SSGCID: Target Page

TrbrA.20202.a

tRNA synthetases class II (A)/Threonyl and Alanyl tRNA synthetase second additional domain containing protein, putative

CENTER ID:	TrbrA.20202.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.20202.a.B1.GE42778	full length		1	468

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.20202.a.B1.PW38593	full length		1	468

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:Tb927.9.6650
UniProt:	Q38ES2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKTVISTAFA RVGELACQQD SFLREITARV VSCEAAAVNR NNSGASGKKS KSSGTQVELP QSYDVILTDS VLFPDGGGQP CDHGVLIRRE TNEELPVRVV QRRGDSCVMN LPCMLKVGED VQLRVDWDRR LDHMQHHSAQ HLLTAVVEDP SFCGLPTVSW ALTQPYCHIV LPTGKRIEPE MLQRIEEHCN RVIARAVPVR CRVYSSKEEY ENDLQREPEK NDDGGRLRGS RPIPPDVAGP IRIIDMEGID CCTCCGTHVT NLAQLQVLKL LHQETKGDTL KLFFIAGERV RRFFGDMYGR ERELMKEMGG IRPEDFVSAA IRKGKDFVEL EKRLKHMTQE LMKLRSEKLI AEAKQLSENG KGDSASHRPP VVVYRRDDVD ADFFSGLRDE LRQACPNCVG VFAWAMAPLT AAKTGQFMIV GPTESVEVLA PVVCAALEGK GGMSKFGYRG KGSLCAWEDL VKELTSKG

NT Sequence

atgaagactg taatttccac tgcttttgca cgcgttggtg agcttgcttg tcagcaggat tcattcctgc gtgagataac cgctcgtgtc gtatcctgcg aggctgccgc tgtgaacaga aacaatagtg gtgcctccgg taaaaagagc aaaagtagcg gtacacaagt ggagctgccg caaagctacg acgtgatact caccgactct gttctgtttc ccgacggtgg tgggcaaccg tgcgaccatg gtgttcttat ccggcgagaa acaaacgagg aactgccggt ccgtgtggtt cagcggcgag gtgattcgtg tgtgatgaat ttgccgtgca tgctcaaagt cggtgaggac gtccaactcc gggttgactg ggaccgccgt ctcgaccaca tgcagcacca ctccgcgcag catttgttga ctgctgtcgt tgaagacccc agtttctgtg gtttgccaac agtgtcgtgg gcgctaacgc agccatactg tcatatcgtt cttccaacag gaaaaaggat tgagcctgag atgttgcagc gaatcgaaga gcactgcaat cgtgtcattg cgcgggcggt tcctgtacgg tgcagagtat acagcagcaa agaggagtat gaaaacgact tgcagaggga gccggagaaa aatgacgacg gaggaaggct gcgcggctcg cgacccattc ctccagatgt tgcggggccc attcgtataa ttgatatgga gggtattgac tgttgcactt gttgtggcac tcacgtcacc aacttggcgc agttgcaagt attgaagctc ctacaccaag aaaccaaagg agatacactg aaactgttct tcattgcagg tgaacgcgtg aggcgtttct tcggcgacat gtatggtcgc gagagggagc taatgaaaga aatgggtggc atacggcctg aagatttcgt ttcggcagcc attcgaaagg gaaaagactt cgtggagctg gagaaacgat tgaagcacat gactcaagag cttatgaaac tgcggagcga gaaacttatc gcagaggcaa aacagttgtc agaaaacggg aagggtgaca gtgccagtca caggcctcct gttgtcgttt accgtcgcga tgatgttgac gcagatttct tcagcggact tcgggatgaa ctgcgacaag cgtgtcccaa ctgtgtcggt gtctttgctt gggcaatggc tcctctaacc gcagccaaaa cggggcaatt tatgattgtt ggcccaactg aaagtgtaga ggttttggcg ccggttgtgt gtgcagctct cgagggtaag ggaggcatgt cgaagtttgg ctaccgtgga aagggaagcc tttgcgcttg ggaagatctc gtgaaggagt tgacttccaa ggggtaa

Details for TrbrA.20202.a.B1.GE42778

HARVESTED ON:	11/5/2018
SEQUENCED ON:	11/7/2018
EXPECTED MW:	53kDa
OBSERVED MW:	53kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	96
PERCENT COVERAGE:	48

Validated AA Sequence

MAHHHHHHMK TVISTAFARV GELACQQDSF LREITARVVS CEAAAVNRNN SGASGKKSKS SGTQVELPQS YDVILTDSVL FPDGGGQPCD HGVLIRRETN EELPVRVVQR RGDLCVMNLP CMLKVGEDVQ LRVDWDRRLD HMQHHSAQHL LTAVVEDPGF CGLPTVSWAL TQPYCHIVLP TGKRIEPEMV QRIEEHCNRV IAGAVPVRCR VYXSKEEYEN XXAEGAGEK

Validated NT Sequence

cgagatcttc ccaagcgcaa aggcttccct ttccacggta gccaaacttc gacatgcctc ccttaccctc gagagctgca cacacaaccg gcgccaaaac ctctacactt tcagttgggc caacaatcat aaattgcccc gttttggctg cggttagagg agccattgcc caagcaaaga caccgacaca gttgggacac gcttgtcgca gttcatcccg aagtccgctg aagaaatctg cgtcaacatc atcgcgacgg taaacgacaa caggaggcct gtgactggca ctgccaccct tcccgttttc tgacaactgt tttgcctctg cgataagttt ctcgctccgc agtttcataa gctcttgagt catgtgcttc aatcgtttct ccagctccac gaagtctttt ccctttcgaa tggctgccga aacgaaatct tcaggccgta tgccacccat ttctttcatt agctccctct cgcgaccata catgtcgccg aagaaacgcc tcacgcgttc acctgcaatg aagaacagtt tcagtgtatc tcctttggtt tcttggtgta ggagcttcaa tacttgcaac nncgccaagt tggtgacgtg agtgccacaa caagtgcaac agtcaatacc ctccatatca attatacgaa tgggccccgc aacatctgga ggaatgggtc gcgagccgcg cannntnnnt ccgtcgtcat ttttctccgg ctccctctgc aannncgttt tcatactcct ctttgctnnt gtatactctg caccgtacag gaaccgcccc cgcaatgaca cgattgcagt gctcttcgat tcgctgcacc atctcaggct caatcctttt tcctgttgga agaacgatat gacagtatgg ctgcgttagc gcccacgaca ctgttggcaa accacagaaa ccggggtctt caacgacagc agtcaacaaa tgctgcgcgg agtggtgctg catgtggtcg agacggcggt cccagtcaac ccggagttgg acgtcctcac cgactttgag catgcacggc aaattcatca cacacaaatc acctcgccgc tgaaccacac ggaccggcag ttcctcgttt gtttctcgcc ggataagaac accatggtcg cacggttgcc caccaccgtc gggaaacaga acagagtcgg tgagtatcac gtcgtagctt tgcggcagct ccacttgtgt accgctactt ttgctctttt taccagaggc accactattg tttctgttca cagcggcagc ctcgcaggat acgacacgag cggttatctc acgcaggaat gaatcctgct gacaagcaag ttcaccaacg cgtgcaaaag cagtggaaat tacagtcttc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

MAHHHHHHMK TVISTAFARV GELACQQDSF LREITARVVS CEAAAVNRNN SGASGKKSKS SGTQVELPQS YDVILTDSVL FPDGGGQPCD HGVLIRRETN EELPVRVVQR RGDSCVMNLP CMLKVGEDVQ LRVDWDRRLD HMQHHSAQHL LTAVVEDPSF CGLPTVSWAL TQPYCHIVLP TGKRIEPEML QRIEEHCNRV IARAVPVRCR VYSSKEEYEN DLQREPEKND DGGRLRGSRP IPPDVAGPIR IIDMEGIDCC TCCGTHVTNL AQLQVLKLLH QETKGDTLKL FFIAGERVRR FFGDMYGRER ELMKEMGGIR PEDFVSAAIR KGKDFVELEK RLKHMTQELM KLRSEKLIAE AKQLSENGKG DSASHRPPVV VYRRDDVDAD FFSGLRDELR QACPNCVGVF AWAMAPLTAA KTGQFMIVGP TESVEVLAPV VCAALEGKGG MSKFGYRGKG SLCAWEDLVK ELTSKG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaga ctgtaatttc cactgctttt gcacgcgttg gtgagcttgc ttgtcagcag gattcattcc tgcgtgagat aaccgctcgt gtcgtatcct gcgaggctgc cgctgtgaac agaaacaata gtggtgcctc cggtaaaaag agcaaaagta gcggtacaca agtggagctg ccgcaaagct acgacgtgat actcaccgac tctgttctgt ttcccgacgg tggtgggcaa ccgtgcgacc atggtgttct tatccggcga gaaacaaacg aggaactgcc ggtccgtgtg gttcagcggc gaggtgattc gtgtgtgatg aatttgccgt gcatgctcaa agtcggtgag gacgtccaac tccgggttga ctgggaccgc cgtctcgacc acatgcagca ccactccgcg cagcatttgt tgactgctgt cgttgaagac cccagtttct gtggtttgcc aacagtgtcg tgggcgctaa cgcagccata ctgtcatatc gttcttccaa caggaaaaag gattgagcct gagatgttgc agcgaatcga agagcactgc aatcgtgtca ttgcgcgggc ggttcctgta cggtgcagag tatacagcag caaagaggag tatgaaaacg acttgcagag ggagccggag aaaaatgacg acggaggaag gctgcgcggc tcgcgaccca ttcctccaga tgttgcgggg cccattcgta taattgatat ggagggtatt gactgttgca cttgttgtgg cactcacgtc accaacttgg cgcagttgca agtattgaag ctcctacacc aagaaaccaa aggagataca ctgaaactgt tcttcattgc aggtgaacgc gtgaggcgtt tcttcggcga catgtatggt cgcgagaggg agctaatgaa agaaatgggt ggcatacggc ctgaagattt cgtttcggca gccattcgaa agggaaaaga cttcgtggag ctggagaaac gattgaagca catgactcaa gagcttatga aactgcggag cgagaaactt atcgcagagg caaaacagtt gtcagaaaac gggaagggtg acagtgccag tcacaggcct cctgttgtcg tttaccgtcg cgatgatgtt gacgcagatt tcttcagcgg acttcgggat gaactgcgac aagcgtgtcc caactgtgtc ggtgtctttg cttgggcaat ggctcctcta accgcagcca aaacggggca atttatgatt gttggcccaa ctgaaagtgt agaggttttg gcgccggttg tgtgtgcagc tctcgagggt aagggaggca tgtcgaagtt tggctaccgt ggaaagggaa gcctttgcgc ttgggaagat ctcgtgaagg agttgacttc caaggggtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for TrbrA.20202.a.B1.PW38593

PURIFICATION DATe:	1/22/2019
CONCENTRATION:	23.29mg/ml
OBSERVED MW:	52kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 8, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	15
VIAL VOLUME:	200µl
PERCENT IDENTITY:	96
PERCENT COVERAGE:	48

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAGA CTGTAATTTC CACTGCTTTT GCACGCGTTG GTGAGCTTGC TTGTCAGCAG GATTCATTCC TGCGTGAGAT AACCGCTCGT GTCGTATCCT GCGAGGCTGC CGCTGTGAAC AGAAACAATA GTGGTGCCTC CGGTAAAAAG AGCAAAAGTA GCGGTACACA AGTGGAGCTG CCGCAAAGCT ACGACGTGAT ACTCACCGAC TCTGTTCTGT TTCCCGACGG TGGTGGGCAA CCGTGCGACC ATGGTGTTCT TATCCGGCGA GAAACAAACG AGGAACTGCC GGTCCGTGTG GTTCAGCGGC GAGGTGATTC GTGTGTGATG AATTTGCCGT GCATGCTCAA AGTCGGTGAG GACGTCCAAC TCCGGGTTGA CTGGGACCGC CGTCTCGACC ACATGCAGCA CCACTCCGCG CAGCATTTGT TGACTGCTGT CGTTGAAGAC CCCAGTTTCT GTGGTTTGCC AACAGTGTCG TGGGCGCTAA CGCAGCCATA CTGTCATATC GTTCTTCCAA CAGGAAAAAG GATTGAGCCT GAGATGTTGC AGCGAATCGA AGAGCACTGC AATCGTGTCA TTGCGCGGGC GGTTCCTGTA CGGTGCAGAG TATACAGCAG CAAAGAGGAG TATGAAAACG ACTTGCAGAG GGAGCCGGAG AAAAATGACG ACGGAGGAAG GCTGCGCGGC TCGCGACCCA TTCCTCCAGA TGTTGCGGGG CCCATTCGTA TAATTGATAT GGAGGGTATT GACTGTTGCA CTTGTTGTGG CACTCACGTC ACCAACTTGG CGCAGTTGCA AGTATTGAAG CTCCTACACC AAGAAACCAA AGGAGATACA CTGAAACTGT TCTTCATTGC AGGTGAACGC GTGAGGCGTT TCTTCGGCGA CATGTATGGT CGCGAGAGGG AGCTAATGAA AGAAATGGGT GGCATACGGC CTGAAGATTT CGTTTCGGCA GCCATTCGAA AGGGAAAAGA CTTCGTGGAG CTGGAGAAAC GATTGAAGCA CATGACTCAA GAGCTTATGA AACTGCGGAG CGAGAAACTT ATCGCAGAGG CAAAACAGTT GTCAGAAAAC GGGAAGGGTG ACAGTGCCAG TCACAGGCCT CCTGTTGTCG TTTACCGTCG CGATGATGTT GACGCAGATT TCTTCAGCGG ACTTCGGGAT GAACTGCGAC AAGCGTGTCC CAACTGTGTC GGTGTCTTTG CTTGGGCAAT GGCTCCTCTA ACCGCAGCCA AAACGGGGCA ATTTATGATT GTTGGCCCAA CTGAAAGTGT AGAGGTTTTG GCGCCGGTTG TGTGTGCAGC TCTCGAGGGT AAGGGAGGCA TGTCGAAGTT TGGCTACCGT GGAAAGGGAA GCCTTTGCGC TTGGGAAGAT CTCGTGAAGG AGTTGACTTC CAAGGGGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT