TrbrA.20383.a

"Protein kinase, putative (EC 2.7.1.-)"

CENTER ID: TrbrA.20383.a
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.20383.a.HZ2.GE43760 kinase domain 1 360
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:Tb11.02.2050
UniProt: Q385X0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPSNSVVGHL SLWLCRCCFP KIRNMDNYTI VELIGEGSFG KVYKARRKGT GHIVAMKFIV KKGKNDKELL NLRSEIEIMT KLNHDNIITL FEAFETQQEF VVVMEYAQGE LFEILEDDKK LPEEVVRRIA KQLLQALHYL HSNRIMHRDM KPQNILIGQN GSVKLADFGF ARTMSYNTMV LTSIKGTPLY MAPELVQEQP YNHTADLWSL GCILYELLYG KPPFYTNHLY KLINQIVNDP VRFEEPISPD FKSLLKGLLT KSFSARLNWP HLLNHPFVAI TGDDEKWLMA VKQHDTKMKE RMERLECLRH HAQPNRVVEL DAGGPCQTPG NTHGFPLGSE EDEIFSPSSL KCLLSPSDGE GCIEAFRGLL VAVENATSLP LQKTALLERI LHVGVLGPIV RHLCDKQSAE MSRLAVQIIK TLVFPEGNAV LPFPSQRPLQ ETIDASPQPG DLPPVGLLIR QQVSLELLEK PRESLDFLVK EVIENRHGFR VDCVKIIFQC VRWGVGFGPV LTQLKLFPSF WASLLDSVTE GSHFQTYAAL AFHTVSAMIP HIKLSAPDQI NADKVSAFVS IGLLAVRFYD LSKSSDIAAL NSAAAAALLA AFVHREMKDT MVFEPDAAFM EGLCAIVKDV RGIADRNSVW RILGNGYGYP DYGFLDGVVH TLSVMFSNPQ SIVYQNSGQL PSRSYLDSDQ KGLLRIAVEL LRDSDPKTEL SPNGVVAALR CVQQACQHQR RDEHFTSLLL ERIEAYRGDS GGPVSVAGVV CHQLRASYLG QLRSWPEHSG GGTVGVNAHL TVVVQILLAA LQTSKQGSES DDEANASVQQ IFYKEGLMEM LIVALDYTQV AFWGPPFNII TKLVVGSPPF AKAFVDGGGL QCERIGKVLD SKKASAGLVS DGLNVLSQLA RLSKDFYLPI HNADLYSAVL ELLRHSDPGI RSKSCNLVGN LCKHSPYLYE PLARHGIIDM LVERCSDRDP ATQKFAAFAV GNAAFHNGSL YEKLRPSIPV MVKLLTSSDE KTRQNAAGAV SNFVRNGSTL TNALMENSAI ETLSHMLKKD KVPLRKIALI TIGSFCAYEN CKAKFLALGL EGTIRQLEES GICNVDPSIT KYVARIRQRI SSS
NT Sequence
atgccatcta attctgttgt aggtcacctt tctttgtggt tgtgtaggtg ctgctttccg aaaattcgga atatggacaa ctataccata gtcgaactta taggggaagg gtcgtttgga aaagtttaca aggcgagaag aaagggtacg ggtcacatcg tggcgatgaa gttcatagtc aagaaaggta aaaatgacaa agaacttctt aaccttcgta gtgaaattga aattatgact aaactcaacc atgacaacat cataacgttg tttgaggctt ttgaaactca acaagaattc gttgtggtaa tggaatacgc ccaaggtgaa ttatttgaaa ttcttgaaga cgataaaaaa ctccccgaag aggttgtccg gcgcatcgcg aagcaattgt tgcaagcact tcattacttg cactcgaacc gcattatgca ccgcgacatg aaacctcaga acatcctcat aggacagaac ggatccgtga aacttgcgga tttcggattt gcgaggacga tgagctacaa tacgatggtg ctaaccagta ttaagggaac acccctctat atggcaccag aacttgtgca agagcagcca tacaaccaca cagcagattt gtggtctctc gggtgcattc tatatgagtt gttgtacggc aagccgccct tttatacgaa ccatctttat aagctgatca accaaatcgt caacgacccc gtaagatttg aagaacctat atcaccggac ttcaagtcgc ttttgaaagg acttttgacc aaatcgttct cggccagatt aaactggcca catttactta accacccatt tgttgccatc actggggacg atgagaagtg gttgatggcg gtaaaacagc acgacacgaa gatgaaggaa cgcatggaac gactagagtg cctgcgacat cacgctcaac caaacagggt tgttgaatta gatgcaggtg gcccgtgcca aacgcccggg aacacgcatg ggtttccact gggttcggaa gaagatgaaa tattttcacc ttcctcgtta aagtgtctac tttctccttc agatggcgaa ggttgtattg aggcgttccg tgggctgcta gttgcggttg aaaacgctac atcattgccc cttcagaaaa ctgctttgtt agaacgaatc ctacacgttg gtgttttggg acctatagta cggcacttat gtgacaagca gtcagccgaa atgagccgac ttgctgtcca gataatcaaa accctcgtgt ttccggaggg taatgctgtg ttaccgtttc cttctcagcg acccttacaa gaaacaatag atgcatcacc gcagccaggc gacttgccac ctgttggcct tcttattcga caacaggttt ctctggagct actagaaaaa ccccgagagt ccctcgactt tcttgtgaaa gaagtaattg aaaatcgtca tgggtttcgc gtggattgtg ttaaaataat tttccagtgt gtgaggtggg gagtaggttt tggacctgtt ttgacccagt tgaagctttt cccttcattt tgggcgtcat tacttgactc cgtgacagag ggaagccatt ttcagacgta cgcggcacta gcattccaca ccgtttctgc gatgattccg cacataaagt tgtccgcccc agatcagata aatgcagaca aggtgtccgc atttgtctct attggtttgc tagccgtgcg tttctatgat ttgtcaaaaa gttcggatat agctgcgctt aactctgccg ccgctgcagc tcttctggcc gcatttgttc accgggaaat gaaggacaca atggttttcg aaccagacgc ggcgttcatg gaagggttat gtgccatagt gaaagatgta agaggaattg ccgatcgcaa tagtgtctgg cgaattcttg gaaatggcta tggctacccg gattacggat ttttggatgg agtagtacat actttgtcgg tgatgttttc taatccacaa tctatcgtat accaaaatag tggtcagctc ccttcgcgct cataccttga cagcgatcaa aaaggactgt tgcgcattgc tgttgaacta ctccgcgata gtgacccaaa aaccgaactg tcgcctaatg gtgtggttgc tgctcttcga tgcgtgcagc aggcctgcca gcatcaacga agggatgagc attttactag tcttctgttg gaacggatag aagcatatcg tggggacagt ggaggtccag tttcggttgc cggcgttgtt tgtcatcaac tacgagcaag ctatctaggg caacttcgct cttggcccga acatagtggt ggtggcacgg ttggagtaaa cgcgcatctt accgtagttg ttcagatact gctagcggca cttcagacat ccaaacaggg ttcggagtca gacgacgaag ccaacgcctc tgtgcagcaa attttttaca aagagggact tatggagatg ctgattgttg cgcttgacta cactcaggta gcattttggg gtccaccgtt caacattata acaaagctgg tggtagggtc accgccattc gccaaggctt tcgttgacgg tggtggactg cagtgtgaga gaataggaaa ggtcctggat tcgaagaagg caagcgcagg gctcgtgtcg gacggactta atgttctctc tcaactagcc cggctttcca aagattttta cctgccaatt cataatgcag acttgtacag cgcggtttta gaactgctgc ggcacagtga cccagggatt cggagcaaat catgtaacct ggtaggaaac ctatgtaaac actcaccata cttgtacgaa cccctcgcga gacacggcat aattgatatg ctggttgaga gatgtagtga cagggatccc gcaacgcaga aatttgcagc atttgctgtt gggaatgctg cgttccacaa tggctccctt tacgagaagc taaggccttc gattcccgtc atggtgaagc ttttgactag tagcgatgag aaaacgaggc agaacgctgc tggagccgta agtaacttcg tacgaaatgg aagcacactt acaaacgcac taatggaaaa cagtgctatc gaaactcttt cccacatgct caagaaagat aaggtgcccc tccgaaaaat tgcacttatc accataggtt ccttctgcgc ttatgaaaat tgcaaagcga agttcctggc actaggtcta gagggaacta ttaggcaact tgaagagagt ggcatctgta atgtcgatcc ctccatcaca aagtacgtgg cacggataag gcaaaggatt tcgtcctcgt ga
Details for TrbrA.20383.a.HZ2.GE43760
HARVESTED ON: data unavailable
SEQUENCED ON: 6/23/2020
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 90
PERCENT COVERAGE: 57
Validated AA Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMP SNSVVGHLSL WLCRCCFPKI RNMDNYTXVE LIGEXSFGKV YKXRRKGTGH IVAMKFIVKK GKNDKELXNL RSEIEIMTKL NHDNIITLFE AFETQQEFVV VMEYAQGELF EIXEDXKKLP EXXXRRIAKQ LLQSTSXLAL EPHYAPRHET SXXSS
Validated NT Sequence
tattcgccat ctgaaggaga aagtagacac tttaacgagg aaggtgaaaa tatttcatct tcttccgaac ccagtggaaa cccatgcgtg ttcccgggcg tttggcacgg gccacctgca tctaattcaa caaccctgtt tggttgagca tgatgtcgta ggcactctag tcgttccatg cgttccttca tcttcgtgtc gtgctgtttt accgccatca accacttctc atcgtcccca gtgatggcaa caaatgggtg gttaagtaaa tgtggccagt ttaatctggc cgagaacgat ttggtcagaa gtcctttcaa aagcgacttg aagtccggng atataggttc ttcaaatctt acggggtcgt tgacgatttg gttgatcagc ttataaagat ggttcgtata aaagggcggc ttgccgcaca acaactcata tagaatgcac ccgagagacc acaaatctgc tgtgtggttg tatggctgct cttgcacaag ttctggtgcc atatagaggg gtgttccctt aatactggtt agcaccatcg tattgtanct catcnncctc ncaaatccga aatccncnng tttcacngat ccgttctgtc ctatgangat gntnntgang tttcatgtcg cggtgcataa tgcggttcga gtgcaagnaa tgaagtgctt tgcaacaatt gcttcgcgat gcgccggann nnntcttcgg ggagtttttt nncgtcttca anaatttcaa ataattcacc ttgggcgtat tccattacca caacgaattc ttgttgagtt tcaaaagcct caaacaacgt tatgatgttg tcatggttga gtttagtcat aatttcaatt tcactacgaa ggttangaag ttctttgtca tttttncctt tcttgactat gaacttcatc gccacgatgt gacccgtacc ctttcttctc nccttgtaaa cttttccaaa cgaccnttcc cctataagtt cgactanggt atagttgtcc atattccgaa ttttcggaaa gcagcaccta cacaaccaca aagaaaggtg acctacaaca gaattagatg gcatcgaacc aggaccctgg gtctgagctt ccagggtacc accaatctgt tctctgtgag cctcaataat atcgttatcc tccatgtcca aatcttcagg ggtctgatca gcttgaattc taataccgtc gtacaagaat cttaaggagt ccatttcctt accctgtctt ttagcgaacg cttccatcag ccttcttaaa ggagtggtct ttttgatctt gaagaagatc tctgaagatc catcggacac ctttaaattg atgtgagtct caggcttgac ttctggcttg acctctggct tagcttcttg attgacttct gagtccgact tcatatgatg gtgatggtga tgagccat
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMP SNSVVGHLSL WLCRCCFPKI RNMDNYTIVE LIGEGSFGKV YKARRKGTGH IVAMKFIVKK GKNDKELLNL RSEIEIMTKL NHDNIITLFE AFETQQEFVV VMEYAQGELF EILEDDKKLP EEVVRRIAKQ LLQALHYLHS NRIMHRDMKP QNILIGQNGS VKLADFGFAR TMSYNTMVLT SIKGTPLYMA PELVQEQPYN HTADLWSLGC ILYELLYGKP PFYTNHLYKL INQIVNDPVR FEEPISPDFK SLLKGLLTKS FSARLNWPHL LNHPFVAITG DDEKWLMAVK QHDTKMKERM ERLECLRHHA QPNRVVELDA GGPCQTPGNT HGFPLGSEED EIFSPSSLKC LLSPSDGE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga tgccatctaa ttctgttgta ggtcaccttt ctttgtggtt gtgtaggtgc tgctttccga aaattcggaa tatggacaac tataccatag tcgaacttat aggggaaggg tcgtttggaa aagtttacaa ggcgagaaga aagggtacgg gtcacatcgt ggcgatgaag ttcatagtca agaaaggtaa aaatgacaaa gaacttctta accttcgtag tgaaattgaa attatgacta aactcaacca tgacaacatc ataacgttgt ttgaggcttt tgaaactcaa caagaattcg ttgtggtaat ggaatacgcc caaggtgaat tatttgaaat tcttgaagac gataaaaaac tccccgaaga ggttgtccgg cgcatcgcga agcaattgtt gcaagcactt cattacttgc actcgaaccg cattatgcac cgcgacatga aacctcagaa catcctcata ggacagaacg gatccgtgaa acttgcggat ttcggatttg cgaggacgat gagctacaat acgatggtgc taaccagtat taagggaaca cccctctata tggcaccaga acttgtgcaa gagcagccat acaaccacac agcagatttg tggtctctcg ggtgcattct atatgagttg ttgtacggca agccgccctt ttatacgaac catctttata agctgatcaa ccaaatcgtc aacgaccccg taagatttga agaacctata tcaccggact tcaagtcgct tttgaaagga cttttgacca aatcgttctc ggccagatta aactggccac atttacttaa ccacccattt gttgccatca ctggggacga tgagaagtgg ttgatggcgg taaaacagca cgacacgaag atgaaggaac gcatggaacg actagagtgc ctgcgacatc acgctcaacc aaacagggtt gttgaattag atgcaggtgg cccgtgccaa acgcccggga acacgcatgg gtttccactg ggttcggaag aagatgaaat attttcacct tcctcgttaa agtgtctact ttctccttca gatggcgaat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa