TrbrA.20384.a

"Protein kinase, putative (EC 2.7.1.-)"

CENTER ID: TrbrA.20384.a
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.20384.a.HZ2.GE43761 kinase domain 1 372
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:Tb10.70.5760
UniProt: Q38C39

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRANDIVKGR YSTYQLQDLI GKGGNAVVYS AVDRNTGLMV AVKKMEVHDD AAMATCRNEV AILSHLKHPY VIRYIDHAHS GKSFLIVTEF ALERSLLQRL KRHGRCGELE VARFMFQITS ALAYLHKEKI VHRDIKCANV LLGAAEVVKL SDFGLAVNLG AVHGDTNDEE DGLLSSSAVC DGDGKSVAEE GIVGSVYWMA PEAVRGEPPA ESCDIWSLGC LCIELLTGNP PFFDRKPANA LYCISETDET PIPTMDVSEE CRNFLTQCLD RDASKRPTAA ALLKHGWFGG FLAENIVKTL ADTQVDGTTS DQVASNSEAI KSWVENNLFN ERAEQREEWL RSGCLKRVVA VLPKVTPKVS FHIIRTFVFA AQRCRTESSC FLTRLGEADL WDSAQLGVLG KAESLGALFV CCCERQDPRV LQYAPTHPGA LWFLLQCAEV DVAIMCIDAL RALLVGTAGE EEENRASFSS RPEVASTNVE NITFRRYVSQ NRFVSDHAVY AVQRIIEDIC YDAFRGDKDP AVGWQNIDKL FEILLQVYRK AGDESIILGP SVSGARQDDP QDSPKGGTGG SVAATATAAV AVSTSIVGGG GGAASAMGDP AGSAATATTS APSSGEAGRK PINPHIHSPC SPNAGGVNTS SVVVGIASAC GKGGKPAEEL WLLALQEASR KLCPNAIELL MQYVHLAARN DLKSLNIGGK AIAGTFLVVA SNASVDTATR ITVLKCLPAL QCASPKAANF MRDVRCSVPL LVHTAKNIPV PAEARDDEVL KVLFALCEDN RVAAAFASYG AFIAIAIDRA TLACERQRWA EVTLAVRFVE LLLAHATDTS CILESHALLR LLLKLSDGDK FPSIVTSVAQ RLLNSLQAQT NVG
NT Sequence
atgcgggcta acgacattgt gaagggtaga tattccacat atcagttaca ggaccttatt ggtaagggtg gtaatgctgt tgtgtacagc gccgtcgacc gcaacaccgg gctcatggtt gctgtcaaga aaatggaggt tcatgatgat gcagctatgg ctacgtgccg gaatgaagtg gccatactta gccacttgaa gcatccttat gtcatccgct acattgatca tgcacactct ggcaagtctt ttctcattgt taccgaattt gcgcttgaac gttccctatt gcagagactg aagcgtcacg gccggtgtgg tgagctggag gtggcccgtt tcatgtttca gataacttct gcgttggctt acttgcacaa agagaagatc gtgcaccgtg atataaaatg cgccaacgta ctcttgggtg cagctgaagt tgttaagtta tcggattttg gtttggctgt gaaccttggt gccgtacacg gagacacaaa tgatgaagag gatggcttgc tgtcaagcag cgcagtgtgt gatggggatg gtaaatctgt cgcagaagaa ggtatagtgg gttctgtgta ttggatggct cccgaagctg tgcggggtga gccacctgca gagagttgcg acatttggtc gcttgggtgc ctttgcattg aactattgac aggtaaccca cccttctttg atcgtaaacc tgccaacgca ttgtattgta taagcgaaac ggatgaaaca ccaataccta ccatggatgt gtcggaggag tgccgcaatt tcttgacaca gtgtcttgac agggacgcgt ctaagcggcc gaccgcagcg gcgttgttga agcacggatg gtttggtggc ttccttgcgg agaacattgt aaaaacactc gcagacactc aggttgatgg cactacctct gaccaggtgg ctagtaattc cgaagcgatt aagagttggg tggagaataa cctctttaac gagcgtgcgg agcaacgtga ggaatggctc aggagtgggt gcctgaagag agtcgttgca gtactaccga aggtgacacc aaaggtttct tttcacataa ttcgcacctt tgtttttgcc gcacagcgtt gtcgtacgga gtcaagctgt tttctaaccc ggctcggcga agccgatctg tgggacagtg cgcagcttgg agttcttggg aaggccgaat cacttggcgc gctttttgtt tgttgctgtg agcgacaaga cccacgcgtg ctgcagtatg ctcctactca cccgggggca ctctggttcc ttttgcagtg tgcggaggtt gatgttgcga tcatgtgcat tgacgctctg cgcgccctgc tcgtaggaac ggccggtgag gaggaagaaa accgagcttc cttttcgtca cgccccgagg ttgcttccac aaacgtggag aatataacgt ttcgtcgcta cgtctctcag aaccgtttcg tttctgacca cgccgtttac gctgttcagc gcattataga ggacatatgc tacgacgcat tccgtggtga caaggatccc gctgtcgggt ggcagaatat cgacaaactt tttgaaatac ttttacaggt gtacaggaag gcaggagacg agagtattat actaggcccg agtgtgagtg gtgcacggca ggacgaccct caagattcac ccaagggggg cacaggtggt tctgtggctg ccaccgcgac agctgctgtt gctgtatcga cttccatagt agggggaggg ggtggagcag catcagctat gggagatccc gctggaagcg cggccacggc cacaactagt gcgccctcat caggtgaagc aggcaggaaa cccatcaatc ctcatatcca ctccccctgc agccccaacg ccggtggagt taacaccagc agcgtcgttg ttggcattgc cagtgcctgc ggtaaaggtg gaaaaccagc ggaggagctg tggttgttag cgttgcagga ggcgagccga aaactctgtc cgaatgccat tgaacttctt atgcagtacg ttcatcttgc tgctcgcaat gatctcaaat cccttaacat cggcggtaaa gccattgcag gcacgtttct tgtcgttgct tccaacgctt ctgttgacac ggccacgcgc attacagtgc tcaagtgcct ccccgcctta caatgtgcat ccccaaaggc agccaacttt atgagagacg tgcgatgtag tgttccactc ttagtgcaca cggcaaagaa tatcccggtg ccagcggagg cgcgagacga tgaggtgttg aaggtccttt tcgctctctg cgaggacaac agggttgctg cagccttcgc ctcttacggt gcttttattg cgatcgcaat tgaccgcgct acgcttgcat gtgagcgaca gcgctgggcg gaagtcactt tagctgtgcg gtttgtagag cttttgctcg cgcacgccac agacacctcg tgcatactag agtcccatgc tttgctgcgt ttgctgctga agctttccga tggagacaag tttccatcga tagtaacatc tgtggcgcag cgattattaa acagtttgca ggcacagaca aatgtgggat ga
Details for TrbrA.20384.a.HZ2.GE43761
HARVESTED ON: data unavailable
SEQUENCED ON: 6/23/2020
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMR ANDIVKGRYS TYQLQDLIGK GGNAVVYSAV DCNTGLMVAV KKMEVHDDAA MATCRNEVAI LSHLKHPYVI RYIDHAHSGK SFLIVTEFAL ERSLLQRLKR HGRCGELEVA RFMFQITSAL AYLHKEKIVH RDIKCANVLL GAAEVVKLSD FGLAVNLGAV HGDTNDEEDG LLSSSAVCDG DGKSVAEEGI VGSVYWMAPE AVRGEPPAES CDIWSLGCLC IELLTGNPPF FDRKPANALY CISETDETPI PTMDVSEECR NFLTQCLDRD ASKRPTAAAL LKHGWFGGFL AENIVKTLAD TQVDGTTSDQ VASNSEAIKS WVENNLFNER AEQREEWLRS GCLKRVVAVL PKVTPKVSFH IIRTFVFAA
Validated NT Sequence
ctgtgcggca aaaacaaagg tgcgaattat gtgaaaagaa acctttggtg tcaccttcgg tagtactgca acgactctct tcaggcaccc actcctgagc cattcctcac gttgctccgc acgctcgtta aaaaggttat tctccaccca actcttaatc gcttcggaat tactagccac ctggtcagag gtagtgccat caacctgagt gtctgcgagt gtttttacaa tgttctccgc aaggaagcca ccaaaccatc cgtgcttcaa caacgccgct gcggtcggcc gcttagacgc gtccctgtca agacactgcg tcaagaaatt gcggcactcc tccgacacat ccatggtagg tattggtgtt tcatccgttt cgcttataca atacaatgcg ttggcaggtt tacgatcaaa gaagggtggg ttacctgtca atagttcaat gcaaaggcac ccaagcgacc aaatgtcgca actctctgca ggtggctcac cccgcacagc ttcgggagcc atccaataca cagaacccac tataccttct tctgcgacag atttaccatc cccatcacac actgcgctgc ttgacagcaa gccatcctct tcatcatttg tgtctccgtg tacggcacca aggttcacag ccaaaccaaa atccgataac ttaacaactt cagctgcgcc caagagtacg ttggcgcatt ttatatcacg gtgcacgatc ttctctttgt gcaagtaagc caacgcagaa gttatctgaa acatgaaacg ggccacctcc agctcaccac accggccgtg acgcttcagt ctctgcaata gggaacgttc aagcgcaaat tcggtaacaa tgagaaaaga cttgccagag tgtgcatgat caatgtagcg gatgacataa ggatgcttca agtggctaag tatggccact tcattccggc acgtagccat agctgcatca tcatgaacct ccattttctt gacagcaacc atgagcccgg tgttgcagtc gacggcgctg tacacaacag cattaccacc cttaccaata aggtcctgca actgatatgt ggaatatcta cccttcacaa tgtcgttagc ccgcatcgaa ccaggaccct gggtctgagc ttccagggta ccaccaatct gttctctgtg agcctcaata atatcgttat cctccatgtc caaatcttca ggggtctgat cagcttgaat tctaataccg tcgtacaaga atcttaagga gtccatttcc ttaccctgtc ttttagcgaa cgcttccatc agccttctta aaggagtggt ctttttgatc ttgaagaaga tctctgaaga tccatcggac acctttaaat tgatgtgagt ctcaggcttg acttctggct tgacctctgg cttagcttct tgattgactt ctgagtccga cttcatatga tggtgatggt gatgagccat
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMR ANDIVKGRYS TYQLQDLIGK GGNAVVYSAV DRNTGLMVAV KKMEVHDDAA MATCRNEVAI LSHLKHPYVI RYIDHAHSGK SFLIVTEFAL ERSLLQRLKR HGRCGELEVA RFMFQITSAL AYLHKEKIVH RDIKCANVLL GAAEVVKLSD FGLAVNLGAV HGDTNDEEDG LLSSSAVCDG DGKSVAEEGI VGSVYWMAPE AVRGEPPAES CDIWSLGCLC IELLTGNPPF FDRKPANALY CISETDETPI PTMDVSEECR NFLTQCLDRD ASKRPTAAAL LKHGWFGGFL AENIVKTLAD TQVDGTTSDQ VASNSEAIKS WVENNLFNER AEQREEWLRS GCLKRVVAVL PKVTPKVSFH IIRTFVFAAQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga tgcgggctaa cgacattgtg aagggtagat attccacata tcagttacag gaccttattg gtaagggtgg taatgctgtt gtgtacagcg ccgtcgaccg caacaccggg ctcatggttg ctgtcaagaa aatggaggtt catgatgatg cagctatggc tacgtgccgg aatgaagtgg ccatacttag ccacttgaag catccttatg tcatccgcta cattgatcat gcacactctg gcaagtcttt tctcattgtt accgaatttg cgcttgaacg ttccctattg cagagactga agcgtcacgg ccggtgtggt gagctggagg tggcccgttt catgtttcag ataacttctg cgttggctta cttgcacaaa gagaagatcg tgcaccgtga tataaaatgc gccaacgtac tcttgggtgc agctgaagtt gttaagttat cggattttgg tttggctgtg aaccttggtg ccgtacacgg agacacaaat gatgaagagg atggcttgct gtcaagcagc gcagtgtgtg atggggatgg taaatctgtc gcagaagaag gtatagtggg ttctgtgtat tggatggctc ccgaagctgt gcggggtgag ccacctgcag agagttgcga catttggtcg cttgggtgcc tttgcattga actattgaca ggtaacccac ccttctttga tcgtaaacct gccaacgcat tgtattgtat aagcgaaacg gatgaaacac caatacctac catggatgtg tcggaggagt gccgcaattt cttgacacag tgtcttgaca gggacgcgtc taagcggccg accgcagcgg cgttgttgaa gcacggatgg tttggtggct tccttgcgga gaacattgta aaaacactcg cagacactca ggttgatggc actacctctg accaggtggc tagtaattcc gaagcgatta agagttgggt ggagaataac ctctttaacg agcgtgcgga gcaacgtgag gaatggctca ggagtgggtg cctgaagaga gtcgttgcag tactaccgaa ggtgacacca aaggtttctt ttcacataat tcgcaccttt gtttttgccg cacagtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa