CENTER ID: | TrbrA.19530.a |
ORGANISM: | Trypanosoma brucei TREU927 |
ASSOCIATED DISEASE: | African trypanosomiasis (African Sleeping Sickness) |
CURRENT STATUS: | purified |
COMMUNITY REQUEST: | True |
NIH RISK GROUP: | 2 |
SELECT AGENT: | False |
NIH PRIORITY pathogens category: |
unclassified |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
TrbrA.19530.a.B1.GE41499 | full length | 1 | 839 | ||
TrbrA.19530.a.B2.GE41500 | C-term domain | 590 | 835 | ||
TrbrA.19530.a.B3.GE41501 | Tet-JBP oxygenase | 103 | 303 |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
TrbrA.19530.a.B1.PS38353 | full length | 1 | 839 |
METHOD | RESULTS |
---|---|
comparative modelling | Robetta_79414 |
RESOURCE | REFERENCE ID |
---|---|
EuPathDB: | TriTrypDB:Tb927.11.13640 |
UniProt: | P86938 |
HARVESTED ON: | 4/21/2017 |
SEQUENCED ON: | 5/3/2017 |
EXPECTED MW: | 96kDa |
OBSERVED MW: | 96kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | Low Expression |
SOLUBLE EXPRESSION LEVEL | Low Expression |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | data unavailable |
PERCENT COVERAGE: | data unavailable |
HARVESTED ON: | 4/21/2017 |
SEQUENCED ON: | 5/3/2017 |
EXPECTED MW: | 29kDa |
OBSERVED MW: | 30kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | Low Expression |
SOLUBLE EXPRESSION LEVEL | Insoluble |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 100 |
HARVESTED ON: | 4/21/2017 |
SEQUENCED ON: | 5/3/2017 |
EXPECTED MW: | 24kDa |
OBSERVED MW: | 24kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | Low Expression |
SOLUBLE EXPRESSION LEVEL | Insoluble |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 100 |
PURIFICATION DATe: | 9/14/2017 |
CONCENTRATION: | 4.68mg/ml |
OBSERVED MW: | 99kDa |
EXPRESSION LEVEL: | Low Expression |
PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
VIAL COUNT (approx.): | 8 |
VIAL VOLUME: | 110µl |
PERCENT IDENTITY: | data unavailable |
PERCENT COVERAGE: | data unavailable |