SSGCID: Target Page

TrbrA.19529.a

J2IP1

CENTER ID:	TrbrA.19529.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.19529.a.B1.GE41497	full length		1	238

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:Tb927.10.3070
UniProt:	Q38BT9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDAADATGGG SVKPLAELIQ LIRVRGGVTK ECARPRRTND HSNDLCAVPF SLPVWKRARG HSPPRAALPV KESVSRRPEL STTVRRRRRR MEGCHAVTTR NVSTCSADFQ IMEKLSVGAY HLTYVHVDLY HHGLKRLMDD AVLRTNPKKI VGRKCCYLKP GTDLVVTGCV IEFTKNDLSV QVAPDKGIDV DVEWVKLEDV YVPENIDKVR VRVSDEVRSG LQKVKEAPKE MRRRFASQ

NT Sequence

atggacgccg cagatgccac cggagggggc agcgtaaagc cattggctga attaattcag ctaatccgtg tccgcggtgg cgttacaaag gagtgtgcac gccctaggcg tactaacgat cattcgaatg acctttgcgc tgtacccttt tcgttacctg tgtggaaaag ggcgcgaggt cacagtcccc caagggccgc tttaccagtg aaggagtcgg tgtcaaggcg acctgaactg tcaacaacag tgagaagacg acgacgacga atggaaggtt gccatgcggt gactacgaga aatgtttcta cctgttcggc tgactttcag attatggaaa aattatcagt tggggcgtat cacttgacgt acgtgcacgt cgatctttat catcacgggt tgaaacgtct gatggacgat gcggtgctta ggacaaaccc gaagaaaatc gttggacgca agtgctgcta tttaaaaccc ggcacagatc ttgtggtgac tggttgcgta attgaattca ctaagaatga tctatctgtg caagtggccc cggataaggg cattgacgtg gacgtggagt gggtgaagtt ggaggatgtt tacgtcccgg aaaacattga taaagtgaga gtacgtgttt ccgatgaagt gcgctcggga ttacagaaag taaaagaggc tccaaaagag atgaggagaa ggtttgcttc acagtaa

Details for TrbrA.19529.a.B1.GE41497

HARVESTED ON:	4/21/2017
SEQUENCED ON:	5/3/2017
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMD AADATGGGSV KPLAELIQLI RVRGGVTKEC TRPRRTNDHS NDLCAVPFSL PVWKRARGHS PPRAALPVKE SVSTRPELST TVRRRRRRME GCHAVTTRNV STCSADFQIM EKLSVGAYHL TYVHVDLYHH GLKRLMDDAV LRTNPKKIVG RKCCYLKPGT DLVVTGCVIE FTKNDLSVQV APDKGIDVDV EWVKLEDVYV PENIDKVRVR VSDEVRSGLQ KVKEAPKEMR RRFASQ

Validated NT Sequence

atggctcacc accaccacca ccatatggac gccgcagatg ccaccggagg gggcagcgta aagccactgg ctgaattaat tcagctaatc cgtgtccgcg gtggcgttac aaaggagtgt acacgcccta ggcgtactaa cgatcattcg aatgaccttt gcgctgtacc cttttcgtta cctgtgtgga aaagggcgcg aggtcacagt cccccaaggg ccgctttacc agtgaaggag tcggtgtcaa cgcgacctga actgtcaaca acagtgagaa gacgacgacg acgaatggaa ggttgccatg cggtgactac gagaaatgtt tctacctgct cagctgactt tcagattatg gaaaaattgt cagttggggc gtatcacttg acgtacgtgc acgtcgatct ttatcatcac gggttgaaac gtctgatgga cgatgcggtg cttaggacaa acccgaagaa aatcgttgga cgcaagtgct gctatttaaa acccggcaca gatcttgtgg tgactggttg cgtaattgaa ttcactaaga atgatctatc tgtgcaagtg gccccggata agggcattga cgtggacgtg gagtgggtga agttggagga tgtttacgtc ccggaaaaca ttgataaagt gagagtacgt gtttccgatg aagtgcgctc gggattacag aaagtaaaag aggctccaaa agagatgagg agaaggtttg cttcacagta agtgagtaag at

Expected Protein Sequence

MAHHHHHHMD AADATGGGSV KPLAELIQLI RVRGGVTKEC ARPRRTNDHS NDLCAVPFSL PVWKRARGHS PPRAALPVKE SVSRRPELST TVRRRRRRME GCHAVTTRNV STCSADFQIM EKLSVGAYHL TYVHVDLYHH GLKRLMDDAV LRTNPKKIVG RKCCYLKPGT DLVVTGCVIE FTKNDLSVQV APDKGIDVDV EWVKLEDVYV PENIDKVRVR VSDEVRSGLQ KVKEAPKEMR RRFASQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggacg ccgcagatgc caccggaggg ggcagcgtaa agccattggc tgaattaatt cagctaatcc gtgtccgcgg tggcgttaca aaggagtgtg cacgccctag gcgtactaac gatcattcga atgacctttg cgctgtaccc ttttcgttac ctgtgtggaa aagggcgcga ggtcacagtc ccccaagggc cgctttacca gtgaaggagt cggtgtcaag gcgacctgaa ctgtcaacaa cagtgagaag acgacgacga cgaatggaag gttgccatgc ggtgactacg agaaatgttt ctacctgttc ggctgacttt cagattatgg aaaaattatc agttggggcg tatcacttga cgtacgtgca cgtcgatctt tatcatcacg ggttgaaacg tctgatggac gatgcggtgc ttaggacaaa cccgaagaaa atcgttggac gcaagtgctg ctatttaaaa cccggcacag atcttgtggt gactggttgc gtaattgaat tcactaagaa tgatctatct gtgcaagtgg ccccggataa gggcattgac gtggacgtgg agtgggtgaa gttggaggat gtttacgtcc cggaaaacat tgataaagtg agagtacgtg tttccgatga agtgcgctcg ggattacaga aagtaaaaga ggctccaaaa gagatgagga gaaggtttgc ttcacagtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at