SSGCID: Target Page

TrbrA.20196.a

PNUTS

CENTER ID:	TrbrA.20196.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.20196.a.B1.GE42368	full length		1	325

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:Tb927.10.11960
UniProt:	Q389E6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKYLENTTTI VKPNGKKNGL TKSSSECINY SLCEGADCNA RADLQEGGAS CPCSPPLAAE RMGCEQQKYA ASSLGDGQHR GKRGRGGEVS NGNCENVLPE ASPCVSAEVS ASSIVHSAVA VGASPVTLES RKETERRGKR ISWHDDENGG VESDSLSSSS LRSGAKRRID NMFIYPREQL SLPKVITENT PYCERQNDDG TSYISYKLQG PSRRFTMWRQ RPVSGDCEGR KESKFEDQPC GNRPHKRINS LFSSIYESYD PKSDEQTVQL RIATFDEESE VNGALCAGNL QMPFACVSRI TNDPVPIPLA PSTEPKNAQV NGQHS

NT Sequence

atgaagtacc tggagaatac cactactatt gtaaaaccaa atgggaagaa aaatggcctt acgaaatcgt ctagtgaatg tatcaattat tccttgtgtg agggcgctga ctgcaacgca agggcagatt tgcaggaggg aggcgctagc tgcccttgtt caccaccgct agctgcggaa agaatgggtt gtgaacagca aaaatacgcc gcatcatctc ttggagatgg acaacataga ggaaaacgcg ggcgcggtgg cgaagttagt aacgggaact gcgagaatgt tttacctgaa gcatctccgt gtgtgagcgc ggaggtatca gcttcttcaa ttgttcacag tgctgtcgcc gtgggagcat cccccgtcac acttgagtcg aggaaagaga cggaacggag gggtaaacgc ataagttggc acgacgacga aaacggtggc gtggagtctg actctttgag ctcatcgtcc ctccgctcgg gtgctaagag gagaattgac aacatgttca tttacccgag ggaacaactt agtttaccca aggtaattac agaaaataca ccctactgcg aaaggcaaaa cgacgatgga acatcttaca tctcgtacaa gcttcagggc ccttcccgtc gtttcacaat gtggagacaa cgaccggtat caggagattg tgaaggtaga aaggaatcga aatttgagga tcaaccatgt ggtaatcgac cgcacaaaag gattaactcg ctattttcat ccatatatga atcgtacgat cccaaatctg atgagcaaac tgtgcagctg cgtatagcaa cgtttgatga ggaatctgag gttaatggcg ctttgtgcgc tggtaatttg cagatgccgt ttgcatgcgt gtcgcgtatc actaacgacc cagtgccaat tccgcttgcg ccttcaactg aaccaaagaa cgctcaagta aatgggcaac acagttaa

Details for TrbrA.20196.a.B1.GE42368

HARVESTED ON:	4/13/2018
SEQUENCED ON:	4/18/2018
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMK YLENTTTIVK PNGKKNGLTK SSSECINYSV CEGTDCNARA DLQGGGASCP CSPRLAAGRM GCEQQKYAAS SLGDGQHRGK RGRGGEVSNG NCENVLPEAS PCVSAEVSAS SIVHSAVTVG ASPVTLESRK ETERRGKRIS WHDDENGGVE SDSLSSSSLR SGAKRRIDNM FIYPREQLSL PKVITENIPY CERQNDDGTS YISYKLQGPS RRFTMWRQRP VSGDCEGRKE SKFEAQPCGN RPHKRINSLF SSIYESYDPK SDEQTVQLRI ATFDEESEVN GALCAGNLQM PFACVSRITN DPVPIPLAPS TEPK

Validated NT Sequence

ttctttggtt cagttgaagg cgcaagcgga attggcactg ggtcgttagt gatacgcgac acgcatgcaa acggcatctg caaattacca gcgcacaaag cgccattaac ctcagattcc tcatcaaacg ttgctatacg cagctgcaca gtttgctcat cagatttggg atcgtacgat tcatatatgg atgaaaatag cgagttaatc cttttgtgcg gtcgattacc acatggttga gcctcaaatt tcgattcctt tctaccttca caatctcccg ataccggtcg ttgtctccac attgtgaaac gacgggaagg gccctgaagc ttgtacgaga tgtaagatgt tccatcgtcg ttttgccttt cgcagtaggg tatattttct gtaattacct tgggtaaact aagttgttcc ctcgggtaaa tgaacatgtt gtcaattctc ctcttagcac ccgagcggag ggacgatgag ctcaaagagt cagactccac gccaccgttt tcgtcgtcgt gccaacttat gcgtttaccc ctccgttccg tctctttcct cgactcaagt gtgacggggg atgctcccac ggtgacagca ctgtgaacaa ttgaagaagc tgatacctcc gcgctcacac acggagatgc ttcaggtaaa acgttctcgc agttcccgtt actaacttcg ccaccgcgcc cgcgttttcc tctatgttgt ccatctccaa gagatgatgc ggcgtatttt tgctgttcac aacccattct tcccgcagct agccgcggtg aacaagggca gctagcgcct cccccctgca aatctgccct tgcgttgcag tcagtgccct cacacacgga ataattgata cattcactag acgatttcgt aaggccattt ttcttcccat ttggttttac aatagtagtg gtattctcca ggtacttcat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

MAHHHHHHMK YLENTTTIVK PNGKKNGLTK SSSECINYSL CEGADCNARA DLQEGGASCP CSPPLAAERM GCEQQKYAAS SLGDGQHRGK RGRGGEVSNG NCENVLPEAS PCVSAEVSAS SIVHSAVAVG ASPVTLESRK ETERRGKRIS WHDDENGGVE SDSLSSSSLR SGAKRRIDNM FIYPREQLSL PKVITENTPY CERQNDDGTS YISYKLQGPS RRFTMWRQRP VSGDCEGRKE SKFEDQPCGN RPHKRINSLF SSIYESYDPK SDEQTVQLRI ATFDEESEVN GALCAGNLQM PFACVSRITN DPVPIPLAPS TEPKNAQVNG QHS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagt acctggagaa taccactact attgtaaaac caaatgggaa gaaaaatggc cttacgaaat cgtctagtga atgtatcaat tattccttgt gtgagggcgc tgactgcaac gcaagggcag atttgcagga gggaggcgct agctgccctt gttcaccacc gctagctgcg gaaagaatgg gttgtgaaca gcaaaaatac gccgcatcat ctcttggaga tggacaacat agaggaaaac gcgggcgcgg tggcgaagtt agtaacggga actgcgagaa tgttttacct gaagcatctc cgtgtgtgag cgcggaggta tcagcttctt caattgttca cagtgctgtc gccgtgggag catcccccgt cacacttgag tcgaggaaag agacggaacg gaggggtaaa cgcataagtt ggcacgacga cgaaaacggt ggcgtggagt ctgactcttt gagctcatcg tccctccgct cgggtgctaa gaggagaatt gacaacatgt tcatttaccc gagggaacaa cttagtttac ccaaggtaat tacagaaaat acaccctact gcgaaaggca aaacgacgat ggaacatctt acatctcgta caagcttcag ggcccttccc gtcgtttcac aatgtggaga caacgaccgg tatcaggaga ttgtgaaggt agaaaggaat cgaaatttga ggatcaacca tgtggtaatc gaccgcacaa aaggattaac tcgctatttt catccatata tgaatcgtac gatcccaaat ctgatgagca aactgtgcag ctgcgtatag caacgtttga tgaggaatct gaggttaatg gcgctttgtg cgctggtaat ttgcagatgc cgtttgcatg cgtgtcgcgt atcactaacg acccagtgcc aattccgctt gcgccttcaa ctgaaccaaa gaacgctcaa gtaaatgggc aacacagttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat