TrbrB.00436.c

Lapatinib-binding protein kinase 3 (LBPK3) (OPH8)

CENTER ID: TrbrB.00436.c
ORGANISM: Trypanosoma brucei GUTat10.1
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrB.00436.c.B1.GE37074 full length 1 622
TrbrB.00436.c.B2.GE37075 kinase domain, C-term extended 104 622
TrbrB.00436.c.B3.GE37076 protein kinase domain 104 488
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tb927.3.1570
RefSeq: XP_843751.1
UniProt: Q57XZ0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGHWEGYGT PTMSEGSISP CIRSRSNSRG VLVRSNSPGC GELAIVPPQF ELKLAMKQND PFLYNSCQSS FGPLSVPTVC PTCHRPLAEN QMDDAPPTVG ERQLTPQYFR ALAATVTRRP LSICDSPSLE AKFQQRLAEQ GLKKYVPEDE IRGKMGVTEE EEDTWELPDE AHGATAVFSS ALDENSRRGG GPETSPPRGD NTSGDWGYYK RYFREIQKLG SGTFGGVYLC QHVMEGVGLG YFALKKIPVG DNTSYLHKVL SEVRILEEVK QHPNMVEYKH SWVDTAQIAD FGPPVRCLFI LMEYATIGSL DSYLERHGSA LPTIAVWYFF LSAVAGTAHL HRKNIIHRDL KPQNLLLTGR SEEVPRVLVS DFGTATLLSE VSYERTGGTG TVEYMAPELF ECVPGTKESY IYSHTKATDV WSLGMILHYL AFDGTLPLRL SNGEVVLEVE NRSHYARPPE MIQLMRLMLH RNPAKRPTCE ELLESTVVRT IQRSFKKASL FTDILPTAAT LPKRRRPSAV LTTPLLTPRE GKDSQARQLR VNWVANNTAK GELEERKPTP CLDSEVGNEP LPLFLPKAEV PLMLQYETRG SHGETTEGAQ GMQKPLMVEC GVQTDYVKIV YE
NT Sequence
atgagtggcc attgggaggg gtatggcaca cccactatga gtgaaggttc cattagccct tgtatcagaa gcaggagcaa cagcagagga gttttggttc gcagtaactc tccgggatgt ggtgagcttg ccatagttcc accacagttt gagcttaaat tagcaatgaa acaaaatgat ccattccttt ataattcttg tcaatcatca tttggtccct tgtccgtgcc cacggtttgc ccaacatgtc atcggcctct cgctgaaaat cagatggatg atgcaccgcc aacggtgggg gagagacaac taactccaca atacttccgt gcacttgctg cgactgttac tcgtcggccg ctttccatat gtgacagtcc atcacttgaa gctaaattcc agcaacggtt ggctgaacag ggtttgaaga aatatgtccc ggaagatgaa ataagaggaa aaatgggagt tacagaagaa gaagaagaca cgtgggaact acccgatgag gcacatggag caacagcggt gttttcgtcg gcgttagatg aaaacagcag aaggggcggc ggacctgaaa cgtctcctcc acgcggtgat aataccagcg gtgactgggg gtattataag cgttacttta gagaaataca aaaactagga agcggaacct ttggtggagt gtatctgtgt cagcatgtta tggaaggcgt ggggttgggg tactttgctt taaagaaaat tcccgtagga gacaatacca gttacttgca caaagtactc agtgaggtgc gtattctcga ggaagtgaaa caacacccaa acatggtgga gtataagcat agctgggtag ataccgcgca aatagctgat tttgggccac ccgtaagatg cctttttatt cttatggaat atgccactat aggttccctt gactcgtacc ttgagcggca cggatcggct ctgccaacaa tcgccgtatg gtacttcttt ttgagtgccg ttgcggggac agcgcatttg catcgaaaga acatcatcca tcgggacctc aagccacaaa atttactgct gacaggtcgg tcggaggagg tcccacgtgt tcttgttagc gactttggca ctgcaacttt actttctgag gtgtcatatg aacgcacggg aggaacggga accgtagagt acatggcacc agagttattt gagtgtgtgc cgggcacgaa agagtcatat atatattcgc acacaaaagc aacagatgtg tggtcactcg gaatgatatt acactatctt gccttcgacg gtacgctgcc gctgcgcttg tctaatggtg aagttgtatt ggaagtggag aaccgttccc actacgctcg ccctccagag atgattcaac taatgcgcct catgcttcat cgtaacccag cgaagcgacc cacatgtgag gaattgttag aatcgactgt agtgcgaaca attcagcgta gtttcaagaa ggcaagtctt tttaccgata tattacctac tgcagccacg cttccgaaac gtcggcgacc atccgctgtt ctgacaacac cgctgctgac acctcgtgaa gggaaagact cgcaagccag acaacttcga gttaattggg tcgcaaataa caccgcaaag ggtgaactag aagagaggaa gccaacacct tgtttggata gcgaagttgg aaatgagcca ctgccattat ttttgcctaa agcggaagtc cccctaatgc ttcaatatga aactcgtggg tcacatggag agacaacaga gggggcgcaa gggatgcaga aaccactgat ggtagaatgt ggtgtgcaaa ctgactacgt aaagattgtg tacgagtga
Details for TrbrB.00436.c.B1.GE37074
HARVESTED ON: 6/12/2013
SEQUENCED ON: 6/11/2013
EXPECTED MW: 70kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 82
PERCENT COVERAGE: 62
Validated AA Sequence
MAHHHHHHMS GHWEGYGTPT MSEGSISPCI RSRSNSRGVL VRSNSPGCGE LAIVPPQFEL KLAMKQNDPF LYNSCQSSFG PLSVPTVCPT CHRPLAENQM DDAPPTVGER QLTPQYFRAL AATVTRRPLS ICDSPSLEAK FQQRLAEQGL KKYVPEDEIR GKMGVTEEEE DTWELPDEAH GATAVFSSAL DENSRRGGGP ETSPPRGDNT SGDWGYYKRY FREIQKLGSG TFGGVYLCQH VMEGVXLGYF ALKKIPVXDN TSYLHKVLSE VRILEEVKQX PNMVXYXHSW VDTAQIAXFG PPVXCLFXXX XXATIGXXDS YLERHXXALP TIAXWXFFXX XXAGTXXXIX RXXXXXXXXK XLLXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXX
Validated NT Sequence
anttttgttt aactttaaga aggagatata ccatggctca ccaccaccac caccatatga gtggccattg ggaggggtat ggcacaccca ctatgagtga aggttccatt agcccttgta tcagaagcag gagcaacagc agaggagttt tggttcgcag taactctccg ggatgtggtg agcttgccat agttccacca cagtttgagc ttaaattagc aatgaaacaa aatgatccat tcctttataa ttcttgtcaa tcatcatttg gtcccttgtc cgtgcccacg gtttgcccaa catgtcatcg gcctctcgct gaaaatcaga tggatgatgc accgccaacg gtgggggaga gacaactaac tccacaatac ttccgtgcac ttgctgcgac tgttactcgt cggccgcttt ccatatgtga cagtccatca cttgaagcta aattccagca acggttggct gaacagggtt tgaagaaata tgtcccggaa gatgaaataa gaggaaaaat gggagttaca gaagaagaag aggacacgtg ggaactaccc gatgaggcac atggagcaac agcggtgttt tcgtcggcgt tagatgaaaa cagcagaagg ggcggcggac ctgaaacgtc tcctccacgg ggtgataata ccagcggtga ctgggggtat tataagcgtt actttagaga aatacaaaaa ctaggaagcg gaacctttgg tggagtgtat ctgtgtcagc atgttatgga aggcgtgngg ttggggtact ttgctttaaa gaaaattccc gtangagaca ataccagtta cttgcacaaa gtactcagtg aggtgcgtat tctcgaggaa gtgaaacaac ncccaaacat ggtggantat aancatagct gggtagatac cgcgcaaata gctgantttg ggccacccgt aanatgcctt tttantcnna nngannatgc cactataggt tncnnngact cgtaccttga gcggcacngn ncggctctgc caacaatcgc cgnatggnac ttcttttnna nngncnttgc ggggacngnn nnnngcatcn aaaganntcn tcntcngnnn ncancnnnaa aantnctgct gannnnngnc gnnngnnnnc nnnnnnnntn nnnnnanttn nnnnnnnann ttnnntnnnn nnnnnnnnnn nannnnngnn nnnaangnnn cnnnnnna
Expected Protein Sequence
MAHHHHHHMS GHWEGYGTPT MSEGSISPCI RSRSNSRGVL VRSNSPGCGE LAIVPPQFEL KLAMKQNDPF LYNSCQSSFG PLSVPTVCPT CHRPLAENQM DDAPPTVGER QLTPQYFRAL AATVTRRPLS ICDSPSLEAK FQQRLAEQGL KKYVPEDEIR GKMGVTEEEE DTWELPDEAH GATAVFSSAL DENSRRGGGP ETSPPRGDNT SGDWGYYKRY FREIQKLGSG TFGGVYLCQH VMEGVGLGYF ALKKIPVGDN TSYLHKVLSE VRILEEVKQH PNMVEYKHSW VDTAQIADFG PPVRCLFILM EYATIGSLDS YLERHGSALP TIAVWYFFLS AVAGTAHLHR KNIIHRDLKP QNLLLTGRSE EVPRVLVSDF GTATLLSEVS YERTGGTGTV EYMAPELFEC VPGTKESYIY SHTKATDVWS LGMILHYLAF DGTLPLRLSN GEVVLEVENR SHYARPPEMI QLMRLMLHRN PAKRPTCEEL LESTVVRTIQ RSFKKASLFT DILPTAATLP KRRRPSAVLT TPLLTPREGK DSQARQLRVN WVANNTAKGE LEERKPTPCL DSEVGNEPLP LFLPKAEVPL MLQYETRGSH GETTEGAQGM QKPLMVECGV QTDYVKIVYE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtg gccattggga ggggtatggc acacccacta tgagtgaagg ttccattagc ccttgtatca gaagcaggag caacagcaga ggagttttgg ttcgcagtaa ctctccggga tgtggtgagc ttgccatagt tccaccacag tttgagctta aattagcaat gaaacaaaat gatccattcc tttataattc ttgtcaatca tcatttggtc ccttgtccgt gcccacggtt tgcccaacat gtcatcggcc tctcgctgaa aatcagatgg atgatgcacc gccaacggtg ggggagagac aactaactcc acaatacttc cgtgcacttg ctgcgactgt tactcgtcgg ccgctttcca tatgtgacag tccatcactt gaagctaaat tccagcaacg gttggctgaa cagggtttga agaaatatgt cccggaagat gaaataagag gaaaaatggg agttacagaa gaagaagaag acacgtggga actacccgat gaggcacatg gagcaacagc ggtgttttcg tcggcgttag atgaaaacag cagaaggggc ggcggacctg aaacgtctcc tccacgcggt gataatacca gcggtgactg ggggtattat aagcgttact ttagagaaat acaaaaacta ggaagcggaa cctttggtgg agtgtatctg tgtcagcatg ttatggaagg cgtggggttg gggtactttg ctttaaagaa aattcccgta ggagacaata ccagttactt gcacaaagta ctcagtgagg tgcgtattct cgaggaagtg aaacaacacc caaacatggt ggagtataag catagctggg tagataccgc gcaaatagct gattttgggc cacccgtaag atgccttttt attcttatgg aatatgccac tataggttcc cttgactcgt accttgagcg gcacggatcg gctctgccaa caatcgccgt atggtacttc tttttgagtg ccgttgcggg gacagcgcat ttgcatcgaa agaacatcat ccatcgggac ctcaagccac aaaatttact gctgacaggt cggtcggagg aggtcccacg tgttcttgtt agcgactttg gcactgcaac tttactttct gaggtgtcat atgaacgcac gggaggaacg ggaaccgtag agtacatggc accagagtta tttgagtgtg tgccgggcac gaaagagtca tatatatatt cgcacacaaa agcaacagat gtgtggtcac tcggaatgat attacactat cttgccttcg acggtacgct gccgctgcgc ttgtctaatg gtgaagttgt attggaagtg gagaaccgtt cccactacgc tcgccctcca gagatgattc aactaatgcg cctcatgctt catcgtaacc cagcgaagcg acccacatgt gaggaattgt tagaatcgac tgtagtgcga acaattcagc gtagtttcaa gaaggcaagt ctttttaccg atatattacc tactgcagcc acgcttccga aacgtcggcg accatccgct gttctgacaa caccgctgct gacacctcgt gaagggaaag actcgcaagc cagacaactt cgagttaatt gggtcgcaaa taacaccgca aagggtgaac tagaagagag gaagccaaca ccttgtttgg atagcgaagt tggaaatgag ccactgccat tatttttgcc taaagcggaa gtccccctaa tgcttcaata tgaaactcgt gggtcacatg gagagacaac agagggggcg caagggatgc agaaaccact gatggtagaa tgtggtgtgc aaactgacta cgtaaagatt gtgtacgagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for TrbrB.00436.c.B2.GE37075
HARVESTED ON: 6/12/2013
SEQUENCED ON: 6/11/2013
EXPECTED MW: 59kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHLT PQYFRALAAT VTRRPLSICD SPSLEAKFQQ RLAEQGLKKY VPEDEIRGKM GVTEEEEDTW ELPDEAHGAT AVFSSALDEN SRRGGGPETS PPRGDNTSGD WGYYKRYFRE IQKLGSGTFG GVYLCQHVME GVGLGYFALK KIPVGDNTSY LHKVLSEVRI LEEVKQHPNM VEYKHSWVDT AQIADFGPPV RCLFILMEYA TIGSLDSYLE RHGSALPTIA VWYFFLSAVA GTAHLHRKNI IHRDLKPQNL LLTGRSEEVP RVLVSDFGTA TLLSEVSYER TGGTGTVEYM APELFECVPG TKESYIYSHT KATDVWSLGM ILHYLAFDGT LPLRLSNGEV VLEVENRSHY ARPPEMIQLM RLMLHRNPAK RPTCEELLES TVVRTIQRSF KKASLFTDIL PTAATLPKRR RPSAVLTTPL LTPREGKDSQ ARQLRVNWVA NNTAKGELEE RKPTPCLDSE VGNEPLPLFL PKAEVPLMLQ YETRGSHGET TEGAQGMQKP LMVECGVQTD YVKIVYE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctaactc cacaatactt ccgtgcactt gctgcgactg ttactcgtcg gccgctttcc atatgtgaca gtccatcact tgaagctaaa ttccagcaac ggttggctga acagggtttg aagaaatatg tcccggaaga tgaaataaga ggaaaaatgg gagttacaga agaagaagaa gacacgtggg aactacccga tgaggcacat ggagcaacag cggtgttttc gtcggcgtta gatgaaaaca gcagaagggg cggcggacct gaaacgtctc ctccacgcgg tgataatacc agcggtgact gggggtatta taagcgttac tttagagaaa tacaaaaact aggaagcgga acctttggtg gagtgtatct gtgtcagcat gttatggaag gcgtggggtt ggggtacttt gctttaaaga aaattcccgt aggagacaat accagttact tgcacaaagt actcagtgag gtgcgtattc tcgaggaagt gaaacaacac ccaaacatgg tggagtataa gcatagctgg gtagataccg cgcaaatagc tgattttggg ccacccgtaa gatgcctttt tattcttatg gaatatgcca ctataggttc ccttgactcg taccttgagc ggcacggatc ggctctgcca acaatcgccg tatggtactt ctttttgagt gccgttgcgg ggacagcgca tttgcatcga aagaacatca tccatcggga cctcaagcca caaaatttac tgctgacagg tcggtcggag gaggtcccac gtgttcttgt tagcgacttt ggcactgcaa ctttactttc tgaggtgtca tatgaacgca cgggaggaac gggaaccgta gagtacatgg caccagagtt atttgagtgt gtgccgggca cgaaagagtc atatatatat tcgcacacaa aagcaacaga tgtgtggtca ctcggaatga tattacacta tcttgccttc gacggtacgc tgccgctgcg cttgtctaat ggtgaagttg tattggaagt ggagaaccgt tcccactacg ctcgccctcc agagatgatt caactaatgc gcctcatgct tcatcgtaac ccagcgaagc gacccacatg tgaggaattg ttagaatcga ctgtagtgcg aacaattcag cgtagtttca agaaggcaag tctttttacc gatatattac ctactgcagc cacgcttccg aaacgtcggc gaccatccgc tgttctgaca acaccgctgc tgacacctcg tgaagggaaa gactcgcaag ccagacaact tcgagttaat tgggtcgcaa ataacaccgc aaagggtgaa ctagaagaga ggaagccaac accttgtttg gatagcgaag ttggaaatga gccactgcca ttatttttgc ctaaagcgga agtcccccta atgcttcaat atgaaactcg tgggtcacat ggagagacaa cagagggggc gcaagggatg cagaaaccac tgatggtaga atgtggtgtg caaactgact acgtaaagat tgtgtacgag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for TrbrB.00436.c.B3.GE37076
HARVESTED ON: 6/12/2013
SEQUENCED ON: 6/11/2013
EXPECTED MW: 44kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 80
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML TPQYFRALAA TVTRRPLSIC DSPSLEAKFQ QRLAEQGLKK YVPEDEIRGK MGVTEEEEDT WELPDEAHGA TAVFSSALDE NSRRGGGPET SPPRGDNTSG DWGYYKRYFR EIQKLGSGTF GGVYLCQHVM EGVGLGYFAL KKIPVGDNTS YLHKVLSEVR ILEEVKQHPN MVEYKHSWVD TAQIADFGPP VRCLFILMEY ATIGSLDSYL ERHGSALPTI AVWYFFLSAV AGTAHLHRKN IIHRDLKPQN LLLTGRSEEV PRVLVSDFGT AALLSXVSYE RTGXTGTVEY MAXXLFECVP GTKXSXIXSH TKATXXXXXX MXXXSCXXRY AXAXLXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXX
Validated NT Sequence
ttgtnnnnnt taagaaggag anataccatg gctcaccacc accaccacca tatgctaact ccacaatact tccgtgcact tgctgcgact gttactcgtc ggccgctttc catatgtgac agtccatcac ttgaagctaa attccagcaa cggttggctg aacagggttt gaagaaatat gtcccggaag atgaaataag aggaaaaatg ggagttacag aagaagaaga ggacacgtgg gaactacccg atgaggcaca tggagcaaca gcggtgtttt cgtcggcgtt agatgaaaac agcagaaggg gcggcggacc tgaaacgtct cctccacggg gtgataatac cagcggtgac tgggggtatt ataagcgtta ctttagagaa atacaaaaac taggaagcgg aacctttggt ggagtgtatc tgtgtcagca tgttatggaa ggcgtggggt tggggtactt tgctttaaag aaaattcccg taggagacaa taccagttac ttgcacaaag tactcagtga ggtgcgtatt ctcgaggaag tgaaacaaca cccaaacatg gtggagtata agcatagctg ggtagatacc gcgcaaatag ctgattttgg gccacccgta agatgccttt ttattcttat ggaatatgcc actataggtt cccttgactc gtaccttgag cggcacggat cggctctgcc aacaatcgcc gtatggtact tctttttgag tgccgttgcg gggacagcgc atttgcatcg aaagaacatc atccatcggg acctcaagcc acaaaattta ctgctgacag gtcggtcgga ggaggtccca cgtgttcttg ttagcgactt tggcactgca gctttacttt ctgangtgtc atatgaacgc acgggnngaa cgggaaccgt agagtacatg gcannngant tatttgagtg tgtgccgggc acgaaagant catanatann ntcncacaca aaagcaacag anntgnngnc antcngnatg nnnntnntat cttgcntnnn acggtacgct ncngctgnnc tngnnnnang gnngnnntnn ngtnnnnann nnnnccnnna cnctcnncnn nnnnnannac annannnnnn nnngnnnnnn ncnnnnnnnn nnnnnnnnnn nnnnnnnngn annnntnnaa nnnnnnnnnn nnnnnaannn nnnnnnnnan nnng
Expected Protein Sequence
MAHHHHHHLT PQYFRALAAT VTRRPLSICD SPSLEAKFQQ RLAEQGLKKY VPEDEIRGKM GVTEEEEDTW ELPDEAHGAT AVFSSALDEN SRRGGGPETS PPRGDNTSGD WGYYKRYFRE IQKLGSGTFG GVYLCQHVME GVGLGYFALK KIPVGDNTSY LHKVLSEVRI LEEVKQHPNM VEYKHSWVDT AQIADFGPPV RCLFILMEYA TIGSLDSYLE RHGSALPTIA VWYFFLSAVA GTAHLHRKNI IHRDLKPQNL LLTGRSEEVP RVLVSDFGTA TLLSEVSYER TGGTGTVEYM APELFECVPG TKESYIYSHT KATDVWSLGM ILHYLAFDGT LPLRLSNGEV VLEVENRSHY ARPPEMIQLM RLMLHRNPAK RPTCEELLES TVV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctaactc cacaatactt ccgtgcactt gctgcgactg ttactcgtcg gccgctttcc atatgtgaca gtccatcact tgaagctaaa ttccagcaac ggttggctga acagggtttg aagaaatatg tcccggaaga tgaaataaga ggaaaaatgg gagttacaga agaagaagaa gacacgtggg aactacccga tgaggcacat ggagcaacag cggtgttttc gtcggcgtta gatgaaaaca gcagaagggg cggcggacct gaaacgtctc ctccacgcgg tgataatacc agcggtgact gggggtatta taagcgttac tttagagaaa tacaaaaact aggaagcgga acctttggtg gagtgtatct gtgtcagcat gttatggaag gcgtggggtt ggggtacttt gctttaaaga aaattcccgt aggagacaat accagttact tgcacaaagt actcagtgag gtgcgtattc tcgaggaagt gaaacaacac ccaaacatgg tggagtataa gcatagctgg gtagataccg cgcaaatagc tgattttggg ccacccgtaa gatgcctttt tattcttatg gaatatgcca ctataggttc ccttgactcg taccttgagc ggcacggatc ggctctgcca acaatcgccg tatggtactt ctttttgagt gccgttgcgg ggacagcgca tttgcatcga aagaacatca tccatcggga cctcaagcca caaaatttac tgctgacagg tcggtcggag gaggtcccac gtgttcttgt tagcgacttt ggcactgcaa ctttactttc tgaggtgtca tatgaacgca cgggaggaac gggaaccgta gagtacatgg caccagagtt atttgagtgt gtgccgggca cgaaagagtc atatatatat tcgcacacaa aagcaacaga tgtgtggtca ctcggaatga tattacacta tcttgccttc gacggtacgc tgccgctgcg cttgtctaat ggtgaagttg tattggaagt ggagaaccgt tcccactacg ctcgccctcc agagatgatt caactaatgc gcctcatgct tcatcgtaac ccagcgaagc gacccacatg tgaggaattg ttagaatcga ctgtagtgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat