TrbrA.19528.a

J-binding protein 2 (JBP2)

CENTER ID:	TrbrA.19528.a
ORGANISM:	Trypanosoma brucei TREU927
ASSOCIATED DISEASE:	African trypanosomiasis (African Sleeping Sickness)
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.19528.a.B1.GE41493	full length		1	1077
TrbrA.19528.a.B3.GE41495	Tet_JBP oxygenase		223	476

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrbrA.19528.a.B3.PS38335	Tet_JBP oxygenase		223	476

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_79431

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:Tb927.7.4650
UniProt:	Q57X81

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPMFMDGASQ VLQQVLQTVL VTSEPAIVIP GSFLGELDVI VDEAKNHGMK LVSIPKGGIT ILPPIPMSES SLTRLCKDYY GLKTDAERLA LFSNLEETFP TAPGVSLPCR LLYHPRDYIC RIVHLCAELV TASDEEYQKA YDIVPLLHIR PVQNVCEELR RQFRAGALTQ RLPLGQRVDV QFKRTVVHLD GSMDPFPRNA AEAAVNIAPV ALDAVDDIYE GFDVTGTEVV DIPTGKVSEY LSEKDFELVT EDSVLLDPTG KRVQAIFIRG GIDKDICRRA AADVEGVATK QNMRRLTNGG VRNPDTGILG YYDYLNNPTK RKCRMTEFTR RNWGKIIGPC GELLQLLDQL YKENAPDHYE LQRRVIPPEY MLFNTVFSTV SVNKNFRTAV HRDKGDFRGG LTALCVLDGN YEGCYLALKS ARKAFCLQVG DVLFFDSSLE HGNTEVHNRE GSWRRISIVC YLRCGLMSHT CETERSMRLR NQIMSDRLHA DSADSVVNLN GVTGHLPPLC IPFKIAKTLS LTQHAALRFV SRRIKEGDGC VLALTMGLGK TLVSLTICYS YIYNNGPCDI LIVAPKTLLQ HWMQEAKKWK DYGLVFPGFI VLNNVDSSSF EDDLSNYEQQ GTTTNPKKSY VFVINPGYIK SFLSRVKGFR PALIVVDEGH CISSKESKLR EVLDSLYCSA RVVLTGTPVQ NNAEELYRLV GWVDDKVHST LPQRDFNEFS NSINRYVNGD DSAFCDALFA QRYIHEWMSP YVFTVMKVDL PPLHDYIIIC NFSAVQQKMF EERIKVDATD NLLCLKASEH RPYHLSTHPL CFLGFLTGIW RTGQVDIEEE PGEFEELGTY RLSRDDDALA KDCSSLLENG KLADFVALSG KLTALISILH SIFEKMEKAV IFSQYIGSQD FIARTLTAYK ISVVTIRGKD CQQRRRRVVE MFRDDKNVLC LVVSTQIGAY GLDLTAANHV ILWDTWWNPQ VESQAIARCY RQNQSKAVIA YKLASGFEDA TVLKAQARKR ALFKCLINEE TSQVVPGHDL VDYTSSEEDD DRRHLWETLK TCTLEGGKPA VTKIIRNIDT VKSERWI

NT Sequence

atgcctatgt ttatggatgg ggcgtctcaa gtgctgcagc aggtgctaca aactgtctta gtaacgagtg agccagcgat cgttataccc ggttccttct tgggtgaact tgatgttatc gttgacgagg ctaaaaatca tggaatgaag ttagtgagta tcccgaaagg tggcatcacg attctaccac caataccgat gtcagaatct tctcttacca ggctatgcaa agactactac gggttaaaga ctgatgcaga gcgtttggcc ctgttcagca acctggagga gacattcccg acggctccag gtgtatcctt accgtgccgc cttctttacc accctcgcga ttacatatgt cgaattgtcc acctctgtgc ggaactggtt accgcttctg atgaggaata tcaaaaagcg tacgatattg ttccgcttct gcacatcaga cctgtgcaaa atgtatgtga agaacttcgc cgtcagttcc gagctggtgc tctcacacag aggcttcctc tcggacagcg cgtggacgtt caattcaaga ggactgtggt ccatctagac ggttcgatgg atccattccc tcgcaatgct gctgaagcag ctgtaaacat tgcgcctgtt gctctggatg cagtggatga tatttatgaa ggctttgacg ttaccggtac cgaggtggtt gacatcccta caggcaaggt ttcagagtac ctctctgaaa aggattttga gctggtgacc gaggatagtg ttttgctgga cccaaccgga aagcgtgtgc aggccatctt tatacgtggt ggcattgata aagatatctg tcgacgtgcc gcagctgatg tggagggagt ggctaccaaa caaaatatgc ggagattgac aaatggtggt gtgagaaatc cggacacggg tatccttggc tattacgact atctcaacaa tccaacgaag cgtaagtgcc gcatgactga gttcacacgc aggaactggg gtaagattat cggaccgtgt ggtgagttac tgcagttgtt agatcaacta tacaaggaaa acgccccaga ccattacgaa cttcagcgca gggtcatacc gcctgagtac atgctattca acaccgtttt cagtactgta agtgtgaaca agaactttcg aaccgccgtt catcgagaca aaggtgactt tcgcggtggt cttactgcac tctgcgtact tgacggtaat tacgaggggt gctacctagc cttaaaaagt gcgcgcaagg ccttctgtct tcaggttggt gatgtcttat ttttcgacag ttctctggaa cacggaaaca cggaagtaca caatcgtgaa ggaagttgga gacgaatcag cattgtatgc tatctgcggt gtgggttgat gtcccacaca tgtgaaacgg agcggagcat gagactccgg aaccagatca tgagcgatcg tctgcatgct gacagcgcag acagcgtggt caatttgaac ggagtcaccg gccaccttcc acctttgtgt attcctttca aaatagcaaa gaccctgagc ttaactcagc atgcggccct tagatttgtt tcacgtcgta tcaaggaagg agacggttgt gtgctggcgt taacaatggg acttgggaag acactggttt cactgacgat ctgctattct tatatttaca acaatgggcc gtgtgacatt ctgatcgttg cgccgaagac actgcttcaa cactggatgc aggaggcgaa aaagtggaag gactatggtc tcgtattccc ggggtttatt gtactaaaca acgtggactc cagctccttc gaggatgatc tcagtaacta tgaacaacaa ggcacaacta ctaaccctaa gaagagttac gtctttgtca tcaatcctgg ctacatcaaa tcatttcttt cgagggtaaa ggggttccgc cccgctctca tagtcgttga tgaaggccat tgcatatcgt cgaaggaaag taaactgagg gaggtactgg atagtttgta ttgctcggca agggtagtac tcactgggac gccggtgcag aataacgctg aggaactcta ccgtcttgtt gggtgggtgg atgacaaggt tcattccaca ttgccgcaac gcgattttaa cgagttttca aactctatca accgctatgt taacggtgat gatagtgctt tttgtgatgc tctcttcgca caacgatata tccacgagtg gatgagcccc tatgtcttta ccgttatgaa ggttgatctt ccaccgctgc acgactacat tattatatgc aacttttccg ctgtgcagca gaaaatgttc gaggagcgca ttaaagtcga tgcaacagat aacctattgt gtctgaaggc gtccgaacat cggccatacc acctttcaac acatccactg tgtttcttgg gttttttgac cggtatatgg agaacaggtc aagttgatat cgaagaagaa ccaggggagt tcgaagagtt gggaacgtac aggcttagcc gtgatgatga cgctttagcg aaggattgtt caagtttgct tgaaaacggg aaactggccg actttgttgc gctcagtggt aagctaacag ccctcatttc gattttacac agcattttcg agaagatgga aaaggctgtg atattttcac aatacattgg gtcgcaagac ttcattgcaa gaaccttgac ggcttataag attagtgtag ttacaattcg tggaaaagat tgccagcaac gtcgacgaag ggttgttgag atgttcaggg atgataagaa cgtactctgc ttggttgtgt ccactcagat tggcgcgtac ggtcttgatt taactgcggc gaatcacgtt attctgtggg acacgtggtg gaatcctcag gttgagtcac aggcaattgc gcggtgctac agacaaaatc agtcaaaggc tgttattgcc tataaattag catcgggatt cgaagacgcc acagttttaa aagcccaagc aagaaaaagg gcactgttca agtgcctcat aaacgaggaa acctcacagg tggttcctgg gcacgatttg gttgactaca caagcagcga agaagatgat gataggcgtc atctatggga gaccttaaaa acgtgtaccc ttgagggggg aaaacctgct gtaacaaaga tcattcgcaa tattgatact gtaaagagcg agcggtggat ataa

Details for TrbrA.19528.a.B1.GE41493

HARVESTED ON:	4/21/2017
SEQUENCED ON:	5/3/2017
EXPECTED MW:	122kDa
OBSERVED MW:	122kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMP MFMDGASQVL QQVLQTVLVT SEPAIVIPGS FLGELDVIVD EAKNHGMKLV SIPKGGITIL PPIPMSESSL TRLCKDYYGL KTDAERLALF SNLEETFPTA PGVSLPCRLL YHPRDYICRI VHLCAELVTA SDEEYQKAYD IVPLLHIRPV QNVCEELRRQ FRAGALTQRL PLGQRVDVQF KRTVVHLDGS MDPFPRNAAE AAVNIAPVAL DAVDDIYEGF DVTGTEVVDI PTGKVSEYLS EKDFELVTED SVLLDPTGKR VQAIFIRGGI DKDICRRAAA DVEGVATKQN MRRLTNGGVR NPDTGILGYY DYLNNPTKRK CRMTEFTRRN WGKIIGPCGE LLQLLDQLYK ENAPDHYELQ RRVIPPEYML FNTVFSTVSV NKNFRTAVHR DKGDFRGGLT ALCVLDGNYE GCYLALKSAR KAFCLQVGDV LFFDSSLEHG NTEVHNREGS WRRISIVCYL RCGLMSHTCE TERSMRLRNQ IMSDRLHADS ADSVVNLNGV TGHLPPLCIP FKIAKTLSLT QHAALRFVSR RIKEGDGCVL ALTMGLGKTL VSLTICYSYI YNNGPCDILI VAPKTLLQHW MQEAKKWKDY GLVFPGFIVL NNVDSSSFED DLSNYEQQGT TTNPKKSYVF VINPGYIKSF LSRVKGFRPA LIVVDEGHCI SSKESKLREV LDSLYCSARV VLTGTPVQNN AEELYRLVGW VDDKVHSTLP QRDFNEFSNS INRYVNGDDS AFCDALFAQR YIHEWMSPYV FTVMKVDLPP LHDYIIICNF SAVQQKMFEE RIKVDATDNL LCLKASEHRP YHLSTHPLCF LGFLTGIWRT GQVDIEEEPG EFEELGTYRL SRDDDALAKD CSSLLENGKL ADFVALSGKL TALISILHSI FEKMEKAVIF SQYIGSQDFI ARTLTAYKIS VVTIRGKDCQ QRRRRVVEMF RDDKNVLCLV VSTQIGAYGL DLTAANHVIL WDTWWNPQVE SQAIARCYRQ NQSKAVIAYK LASGFEDATV LKAQARKRAL FKCLINEETS QVVPGHDLVD YTSSEEDDDR RHLWETLKTC TLEGGKPAVT KIIRNIDTVK SERWI

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccta tgtttatgga tggggcgtct caagtgctgc agcaggtgct acaaactgtc ttagtaacga gtgagccagc gatcgttata cccggttcct tcttgggtga acttgatgtt atcgttgacg aggctaaaaa tcatggaatg aagttagtga gtatcccgaa aggtggcatc acgattctac caccaatacc gatgtcagaa tcttctctta ccaggctatg caaagactac tacgggttaa agactgatgc agagcgtttg gccctgttca gcaacctgga ggagacattc ccgacggctc caggtgtatc cttaccgtgc cgccttcttt accaccctcg cgattacata tgtcgaattg tccacctctg tgcggaactg gttaccgctt ctgatgagga atatcaaaaa gcgtacgata ttgttccgct tctgcacatc agacctgtgc aaaatgtatg tgaagaactt cgccgtcagt tccgagctgg tgctctcaca cagaggcttc ctctcggaca gcgcgtggac gttcaattca agaggactgt ggtccatcta gacggttcga tggatccatt ccctcgcaat gctgctgaag cagctgtaaa cattgcgcct gttgctctgg atgcagtgga tgatatttat gaaggctttg acgttaccgg taccgaggtg gttgacatcc ctacaggcaa ggtttcagag tacctctctg aaaaggattt tgagctggtg accgaggata gtgttttgct ggacccaacc ggaaagcgtg tgcaggccat ctttatacgt ggtggcattg ataaagatat ctgtcgacgt gccgcagctg atgtggaggg agtggctacc aaacaaaata tgcggagatt gacaaatggt ggtgtgagaa atccggacac gggtatcctt ggctattacg actatctcaa caatccaacg aagcgtaagt gccgcatgac tgagttcaca cgcaggaact ggggtaagat tatcggaccg tgtggtgagt tactgcagtt gttagatcaa ctatacaagg aaaacgcccc agaccattac gaacttcagc gcagggtcat accgcctgag tacatgctat tcaacaccgt tttcagtact gtaagtgtga acaagaactt tcgaaccgcc gttcatcgag acaaaggtga ctttcgcggt ggtcttactg cactctgcgt acttgacggt aattacgagg ggtgctacct agccttaaaa agtgcgcgca aggccttctg tcttcaggtt ggtgatgtct tatttttcga cagttctctg gaacacggaa acacggaagt acacaatcgt gaaggaagtt ggagacgaat cagcattgta tgctatctgc ggtgtgggtt gatgtcccac acatgtgaaa cggagcggag catgagactc cggaaccaga tcatgagcga tcgtctgcat gctgacagcg cagacagcgt ggtcaatttg aacggagtca ccggccacct tccacctttg tgtattcctt tcaaaatagc aaagaccctg agcttaactc agcatgcggc ccttagattt gtttcacgtc gtatcaagga aggagacggt tgtgtgctgg cgttaacaat gggacttggg aagacactgg tttcactgac gatctgctat tcttatattt acaacaatgg gccgtgtgac attctgatcg ttgcgccgaa gacactgctt caacactgga tgcaggaggc gaaaaagtgg aaggactatg gtctcgtatt cccggggttt attgtactaa acaacgtgga ctccagctcc ttcgaggatg atctcagtaa ctatgaacaa caaggcacaa ctactaaccc taagaagagt tacgtctttg tcatcaatcc tggctacatc aaatcatttc tttcgagggt aaaggggttc cgccccgctc tcatagtcgt tgatgaaggc cattgcatat cgtcgaagga aagtaaactg agggaggtac tggatagttt gtattgctcg gcaagggtag tactcactgg gacgccggtg cagaataacg ctgaggaact ctaccgtctt gttgggtggg tggatgacaa ggttcattcc acattgccgc aacgcgattt taacgagttt tcaaactcta tcaaccgcta tgttaacggt gatgatagtg ctttttgtga tgctctcttc gcacaacgat atatccacga gtggatgagc ccctatgtct ttaccgttat gaaggttgat cttccaccgc tgcacgacta cattattata tgcaactttt ccgctgtgca gcagaaaatg ttcgaggagc gcattaaagt cgatgcaaca gataacctat tgtgtctgaa ggcgtccgaa catcggccat accacctttc aacacatcca ctgtgtttct tgggtttttt gaccggtata tggagaacag gtcaagttga tatcgaagaa gaaccagggg agttcgaaga gttgggaacg tacaggctta gccgtgatga tgacgcttta gcgaaggatt gttcaagttt gcttgaaaac gggaaactgg ccgactttgt tgcgctcagt ggtaagctaa cagccctcat ttcgatttta cacagcattt tcgagaagat ggaaaaggct gtgatatttt cacaatacat tgggtcgcaa gacttcattg caagaacctt gacggcttat aagattagtg tagttacaat tcgtggaaaa gattgccagc aacgtcgacg aagggttgtt gagatgttca gggatgataa gaacgtactc tgcttggttg tgtccactca gattggcgcg tacggtcttg atttaactgc ggcgaatcac gttattctgt gggacacgtg gtggaatcct caggttgagt cacaggcaat tgcgcggtgc tacagacaaa atcagtcaaa ggctgttatt gcctataaat tagcatcggg attcgaagac gccacagttt taaaagccca agcaagaaaa agggcactgt tcaagtgcct cataaacgag gaaacctcac aggtggttcc tgggcacgat ttggttgact acacaagcag cgaagaagat gatgataggc gtcatctatg ggagacctta aaaacgtgta cccttgaggg gggaaaacct gctgtaacaa agatcattcg caatattgat actgtaaaga gcgagcggtg gatatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for TrbrA.19528.a.B3.GE41495

HARVESTED ON:	4/21/2017
SEQUENCED ON:	5/3/2017
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMD VTGTEVVDIP TGKVSEYLSE KDFELVTEDS VLLDPTGKRV QAIFIRGGID KDICRRAAAD VEGVATKQNM RRLTNGGVRN PDTGILGYYD YLNNPTKRKC RMTEFTRRNW GKIIGPCGEL LQLLDQLYKE NAPDHYELQR RVIPPEYMLF NTVFSTVSVN KNFRTAVHRD KGDFRGGLTA LCVLDGNYEG CYLALKSARK AFCLQVGDVL FFDSSLEHGN TEVHNREGSW RRISIVCYLR CGLMSHTCET ERS

Validated NT Sequence

atggctcacc accaccacca ccatatggac gttaccggta ccgaggtggt tgacatccct acaggcaagg tttcagagta cctctctgaa aaggattttg agctggtgac cgaggatagt gttttgctgg acccaaccgg aaagcgtgtg caggccatct ttatacgtgg tggcattgat aaagatatct gtcgacgtgc cgcagctgat gtggagggag tggctaccaa acaaaatatg cggagattga caaatggtgg tgtgagaaat ccggacacgg gtatccttgg ctattacgac tatctcaaca atccaacgaa gcgtaagtgc cgcatgactg agttcacacg caggaactgg ggtaagatta tcggaccgtg tggtgagtta ctgcagttgt tagatcaact atacaaggaa aacgccccag accattacga gcttcagcgc agggtcatac cgcctgagta catgctattc aacaccgttt tcagtactgt aagtgtgaac aagaactttc gaaccgccgt tcatcgagac aaaggtgact ttcgcggtgg tcttactgca ctctgcgtac ttgacggtaa ttacgagggg tgctacctag ccttaaaaag tgcgcgcaag gccttctgtc ttcaggttgg tgatgtctta tttttcgaca gttctctgga acacggaaac acggaagtac acaatcgtga aggaagttgg agacgaatca gcattgtatg ctatctgcgg tgtgggttga tgtcccacac atgtgaaacg gagcggagct aagtgagtaa gat

Expected Protein Sequence

MAHHHHHHDV TGTEVVDIPT GKVSEYLSEK DFELVTEDSV LLDPTGKRVQ AIFIRGGIDK DICRRAAADV EGVATKQNMR RLTNGGVRNP DTGILGYYDY LNNPTKRKCR MTEFTRRNWG KIIGPCGELL QLLDQLYKEN APDHYELQRR VIPPEYMLFN TVFSTVSVNK NFRTAVHRDK GDFRGGLTAL CVLDGNYEGC YLALKSARKA FCLQVGDVLF FDSSLEHGNT EVHNREGSWR RISIVCYLRC GLMSHTCETE RS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgacgtta ccggtaccga ggtggttgac atccctacag gcaaggtttc agagtacctc tctgaaaagg attttgagct ggtgaccgag gatagtgttt tgctggaccc aaccggaaag cgtgtgcagg ccatctttat acgtggtggc attgataaag atatctgtcg acgtgccgca gctgatgtgg agggagtggc taccaaacaa aatatgcgga gattgacaaa tggtggtgtg agaaatccgg acacgggtat ccttggctat tacgactatc tcaacaatcc aacgaagcgt aagtgccgca tgactgagtt cacacgcagg aactggggta agattatcgg accgtgtggt gagttactgc agttgttaga tcaactatac aaggaaaacg ccccagacca ttacgaactt cagcgcaggg tcataccgcc tgagtacatg ctattcaaca ccgttttcag tactgtaagt gtgaacaaga actttcgaac cgccgttcat cgagacaaag gtgactttcg cggtggtctt actgcactct gcgtacttga cggtaattac gaggggtgct acctagcctt aaaaagtgcg cgcaaggcct tctgtcttca ggttggtgat gtcttatttt tcgacagttc tctggaacac ggaaacacgg aagtacacaa tcgtgaagga agttggagac gaatcagcat tgtatgctat ctgcggtgtg ggttgatgtc ccacacatgt gaaacggagc ggagctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for TrbrA.19528.a.B3.PS38335

PURIFICATION DATe:	8/17/2017
CONCENTRATION:	28.2mg/ml
OBSERVED MW:	30kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	4
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGACGTTA CCGGTACCGA GGTGGTTGAC ATCCCTACAG GCAAGGTTTC AGAGTACCTC TCTGAAAAGG ATTTTGAGCT GGTGACCGAG GATAGTGTTT TGCTGGACCC AACCGGAAAG CGTGTGCAGG CCATCTTTAT ACGTGGTGGC ATTGATAAAG ATATCTGTCG ACGTGCCGCA GCTGATGTGG AGGGAGTGGC TACCAAACAA AATATGCGGA GATTGACAAA TGGTGGTGTG AGAAATCCGG ACACGGGTAT CCTTGGCTAT TACGACTATC TCAACAATCC AACGAAGCGT AAGTGCCGCA TGACTGAGTT CACACGCAGG AACTGGGGTA AGATTATCGG ACCGTGTGGT GAGTTACTGC AGTTGTTAGA TCAACTATAC AAGGAAAACG CCCCAGACCA TTACGAACTT CAGCGCAGGG TCATACCGCC TGAGTACATG CTATTCAACA CCGTTTTCAG TACTGTAAGT GTGAACAAGA ACTTTCGAAC CGCCGTTCAT CGAGACAAAG GTGACTTTCG CGGTGGTCTT ACTGCACTCT GCGTACTTGA CGGTAATTAC GAGGGGTGCT ACCTAGCCTT AAAAAGTGCG CGCAAGGCCT TCTGTCTTCA GGTTGGTGAT GTCTTATTTT TCGACAGTTC TCTGGAACAC GGAAACACGG AAGTACACAA TCGTGAAGGA AGTTGGAGAC GAATCAGCAT TGTATGCTAT CTGCGGTGTG GGTTGATGTC CCACACATGT GAAACGGAGC GGAGCTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT