TrbrB.00424.a

Protein kinase, putative

CENTER ID: TrbrB.00424.a
ORGANISM: Trypanosoma brucei GUTat10.1
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrB.00424.a.B1.GE36986 full length 1 413
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrB.00424.a.B1.PS01867 full length 1 413

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:Tb927.10.12040
RefSeq: XP_823410.1
UniProt: Q389D8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNRSSRNLAE MQDEINQLDD RYKLEKIIGA GSYGVVIRAR DTTTNDLVAI KRVNKEIFEE IILAKRILRE IKLLAHFHHD NIIGLRNLIT PADRDNYDYF YIVMDVMETD LKQVLRTGQS LTEGHIQYFI YQILHALNFI HSSGVIHRDI TPANILVNKN CDLKICDFGL SKEESDQGEH MTDYVTMRWY RAPELVMEDK RYSAQIDVWG VGSILGELLG ARPLFQGKDR VNQLDKIIDV IGTPSEEDIN SIGSTAAQKY LKKKSFRPAP DWASVYPRAS PEALDLLRRM LVFHPDKRIT VAEALKHPFL RDLYEESDVQ VSIQHFNFDE TQHKTIQEVK WAVYDESVAF HKNHPRTAPR PQQQQHSAQQ PAAAASLPTV DIAGEWRSAQ QTIERGEEEC GAEQFDKYVG SVE
NT Sequence
atgaaccgta gttcgaggaa ccttgcagag atgcaggatg agatcaacca gttggacgac cgttacaaac ttgaaaagat aattggtgcg ggatcctatg gtgtcgtcat ccgcgcgagg gataccacaa caaacgacct agtggccatt aaacgcgtca ataaggaaat attcgaggaa ataatccttg caaaacgtat tcttcgcgaa atcaagctgt tggcacactt tcaccacgat aatatcattg gactccgtaa cctcatcacc ccggccgacc gcgacaacta tgattacttt tatattgtca tggatgtaat ggagaccgac ctgaagcaag tgctccgaac tggccaaagt ctcacggaag gtcacataca atatttcatt tatcaaatct tacatgccct aaacttcatc cattcttcag gtgttattca tcgagacatc accccggcca acattttagt caataaaaat tgtgatttga agatatgcga cttcggactt tccaaagagg agagtgatca aggggagcac atgacagatt atgttaccat gcgttggtac cgtgcaccgg agctggtgat ggaagataag aggtattccg cacaaattga tgtgtgggga gttggaagta ttcttggtga gttgctgggc gcccgacccc tgtttcaggg aaaagaccgt gtgaaccaac tggacaaaat cattgatgtt atcggaactc catctgaaga ggatataaat tccatcgggt ccactgcggc gcagaagtat ttaaaaaaga agtcatttcg tcctgcaccc gattgggcct ccgtatatcc cagagcgagt ccagaggcgc tggacttatt gcggcggatg ttggtgttcc atccagacaa gcgcataact gtcgctgaag cattaaaaca tcccttcctt agggatctgt acgaggaatc cgatgtacag gttagtatcc agcactttaa ctttgatgaa acacaacata aaacaattca ggaggtaaag tgggcggtgt atgatgagag tgtggcattt cacaaaaacc acccccggac agcacctagg ccacaacaac aacaacattc tgcgcaacaa cccgccgctg ctgcatctct tccaactgtt gatatcgctg gagagtggcg gtcagcgcaa caaacaattg agcggggcga ggaggaatgt ggtgcggagc agttcgacaa atatgtgggt tccgttgagt ga
Details for TrbrB.00424.a.B1.GE36986
HARVESTED ON: 5/21/2013
SEQUENCED ON: 5/23/2013
EXPECTED MW: 48kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 93
Validated AA Sequence
MQDEINQLDD RYKLEKIIGA GSYGVVIRAR DTTTNDLVAI KRVNKEIFEE IILAKRILRE IKLLAHFHHD NIIGLRNLIT PADRDNYDYF YIVMDVMETD LKQVLRTGQS LTEGHIQYFI YQILHALNFI HSSGVIHRDI TPANILVNKN CDLKICDFGL SKEESDQGEH MTDYVTMRWY RAPELVMEDK RYSAQIDVWG VGSILGELLG ARPLFQGKDR VNQLDKIIDV IGTPSEEDIN SIGSTAAQKY LKKKSFRPAP DWASVYPRAS PEALDLLRRM LVFHPDKRIT VAEALKHPFL RDLYEESDVQ VSIQHFNFDE TQHKTIQEVK WAVYDESVAF HKNHPRTAPR PQQXXHSAQQ PAAAASLPTV DIAGEWRSAQ QTIERGEEEC GA
Validated NT Sequence
ctccgcacca cattcctcct cgccccgctc aattgtttgt tgcgctgacc gccactctcc ggcgatatca acagttggaa gagatgcagc agcggcgggt tgttgcgcag aatgnnnnng ttgttgtggc ctaggtgctg tccgggggtg gtttttgtga aatgccacac tctcatcata caccgcccac tttacctcct gaattgtttt atgttgtgtt tcatcaaagt taaagtgctg gatactaacc tgtacatcgg attcctcgta cagatcccta aggaagggat gttttaatgc ttcagcgaca gttatgcgct tgtctggatg gaacaccaac atccgccgca ataagtccag cgcctctgga ctcgctctgg gatatacgga ggcccaatcg ggtgcaggac gaaatgactt cttttttaaa tacttctgcg ccgcagtgga cccgatggaa tttatatcct cttcagatgg agttccgata acatcaatga ttttgtccag ttggttcaca cggtcttttc cctgaaacag gggtcgggcg cccagcaact caccaagaat acttccaact ccccacacat caatttgtgc ggaatacctc ttatcttcca tcaccagctc cggtgcacgg taccaacgca tggtaacata atctgtcatg tgctcccctt gatcactctc ctctttggaa agtccgaagt cgcatatctt caaatcacaa tttttattga ctaaaatgtt ggccggggtg atgtctcgat gaataacacc tgaagaatgg atgaagttta gggcatgtaa gatttgataa atgaaatatt gtatgtgacc ttccgtgaga ctttggccag ttcggagcac ttgcttcagg tcggtctcca ttacatccat gacaatataa aagtaatcat agttgtcgcg gtcggccggg gtgatgaggt tacggagtcc aatgatatta tcgtggtgaa agtgtgccaa cagcttgatt tcgcgaagaa tacgttttgc aaggattatt tcctcgaata tttccttatt gacgcgttta atggccacta ggtcgtttgt tgtggtatcc ctcgcgcgga tgacgacacc ataggatccc gcaccaatta tcttttcaag tttgtaacgg tcgtccaact ggttgatctc atcctgcatc tctgcaaggt tcctcgaact a
Expected Protein Sequence
MAHHHHHHMN RSSRNLAEMQ DEINQLDDRY KLEKIIGAGS YGVVIRARDT TTNDLVAIKR VNKEIFEEII LAKRILREIK LLAHFHHDNI IGLRNLITPA DRDNYDYFYI VMDVMETDLK QVLRTGQSLT EGHIQYFIYQ ILHALNFIHS SGVIHRDITP ANILVNKNCD LKICDFGLSK EESDQGEHMT DYVTMRWYRA PELVMEDKRY SAQIDVWGVG SILGELLGAR PLFQGKDRVN QLDKIIDVIG TPSEEDINSI GSTAAQKYLK KKSFRPAPDW ASVYPRASPE ALDLLRRMLV FHPDKRITVA EALKHPFLRD LYEESDVQVS IQHFNFDETQ HKTIQEVKWA VYDESVAFHK NHPRTAPRPQ QQQHSAQQPA AAASLPTVDI AGEWRSAQQT IERGEEECGA EQFDKYVGSV E
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc gtagttcgag gaaccttgca gagatgcagg atgagatcaa ccagttggac gaccgttaca aacttgaaaa gataattggt gcgggatcct atggtgtcgt catccgcgcg agggatacca caacaaacga cctagtggcc attaaacgcg tcaataagga aatattcgag gaaataatcc ttgcaaaacg tattcttcgc gaaatcaagc tgttggcaca ctttcaccac gataatatca ttggactccg taacctcatc accccggccg accgcgacaa ctatgattac ttttatattg tcatggatgt aatggagacc gacctgaagc aagtgctccg aactggccaa agtctcacgg aaggtcacat acaatatttc atttatcaaa tcttacatgc cctaaacttc atccattctt caggtgttat tcatcgagac atcaccccgg ccaacatttt agtcaataaa aattgtgatt tgaagatatg cgacttcgga ctttccaaag aggagagtga tcaaggggag cacatgacag attatgttac catgcgttgg taccgtgcac cggagctggt gatggaagat aagaggtatt ccgcacaaat tgatgtgtgg ggagttggaa gtattcttgg tgagttgctg ggcgcccgac ccctgtttca gggaaaagac cgtgtgaacc aactggacaa aatcattgat gttatcggaa ctccatctga agaggatata aattccatcg ggtccactgc ggcgcagaag tatttaaaaa agaagtcatt tcgtcctgca cccgattggg cctccgtata tcccagagcg agtccagagg cgctggactt attgcggcgg atgttggtgt tccatccaga caagcgcata actgtcgctg aagcattaaa acatcccttc cttagggatc tgtacgagga atccgatgta caggttagta tccagcactt taactttgat gaaacacaac ataaaacaat tcaggaggta aagtgggcgg tgtatgatga gagtgtggca tttcacaaaa accacccccg gacagcacct aggccacaac aacaacaaca ttctgcgcaa caacccgccg ctgctgcatc tcttccaact gttgatatcg ctggagagtg gcggtcagcg caacaaacaa ttgagcgggg cgaggaggaa tgtggtgcgg agcagttcga caaatatgtg ggttccgttg agtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for TrbrB.00424.a.B1.PS01867
PURIFICATION DATe: 7/18/2013
CONCENTRATION: 37.3mg/ml
OBSERVED MW: 48kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 17
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MQDEINQLDD RYKLEKIIGA GSYGVVIRAR DTTTNDLVAI KRVNKEIFEE IILAKRILRE IKLLAHFHHD NIIGLRNLIT PADRDNYDYF YIVMDVMETD LKQVLRTGQS LTEGHIQYFI YQILHALNFI HSSGVIHRDI TPANILVNKN CDLKICDFGL SKEESDQGEH MTDYVTMRWY RAPELVMEDK RYSAQIDVWG VGSILGELLG ARPLFQGKDR VNQLDKIIDV IGTPSEEDIN SIGSTAAQKY LKKKSFRPAP DWASVYPRAS PEALDLLRRM LVFHPDKRIT VAEALKHPFL RDLYEESDVQ VSIQHFNFDE TQHKTIQEVK WAVYDESVAF HKNHPRTAPR PQQXXHSAQQ PAAAASLPTV DIAGEWRSAQ QTIERGEEEC GA
Validated NT Sequence
ctccgcacca cattcctcct cgccccgctc aattgtttgt tgcgctgacc gccactctcc ggcgatatca acagttggaa gagatgcagc agcggcgggt tgttgcgcag aatgnnnnng ttgttgtggc ctaggtgctg tccgggggtg gtttttgtga aatgccacac tctcatcata caccgcccac tttacctcct gaattgtttt atgttgtgtt tcatcaaagt taaagtgctg gatactaacc tgtacatcgg attcctcgta cagatcccta aggaagggat gttttaatgc ttcagcgaca gttatgcgct tgtctggatg gaacaccaac atccgccgca ataagtccag cgcctctgga ctcgctctgg gatatacgga ggcccaatcg ggtgcaggac gaaatgactt cttttttaaa tacttctgcg ccgcagtgga cccgatggaa tttatatcct cttcagatgg agttccgata acatcaatga ttttgtccag ttggttcaca cggtcttttc cctgaaacag gggtcgggcg cccagcaact caccaagaat acttccaact ccccacacat caatttgtgc ggaatacctc ttatcttcca tcaccagctc cggtgcacgg taccaacgca tggtaacata atctgtcatg tgctcccctt gatcactctc ctctttggaa agtccgaagt cgcatatctt caaatcacaa tttttattga ctaaaatgtt ggccggggtg atgtctcgat gaataacacc tgaagaatgg atgaagttta gggcatgtaa gatttgataa atgaaatatt gtatgtgacc ttccgtgaga ctttggccag ttcggagcac ttgcttcagg tcggtctcca ttacatccat gacaatataa aagtaatcat agttgtcgcg gtcggccggg gtgatgaggt tacggagtcc aatgatatta tcgtggtgaa agtgtgccaa cagcttgatt tcgcgaagaa tacgttttgc aaggattatt tcctcgaata tttccttatt gacgcgttta atggccacta ggtcgtttgt tgtggtatcc ctcgcgcgga tgacgacacc ataggatccc gcaccaatta tcttttcaag tttgtaacgg tcgtccaact ggttgatctc atcctgcatc tctgcaaggt tcctcgaact a
Expressed Protein Sequence
MAHHHHHHMN RSSRNLAEMQ DEINQLDDRY KLEKIIGAGS YGVVIRARDT TTNDLVAIKR VNKEIFEEII LAKRILREIK LLAHFHHDNI IGLRNLITPA DRDNYDYFYI VMDVMETDLK QVLRTGQSLT EGHIQYFIYQ ILHALNFIHS SGVIHRDITP ANILVNKNCD LKICDFGLSK EESDQGEHMT DYVTMRWYRA PELVMEDKRY SAQIDVWGVG SILGELLGAR PLFQGKDRVN QLDKIIDVIG TPSEEDINSI GSTAAQKYLK KKSFRPAPDW ASVYPRASPE ALDLLRRMLV FHPDKRITVA EALKHPFLRD LYEESDVQVS IQHFNFDETQ HKTIQEVKWA VYDESVAFHK NHPRTAPRPQ QQQHSAQQPA AAASLPTVDI AGEWRSAQQT IERGEEECGA EQFDKYVGSV E
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACC GTAGTTCGAG GAACCTTGCA GAGATGCAGG ATGAGATCAA CCAGTTGGAC GACCGTTACA AACTTGAAAA GATAATTGGT GCGGGATCCT ATGGTGTCGT CATCCGCGCG AGGGATACCA CAACAAACGA CCTAGTGGCC ATTAAACGCG TCAATAAGGA AATATTCGAG GAAATAATCC TTGCAAAACG TATTCTTCGC GAAATCAAGC TGTTGGCACA CTTTCACCAC GATAATATCA TTGGACTCCG TAACCTCATC ACCCCGGCCG ACCGCGACAA CTATGATTAC TTTTATATTG TCATGGATGT AATGGAGACC GACCTGAAGC AAGTGCTCCG AACTGGCCAA AGTCTCACGG AAGGTCACAT ACAATATTTC ATTTATCAAA TCTTACATGC CCTAAACTTC ATCCATTCTT CAGGTGTTAT TCATCGAGAC ATCACCCCGG CCAACATTTT AGTCAATAAA AATTGTGATT TGAAGATATG CGACTTCGGA CTTTCCAAAG AGGAGAGTGA TCAAGGGGAG CACATGACAG ATTATGTTAC CATGCGTTGG TACCGTGCAC CGGAGCTGGT GATGGAAGAT AAGAGGTATT CCGCACAAAT TGATGTGTGG GGAGTTGGAA GTATTCTTGG TGAGTTGCTG GGCGCCCGAC CCCTGTTTCA GGGAAAAGAC CGTGTGAACC AACTGGACAA AATCATTGAT GTTATCGGAA CTCCATCTGA AGAGGATATA AATTCCATCG GGTCCACTGC GGCGCAGAAG TATTTAAAAA AGAAGTCATT TCGTCCTGCA CCCGATTGGG CCTCCGTATA TCCCAGAGCG AGTCCAGAGG CGCTGGACTT ATTGCGGCGG ATGTTGGTGT TCCATCCAGA CAAGCGCATA ACTGTCGCTG AAGCATTAAA ACATCCCTTC CTTAGGGATC TGTACGAGGA ATCCGATGTA CAGGTTAGTA TCCAGCACTT TAACTTTGAT GAAACACAAC ATAAAACAAT TCAGGAGGTA AAGTGGGCGG TGTATGATGA GAGTGTGGCA TTTCACAAAA ACCACCCCCG GACAGCACCT AGGCCACAAC AACAACAACA TTCTGCGCAA CAACCCGCCG CTGCTGCATC TCTTCCAACT GTTGATATCG CTGGAGAGTG GCGGTCAGCG CAACAAACAA TTGAGCGGGG CGAGGAGGAA TGTGGTGCGG AGCAGTTCGA CAAATATGTG GGTTCCGTTG AGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT