SSGCID: Target Page

TrbrA.19530.a

J-binding protein 1 (JBP1)

CENTER ID:	TrbrA.19530.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	AA START	AA STOP
TrbrA.19530.a.B1.GE41499	full length	1	839
TrbrA.19530.a.B2.GE41500	C-term domain	590	835
TrbrA.19530.a.B3.GE41501	Tet-JBP oxygenase	103	303

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.19530.a.B1.PS38353	full length		1	839

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_79414

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:Tb927.11.13640
UniProt:	P86938

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRRQVKKVLR EKADDSMKPG WDVYQPSNDV VYAFNHYMQG SQIDAEAREK AEKAFQEAVK KHPFHNNADH TVDFHGTTVF RNAKGKVCGV LIPKALPSFA TSMAADVLEC AVARTSLRSA LFGGVSPNSG IAGYFDYRGT PVELKCRKTS FTYEHTKEWR SVFPMIDYTS AIYKAALPDH WKAQDAAVPD VVRIHGSPFS TLTVNERFRT ASHTDNGDFD NGYGVLAVLK GEYSGLSLAL DDYGVCFNMQ PTDVLLFDTH LFHSNTELEA KEANATWNRL SCVFYYRAAL GEQPCVEEYR RRLKKAKEEK STSLSFNHIE QKDNGENTNK PAPVYPVSLT PFSCAASAWA LRGCAAAMLT RLHGLVRENA SLMTELFGEP VEVADGLPRR APEEIIPVHK HTNVQMHYLG GFSEKGDILN EAMNKRHYLD KENLQKMFGE EFVNIWTQSR THWLQLVKKE WEHQKETNPT RTRFSWNNTS AMNFAFFDLC DVAKQLMCGA FGDREVNKKE EQSFWGMFAA HLDNACINEI GMLQGSMGMH KLNVKLKDYN FGGTRYLKDM PPEEQERRRR RRLEIEQARR RAPICDSESG DWLRNEAFDY QTEDVAVNYE REQWITPENN AKRFGFPERG VYGAEGAATG TISVLIVLPK PTNHRQKTCE LPTSREADRI MKNPAAQRLL CAKPCNIGLS TSSNKSRTVL CGNIRIDKVF DGGSVGGKMY DFVIMRHLLA ATTGEREPLE CLVRWTSLAR YCTFVVEVDL LDRHHYILKS EIGEEYSAVS EICFSALYSA TYARDKVNLR TTPCLLSFID KSGNMLESRF KFNGSPLNTV AFVVRRREK

NT Sequence

atgcgaaggc aggtgaagaa ggtactgagg gagaaagcag atgactccat gaaacccggc tgggacgttt atcaaccgtc gaatgatgtg gtgtacgcct ttaaccacta catgcagggt tcccagattg atgccgaggc gagggagaag gccgaaaagg cctttcagga ggctgtgaaa aagcatccat ttcacaacaa cgccgaccat acagttgact tccatggtac cacagtgttc agaaacgcaa agggaaaggt ctgtggggtg ttaataccaa aggctctacc atcgttcgca acctctatgg cagcggacgt ccttgagtgc gcggtggcgc gtacctccct tcgttcggca ctgttcggtg gggtttcccc taatagtggc attgctggtt atttcgacta ccgtggtacc cccgtggaac tgaagtgtcg caagacctcc tttacctacg agcacactaa agagtggcga agtgtgttcc ctatgattga ctatacgagc gcaatttata aggctgctct tccagaccac tggaaagcac aggatgcagc ggtgccggac gtagtacgca tccacggttc tcctttttcg acactgacag tcaatgagcg gtttcgcacc gcctcacaca cagacaatgg cgacttcgat aacggttatg gtgtcctagc tgtgctcaaa ggtgagtata gtggtcttag cctagccctc gatgactatg gtgtttgctt caacatgcag ccaacagatg tcttactctt cgacacgcac ctgttccact caaacacgga gctagaggcc aaggaggcca acgctacctg gaatcggttg tcgtgtgtgt tctactatag ggcggctctg ggggagcaac cttgcgtgga ggaataccgg aggcgcctta agaaggctaa ggaagaaaaa tccacgtcgc tctctttcaa ccatattgag cagaaggaca acggagaaaa caccaataag cctgccccgg tatatccggt ttccttgaca ccgtttagtt gtgcagcaag tgcatgggcc ctacgtggct gtgcagctgc gatgctcacc aggttgcacg gcttggtgcg agaaaatgca tcgctgatga cggaactgtt tggtgagccg gtggaagtag ctgatggact accgcggcgc gcaccggaag aaattatacc agtccacaag cacaccaatg tgcaaatgca ttatttgggt gggttcagcg agaagggcga tattttgaac gaagctatga ataagcggca ttatcttgac aaggaaaatc tccaaaaaat gtttggagaa gagttcgtta acatatggac tcagagtcga acacactggc tacaactcgt gaaaaaggaa tgggaacatc aaaaggagac caacccgacc cgcacaagat tcagttggaa caatacgtct gccatgaact ttgccttttt tgacttgtgc gatgtagcga agcagctgat gtgtggagca tttggtgacc gtgaagtaaa caagaaggaa gagcaaagct tctggggaat gtttgccgcc cacctggata atgcatgcat aaatgaaatt ggtatgctcc aaggctccat gggcatgcac aaacttaacg tcaagttgaa ggattacaac ttcgggggga cacgctacct caaggacatg ccaccggagg agcaggaaag gaggcgaagg aggcgcttgg agatcgagca agcgcggagg cgtgcaccca tctgtgacag tgagtcgggc gattggctac gtaacgaggc cttcgattac cagacggaag atgtagcggt taactatgaa cgggagcagt ggataacacc agaaaataat gcaaaacgct tcggtttccc ggaacgcggg gtctatggtg ctgaaggtgc tgccacgggt accattagcg ttcttattgt tcttccaaag cccaccaacc atcggcagaa aacgtgtgaa ctgccaacgt cgcgggaagc agatcgaatt atgaaaaacc ctgctgcaca acggctgttg tgcgcaaagc cttgtaacat agggctctct accagcagta ataagtcacg caccgtgctt tgcggtaata tccggattga taaagtcttt gatggtggta gcgttggtgg aaaaatgtac gatttcgtga tcatgcgtca ccttcttgcc gcaaccaccg gagagcgtga acccctggaa tgcctagtgc gttggacatc ccttgctcga tactgtacct tcgttgtcga ggttgacttg ttagacagac accattacat cctcaagagc gaaatagggg aggagtacag tgcggtttcg gagatatgct ttagcgcgct ctactccgca acctatgcac gagacaaggt caatctacgt actacccctt gtcttttgtc cttcatcgat aaaagcggaa acatgttgga gtctcgattc aagttcaatg gatcgccctt gaatacggtg gcctttgtcg tccgcagacg ggagaaatag

Details for TrbrA.19530.a.B1.GE41499

HARVESTED ON:	4/21/2017
SEQUENCED ON:	5/3/2017
EXPECTED MW:	96kDa
OBSERVED MW:	96kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMR RQVKKVLREK ADDSMKPGWD VYQPSNDVVY AFNHYMQGSQ IDAEAREKAE KAFQEAVKKH PFHNNADHTV DFHGTTVFRN AKGKVCGVLI PKALPSFATS MAADVLECAV ARTSLRSALF GGVSPNSGIA GYFDYRGTPV ELKCRKTSFT YEHTKEWRSV FPMIDYTSAI YKAALPDHWK AQDAAVPDVV RIHGSPFSTL TVNERFRTAS HTDNGDFDNG YGVLAVLKGE YSGLSLALDD YGVCFNMQPT DVLLFDTHLF HSNTELEAKE ANATWNRLSC VFYYRAALGE QPCVEEYRRR LKKAKEEKST SLSFNHIEQK DNGENTNKPA PVYPVSLTPF SCAASAWALR GCAAAMLTRL HGLVRENASL MTELFGEPVE VADGLPRRAP EEIIPVHKHT NVQMHYLGGF SEKGDILNEA MNKRHYLDKE NLQKMFGEEF VNIWTQSRTH WLQLVKKEWE HQKETNPTRT RFSWNNTSAM NFAFFDLCDV AKQLMCGAFG DREVNKKEEQ SFWGMFAAHL DNACINEIGM LQGSMGMHKL NVKLKDYNFG GTRYLKDMPP EEQERRRRRR LEIEQARRRA PICDSESGDW LRNEAFDYQT EDVAVNYERE QWITPENNAK RFGFPERGVY GAEGAATGTI SVLIVLPKPT NHRQKTCELP TSREADRIMK NPAAQRLLCA KPCNIGLSTS SNKSRTVLCG NIRIDKVFDG GSVGGKMYDF VIMRHLLAAT TGEREPLECL VRWTSLARYC TFVVEVDLLD RHHYILKSEI GEEYSAVSEI CFSALYSATY ARDKVNLRTT PCLLSFIDKS GNMLESRFKF NGSPLNTVAF VVRRREK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgaa ggcaggtgaa gaaggtactg agggagaaag cagatgactc catgaaaccc ggctgggacg tttatcaacc gtcgaatgat gtggtgtacg cctttaacca ctacatgcag ggttcccaga ttgatgccga ggcgagggag aaggccgaaa aggcctttca ggaggctgtg aaaaagcatc catttcacaa caacgccgac catacagttg acttccatgg taccacagtg ttcagaaacg caaagggaaa ggtctgtggg gtgttaatac caaaggctct accatcgttc gcaacctcta tggcagcgga cgtccttgag tgcgcggtgg cgcgtacctc ccttcgttcg gcactgttcg gtggggtttc ccctaatagt ggcattgctg gttatttcga ctaccgtggt acccccgtgg aactgaagtg tcgcaagacc tcctttacct acgagcacac taaagagtgg cgaagtgtgt tccctatgat tgactatacg agcgcaattt ataaggctgc tcttccagac cactggaaag cacaggatgc agcggtgccg gacgtagtac gcatccacgg ttctcctttt tcgacactga cagtcaatga gcggtttcgc accgcctcac acacagacaa tggcgacttc gataacggtt atggtgtcct agctgtgctc aaaggtgagt atagtggtct tagcctagcc ctcgatgact atggtgtttg cttcaacatg cagccaacag atgtcttact cttcgacacg cacctgttcc actcaaacac ggagctagag gccaaggagg ccaacgctac ctggaatcgg ttgtcgtgtg tgttctacta tagggcggct ctgggggagc aaccttgcgt ggaggaatac cggaggcgcc ttaagaaggc taaggaagaa aaatccacgt cgctctcttt caaccatatt gagcagaagg acaacggaga aaacaccaat aagcctgccc cggtatatcc ggtttccttg acaccgttta gttgtgcagc aagtgcatgg gccctacgtg gctgtgcagc tgcgatgctc accaggttgc acggcttggt gcgagaaaat gcatcgctga tgacggaact gtttggtgag ccggtggaag tagctgatgg actaccgcgg cgcgcaccgg aagaaattat accagtccac aagcacacca atgtgcaaat gcattatttg ggtgggttca gcgagaaggg cgatattttg aacgaagcta tgaataagcg gcattatctt gacaaggaaa atctccaaaa aatgtttgga gaagagttcg ttaacatatg gactcagagt cgaacacact ggctacaact cgtgaaaaag gaatgggaac atcaaaagga gaccaacccg acccgcacaa gattcagttg gaacaatacg tctgccatga actttgcctt ttttgacttg tgcgatgtag cgaagcagct gatgtgtgga gcatttggtg accgtgaagt aaacaagaag gaagagcaaa gcttctgggg aatgtttgcc gcccacctgg ataatgcatg cataaatgaa attggtatgc tccaaggctc catgggcatg cacaaactta acgtcaagtt gaaggattac aacttcgggg ggacacgcta cctcaaggac atgccaccgg aggagcagga aaggaggcga aggaggcgct tggagatcga gcaagcgcgg aggcgtgcac ccatctgtga cagtgagtcg ggcgattggc tacgtaacga ggccttcgat taccagacgg aagatgtagc ggttaactat gaacgggagc agtggataac accagaaaat aatgcaaaac gcttcggttt cccggaacgc ggggtctatg gtgctgaagg tgctgccacg ggtaccatta gcgttcttat tgttcttcca aagcccacca accatcggca gaaaacgtgt gaactgccaa cgtcgcggga agcagatcga attatgaaaa accctgctgc acaacggctg ttgtgcgcaa agccttgtaa catagggctc tctaccagca gtaataagtc acgcaccgtg ctttgcggta atatccggat tgataaagtc tttgatggtg gtagcgttgg tggaaaaatg tacgatttcg tgatcatgcg tcaccttctt gccgcaacca ccggagagcg tgaacccctg gaatgcctag tgcgttggac atcccttgct cgatactgta ccttcgttgt cgaggttgac ttgttagaca gacaccatta catcctcaag agcgaaatag gggaggagta cagtgcggtt tcggagatat gctttagcgc gctctactcc gcaacctatg cacgagacaa ggtcaatcta cgtactaccc cttgtctttt gtccttcatc gataaaagcg gaaacatgtt ggagtctcga ttcaagttca atggatcgcc cttgaatacg gtggcctttg tcgtccgcag acgggagaaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for TrbrA.19530.a.B2.GE41500

HARVESTED ON:	4/21/2017
SEQUENCED ON:	5/3/2017
EXPECTED MW:	29kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG DWLRNEAFDY QTEDVAVNYE REQWITPENN AKRFGFPERG VYGAEGAATG TISVLIVLPK PTNHRQKTCE LPTSREADRI MKNPAAQRLL CAKPCNIGLS TSSNKSRTVL CGNIRIDKVF DGGSVGGKMY DFVIMRHLLA ATTGEREPLE CLVRWTSLAR YCTFVVEVDL LDRHHYILKS EIGEEYSAVS EICFSALYSA TYARDKVNLR TTPCLLSFID KSGNMLESRF KFNGSPLNTV AFVVR

Validated NT Sequence

atggctcacc accaccacca ccatatgggc gattggctac gtaacgaggc cttcgattac cagacggaag atgtagcggt taactatgaa cgggagcagt ggataacacc agaaaataat gcaaaacgct tcggtttccc ggaacgcggg gtctatggtg ctgaaggtgc tgccacgggt accattagcg ttcttattgt tcttccaaag cccaccaacc atcggcagaa aacgtgtgaa ctgccaacgt cgcgggaagc agatcgaatt atgaaaaacc ctgctgcaca acggctgttg tgcgcaaagc cttgtaacat agggctctct accagcagta ataagtcacg caccgtgctt tgcggtaata tccgaattga taaagtcttt gatggtggta gcgttggtgg aaaaatgtac gatttcgtga tcatgcgtca ccttcttgcc gcaaccaccg gagagcgtga acccctggaa tgcctagtgc gttggacatc ccttgctcga tactgtacct tcgttgtcga ggttgacttg ttagacagac atcattacat cctcaagagc gaaatagggg aggagtacag tgcggtttcg gagatatgct ttagcgcgct ctactccgca acctatgcac gagacaaggt caatctgcgc actacccctt gtcttttgtc cttcatcgat aaaagcggaa acatgttgga gtctcgattc aagttcaatg gatcgccctt gaatacggtg gcctttgtcg tccgctaagt gagtaagat

Expected Protein Sequence

MAHHHHHHGD WLRNEAFDYQ TEDVAVNYER EQWITPENNA KRFGFPERGV YGAEGAATGT ISVLIVLPKP TNHRQKTCEL PTSREADRIM KNPAAQRLLC AKPCNIGLST SSNKSRTVLC GNIRIDKVFD GGSVGGKMYD FVIMRHLLAA TTGEREPLEC LVRWTSLARY CTFVVEVDLL DRHHYILKSE IGEEYSAVSE ICFSALYSAT YARDKVNLRT TPCLLSFIDK SGNMLESRFK FNGSPLNTVA FVVR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggcgatt ggctacgtaa cgaggccttc gattaccaga cggaagatgt agcggttaac tatgaacggg agcagtggat aacaccagaa aataatgcaa aacgcttcgg tttcccggaa cgcggggtct atggtgctga aggtgctgcc acgggtacca ttagcgttct tattgttctt ccaaagccca ccaaccatcg gcagaaaacg tgtgaactgc caacgtcgcg ggaagcagat cgaattatga aaaaccctgc tgcacaacgg ctgttgtgcg caaagccttg taacataggg ctctctacca gcagtaataa gtcacgcacc gtgctttgcg gtaatatccg gattgataaa gtctttgatg gtggtagcgt tggtggaaaa atgtacgatt tcgtgatcat gcgtcacctt cttgccgcaa ccaccggaga gcgtgaaccc ctggaatgcc tagtgcgttg gacatccctt gctcgatact gtaccttcgt tgtcgaggtt gacttgttag acagacacca ttacatcctc aagagcgaaa taggggagga gtacagtgcg gtttcggaga tatgctttag cgcgctctac tccgcaacct atgcacgaga caaggtcaat ctacgtacta ccccttgtct tttgtccttc atcgataaaa gcggaaacat gttggagtct cgattcaagt tcaatggatc gcccttgaat acggtggcct ttgtcgtccg ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for TrbrA.19530.a.B3.GE41501

HARVESTED ON:	4/21/2017
SEQUENCED ON:	5/3/2017
EXPECTED MW:	24kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA ADVLECAVAR TSLRSALFGG VSPNSGIAGY FDYRGTPVEL KCRKTSFTYE HTKEWRSVFP MIDYTSAIYK AALPDHWKAQ DAAVPDVVRI HGSPFSTLTV NERFRTASHT DNGDFDNGYG VLAVLKGEYS GLSLALDDYG VCFNMQPTDV LLFDTHLFHS NTELEAKEAN ATWNRLSCVF YYRAALGEQP CVEEYRRRL

Validated NT Sequence

atggctcacc accaccacca ccatatggca gcggacgtcc ttgagtgcgc ggtggcgcgt acctcccttc gttcggcact gttcggtggg gtttccccta atagtggcat tgctggttat ttcgactacc gtggtacccc cgtggaactg aagtgtcgca agacctcctt cacctacgag cacactaaag agtggcggag tgtgttccct atgattgact atacgagcgc aatttataag gctgctcttc cagaccactg gaaagcacag gatgcagcgg tgccggacgt agtacgcatc cacggttctc ctttttcgac actgacagtc aatgagcggt ttcgcaccgc ctcacacaca gacaatggcg acttcgataa cggttatggt gtcctagctg tgctcaaagg tgagtatagt ggtcttagcc tagccctcga tgactatggt gtttgcttca acatgcagcc aacagatgtc ttactcttcg atacgcacct gttccactca aacacggagc tagaggccaa ggaggccaac gctacctgga atcggttgtc gtgtgtgttc tactataggg cggctctggg ggagcaacct tgcgtggagg aataccggag gcgcctttaa gtgagtaaga t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag cggacgtcct tgagtgcgcg gtggcgcgta cctcccttcg ttcggcactg ttcggtgggg tttcccctaa tagtggcatt gctggttatt tcgactaccg tggtaccccc gtggaactga agtgtcgcaa gacctccttt acctacgagc acactaaaga gtggcgaagt gtgttcccta tgattgacta tacgagcgca atttataagg ctgctcttcc agaccactgg aaagcacagg atgcagcggt gccggacgta gtacgcatcc acggttctcc tttttcgaca ctgacagtca atgagcggtt tcgcaccgcc tcacacacag acaatggcga cttcgataac ggttatggtg tcctagctgt gctcaaaggt gagtatagtg gtcttagcct agccctcgat gactatggtg tttgcttcaa catgcagcca acagatgtct tactcttcga cacgcacctg ttccactcaa acacggagct agaggccaag gaggccaacg ctacctggaa tcggttgtcg tgtgtgttct actatagggc ggctctgggg gagcaacctt gcgtggagga ataccggagg cgcctttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for TrbrA.19530.a.B1.PS38353

PURIFICATION DATe:	9/14/2017
CONCENTRATION:	4.68mg/ml
OBSERVED MW:	99kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	8
VIAL VOLUME:	110µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGAA GGCAGGTGAA GAAGGTACTG AGGGAGAAAG CAGATGACTC CATGAAACCC GGCTGGGACG TTTATCAACC GTCGAATGAT GTGGTGTACG CCTTTAACCA CTACATGCAG GGTTCCCAGA TTGATGCCGA GGCGAGGGAG AAGGCCGAAA AGGCCTTTCA GGAGGCTGTG AAAAAGCATC CATTTCACAA CAACGCCGAC CATACAGTTG ACTTCCATGG TACCACAGTG TTCAGAAACG CAAAGGGAAA GGTCTGTGGG GTGTTAATAC CAAAGGCTCT ACCATCGTTC GCAACCTCTA TGGCAGCGGA CGTCCTTGAG TGCGCGGTGG CGCGTACCTC CCTTCGTTCG GCACTGTTCG GTGGGGTTTC CCCTAATAGT GGCATTGCTG GTTATTTCGA CTACCGTGGT ACCCCCGTGG AACTGAAGTG TCGCAAGACC TCCTTTACCT ACGAGCACAC TAAAGAGTGG CGAAGTGTGT TCCCTATGAT TGACTATACG AGCGCAATTT ATAAGGCTGC TCTTCCAGAC CACTGGAAAG CACAGGATGC AGCGGTGCCG GACGTAGTAC GCATCCACGG TTCTCCTTTT TCGACACTGA CAGTCAATGA GCGGTTTCGC ACCGCCTCAC ACACAGACAA TGGCGACTTC GATAACGGTT ATGGTGTCCT AGCTGTGCTC AAAGGTGAGT ATAGTGGTCT TAGCCTAGCC CTCGATGACT ATGGTGTTTG CTTCAACATG CAGCCAACAG ATGTCTTACT CTTCGACACG CACCTGTTCC ACTCAAACAC GGAGCTAGAG GCCAAGGAGG CCAACGCTAC CTGGAATCGG TTGTCGTGTG TGTTCTACTA TAGGGCGGCT CTGGGGGAGC AACCTTGCGT GGAGGAATAC CGGAGGCGCC TTAAGAAGGC TAAGGAAGAA AAATCCACGT CGCTCTCTTT CAACCATATT GAGCAGAAGG ACAACGGAGA AAACACCAAT AAGCCTGCCC CGGTATATCC GGTTTCCTTG ACACCGTTTA GTTGTGCAGC AAGTGCATGG GCCCTACGTG GCTGTGCAGC TGCGATGCTC ACCAGGTTGC ACGGCTTGGT GCGAGAAAAT GCATCGCTGA TGACGGAACT GTTTGGTGAG CCGGTGGAAG TAGCTGATGG ACTACCGCGG CGCGCACCGG AAGAAATTAT ACCAGTCCAC AAGCACACCA ATGTGCAAAT GCATTATTTG GGTGGGTTCA GCGAGAAGGG CGATATTTTG AACGAAGCTA TGAATAAGCG GCATTATCTT GACAAGGAAA ATCTCCAAAA AATGTTTGGA GAAGAGTTCG TTAACATATG GACTCAGAGT CGAACACACT GGCTACAACT CGTGAAAAAG GAATGGGAAC ATCAAAAGGA GACCAACCCG ACCCGCACAA GATTCAGTTG GAACAATACG TCTGCCATGA ACTTTGCCTT TTTTGACTTG TGCGATGTAG CGAAGCAGCT GATGTGTGGA GCATTTGGTG ACCGTGAAGT AAACAAGAAG GAAGAGCAAA GCTTCTGGGG AATGTTTGCC GCCCACCTGG ATAATGCATG CATAAATGAA ATTGGTATGC TCCAAGGCTC CATGGGCATG CACAAACTTA ACGTCAAGTT GAAGGATTAC AACTTCGGGG GGACACGCTA CCTCAAGGAC ATGCCACCGG AGGAGCAGGA AAGGAGGCGA AGGAGGCGCT TGGAGATCGA GCAAGCGCGG AGGCGTGCAC CCATCTGTGA CAGTGAGTCG GGCGATTGGC TACGTAACGA GGCCTTCGAT TACCAGACGG AAGATGTAGC GGTTAACTAT GAACGGGAGC AGTGGATAAC ACCAGAAAAT AATGCAAAAC GCTTCGGTTT CCCGGAACGC GGGGTCTATG GTGCTGAAGG TGCTGCCACG GGTACCATTA GCGTTCTTAT TGTTCTTCCA AAGCCCACCA ACCATCGGCA GAAAACGTGT GAACTGCCAA CGTCGCGGGA AGCAGATCGA ATTATGAAAA ACCCTGCTGC ACAACGGCTG TTGTGCGCAA AGCCTTGTAA CATAGGGCTC TCTACCAGCA GTAATAAGTC ACGCACCGTG CTTTGCGGTA ATATCCGGAT TGATAAAGTC TTTGATGGTG GTAGCGTTGG TGGAAAAATG TACGATTTCG TGATCATGCG TCACCTTCTT GCCGCAACCA CCGGAGAGCG TGAACCCCTG GAATGCCTAG TGCGTTGGAC ATCCCTTGCT CGATACTGTA CCTTCGTTGT CGAGGTTGAC TTGTTAGACA GACACCATTA CATCCTCAAG AGCGAAATAG GGGAGGAGTA CAGTGCGGTT TCGGAGATAT GCTTTAGCGC GCTCTACTCC GCAACCTATG CACGAGACAA GGTCAATCTA CGTACTACCC CTTGTCTTTT GTCCTTCATC GATAAAAGCG GAAACATGTT GGAGTCTCGA TTCAAGTTCA ATGGATCGCC CTTGAATACG GTGGCCTTTG TCGTCCGCAG ACGGGAGAAA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT