agccgtcgat tgatgtgtgg tcggtgggtt gcatcctttt cnaaatggtt acagggaaag ctttgtttaa tgttgaatcc gcccttaatg aacaaggggt gtgggangga cnacttgcta gcacacaact agaagtgatt cntnacattg ttggganacc tagccntgat gatatacnaa nattcatgcc tgtgggnaac nntcaaaatt annnnctncn nannnntncn cgtccctnnn nattgggttg gnatgatgan gannnntggn natgnnnnnn nacnnnngga tcnncagcnn aaanggatnn ntngannncn nntnnnnnnn nnttttncnn nnncagnnnc cnnnnnnnng nanncnccnn nnntttnnnn nnnnnnannn nnnncngnnn nnnnnnnana a

Expected Protein Sequence

MAHHHHHHYE HVAVVGKGGF GIVSEHVDRF TGEHVAVKTI PSRFVNQESA RLVREIDIMS FLHDAHPHVI GYFDMFATPS GADNVLSQDE GGRDGATTLV DARRFDAAGP QYPNQSRNDE LSKYHDKVLS MVNRLSGEDE FSLHIVMPLM KGDLLYFIKH VSSSGSKPAM TDEFIELVTV VFAFQICFGL DFLHKCNIVH RDIKPENILV RLHGKDPYKS TALIADFGLA RDAQASDTFY VCTRYYRPPE IVTNVSGGEP SIDVWSVGCI LFEMVTGKAL FNVESALNEQ GVWDGQLAST QLEVILNIVG TPSHDDIRRF MPVGNAQNYL LRSAPRPSRL VGMMNEQWRL HTTRDRQQKW IDLISSCLAF FPQQRPTCGD LCRHELF

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattatgagc acgttgcggt cgttggcaag ggtggattcg gtattgtgag cgagcacgta gatcgtttca caggagaaca tgttgccgtg aagacaatcc cttcgcgctt tgtaaaccag gaaagcgccc gcctcgttag agagatagat ataatgtctt tcctccatga cgctcatcct cacgtgattg ggtactttga catgtttgcg accccaagcg gtgctgacaa cgtgctctct caggatgaag gtggtaggga cggggcaacg acactggtgg acgcccgccg cttcgacgcc gcagggcctc aataccctaa tcaaagccgc aatgacgagc tttccaaata ccatgataaa gtcctttcta tggtgaatcg cttgagcgga gaggacgaat ttagtctcca tatagtgatg cctctgatga agggtgactt gctctatttt ataaagcacg tatcatcttc aggcagtaaa ccggccatga cagatgaatt cattgagctg gtcactgttg tttttgcatt tcagatatgt tttggtttgg attttttgca caagtgtaat atcgtccaca gagatataaa gccagaaaat attttggttc gtttgcatgg taaagatcca tacaaatcaa cggcgctcat agccgatttt ggccttgcac gggacgccca agcaagtgac accttttatg tctgcacgcg ctattacagg ccacctgaga tcgttaccaa tgtctcggga ggggagccgt cgattgatgt gtggtcggtg ggttgcatcc ttttcgaaat ggttacaggg aaagctttgt ttaatgttga atccgccctt aatgaacaag gggtgtggga tggacaactt gctagcacac aactagaagt gattcttaac attgttggga cacctagcca tgatgatata cgaagattca tgcctgtggg aaacgctcaa aattatctcc tccggagcgc tccacgtccc tcacgattgg ttggaatgat gaacgagcag tggcgattgc acacaacgcg ggatcggcag cagaaatgga ttgatttgat atccagctgc cttgcctttt tccctcagca gcgtcccacc tgtggagatc tctgtcgcca cgaacttttt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat