TrbrA.20382.a

Serine/arginine-rich protein specific kinase SRPK, putative

CENTER ID: TrbrA.20382.a
ORGANISM: Trypanosoma brucei TREU927
ASSOCIATED DISEASE: African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrbrA.20382.a.HZ2.GE43759 kinase domain 130 584
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:Tb927.6.4970
UniProt: Q586G9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPRPDRRAKY GRRAQNKITT GGGGAKRKSK PIAPHVEKDG IDINALLEEI ERNDSPAEEK VPKETTKKSR RRRKAEKVEG LAVKPGTTVA QARMLASKMQ QRHSASTNGP SQPAHQRSEE DEDEDDDDEE QDGSGEDYSD IANERPSEYR KGGYHPVVVG EVYNQRYRVV RKLGWGYFST VWLVWDYVEK VFQAMKVQKS AKHYTEAAYD EIKLLGEIMT ADPEKVRRCA RLNDHFEQQG PNGKHVCMVF DVYGEDLLSL IERYKYHGVP LPIVKCISRQ ILIGLEHVHS LDIIHTDLKP ENVLLSAPKH AIVSQMKRFK PPPLHDRPSL VKRDPKTMTK SQRRRYYKKL RAAGKGKDSA EGNEEQNDDE DIAREVHVDP NEAAPQQSEK EPLSETDSEW EVERLHHVVL ADFGNSCWTY RQFTDEVQTR QYRSPEVILG YPYSTSIDLW SAACMIFELI TGEFLFDPRK GSDYSRDEDH LALISELLGV LPVSMRLGDG KYRAQYYNSR GELRSIKDLN FWGLEDVLYR KHKFTRKKAK EIAEFLLPML ELEPHNRATA TDMLNNFQHF FEVQEDDYAP LCFVPSSDDH RGGERSSGNM EESEFSDTFE ETDDYDRSRR LDRTYDAMED SLSRQDEETA RYLAGHSLLN EASLAKRGLT ILDIQAVLSG QQLEDEERQA AATEIIRLLS EEADDSSDGH CADREDGDSN SSVDESDSSC TND
NT Sequence
atgccccgac ccgacagaag agcaaaatat gggaggagag cgcaaaataa aataacaacg gggggtggcg gtgcaaagag aaagtcgaaa cccatagcgc cacacgttga aaaagatggt attgacatta atgcactgtt ggaggaaatc gagcgtaacg actcgccggc cgaggagaag gtgcccaaag agaccacgaa aaaatcgcgg aggaggcgca aagccgaaaa agtagaaggt cttgcggtaa aacccggaac cacagtcgcg caagcccgaa tgctagcaag taaaatgcag caaaggcact ccgcttccac gaatgggccc agtcagcctg cccatcagag atcagaagag gacgaagatg aggacgacga tgatgaggaa caggacggta gcggtgagga ctacagcgat atagcaaatg agcgaccgag tgaatatcgt aagggaggct accacccagt cgtggtgggc gaagtttaca atcaacgcta tcgagttgtg cgtaaactcg gatggggtta cttctccact gtgtggctcg tttgggatta tgtggagaaa gtgtttcaag ctatgaaggt gcagaagtcg gccaagcact acacagaagc ggcgtatgac gagatcaaac tactcggaga gatcatgact gcagatccag aaaaagtccg tcggtgtgcg cggttaaatg atcactttga gcagcagggg ccgaacggaa agcacgtttg catggtgttc gacgtctatg gggaggatct actttcattg atcgaacgct ataagtacca tggtgtcccg ctgccgatag ttaagtgtat ttccagacaa atcctcatag ggttggaaca tgtgcattct ttggatatca ttcatacgga tctaaaacca gagaacgtgc tactctcggc accaaaacac gccatcgtat cccaaatgaa acgttttaag cctcctccgc tccacgaccg accgtctttg gtgaaacgcg accccaaaac aatgaccaag tctcaacgcc gtcgctacta caaaaaactt cgcgctgcgg gaaagggtaa ggacagtgcc gaggggaatg aagagcagaa tgatgatgag gacattgcaa gggaggtcca cgtcgaccct aacgaggctg caccgcaaca aagtgagaaa gaaccgttga gcgaaacgga ttctgagtgg gaagttgaac gccttcatca tgtagtgctg gcggattttg gtaatagctg ctggacgtac agacagttta cggatgaagt tcaaactcgt caataccgtt ccccagaagt catattgggt tatccttact ccacctcaat tgatttgtgg tctgccgcct gtatgatctt cgagctgatc acgggtgagt ttctgtttga cccacgaaag ggatctgact attcccgtga tgaagatcat ttagctttga tctctgagtt acttggagta ctaccggtaa gtatgcggct aggtgatggg aagtaccgcg ctcagtacta caactcacgg ggtgaacttc gtagcataaa ggatcttaat ttctggggct tggaggatgt tctctaccga aaacacaagt tcacacgcaa aaaagcgaaa gaaatagcgg agttcctttt accaatgctc gaattggaac ctcataatcg agcaacagcc accgatatgc ttaataattt tcagcacttt tttgaggtac aagaggatga ttacgcacca ctttgcttcg taccttcttc ggatgatcac cgtggtgggg agcggagctc aggaaatatg gaggagtcag agttcagcga tacctttgaa gaaaccgatg actacgatcg ctcccgaaga ctcgacagaa cgtatgatgc aatggaagat tctctgagca ggcaggacga ggagactgca cggtatttgg ctggtcattc cctgctcaac gaggcatcct tggcaaaacg tgggttaaca attttagata ttcaagccgt tctatccggg caacaactgg aggatgagga aaggcaagct gctgccactg aaattattcg tcttctctca gaggaagcgg atgacagcag cgatggacac tgcgcagaca gggaagacgg ggattcaaac tcatctgtag atgaatctga cagcagctgc actaatgact aa
Details for TrbrA.20382.a.HZ2.GE43759
HARVESTED ON: data unavailable
SEQUENCED ON: 6/23/2020
EXPECTED MW: 66kDa
OBSERVED MW: 75kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSEQ DGSGEDYSDI ANERPSEYRK GGYHPVVVGE VYNQRYRVVR KLGWGYFSTV WLVWDYVEKV FQAMKVQKSA KHYTEAAYDE IKLLGEIMTA DPEKVRRCAR LNDHFEQQGP NGKHVCMVFD VYGEDLLSLI ERYKYHGVPL PIVKCISRQI LIGLEHVHSL DIIHTDLKPE NVLLSAPKHA IVSQMKRFKP PPLHDRPSLV KRDPKTMTKS QRRRYYKKLR AAGKGKDSAE GNEEQNDDED IAREVHVDPN EAAPQQSEKE PLSETDSEWE VERLHHVVLA DFGNSCWTYR QFTDEVQTRQ YRSPEVILGY PYSTSIDLWS AACMIFELIT GEFLFDPRKG SDYSRDEDHL ALISELLGVL PVSMRLGDGK YRAQYYNSRG ELRSIKDLNF WGLEDVLYRK HKFTRKKAKE IAEFLLPMLE LEPHNRATAT DMLNNFQHFF EVQEDDYAPL CFV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcgg aacaggacgg tagcggtgag gactacagcg atatagcaaa tgagcgaccg agtgaatatc gtaagggagg ctaccaccca gtcgtggtgg gcgaagttta caatcaacgc tatcgagttg tgcgtaaact cggatggggt tacttctcca ctgtgtggct cgtttgggat tatgtggaga aagtgtttca agctatgaag gtgcagaagt cggccaagca ctacacagaa gcggcgtatg acgagatcaa actactcgga gagatcatga ctgcagatcc agaaaaagtc cgtcggtgtg cgcggttaaa tgatcacttt gagcagcagg ggccgaacgg aaagcacgtt tgcatggtgt tcgacgtcta tggggaggat ctactttcat tgatcgaacg ctataagtac catggtgtcc cgctgccgat agttaagtgt atttccagac aaatcctcat agggttggaa catgtgcatt ctttggatat cattcatacg gatctaaaac cagagaacgt gctactctcg gcaccaaaac acgccatcgt atcccaaatg aaacgtttta agcctcctcc gctccacgac cgaccgtctt tggtgaaacg cgaccccaaa acaatgacca agtctcaacg ccgtcgctac tacaaaaaac ttcgcgctgc gggaaagggt aaggacagtg ccgaggggaa tgaagagcag aatgatgatg aggacattgc aagggaggtc cacgtcgacc ctaacgaggc tgcaccgcaa caaagtgaga aagaaccgtt gagcgaaacg gattctgagt gggaagttga acgccttcat catgtagtgc tggcggattt tggtaatagc tgctggacgt acagacagtt tacggatgaa gttcaaactc gtcaataccg ttccccagaa gtcatattgg gttatcctta ctccacctca attgatttgt ggtctgccgc ctgtatgatc ttcgagctga tcacgggtga gtttctgttt gacccacgaa agggatctga ctattcccgt gatgaagatc atttagcttt gatctctgag ttacttggag tactaccggt aagtatgcgg ctaggtgatg ggaagtaccg cgctcagtac tacaactcac ggggtgaact tcgtagcata aaggatctta atttctgggg cttggaggat gttctctacc gaaaacacaa gttcacacgc aaaaaagcga aagaaatagc ggagttcctt ttaccaatgc tcgaattgga acctcataat cgagcaacag ccaccgatat gcttaataat tttcagcact tttttgaggt acaagaggat gattacgcac cactttgctt cgtataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa