SSGCID: Target Page

TrbrA.19955.a

DNA topoisomerase II

CENTER ID:	TrbrA.19955.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.19955.a.B4.GE43847	ordered domain		1	1123

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:Tb927.9.5590
UniProt:	Q38EZ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAEAHKYKKL TPIEHVLTRP EMYIGSLDTT ATPMFIYDEQ KGHMVWETVK LNHGLLKIVD EILLNASDNI SNRSARMTYI RVTITDTGEI TIENDGAGIP IVRSREHKLY IPEMVFGHLL TSSNYDDDNQ NAVAGRHGYG AKLTNILSLS FSVCCRTNGR EFHMSWQDHM RKATAPRVSN VGTKEKNVTR VKFLPDYERF GMKEKKISND MKRVLYKRIM DLSAMFPNIQ ITLNGSSFGF KSFKDYATLY SAMTPKGEKP PPPYVYESKS GCVAFIPSVV PGVRRMFGVV NGVVTYNGGT HCNAAQDILT GCLDGVEREL KKENKVMDTN RVLRHFTILV FLVQVQPKFD SQNKARLVST PTMPRVPRQD VMKYLLRMPF LEAHVSTITG QLAQELNKEI GTGRRMSSKT LLTSITKLVD ATSTRRDPKH TRTLIVTEGD SAKALAQNSL SSDQKRYTGV FPLRGKLLNV RNKNLKRLRN CKELQELFCA LGLELDKDYT DADELRYQRI LIMTDQDADG SHIKGLVINA FESLWPSLLV RNPGFISIFS TPIVKARLRD KSVVSFFSMK EFHKWQRSNA NTPYTCKYYK GLGTSTTAEG KEYFKDMEKH TMRLLVDRSD HKLLDNVFDS QEVEWRKDWM TKANAFTGEV DIDRSKKMLT VTDFVHKEMV HFALVGNARA LAHSVDGLKP SQRKIIWALM RRSGNEAAKV AQLSGYISEA SAFHHGETSL QETMIKMAQS FTGGNNVNLL VPEGQFGSRQ QLGNDHAAPR YIFTKLSKVA RLLFPSEDDP LLDYIVEEGQ QVEPNHYVPI LPLLLCNGSV GIGFGFASNI PPFHPLDVSA AVRAMISGEA AKSVVRRLVP WAVGFQGEIR RGPEGEFIAV GTYTYCKGGR VHVTELPWTC SVEAFREHIS YLATKDIVNR IADYSGANHV DIDVEVAQGA VNTYAECESE LGLTQRIHIN GTVFSPNGTL SPLESDLTPV LQWHYDRRLD LYKKRRQRNL MLLEQELARE KSTLKFVQHF RAGHIDFANA TEATLEKVCS KLGLVRVDDS FDYILRKPIT FYTKTSFENL LKKIAETERR IEALKKTTPV QLWLGELDQF DRFFQDHEKK MVEAILKERR QRSPPSDLLP GLQQPRLEVE EAKGGKKFEM RVQVRKYVPP PTKRGAGGRS DGDGGATAAG AAAAVGGRGE KKGPGRAGGV RRMVLDALAK RVTRLLPRLL F

NT Sequence

atggcggagg cacacaagta taagaagctc acacctattg agcatgtact cacacgacca gagatgtaca ttggtagtct cgacacaacg gcaaccccca tgtttatata cgatgaacag aagggtcaca tggtgtggga gacggtgaaa ctgaatcacg gtttgctgaa aatcgtggat gaaattctgc taaatgcatc tgataacatc tccaacagaa gtgcgcgcat gacgtatatc cgcgtgacca tcacggacac gggtgagatt actatagaga acgacggcgc tgggatcccc atcgtacgca gtcgagagca taaattatat ataccagaga tggtattcgg tcacctactt accagctcta attatgatga cgataaccaa aatgcagttg ctggtcgcca cggttacggt gcaaagctaa ccaacattct ttccctgagc ttttccgtct gctgccgcac aaatgggagg gagtttcaca tgagttggca ggatcacatg aggaaggcaa cggctccacg cgtttcaaac gtcggcacaa aagagaaaaa tgtcacgcgt gtgaagtttc tccccgacta cgagcgattt ggcatgaagg agaagaaaat ttcaaacgac atgaagcgtg tgctctacaa gcgcattatg gacctttccg caatgtttcc gaatattcaa ataaccctga acggctcatc ctttggcttc aagtccttta aggactatgc gactctgtac agtgccatga ccccaaaggg agagaaacca ccgccaccat acgtatacga gagtaaaagc ggttgcgttg ccttcattcc ttcagtagtc cccggggtgc ggcggatgtt tggtgtggtc aacggtgtgg taacgtataa tggcggtacg cattgcaatg ctgcgcagga tatattgacc ggctgcctcg atggcgtgga acgggaatta aagaaggaga acaaagtgat ggacactaat cgagtgcttc gtcacttcac tattctagtt ttcctcgtgc aggtgcagcc aaagtttgat tctcagaaca aagctcgact tgtttctacc cccacgatgc cccgtgttcc tcggcaagat gtgatgaaat atcttctgcg catgcctttt ctcgaggctc atgtgagtac tattacggga cagttagcgc aggaactaaa taaggagatc ggcaccggac gccgtatgag tagcaaaacc ctcctgacct ccataacgaa actggtagat gcaacttcta cacgccgtga cccaaaacat acccgcacgt taattgttac tgaaggtgac tccgcaaagg ctctcgcgca gaactcttta tcgagtgacc aaaagcgata tacaggcgta tttccgcttc ggggtaagct gctaaacgtg cgtaacaaga atcttaagcg actgaggaac tgcaaggagt tgcaggaact gttttgtgcc ctgggacttg agctagataa agattacacc gacgccgatg aactacgata ccaacgcata cttatcatga cagatcagga cgcagatggc tcacacatta agggtttggt tatcaacgcg ttcgagtctt tgtggccctc gttgctggta cgaaatcctg ggttcatctc tatattctcc acacccatcg taaaggcacg actgcgcgac aagtcggtgg tatccttctt cagcatgaag gagtttcaca agtggcagcg ctcaaatgca aatacaccat acacatgtaa gtactataag ggtctcggta cttctaccac tgctgaggga aaagagtact tcaaggatat ggagaaacac acaatgcgct tactcgtgga ccgctccgat cataagcttc ttgacaatgt tttcgactca caggaggtag aatggcgaaa ggactggatg accaaggcga atgcttttac cggcgaggtg gatattgatc gtagcaagaa aatgctaacg gtcacagatt ttgtgcataa ggagatggtt catttcgccc ttgttggtaa tgcccgtgcg cttgcgcact ctgtagacgg gcttaagcct tctcagcgaa agattatttg ggctcttatg cggcggtccg gtaatgaggc ggcgaaggtg gcacaactat caggttacat atcagaagct tccgcttttc atcatggtga gacttcattg caggagacga tgattaagat ggcgcagagc ttcactggtg gtaacaacgt caaccttctc gtccctgagg gtcagttcgg ttctcgtcag caactcggta atgatcatgc ggcaccccgt tacattttca caaagctttc aaaagtagcc cgcttgcttt tccctagtga ggatgaccca ttgctagact acattgtgga agagggtcag caggtggagc cgaaccatta cgttccaatc ctaccgctgc tcctctgcaa cggaagtgtg ggcatcggtt tcggttttgc gtcgaatatt ccaccattcc acccgttgga cgtatctgca gcggtacgag cgatgattag cggcgaagct gccaagtcgg ttgtccgtcg acttgtgccg tgggctgtag gctttcaggg tgagatacgt cgtggccccg aaggagagtt tattgctgtg ggaacgtata cttactgtaa gggtggtcgt gtgcatgtta cggagcttcc ttggacgtgt agcgttgaag cattccgtga gcacatttct tacctcgcca caaaggatat tgttaaccgc attgccgact attccggcgc caatcacgtt gacattgatg tggaagttgc tcagggtgcg gtgaacacat atgctgagtg cgagtcggaa cttggcctca cgcaacgtat tcacatcaac ggtacagtct tttcaccgaa tggaactctt tcacctctgg aaagtgacct cacacccgtc ctccagtggc actacgaccg cagacttgat ttatataaaa agaggcgaca acgtaatttg atgctgttgg agcaggaatt ggccagagag aagtcgacac tcaaatttgt gcaacacttc cgtgccggcc acattgactt tgcgaatgct acggaggcaa cacttgaaaa ggtgtgttca aagttagggt tagtacgtgt agatgactcg ttcgactaca ttttgcgtaa acccatcacg ttctatacca aaacaagttt tgaaaatctt ctcaagaaga tcgcggagac ggagcggcgc attgaagctc tcaagaagac aacccctgtg cagttgtggt tgggcgaact tgaccaattt gatcgcttct ttcaggacca tgagaaaaag atggtggagg ctattttgaa ggaaagaagg cagcgatcac ccccgagtga ccttctccct ggtcttcaac agccgcgtct ggaggtggag gaggcaaagg gtggtaaaaa atttgagatg cgggttcagg tgcgaaagta cgtccctcca ccaaccaagc ggggagcggg gggccgtagt gatggtgacg gaggcgcaac tgcggccggt gctgctgcag ccgtgggggg aagaggtgaa aagaagggcc cgggacgagc cggtggggtt cgacgtatgg tactcgacgc ccttgcgaag cgtgtgacac gtttgctgcc gcgtctgctg ttctag

Details for TrbrA.19955.a.B4.GE43847

HARVESTED ON:	8/25/2020
SEQUENCED ON:	8/28/2020
EXPECTED MW:	128kDa
OBSERVED MW:	128kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMA EAHKYKKLTP IEHVLTRPEM YIGSLDTTAT PMFIYDEQKG HMVWETVKLN HGLLKIVDEI LLNASDNISN RSARMTYIRV TITDTGEITI ENDGAGIPIV RSREHKLYIP EMVFGHLLTS SNYDDDNQNA VAGRHGYGAK LTNILSLSFS VCCRTNGREF HMSWQDHMRK ATAPRVSNVG TKEKNVTRVK FLPDYERFGM KEKKISNDMK RVLYKRIMDL SAMFPNIQIT LNGSSFGFKS FKDYATLYSA MTPKGEKPPP PYVYESKSGC VAFIPSVVPG VRRMFGVVNG VVTYNGGTHC NAAQDILTGC LDGVERELKK ENKVMDTNRV LRHFTILVFL VQVQPKFDSQ NKARLVSTPT MPRVPRQDVM KYLLRMPFLE AHVSTITGQL AQELNKEIGT GRRMSSKTLL TSITKLVDAT STRRDPKHTR TLIVTEGDSA KALAQNSLSS DQKRYTGVFP LRGKLLNVRN KNLKRLRNCK ELQELFCALG LELDKDYTDA DELRYQRILI MTDQDADGSH IKGLVINAFE SLWPSLLVRN PGFISIFSTP IVKARLRDKS VVSFFSMKEF HKWQRSNANT PYTCKYYKGL GTSTTAEGKE YFKDMEKHTM RLLVDRSDHK LLDNVFDSQE VEWRKDWMTK ANAFTGEVDI DRSKKMLTVT DFVHKEMVHF ALVGNARALA HSVDGLKPSQ RKIIWALMRR SGNEAAKVAQ LSGYISEASA FHHGETSLQE TMIKMAQSFT GGNNVNLLVP EGQFGSRQQL GNDHAAPRYI FTKLSKVARL LFPSEDDPLL DYIVEEGQQV EPNHYVPILP LLLCNGSVGI GFGFASNIPP FHPLDVSAAV RAMISGEAAK SVVRRLVPWA VGFQGEIRRG PEGEFIAVGT YTYCKGGRVH VTELPWTCSV EAFREHISYL ATKDIVNRIA DYSGANHVDI DVEVAQGAVN TYAECESELG LTQRIHINGT VFSPNGTLSP LESDLTPVLQ WHYDRRLDLY KKRRQRNLML LEQELAREKS TLKFVQHFRA GHIDFANATE ATLEKVCSKL GLVRVDDSFD YILRKPITFY TKTSFENLLK KIAETERRIE ALKKTTPVQL WLGELDQFDR FFQDHEKKMV EAILKERRQR S

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgg aggcacacaa gtataagaag ctcacaccta ttgagcatgt actcacacga ccagagatgt acattggtag tctcgacaca acggcaaccc ccatgtttat atacgatgaa cagaagggtc acatggtgtg ggagacggtg aaactgaatc acggtttgct gaaaatcgtg gatgaaattc tgctaaatgc atctgataac atctccaaca gaagtgcgcg catgacgtat atccgcgtga ccatcacgga cacgggtgag attactatag agaacgacgg cgctgggatc cccatcgtac gcagtcgaga gcataaatta tatataccag agatggtatt cggtcaccta cttaccagct ctaattatga tgacgataac caaaatgcag ttgctggtcg ccacggttac ggtgcaaagc taaccaacat tctttccctg agcttttccg tctgctgccg cacaaatggg agggagtttc acatgagttg gcaggatcac atgaggaagg caacggctcc acgcgtttca aacgtcggca caaaagagaa aaatgtcacg cgtgtgaagt ttctccccga ctacgagcga tttggcatga aggagaagaa aatttcaaac gacatgaagc gtgtgctcta caagcgcatt atggaccttt ccgcaatgtt tccgaatatt caaataaccc tgaacggctc atcctttggc ttcaagtcct ttaaggacta tgcgactctg tacagtgcca tgaccccaaa gggagagaaa ccaccgccac catacgtata cgagagtaaa agcggttgcg ttgccttcat tccttcagta gtccccgggg tgcggcggat gtttggtgtg gtcaacggtg tggtaacgta taatggcggt acgcattgca atgctgcgca ggatatattg accggctgcc tcgatggcgt ggaacgggaa ttaaagaagg agaacaaagt gatggacact aatcgagtgc ttcgtcactt cactattcta gttttcctcg tgcaggtgca gccaaagttt gattctcaga acaaagctcg acttgtttct acccccacga tgccccgtgt tcctcggcaa gatgtgatga aatatcttct gcgcatgcct tttctcgagg ctcatgtgag tactattacg ggacagttag cgcaggaact aaataaggag atcggcaccg gacgccgtat gagtagcaaa accctcctga cctccataac gaaactggta gatgcaactt ctacacgccg tgacccaaaa catacccgca cgttaattgt tactgaaggt gactccgcaa aggctctcgc gcagaactct ttatcgagtg accaaaagcg atatacaggc gtatttccgc ttcggggtaa gctgctaaac gtgcgtaaca agaatcttaa gcgactgagg aactgcaagg agttgcagga actgttttgt gccctgggac ttgagctaga taaagattac accgacgccg atgaactacg ataccaacgc atacttatca tgacagatca ggacgcagat ggctcacaca ttaagggttt ggttatcaac gcgttcgagt ctttgtggcc ctcgttgctg gtacgaaatc ctgggttcat ctctatattc tccacaccca tcgtaaaggc acgactgcgc gacaagtcgg tggtatcctt cttcagcatg aaggagtttc acaagtggca gcgctcaaat gcaaatacac catacacatg taagtactat aagggtctcg gtacttctac cactgctgag ggaaaagagt acttcaagga tatggagaaa cacacaatgc gcttactcgt ggaccgctcc gatcataagc ttcttgacaa tgttttcgac tcacaggagg tagaatggcg aaaggactgg atgaccaagg cgaatgcttt taccggcgag gtggatattg atcgtagcaa gaaaatgcta acggtcacag attttgtgca taaggagatg gttcatttcg cccttgttgg taatgcccgt gcgcttgcgc actctgtaga cgggcttaag ccttctcagc gaaagattat ttgggctctt atgcggcggt ccggtaatga ggcggcgaag gtggcacaac tatcaggtta catatcagaa gcttccgctt ttcatcatgg tgagacttca ttgcaggaga cgatgattaa gatggcgcag agcttcactg gtggtaacaa cgtcaacctt ctcgtccctg agggtcagtt cggttctcgt cagcaactcg gtaatgatca tgcggcaccc cgttacattt tcacaaagct ttcaaaagta gcccgcttgc ttttccctag tgaggatgac ccattgctag actacattgt ggaagagggt cagcaggtgg agccgaacca ttacgttcca atcctaccgc tgctcctctg caacggaagt gtgggcatcg gtttcggttt tgcgtcgaat attccaccat tccacccgtt ggacgtatct gcagcggtac gagcgatgat tagcggcgaa gctgccaagt cggttgtccg tcgacttgtg ccgtgggctg taggctttca gggtgagata cgtcgtggcc ccgaaggaga gtttattgct gtgggaacgt atacttactg taagggtggt cgtgtgcatg ttacggagct tccttggacg tgtagcgttg aagcattccg tgagcacatt tcttacctcg ccacaaagga tattgttaac cgcattgccg actattccgg cgccaatcac gttgacattg atgtggaagt tgctcagggt gcggtgaaca catatgctga gtgcgagtcg gaacttggcc tcacgcaacg tattcacatc aacggtacag tcttttcacc gaatggaact ctttcacctc tggaaagtga cctcacaccc gtcctccagt ggcactacga ccgcagactt gatttatata aaaagaggcg acaacgtaat ttgatgctgt tggagcagga attggccaga gagaagtcga cactcaaatt tgtgcaacac ttccgtgccg gccacattga ctttgcgaat gctacggagg caacacttga aaaggtgtgt tcaaagttag ggttagtacg tgtagatgac tcgttcgact acattttgcg taaacccatc acgttctata ccaaaacaag ttttgaaaat cttctcaaga agatcgcgga gacggagcgg cgcattgaag ctctcaagaa gacaacccct gtgcagttgt ggttgggcga acttgaccaa tttgatcgct tctttcagga ccatgagaaa aagatggtgg aggctatttt gaaggaaaga aggcagcgat catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat